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The map label for this gene is yeiE [H]

Identifier: 66044634

GI number: 66044634

Start: 1568079

End: 1569005

Strand: Reverse

Name: yeiE [H]

Synonym: Psyr_1386

Alternate gene names: 66044634

Gene position: 1569005-1568079 (Counterclockwise)

Preceding gene: 66044636

Following gene: 66044629

Centisome position: 25.75

GC content: 60.95

Gene sequence:

>927_bases
ATGCGATTTACTCTCCGTCAGCTCCAGGTATTTGTGGCCGTCGCCCGGCAGGAAAGCGTCTCCAAAGCCGCAGTACTGCT
TTCGCTTTCGCAGTCGGCTGCCAGCACCTCCATCACCGAGCTGGAGCGCCAATCGAGCTGCCAGCTATTTGACCGTGCCG
GCAAGCGCCTCAGCCTCAACGCCACCGGCAGACAATTGCTGCCCCAAGCCGTCGCGCTGCTCGATCAGGCCAAGGAAATC
GAAGACCTGCTCAACGGCAAATCAGGCTTCGGTTCACTGGCGGTCGGGGCGACGCTGACGATCGGCAATTACCTGGCGAC
CCTGCTGATCGGCGGCTACATGCAGCGCCATCCGGAAAGCCAGGTCAAGCTGCATGTCCAGAACACGGCGCATATCGTGC
AACAGGTTGCGCACTACGAGATCGATCTGGGCCTCATCGAAGGCGATTGCAGCCACCCGGACATCGAAGTGCAATCCTGG
GTCGAAGACGAACTTGTGGTGTTCTGCGCCCCGCAGCATCCGCTGGCCAAGCGCGCCCAGGTAACCCTGGAAGAGCTGAC
CCATGAAGCCTGGATACTTCGGGAACAGGGCTCAGGCACACGCCTGACGTTCGATCAGGCGATGCGTCATCACCGTAACG
GGCTGAATGTCCGACTGGAGCTCGAGCACACCGAAGCCATCAAACGCGCAGTCGAATCCGGGCTGGGCATTGGCTGCATT
TCCAGACTGGCGCTGCGCGACGCCTTTCGTCGCGGCAATCTGGTCGAACTGGCCACGCCGGAGCTGGACCTGGCCAGGCA
GTTCTACTTCATCTGGCACAAACAGAAATACCAGACCTCGGCCATGCGCGAGTTTCTCGAACTGTGTCGCGCATTCACCG
CCGGGGTGCAGCGCAGCGACGAGATCGTGCTGCCCAGCCTGTATTGA

Upstream 100 bases:

>100_bases
TAGTGCGCGCAGAAGCACTGCGCTATTGCGCGACATTCTAGTGGCAGACACAATATCTGTTAAATGAATTATCAAGATAT
AGGTTATCGGTTATATAGAT

Downstream 100 bases:

>100_bases
CGATCTGTACCAAAGGCGTCAGCCCAGCAGAATGATCGCCCACACCAGACCAATCATGCTCAGGGCGATGAACTGGGCAG
CACTGCCCATGTCCTTGGCG

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRFTLRQLQVFVAVARQESVSKAAVLLSLSQSAASTSITELERQSSCQLFDRAGKRLSLNATGRQLLPQAVALLDQAKEI
EDLLNGKSGFGSLAVGATLTIGNYLATLLIGGYMQRHPESQVKLHVQNTAHIVQQVAHYEIDLGLIEGDCSHPDIEVQSW
VEDELVVFCAPQHPLAKRAQVTLEELTHEAWILREQGSGTRLTFDQAMRHHRNGLNVRLELEHTEAIKRAVESGLGIGCI
SRLALRDAFRRGNLVELATPELDLARQFYFIWHKQKYQTSAMREFLELCRAFTAGVQRSDEIVLPSLY

Sequences:

>Translated_308_residues
MRFTLRQLQVFVAVARQESVSKAAVLLSLSQSAASTSITELERQSSCQLFDRAGKRLSLNATGRQLLPQAVALLDQAKEI
EDLLNGKSGFGSLAVGATLTIGNYLATLLIGGYMQRHPESQVKLHVQNTAHIVQQVAHYEIDLGLIEGDCSHPDIEVQSW
VEDELVVFCAPQHPLAKRAQVTLEELTHEAWILREQGSGTRLTFDQAMRHHRNGLNVRLELEHTEAIKRAVESGLGIGCI
SRLALRDAFRRGNLVELATPELDLARQFYFIWHKQKYQTSAMREFLELCRAFTAGVQRSDEIVLPSLY
>Mature_308_residues
MRFTLRQLQVFVAVARQESVSKAAVLLSLSQSAASTSITELERQSSCQLFDRAGKRLSLNATGRQLLPQAVALLDQAKEI
EDLLNGKSGFGSLAVGATLTIGNYLATLLIGGYMQRHPESQVKLHVQNTAHIVQQVAHYEIDLGLIEGDCSHPDIEVQSW
VEDELVVFCAPQHPLAKRAQVTLEELTHEAWILREQGSGTRLTFDQAMRHHRNGLNVRLELEHTEAIKRAVESGLGIGCI
SRLALRDAFRRGNLVELATPELDLARQFYFIWHKQKYQTSAMREFLELCRAFTAGVQRSDEIVLPSLY

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=289, Percent_Identity=45.6747404844291, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI145693105, Length=252, Percent_Identity=29.3650793650794, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788748, Length=296, Percent_Identity=27.7027027027027, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787879, Length=295, Percent_Identity=27.4576271186441, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI157672245, Length=297, Percent_Identity=27.6094276094276, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788296, Length=297, Percent_Identity=27.6094276094276, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787530, Length=248, Percent_Identity=27.4193548387097, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082132, Length=296, Percent_Identity=26.0135135135135, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081904, Length=286, Percent_Identity=26.2237762237762, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787601, Length=195, Percent_Identity=26.1538461538462, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790399, Length=301, Percent_Identity=25.5813953488372, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788887, Length=185, Percent_Identity=27.5675675675676, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789506, Length=298, Percent_Identity=21.1409395973154, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=22.7118644067797, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34455; Mature: 34455

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTLRQLQVFVAVARQESVSKAAVLLSLSQSAASTSITELERQSSCQLFDRAGKRLSLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
ATGRQLLPQAVALLDQAKEIEDLLNGKSGFGSLAVGATLTIGNYLATLLIGGYMQRHPES
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
QVKLHVQNTAHIVQQVAHYEIDLGLIEGDCSHPDIEVQSWVEDELVVFCAPQHPLAKRAQ
CEEEEEHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHCCCCCEEEEECCCCCHHHHHH
VTLEELTHEAWILREQGSGTRLTFDQAMRHHRNGLNVRLELEHTEAIKRAVESGLGIGCI
HHHHHHHHHHEEEEECCCCCEEEHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCHHHH
SRLALRDAFRRGNLVELATPELDLARQFYFIWHKQKYQTSAMREFLELCRAFTAGVQRSD
HHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EIVLPSLY
CEECCCCC
>Mature Secondary Structure
MRFTLRQLQVFVAVARQESVSKAAVLLSLSQSAASTSITELERQSSCQLFDRAGKRLSLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
ATGRQLLPQAVALLDQAKEIEDLLNGKSGFGSLAVGATLTIGNYLATLLIGGYMQRHPES
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
QVKLHVQNTAHIVQQVAHYEIDLGLIEGDCSHPDIEVQSWVEDELVVFCAPQHPLAKRAQ
CEEEEEHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHCCCCCEEEEECCCCCHHHHHH
VTLEELTHEAWILREQGSGTRLTFDQAMRHHRNGLNVRLELEHTEAIKRAVESGLGIGCI
HHHHHHHHHHEEEEECCCCCEEEHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCHHHH
SRLALRDAFRRGNLVELATPELDLARQFYFIWHKQKYQTSAMREFLELCRAFTAGVQRSD
HHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EIVLPSLY
CEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]