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The map label for this gene is purF [H]

Identifier: 66044913

GI number: 66044913

Start: 1872455

End: 1873963

Strand: Direct

Name: purF [H]

Synonym: Psyr_1668

Alternate gene names: 66044913

Gene position: 1872455-1873963 (Clockwise)

Preceding gene: 66044912

Following gene: 66044914

Centisome position: 30.73

GC content: 59.58

Gene sequence:

>1509_bases
ATGTGTGGCATCGTCGGTATCGTCGGCAAGTCGAACGTCAATCAGGCGCTGTATGACGCGCTTACCGTCCTCCAGCACCG
CGGCCAGGACGCTGCCGGTATCGTAACCAGCCATGATGGCCGGTTATTCCTGCGCAAGGACAACGGATTGGTGCGCGATG
TGTTTCATCAACGTCATATGCAGCGTCTGGTCGGGCATATGGGCATCGGTCACGTGCGTTACCCCACCGCGGGCAGTTCG
ACGTCGGCCGAGGCTCAGCCGTTTTACGTCAACTCGCCTTACGGCATCACGCTGGCGCACAACGGCAACCTCACCAACGT
CGAGCAGCTGGCCAAGGAAATTTACGAGTCCGACCTGCGCCATGTGAACACCAATTCCGATTCGGAAGTGCTGCTCAACG
TGTTCGCCCATGAACTGGCCGTGCGTGGCAAGTTGCAGCCAACCGAAGAGGATATCTTCGCGGCCGTGACCGACGTGCAC
AATCGCTGCCGCGGTGGTTATGCGGTCGTAGCCATGATCACCGGCTACGGTATCGTCGGCTTCCGTGACCCGGACGCCAT
TCGTCCTATCGTGTTCGGCCAGCGTCACACCGACGAAGGCGTCGAGTACATGATCGCCTCGGAAAGCGTATCGCTGGACG
TGCTGGGCTTTACCCTGATTCGCGACCTTGCACCGGGCGAAGCGGTCTACATCACCGAAGACGGCAAGCTGCACACCCGT
CAGTGCGCGGCCAACCCCAAATACGCTCCGTGTATCTTCGAGCACGTCTATCTGGCGCGCCCGGATTCGATCATCGACGG
TATCTCGGTCTACAAGGCGCGCCTGCGCATGGGCGAGAAGCTGGCCGACAAGATCCTGCGCGAGCGTCCGGATCACGATA
TCGACGTGGTCATCCCGATTCCGGACACCAGCCGTACTGCGGCGCTGGAGCTGGCCAACCATCTGGGCGTCAAGTTCCGC
GAAGGCTTCGTCAAGAACCGTTACATCGGCCGGACCTTCATCATGCCGGGCCAGGCTGCGCGCAAGAAATCCGTACGCCA
GAAGCTCAACGCCATCGAGCTGGAGTTCCGCGGCAAGAACGTGATGCTGGTGGATGACTCCATCGTACGCGGCACGACCT
GCAAGCAGATCATCCAGATGGCCCGCGAAGCCGGTGCCAAGAATGTCTACTTCTGTTCTGCAGCCCCGGCTGTGCGCTAC
CCCAACGTCTACGGCATCGACATGCCGAGTGCTCACGAGCTGATTGCCCACAACCGCACCACGCAGGACGTGGCCGACCT
CATCGGTGCCGACTGGCTGGTCTATCAGGACCTGAATGACCTGATCGAAGCGGTCAGCGGCAGCAAGAAAATCAAGATCG
ACAACTTCGATTGCTCGGTTTTCGACGGCAAGTACGTGACCGGCGACATCGACGAGCATTATTTGAACCGGATCGAGAGC
GCGCGTAATGACGCCTCCAAGATCAAGACTCAGGCAGTGAGCGCGATCATCGATCTGTACAACAACTAA

Upstream 100 bases:

>100_bases
CGTACCCGCTGACATGCCGTTCAAGGAACAGATCCTGCCGTCTACAATGCCGCAGGATGTGCTCGGTAAATCCAGTTCCA
CTAAGTAGGGGTTGTGTCGC

Downstream 100 bases:

>100_bases
TTGGGCAAGGAGTGGGCGGCATGACTCAGGAATGGGATGCAGGTCGGCTGGACAGCGACCTTGAAGGCGTAGGTTTCGAT
ACCCTAGCCGTACGTGCCGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MCGIVGIVGKSNVNQALYDALTVLQHRGQDAAGIVTSHDGRLFLRKDNGLVRDVFHQRHMQRLVGHMGIGHVRYPTAGSS
TSAEAQPFYVNSPYGITLAHNGNLTNVEQLAKEIYESDLRHVNTNSDSEVLLNVFAHELAVRGKLQPTEEDIFAAVTDVH
NRCRGGYAVVAMITGYGIVGFRDPDAIRPIVFGQRHTDEGVEYMIASESVSLDVLGFTLIRDLAPGEAVYITEDGKLHTR
QCAANPKYAPCIFEHVYLARPDSIIDGISVYKARLRMGEKLADKILRERPDHDIDVVIPIPDTSRTAALELANHLGVKFR
EGFVKNRYIGRTFIMPGQAARKKSVRQKLNAIELEFRGKNVMLVDDSIVRGTTCKQIIQMAREAGAKNVYFCSAAPAVRY
PNVYGIDMPSAHELIAHNRTTQDVADLIGADWLVYQDLNDLIEAVSGSKKIKIDNFDCSVFDGKYVTGDIDEHYLNRIES
ARNDASKIKTQAVSAIIDLYNN

Sequences:

>Translated_502_residues
MCGIVGIVGKSNVNQALYDALTVLQHRGQDAAGIVTSHDGRLFLRKDNGLVRDVFHQRHMQRLVGHMGIGHVRYPTAGSS
TSAEAQPFYVNSPYGITLAHNGNLTNVEQLAKEIYESDLRHVNTNSDSEVLLNVFAHELAVRGKLQPTEEDIFAAVTDVH
NRCRGGYAVVAMITGYGIVGFRDPDAIRPIVFGQRHTDEGVEYMIASESVSLDVLGFTLIRDLAPGEAVYITEDGKLHTR
QCAANPKYAPCIFEHVYLARPDSIIDGISVYKARLRMGEKLADKILRERPDHDIDVVIPIPDTSRTAALELANHLGVKFR
EGFVKNRYIGRTFIMPGQAARKKSVRQKLNAIELEFRGKNVMLVDDSIVRGTTCKQIIQMAREAGAKNVYFCSAAPAVRY
PNVYGIDMPSAHELIAHNRTTQDVADLIGADWLVYQDLNDLIEAVSGSKKIKIDNFDCSVFDGKYVTGDIDEHYLNRIES
ARNDASKIKTQAVSAIIDLYNN
>Mature_502_residues
MCGIVGIVGKSNVNQALYDALTVLQHRGQDAAGIVTSHDGRLFLRKDNGLVRDVFHQRHMQRLVGHMGIGHVRYPTAGSS
TSAEAQPFYVNSPYGITLAHNGNLTNVEQLAKEIYESDLRHVNTNSDSEVLLNVFAHELAVRGKLQPTEEDIFAAVTDVH
NRCRGGYAVVAMITGYGIVGFRDPDAIRPIVFGQRHTDEGVEYMIASESVSLDVLGFTLIRDLAPGEAVYITEDGKLHTR
QCAANPKYAPCIFEHVYLARPDSIIDGISVYKARLRMGEKLADKILRERPDHDIDVVIPIPDTSRTAALELANHLGVKFR
EGFVKNRYIGRTFIMPGQAARKKSVRQKLNAIELEFRGKNVMLVDDSIVRGTTCKQIIQMAREAGAKNVYFCSAAPAVRY
PNVYGIDMPSAHELIAHNRTTQDVADLIGADWLVYQDLNDLIEAVSGSKKIKIDNFDCSVFDGKYVTGDIDEHYLNRIES
ARNDASKIKTQAVSAIIDLYNN

