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The map label for this gene is iaaH [H]

Identifier: 66044783

GI number: 66044783

Start: 1727496

End: 1728836

Strand: Direct

Name: iaaH [H]

Synonym: Psyr_1537

Alternate gene names: 66044783

Gene position: 1727496-1728836 (Clockwise)

Preceding gene: 66044782

Following gene: 66044784

Centisome position: 28.35

GC content: 54.21

Gene sequence:

>1341_bases
ATGCGCGAAATGATCACACTTGAATCGCTGTGCCGGGCATTGGCCGATGAACAGATTGCGGCTGAAGAGCTGCGCGGGCG
GGCGCTCGACACTGAGGCCCGCTTGACCCTTTTGAACTGTTTCATACGCGAGGGCGATGCGGTCAGCCAGTTTGGCGAGG
CTGATCAAGCAAGGAAGGGTACTTCACTCTGGGGCGTGCCAGTTTCCTTCAAGGATAATATCTGTGTTCGCGGTCTGCCG
TTGACGGCCGGGACGCGAGGGATGTCCGGTTTCATAGCTGATCAGGATGCTGCGATTGTCAGTCAACTCAAAGCCCTCGG
GGCTGTGGTTGCTGGCAAGAACAATATGCACGAACTTAGTTTCGGGGTGACATCCATCAATCCTCATTGGGGAGCCGTGG
GAAACCCCGTTGCCCCCGGGTATTGTGCCGGGGGCAGTAGTGGTGGAAGTGCCGCCGCAGTGGCAAGTGGAATCGTTCCG
CTGTCGGTGGGGACCGATACGGGGGGCTCGATAAGAATACCGGCGGCTTTTTGCGGCATTACGGGCTTCAGACCCACTAC
TGGACGCTTGTCAACGGCAGGCATTATCCCTGTTTCTCATACAAAGGATTGCGTTGGGTTACTGACACGAACAGCAGGCG
ACGCCGAATTTGTGTATGGGCTGTTGTCAGGCAAGCAGCAGTCTTTTCCTCTGAGCAGGACGGGTCCCTGTCGAATCGGC
CTACCGGTCTCCATGTGGTCTGATCTGGATGGCGAGGTGGAGAGGGCATGCGTTAATGCACTCAGCCTGCTGCGCAAGAC
AGGGTTCGAGTTTGTTGAAATTGATGATGCCGATATTGTGGAACTGAACCAGACACTCACGTTCACCGTTCCGCTTTACG
AATTCTTTGCCGACTTTGCTCAGTCATTGCTCTCCTTGGGCTGGAAGCACGGTATCCATCATATTTTTGCGCAGGTTGAT
GATGCCAACGTAAAAGGCATCATCAATCACCATCTTGGAGAGGGCGCTATAAAACCGGCTCACTATTTGAGTTCACTGCA
AAATGGCGAGCTGTTGAAAAGAAAAATGGATGAGCTATTTGCTCGTCATCATATCAAGCTCCTGGGGTATCCCACGGTAC
CTTGCCGGGTACCTCATCTGGATCATGCTGACCGACCAGAGTTTTTCAGTCAGGCCATTCGCAATACCGACCTGGCCAGC
AACGCGATGCTCCCCTCTATAACGATTCCCGTCGGGCCGGAAGGGCGGTTGCCGGTCGGCTTGAGCTTTGACGCGCCCAG
AGCGCGAGATGCTTTTTTACTGAGCAACGTGAGTCTTATAGAGAAAGTATTAAAGGGTTGA

Upstream 100 bases:

>100_bases
CACCGGAGGGCAGGTGTCAGAAGGCAACCCGCTTGACTGTATTAACGCCTCCTATCGCTATTAGCAGTCCTGAGAGAAGC
GCTTATACAGGGAGAAGAGT

Downstream 100 bases:

>100_bases
GGTTTCAGTTGCCGACGCTTGTTGACGGGGTGTCGCGGTGTCGGCGTTTATCTACGCAAACCGACGTCAGGAGTTTCGAT
GAGCGTTTCCGAAAGACGCG

Product: amidase

Products: NA

Alternate protein names: IAH; Indole-3-acetamide hydrolase [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MREMITLESLCRALADEQIAAEELRGRALDTEARLTLLNCFIREGDAVSQFGEADQARKGTSLWGVPVSFKDNICVRGLP
LTAGTRGMSGFIADQDAAIVSQLKALGAVVAGKNNMHELSFGVTSINPHWGAVGNPVAPGYCAGGSSGGSAAAVASGIVP
LSVGTDTGGSIRIPAAFCGITGFRPTTGRLSTAGIIPVSHTKDCVGLLTRTAGDAEFVYGLLSGKQQSFPLSRTGPCRIG
LPVSMWSDLDGEVERACVNALSLLRKTGFEFVEIDDADIVELNQTLTFTVPLYEFFADFAQSLLSLGWKHGIHHIFAQVD
DANVKGIINHHLGEGAIKPAHYLSSLQNGELLKRKMDELFARHHIKLLGYPTVPCRVPHLDHADRPEFFSQAIRNTDLAS
NAMLPSITIPVGPEGRLPVGLSFDAPRARDAFLLSNVSLIEKVLKG

Sequences:

>Translated_446_residues
MREMITLESLCRALADEQIAAEELRGRALDTEARLTLLNCFIREGDAVSQFGEADQARKGTSLWGVPVSFKDNICVRGLP
LTAGTRGMSGFIADQDAAIVSQLKALGAVVAGKNNMHELSFGVTSINPHWGAVGNPVAPGYCAGGSSGGSAAAVASGIVP
LSVGTDTGGSIRIPAAFCGITGFRPTTGRLSTAGIIPVSHTKDCVGLLTRTAGDAEFVYGLLSGKQQSFPLSRTGPCRIG
LPVSMWSDLDGEVERACVNALSLLRKTGFEFVEIDDADIVELNQTLTFTVPLYEFFADFAQSLLSLGWKHGIHHIFAQVD
DANVKGIINHHLGEGAIKPAHYLSSLQNGELLKRKMDELFARHHIKLLGYPTVPCRVPHLDHADRPEFFSQAIRNTDLAS
NAMLPSITIPVGPEGRLPVGLSFDAPRARDAFLLSNVSLIEKVLKG
>Mature_446_residues
MREMITLESLCRALADEQIAAEELRGRALDTEARLTLLNCFIREGDAVSQFGEADQARKGTSLWGVPVSFKDNICVRGLP
LTAGTRGMSGFIADQDAAIVSQLKALGAVVAGKNNMHELSFGVTSINPHWGAVGNPVAPGYCAGGSSGGSAAAVASGIVP
LSVGTDTGGSIRIPAAFCGITGFRPTTGRLSTAGIIPVSHTKDCVGLLTRTAGDAEFVYGLLSGKQQSFPLSRTGPCRIG
LPVSMWSDLDGEVERACVNALSLLRKTGFEFVEIDDADIVELNQTLTFTVPLYEFFADFAQSLLSLGWKHGIHHIFAQVD
DANVKGIINHHLGEGAIKPAHYLSSLQNGELLKRKMDELFARHHIKLLGYPTVPCRVPHLDHADRPEFFSQAIRNTDLAS
NAMLPSITIPVGPEGRLPVGLSFDAPRARDAFLLSNVSLIEKVLKG

