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The map label for this gene is pcm [H]

Identifier: 66044620

GI number: 66044620

Start: 1554845

End: 1555522

Strand: Direct

Name: pcm [H]

Synonym: Psyr_1372

Alternate gene names: 66044620

Gene position: 1554845-1555522 (Clockwise)

Preceding gene: 66044619

Following gene: 66044621

Centisome position: 25.52

GC content: 60.62

Gene sequence:

>678_bases
ATGTCACGCGAGCAGGATGATTTATTGCGTCGAGGGATCGGAATGACGTCGCAGCGTACCCGCGAGCGTCTGATTCAACG
CCTCTGTGAAGAGGGCATTTCCAATCAGCGGGTACTGGACGTCATTCGCAAGACGCCCCGGCATCTGTTTGTCGATGAAG
CGCTGGCGCATCGCGCCTACGAAGACACCGCATTGCCCATCGGCCACAACCAGACGATATCCCAGCCCTACATGGTCGCG
CGGATGAGCGAGCTGCTGCTGGCAGCAGGGCCGCTGGACAAGGTCATGGAGATCGGTACAGGGTCCGGCTATCAGACCGC
TGTGCTCGCCCAGCTGGTAGAGCGAGTGTTTTCGGTCGAGCGCATCAAGGGGCTGCAGGATCGCGCCAAGGAGCGTCTGG
TCGAGCTCAATCTGCGCAACGTGGTTTTTCGCTGGGGTGACGGCTGGGAGGGCTGGCCAGCCCTGGCGCCGTATAACGGC
ATCATCGTCACGGCGGTGGCAACCGATGTACCGCAGGCATTGCTCGATCAACTGGCACCGGGAGGCCGTCTGGTCATCCC
TGTTGGCTCTGGGGAGGTTCAGCAGTTGATGCTGATCATTCGTGAAGAAAACGGTTTTTCCCGGCATGTGCTGGGGGCTG
TGCGCTTCGTACCCTTGCTCAACGGGCCCATCGCTTGA

Upstream 100 bases:

>100_bases
CGGTCGTACAAGGCTACGTATCGATCACGCCGTTGCAACTGGATAGAACCTGTCAGGACGGATTCAGCAGCCTGAACACC
TGGCTGGAGGGACTTCGTTG

Downstream 100 bases:

>100_bases
AGATTGTCTGCGGTAAACAATGAATTCTGGCGTCAGTCATCGGTCTACAGCGCGCGGCCCGGAATCTTGAATGGCCTGAT
GGCATCATTTCCACGCAGCT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSREQDDLLRRGIGMTSQRTRERLIQRLCEEGISNQRVLDVIRKTPRHLFVDEALAHRAYEDTALPIGHNQTISQPYMVA
RMSELLLAAGPLDKVMEIGTGSGYQTAVLAQLVERVFSVERIKGLQDRAKERLVELNLRNVVFRWGDGWEGWPALAPYNG
IIVTAVATDVPQALLDQLAPGGRLVIPVGSGEVQQLMLIIREENGFSRHVLGAVRFVPLLNGPIA

Sequences:

>Translated_225_residues
MSREQDDLLRRGIGMTSQRTRERLIQRLCEEGISNQRVLDVIRKTPRHLFVDEALAHRAYEDTALPIGHNQTISQPYMVA
RMSELLLAAGPLDKVMEIGTGSGYQTAVLAQLVERVFSVERIKGLQDRAKERLVELNLRNVVFRWGDGWEGWPALAPYNG
IIVTAVATDVPQALLDQLAPGGRLVIPVGSGEVQQLMLIIREENGFSRHVLGAVRFVPLLNGPIA
>Mature_224_residues
SREQDDLLRRGIGMTSQRTRERLIQRLCEEGISNQRVLDVIRKTPRHLFVDEALAHRAYEDTALPIGHNQTISQPYMVAR
MSELLLAAGPLDKVMEIGTGSGYQTAVLAQLVERVFSVERIKGLQDRAKERLVELNLRNVVFRWGDGWEGWPALAPYNGI
IVTAVATDVPQALLDQLAPGGRLVIPVGSGEVQQLMLIIREENGFSRHVLGAVRFVPLLNGPIA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=231, Percent_Identity=35.0649350649351, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1789100, Length=211, Percent_Identity=53.0805687203791, Blast_Score=202, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI71983477, Length=217, Percent_Identity=35.0230414746544, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI193207222, Length=214, Percent_Identity=32.7102803738318, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17981723, Length=219, Percent_Identity=32.8767123287671, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 25051; Mature: 24920

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREQDDLLRRGIGMTSQRTRERLIQRLCEEGISNQRVLDVIRKTPRHLFVDEALAHRAY
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
EDTALPIGHNQTISQPYMVARMSELLLAAGPLDKVMEIGTGSGYQTAVLAQLVERVFSVE
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RIKGLQDRAKERLVELNLRNVVFRWGDGWEGWPALAPYNGIIVTAVATDVPQALLDQLAP
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCC
GGRLVIPVGSGEVQQLMLIIREENGFSRHVLGAVRFVPLLNGPIA
CCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SREQDDLLRRGIGMTSQRTRERLIQRLCEEGISNQRVLDVIRKTPRHLFVDEALAHRAY
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
EDTALPIGHNQTISQPYMVARMSELLLAAGPLDKVMEIGTGSGYQTAVLAQLVERVFSVE
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RIKGLQDRAKERLVELNLRNVVFRWGDGWEGWPALAPYNGIIVTAVATDVPQALLDQLAP
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCC
GGRLVIPVGSGEVQQLMLIIREENGFSRHVLGAVRFVPLLNGPIA
CCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]