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=473, Percent_Identity=35.0951374207188, Blast_Score=250, Evalue=2e-66,
Organism=Escherichia coli, GI1788651, Length=490, Percent_Identity=65.3061224489796, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=200, Percent_Identity=29, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554892, Length=459, Percent_Identity=33.7690631808279, Blast_Score=236, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6323958, Length=513, Percent_Identity=53.2163742690059, Blast_Score=526, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24659598, Length=463, Percent_Identity=35.4211663066955, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24659604, Length=455, Percent_Identity=35.8241758241758, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI28573187, Length=504, Percent_Identity=33.3333333333333, Blast_Score=242, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55654; Mature: 55654

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGKSNVNQALYDALTVLQHRGQDAAGIVTSHDGRLFLRKDNGLVRDVFHQRHM
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHH
QRLVGHMGIGHVRYPTAGSSTSAEAQPFYVNSPYGITLAHNGNLTNVEQLAKEIYESDLR
HHHHHHCCCCCEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HVNTNSDSEVLLNVFAHELAVRGKLQPTEEDIFAAVTDVHNRCRGGYAVVAMITGYGIVG
CCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEE
FRDPDAIRPIVFGQRHTDEGVEYMIASESVSLDVLGFTLIRDLAPGEAVYITEDGKLHTR
ECCCCCCCCEEECCCCCCCCCEEEEECCCCEEEHHHHHHHHHCCCCCEEEEECCCCEEHH
QCAANPKYAPCIFEHVYLARPDSIIDGISVYKARLRMGEKLADKILRERPDHDIDVVIPI
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PDTSRTAALELANHLGVKFREGFVKNRYIGRTFIMPGQAARKKSVRQKLNAIELEFRGKN
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCEEEEEECCCE
VMLVDDSIVRGTTCKQIIQMAREAGAKNVYFCSAAPAVRYPNVYGIDMPSAHELIAHNRT
EEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEECCCCCHHHHHHCCCC
TQDVADLIGADWLVYQDLNDLIEAVSGSKKIKIDNFDCSVFDGKYVTGDIDEHYLNRIES
HHHHHHHHCCCEEEHHHHHHHHHHHCCCCEEEEECCCEEEECCEEEECCCHHHHHHHHHH
ARNDASKIKTQAVSAIIDLYNN
HCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGIVGIVGKSNVNQALYDALTVLQHRGQDAAGIVTSHDGRLFLRKDNGLVRDVFHQRHM
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHH
QRLVGHMGIGHVRYPTAGSSTSAEAQPFYVNSPYGITLAHNGNLTNVEQLAKEIYESDLR
HHHHHHCCCCCEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HVNTNSDSEVLLNVFAHELAVRGKLQPTEEDIFAAVTDVHNRCRGGYAVVAMITGYGIVG
CCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEE
FRDPDAIRPIVFGQRHTDEGVEYMIASESVSLDVLGFTLIRDLAPGEAVYITEDGKLHTR
ECCCCCCCCEEECCCCCCCCCEEEEECCCCEEEHHHHHHHHHCCCCCEEEEECCCCEEHH
QCAANPKYAPCIFEHVYLARPDSIIDGISVYKARLRMGEKLADKILRERPDHDIDVVIPI
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PDTSRTAALELANHLGVKFREGFVKNRYIGRTFIMPGQAARKKSVRQKLNAIELEFRGKN
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCEEEEEECCCE
VMLVDDSIVRGTTCKQIIQMAREAGAKNVYFCSAAPAVRYPNVYGIDMPSAHELIAHNRT
EEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEECCCCCHHHHHHCCCC
TQDVADLIGADWLVYQDLNDLIEAVSGSKKIKIDNFDCSVFDGKYVTGDIDEHYLNRIES
HHHHHHHHCCCEEEHHHHHHHHHHHCCCCEEEEECCCEEEECCEEEECCCHHHHHHHHHH
ARNDASKIKTQAVSAIIDLYNN
HCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]