Specific function: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid (IAA) [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=509, Percent_Identity=25.5402750491159, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI195972892, Length=192, Percent_Identity=32.8125, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI166795287, Length=196, Percent_Identity=35.7142857142857, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=408, Percent_Identity=27.2058823529412, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=274, Percent_Identity=29.1970802919708, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=197, Percent_Identity=35.5329949238579, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=439, Percent_Identity=25.7403189066059, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=173, Percent_Identity=31.7919075144509, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=275, Percent_Identity=27.6363636363636, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=275, Percent_Identity=27.6363636363636, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI32565337, Length=301, Percent_Identity=27.2425249169435, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=179, Percent_Identity=28.4916201117318, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=270, Percent_Identity=31.8518518518519, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323950, Length=180, Percent_Identity=37.2222222222222, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648113, Length=231, Percent_Identity=33.7662337662338, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356731, Length=165, Percent_Identity=36.3636363636364, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45550774, Length=188, Percent_Identity=34.5744680851064, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648435, Length=188, Percent_Identity=34.5744680851064, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648437, Length=188, Percent_Identity=34.5744680851064, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648441, Length=168, Percent_Identity=35.7142857142857, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648439, Length=168, Percent_Identity=35.7142857142857, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24644968, Length=433, Percent_Identity=24.4803695150115, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652985, Length=208, Percent_Identity=28.3653846153846, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922090, Length=208, Percent_Identity=28.3653846153846, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24652981, Length=208, Percent_Identity=28.3653846153846, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24652983, Length=208, Percent_Identity=28.3653846153846, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI161078093, Length=194, Percent_Identity=28.8659793814433, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 47595; Mature: 47595

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREMITLESLCRALADEQIAAEELRGRALDTEARLTLLNCFIREGDAVSQFGEADQARKG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHCC
TSLWGVPVSFKDNICVRGLPLTAGTRGMSGFIADQDAAIVSQLKALGAVVAGKNNMHELS
CCEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
FGVTSINPHWGAVGNPVAPGYCAGGSSGGSAAAVASGIVPLSVGTDTGGSIRIPAAFCGI
ECEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCEECCHHHHCC
TGFRPTTGRLSTAGIIPVSHTKDCVGLLTRTAGDAEFVYGLLSGKQQSFPLSRTGPCRIG
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCEEEC
LPVSMWSDLDGEVERACVNALSLLRKTGFEFVEIDDADIVELNQTLTFTVPLYEFFADFA
CCHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCEEEEEEHHHHHHHHHH
QSLLSLGWKHGIHHIFAQVDDANVKGIINHHLGEGAIKPAHYLSSLQNGELLKRKMDELF
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
ARHHIKLLGYPTVPCRVPHLDHADRPEFFSQAIRNTDLASNAMLPSITIPVGPEGRLPVG
HHHCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEE
LSFDAPRARDAFLLSNVSLIEKVLKG
ECCCCCCCCCHHHHHCHHHHHHHHCC
>Mature Secondary Structure
MREMITLESLCRALADEQIAAEELRGRALDTEARLTLLNCFIREGDAVSQFGEADQARKG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHCC
TSLWGVPVSFKDNICVRGLPLTAGTRGMSGFIADQDAAIVSQLKALGAVVAGKNNMHELS
CCEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
FGVTSINPHWGAVGNPVAPGYCAGGSSGGSAAAVASGIVPLSVGTDTGGSIRIPAAFCGI
ECEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCEECCHHHHCC
TGFRPTTGRLSTAGIIPVSHTKDCVGLLTRTAGDAEFVYGLLSGKQQSFPLSRTGPCRIG
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCEEEC
LPVSMWSDLDGEVERACVNALSLLRKTGFEFVEIDDADIVELNQTLTFTVPLYEFFADFA
CCHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCEEEEEEHHHHHHHHHH
QSLLSLGWKHGIHHIFAQVDDANVKGIINHHLGEGAIKPAHYLSSLQNGELLKRKMDELF
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
ARHHIKLLGYPTVPCRVPHLDHADRPEFFSQAIRNTDLASNAMLPSITIPVGPEGRLPVG
HHHCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEE
LSFDAPRARDAFLLSNVSLIEKVLKG
ECCCCCCCCCHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA