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The map label for this gene is truA [H]

Identifier: 66044907

GI number: 66044907

Start: 1867089

End: 1867943

Strand: Direct

Name: truA [H]

Synonym: Psyr_1662

Alternate gene names: 66044907

Gene position: 1867089-1867943 (Clockwise)

Preceding gene: 66044906

Following gene: 66044908

Centisome position: 30.64

GC content: 60.47

Gene sequence:

>855_bases
TTGGCGAATATAGATAACCCGGCCGCCGAGATGGCGGCCGAGGGCTTTTCCCGAATCGCGCTGGGCGTTGAATACAAGGG
CTCGCGCTATTGCGGCTGGCAGCGTCAGGCGTCCGGCGTGCTGACCGTGCAGGAAACCCTTGAAGACGCGTTGTCAAAAG
TGGCCGCGTCACCGGTTTCACTGATGTGTGCCGGTCGCACGGACGCGGGCGTGCATGCCTGCGGTCAGGTGGTGCATTTC
GACACCCAGGCCGAGCGCAGCATGAAGGCCTGGGTCATGGGCGCCAATATCAATCTGCCCCATGATGTCAGTGTCACCTG
GGCCAGAGTCATGCCCGCCACCTTTCATGCGCGCTTCAAGGCCATTGCCCGGCGTTATCGTTACGTGATCTACAACGATC
AGATCCGTCCGGCGCACCTGAACCAGGAAATTACCTGGAACCACCGCCCGCTGGATGTCGAGCGCATGGCGCAGGCTGCC
GAATATCTGGTGGGTACTCACGATTTCAGCGCCTTTCGCGCCGGTCAGTGTCAGGCCAAGTCGCCGGTCAAGCAGTTGCA
CCATCTGCGCGTGACCCGTCACGGCAAGATGATCGTCATTGATGTGCGCGCCAATGCCTTTCTTCATCATATGGTGCGCA
ATATCGCCGGTGTACTGATGACCATCGGTACCGGAGAGCGACCGATCGAGTGGGCGCGGGAGGTGCTGGAAAGCCGGATT
CGGCGCACCGGTGGTGTCACGGCCCATCCTTACGGGTTGTATCTGGTCGGGGTTGAGTACCGTGATGAATTCCCGCTGCC
CGAGCGCTATATCGGCCCCCATTTTCTTACGGGCTTTGCGGAATTGGACGGCTGA

Upstream 100 bases:

>100_bases
TTATGCGGCCGTTTGCATTTCAGATGTCCCGCTTGAGTAGCGTGTTTTTTTTGCGGCCGTATAATGGCCGACTTTTCGCG
AACCGACAGGCTGTACCACT

Downstream 100 bases:

>100_bases
CGATCCGGGCCGCATTTGCTAACATCCGGGACTTTCCGAAAGGCCTGAGGTTACTCACGACATGTCAGCCGTTCGCAGCA
AGATCTGCGGGATTACCCGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MANIDNPAAEMAAEGFSRIALGVEYKGSRYCGWQRQASGVLTVQETLEDALSKVAASPVSLMCAGRTDAGVHACGQVVHF
DTQAERSMKAWVMGANINLPHDVSVTWARVMPATFHARFKAIARRYRYVIYNDQIRPAHLNQEITWNHRPLDVERMAQAA
EYLVGTHDFSAFRAGQCQAKSPVKQLHHLRVTRHGKMIVIDVRANAFLHHMVRNIAGVLMTIGTGERPIEWAREVLESRI
RRTGGVTAHPYGLYLVGVEYRDEFPLPERYIGPHFLTGFAELDG

Sequences:

>Translated_284_residues
MANIDNPAAEMAAEGFSRIALGVEYKGSRYCGWQRQASGVLTVQETLEDALSKVAASPVSLMCAGRTDAGVHACGQVVHF
DTQAERSMKAWVMGANINLPHDVSVTWARVMPATFHARFKAIARRYRYVIYNDQIRPAHLNQEITWNHRPLDVERMAQAA
EYLVGTHDFSAFRAGQCQAKSPVKQLHHLRVTRHGKMIVIDVRANAFLHHMVRNIAGVLMTIGTGERPIEWAREVLESRI
RRTGGVTAHPYGLYLVGVEYRDEFPLPERYIGPHFLTGFAELDG
>Mature_283_residues
ANIDNPAAEMAAEGFSRIALGVEYKGSRYCGWQRQASGVLTVQETLEDALSKVAASPVSLMCAGRTDAGVHACGQVVHFD
TQAERSMKAWVMGANINLPHDVSVTWARVMPATFHARFKAIARRYRYVIYNDQIRPAHLNQEITWNHRPLDVERMAQAAE
YLVGTHDFSAFRAGQCQAKSPVKQLHHLRVTRHGKMIVIDVRANAFLHHMVRNIAGVLMTIGTGERPIEWAREVLESRIR
RTGGVTAHPYGLYLVGVEYRDEFPLPERYIGPHFLTGFAELDG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=267, Percent_Identity=28.4644194756554, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1788657, Length=259, Percent_Identity=53.6679536679537, Blast_Score=298, Evalue=4e-82,
Organism=Saccharomyces cerevisiae, GI6321112, Length=234, Percent_Identity=26.4957264957265, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 31820; Mature: 31689

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIDNPAAEMAAEGFSRIALGVEYKGSRYCGWQRQASGVLTVQETLEDALSKVAASPVS
CCCCCCHHHHHHHHHHHHEEEEEEECCCCCCCCHHHCCCCEEHHHHHHHHHHHHHCCCEE
LMCAGRTDAGVHACGQVVHFDTQAERSMKAWVMGANINLPHDVSVTWARVMPATFHARFK
EEEECCCCCCHHHHCCEEEECCHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
AIARRYRYVIYNDQIRPAHLNQEITWNHRPLDVERMAQAAEYLVGTHDFSAFRAGQCQAK
HHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCC
SPVKQLHHLRVTRHGKMIVIDVRANAFLHHMVRNIAGVLMTIGTGERPIEWAREVLESRI
CHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHH
RRTGGVTAHPYGLYLVGVEYRDEFPLPERYIGPHFLTGFAELDG
HHHCCCCCCCCEEEEEEEEECCCCCCCHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
ANIDNPAAEMAAEGFSRIALGVEYKGSRYCGWQRQASGVLTVQETLEDALSKVAASPVS
CCCCCHHHHHHHHHHHHEEEEEEECCCCCCCCHHHCCCCEEHHHHHHHHHHHHHCCCEE
LMCAGRTDAGVHACGQVVHFDTQAERSMKAWVMGANINLPHDVSVTWARVMPATFHARFK
EEEECCCCCCHHHHCCEEEECCHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
AIARRYRYVIYNDQIRPAHLNQEITWNHRPLDVERMAQAAEYLVGTHDFSAFRAGQCQAK
HHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCC
SPVKQLHHLRVTRHGKMIVIDVRANAFLHHMVRNIAGVLMTIGTGERPIEWAREVLESRI
CHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHH
RRTGGVTAHPYGLYLVGVEYRDEFPLPERYIGPHFLTGFAELDG
HHHCCCCCCCCEEEEEEEEECCCCCCCHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]