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The map label for this gene is leuA [H]

Identifier: 66044505

GI number: 66044505

Start: 1413086

End: 1414756

Strand: Reverse

Name: leuA [H]

Synonym: Psyr_1257

Alternate gene names: 66044505

Gene position: 1414756-1413086 (Counterclockwise)

Preceding gene: 66044506

Following gene: 66044504

Centisome position: 23.22

GC content: 60.74

Gene sequence:

>1671_bases
ATGACCATGCTCAAAGACCCGTCGAAGAAGTACCGCGCGTTCCCGACCATTGATCTGCCTGACCGTACCTGGCCTTCGAA
GACCATCGACGCAGTGCCGATCTGGTGCAGCTCCGATCTGCGCGACGGCAACCAGTCGCTGATCGAACCGATGGACGCAG
CCAAGAAGCTGCGCTTCTGGAAAACCCTGGTCAGCGTCGGCGTCAAGGAAATCGAGGCATCCTTTCCATCGGCTTCGCAG
ACTGACTTCGACTTCGTGCGTACCCTGATCGAAGACGGTCACATCCCGGATGACACCACCATCCAGGTACTGACCCAGGC
TCGCGAAGACCTGATCGCCCGCACCTTCGAATCACTGCGCGGCGCCAAGAAAGCCATCGTTCACCTGTATAACGCGACCT
GCCCGTCGTTCCGCCGCATTGTCTTCAATCAGGACAAGGCCGGGGTGAAGGAGATCGCGGTCAACGCCGCCAAGCTGTTC
GTCAAATACGCAGCGCAGCAGCCGGAAACCCAGTGGACCTTCCAGTACTCGCCGGAGACCTTCAGTGCGACCGAACTGGA
ATTCGCCAAGGAAGTCTGTGATGCGGTGATCGAGGTGTGGAATCCCACGCCAGAGAACAAGGTGATCCTCAACCTCCCGG
CCACCGTGGAAGTGGCGACACCCAACATTTACGCCGACCAGATCGAATGGTTTGGCCGCAACATTACCCGCCGCGACAGC
GTGCTGATCAGCCTGCACACCCACAACGACCGTGGTACGGGCGTTGCCGCGACCGAGCTGGGCCTGATGGCCGGCGCGGA
TCGCGTCGAGGGCTGCCTGTTCGGTAACGGCGAGCGGACCGGTAACGTCGATCTGGTCACCGTGGCGCTGAACCTCTATA
CCCAGGGCATCAATCCCGGGCTGGACTTCTCGGACATCGACGGCGTGCGCAAGGTCGTCGAAGAGTGCAACCAGATCCCG
GTGCACCCACGTCATCCGTACGTTGGCGACCTGGTTCACACCGCGTTCTCCGGCTCGCACCAGGATGCGATCCGCAAGGG
TTTCACCCAGCAGAAGGAAGGCGAGTTGTGGGAAGTGCCTTACCTGCCGATCGACCCGGCCGACATCGGTCGCAGCTACG
AGGCGGTGATTCGCGTCAACAGCCAGTCAGGCAAAGGTGGCATCGCTTACCTGCTGGAACAGGAATATGGCATTTCCCTG
CCACGCCGCATGCAGATCGAGTTCAGCCAAGTGGTTCAGGGTGAGACCGATCGCCTGGGCCTGGAGATGACGGCCGAGCA
GATTCACTCGCTGTTGCGTCGTGAATACCTGCAAGCCAACGTTCCTTATGCGCTGATCAGCCATAAGTTGCAGGAAGAGA
ACGGCAACAGCTCGGTAGATGCCGAGGTGCACGTCGACGGCGAAACCCAGCACTGGCGTGGCAAGGGCAAGGGCGCCCTG
GAAGCACTGGTCGCCGGTCTGCCAGTGGCCGTGGAAATCATGGACTACAACGAGCATGCGATCGGTTCGGGTACGACCGC
CAAGGCGGCCGCCTACATTGAGCTGCGCGTGAATGGCGAACGCGCCGTGCATGGTGTGGGCATCGATGAAAACATCACCA
CGGCGAGCTTCCGCGCCCTGTTCAGCGCGCTTAATCGCTCACTCAGCCAGACTCAGGCCAAAGCTGCCTGA

Upstream 100 bases:

>100_bases
GCATGCCGTGCCCCGTCCCTGTGTTCGCAATTCCCTGTGCTATCTATCCAGAACGGCAGGCCGCCCAACCCCGGCCCCGT
GAATACAAAAGGATTTCCTG

Downstream 100 bases:

>100_bases
TTTGACAGCCGGATGGCGGACGCAGGTCCGCCATCCGCAGCGCCCGCTTCCCTCCCCCGCCTCACGCTGCACCTGCCTCT
TGCATTCCGCGCATGACCCT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MTMLKDPSKKYRAFPTIDLPDRTWPSKTIDAVPIWCSSDLRDGNQSLIEPMDAAKKLRFWKTLVSVGVKEIEASFPSASQ
TDFDFVRTLIEDGHIPDDTTIQVLTQAREDLIARTFESLRGAKKAIVHLYNATCPSFRRIVFNQDKAGVKEIAVNAAKLF
VKYAAQQPETQWTFQYSPETFSATELEFAKEVCDAVIEVWNPTPENKVILNLPATVEVATPNIYADQIEWFGRNITRRDS
VLISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNLYTQGINPGLDFSDIDGVRKVVEECNQIP
VHPRHPYVGDLVHTAFSGSHQDAIRKGFTQQKEGELWEVPYLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGISL
PRRMQIEFSQVVQGETDRLGLEMTAEQIHSLLRREYLQANVPYALISHKLQEENGNSSVDAEVHVDGETQHWRGKGKGAL
EALVAGLPVAVEIMDYNEHAIGSGTTAKAAAYIELRVNGERAVHGVGIDENITTASFRALFSALNRSLSQTQAKAA

Sequences:

>Translated_556_residues
MTMLKDPSKKYRAFPTIDLPDRTWPSKTIDAVPIWCSSDLRDGNQSLIEPMDAAKKLRFWKTLVSVGVKEIEASFPSASQ
TDFDFVRTLIEDGHIPDDTTIQVLTQAREDLIARTFESLRGAKKAIVHLYNATCPSFRRIVFNQDKAGVKEIAVNAAKLF
VKYAAQQPETQWTFQYSPETFSATELEFAKEVCDAVIEVWNPTPENKVILNLPATVEVATPNIYADQIEWFGRNITRRDS
VLISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNLYTQGINPGLDFSDIDGVRKVVEECNQIP
VHPRHPYVGDLVHTAFSGSHQDAIRKGFTQQKEGELWEVPYLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGISL
PRRMQIEFSQVVQGETDRLGLEMTAEQIHSLLRREYLQANVPYALISHKLQEENGNSSVDAEVHVDGETQHWRGKGKGAL
EALVAGLPVAVEIMDYNEHAIGSGTTAKAAAYIELRVNGERAVHGVGIDENITTASFRALFSALNRSLSQTQAKAA
>Mature_555_residues
TMLKDPSKKYRAFPTIDLPDRTWPSKTIDAVPIWCSSDLRDGNQSLIEPMDAAKKLRFWKTLVSVGVKEIEASFPSASQT
DFDFVRTLIEDGHIPDDTTIQVLTQAREDLIARTFESLRGAKKAIVHLYNATCPSFRRIVFNQDKAGVKEIAVNAAKLFV
KYAAQQPETQWTFQYSPETFSATELEFAKEVCDAVIEVWNPTPENKVILNLPATVEVATPNIYADQIEWFGRNITRRDSV
LISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNLYTQGINPGLDFSDIDGVRKVVEECNQIPV
HPRHPYVGDLVHTAFSGSHQDAIRKGFTQQKEGELWEVPYLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGISLP
RRMQIEFSQVVQGETDRLGLEMTAEQIHSLLRREYLQANVPYALISHKLQEENGNSSVDAEVHVDGETQHWRGKGKGALE
ALVAGLPVAVEIMDYNEHAIGSGTTAKAAAYIELRVNGERAVHGVGIDENITTASFRALFSALNRSLSQTQAKAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=537, Percent_Identity=24.0223463687151, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324225, Length=565, Percent_Identity=52.212389380531, Blast_Score=577, Evalue=1e-165,
Organism=Saccharomyces cerevisiae, GI6324682, Length=565, Percent_Identity=50.6194690265487, Blast_Score=574, Evalue=1e-164,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 61509; Mature: 61378

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLKDPSKKYRAFPTIDLPDRTWPSKTIDAVPIWCSSDLRDGNQSLIEPMDAAKKLRFW
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
KTLVSVGVKEIEASFPSASQTDFDFVRTLIEDGHIPDDTTIQVLTQAREDLIARTFESLR
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GAKKAIVHLYNATCPSFRRIVFNQDKAGVKEIAVNAAKLFVKYAAQQPETQWTFQYSPET
HHHHHHHHHHCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
FSATELEFAKEVCDAVIEVWNPTPENKVILNLPATVEVATPNIYADQIEWFGRNITRRDS
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCHHHHHHHCCCCCCCCC
VLISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNLYTQGINPG
EEEEEEECCCCCCCEEHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC
LDFSDIDGVRKVVEECNQIPVHPRHPYVGDLVHTAFSGSHQDAIRKGFTQQKEGELWEVP
CCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEECC
YLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGISLPRRMQIEFSQVVQGETDRLG
CCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEEHHHHHCCCCHHCC
LEMTAEQIHSLLRREYLQANVPYALISHKLQEENGNSSVDAEVHVDGETQHWRGKGKGAL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHH
EALVAGLPVAVEIMDYNEHAIGSGTTAKAAAYIELRVNGERAVHGVGIDENITTASFRAL
HHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEEECCCEEEEECCCCCCCHHHHHHHH
FSALNRSLSQTQAKAA
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TMLKDPSKKYRAFPTIDLPDRTWPSKTIDAVPIWCSSDLRDGNQSLIEPMDAAKKLRFW
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
KTLVSVGVKEIEASFPSASQTDFDFVRTLIEDGHIPDDTTIQVLTQAREDLIARTFESLR
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GAKKAIVHLYNATCPSFRRIVFNQDKAGVKEIAVNAAKLFVKYAAQQPETQWTFQYSPET
HHHHHHHHHHCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
FSATELEFAKEVCDAVIEVWNPTPENKVILNLPATVEVATPNIYADQIEWFGRNITRRDS
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCHHHHHHHCCCCCCCCC
VLISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNLYTQGINPG
EEEEEEECCCCCCCEEHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC
LDFSDIDGVRKVVEECNQIPVHPRHPYVGDLVHTAFSGSHQDAIRKGFTQQKEGELWEVP
CCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEECC
YLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGISLPRRMQIEFSQVVQGETDRLG
CCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEEHHHHHCCCCHHCC
LEMTAEQIHSLLRREYLQANVPYALISHKLQEENGNSSVDAEVHVDGETQHWRGKGKGAL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHH
EALVAGLPVAVEIMDYNEHAIGSGTTAKAAAYIELRVNGERAVHGVGIDENITTASFRAL
HHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEEECCCEEEEECCCCCCCHHHHHHHH
FSALNRSLSQTQAKAA
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA