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The map label for this gene is yafV [H]

Identifier: 66044502

GI number: 66044502

Start: 1410450

End: 1411241

Strand: Direct

Name: yafV [H]

Synonym: Psyr_1254

Alternate gene names: 66044502

Gene position: 1410450-1411241 (Clockwise)

Preceding gene: 66044501

Following gene: 66044508

Centisome position: 23.15

GC content: 60.48

Gene sequence:

>792_bases
ATGCGCGATCTAAGCGGGCTGCCGAACCTCAACCTCGCGCTGATCCAGACCACGCTGGCGTGGCATGATCGCGAGGCCAA
TCTCGAGCATTTCGAGCCATTACTGGATGAGGCGTGCGGCGCGGACCTGGTGATCCTGCCAGAGATGTTCACCACCGGAT
TCTCGATGGAGTCTGAGGCCCTGTCAGAACCGGAAGCCGGGCCTACATCAGTATGGCTATTGGCTCAGGCCAAGCGTATC
AACGCCGTAGTGACCGGCAGCGTCATGATCCGCGCTGCCGAGGGCAGTCATCGCAACCGCTTGCTGTGGGCGCGACCGGA
TGGTGCGCTGCTGCATTACGACAAGCGCCACCTGTTTCGCATGGCCGGAGAGCACGAGCATTACACGCCGGGCGATCGCC
AGGTCATGTTTGAACTCAACGGCTGGCGTGTACGGCCGCTGATCTGCTATGACCTGCGCTTTCCGGTCTGGAGCCGTGAT
GCTCAGGACACCGACCTGTTGCTGTACACCGCCAACTGGCCGGGTGCGCGGCGTCTGCACTGGAATCGCTTGCTGCCTGC
CAGAGCCATTGAAAACCTGTGCTATGTGGCGGCAGTCAACCGGGTCGGATCGGACGGCAAGGGCTTCGCTTACACTGGTG
ACAGTCAGGTTCTGGATTTCCAGGGCGAGAGCCTGTTGAGCGTGGGCGAAGCGGACGGAGTGTTCAAGGCGACGCTCAGC
GCGGCAGACTTGCATGCCTATCGCACACGGTTTCCAGCCGGGCTGGATGCTGATTCATTTAATATTGAATAA

Upstream 100 bases:

>100_bases
CGATTTCTGTGTTCTACCAGAGCCCGCCAGCAGGGCAGCGCCTGATCCGCCTGTGCTTTGCCAAACAGGAAGAGACGTTG
CGTCAGGCGGCGGAGAAACT

Downstream 100 bases:

>100_bases
AAGTACTTCAGAATGTTTCTGGTCGGCCGATAGCTTCTCCAGAATTCCTGGAGACCGCCATGACTACCATCAATACATCC
ACGCTGAACAGTTACTCAAG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MRDLSGLPNLNLALIQTTLAWHDREANLEHFEPLLDEACGADLVILPEMFTTGFSMESEALSEPEAGPTSVWLLAQAKRI
NAVVTGSVMIRAAEGSHRNRLLWARPDGALLHYDKRHLFRMAGEHEHYTPGDRQVMFELNGWRVRPLICYDLRFPVWSRD
AQDTDLLLYTANWPGARRLHWNRLLPARAIENLCYVAAVNRVGSDGKGFAYTGDSQVLDFQGESLLSVGEADGVFKATLS
AADLHAYRTRFPAGLDADSFNIE

Sequences:

>Translated_263_residues
MRDLSGLPNLNLALIQTTLAWHDREANLEHFEPLLDEACGADLVILPEMFTTGFSMESEALSEPEAGPTSVWLLAQAKRI
NAVVTGSVMIRAAEGSHRNRLLWARPDGALLHYDKRHLFRMAGEHEHYTPGDRQVMFELNGWRVRPLICYDLRFPVWSRD
AQDTDLLLYTANWPGARRLHWNRLLPARAIENLCYVAAVNRVGSDGKGFAYTGDSQVLDFQGESLLSVGEADGVFKATLS
AADLHAYRTRFPAGLDADSFNIE
>Mature_263_residues
MRDLSGLPNLNLALIQTTLAWHDREANLEHFEPLLDEACGADLVILPEMFTTGFSMESEALSEPEAGPTSVWLLAQAKRI
NAVVTGSVMIRAAEGSHRNRLLWARPDGALLHYDKRHLFRMAGEHEHYTPGDRQVMFELNGWRVRPLICYDLRFPVWSRD
AQDTDLLLYTANWPGARRLHWNRLLPARAIENLCYVAAVNRVGSDGKGFAYTGDSQVLDFQGESLLSVGEADGVFKATLS
AADLHAYRTRFPAGLDADSFNIE

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=264, Percent_Identity=27.6515151515151, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1786412, Length=257, Percent_Identity=50.1945525291829, Blast_Score=253, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6323383, Length=267, Percent_Identity=24.7191011235955, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29452; Mature: 29452

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50263 CN_HYDROLASE ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLSGLPNLNLALIQTTLAWHDREANLEHFEPLLDEACGADLVILPEMFTTGFSMESEA
CCCCCCCCCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHCCCCEEEECHHHHCCCCCCHHH
LSEPEAGPTSVWLLAQAKRINAVVTGSVMIRAAEGSHRNRLLWARPDGALLHYDKRHLFR
HCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCCCCCEEEECCCCCEEEECHHHHHH
MAGEHEHYTPGDRQVMFELNGWRVRPLICYDLRFPVWSRDAQDTDLLLYTANWPGARRLH
HCCCCCCCCCCCCEEEEEECCEEEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCEEE
WNRLLPARAIENLCYVAAVNRVGSDGKGFAYTGDSQVLDFQGESLLSVGEADGVFKATLS
HHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCEEEECCCCCEEEEHHH
AADLHAYRTRFPAGLDADSFNIE
HHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRDLSGLPNLNLALIQTTLAWHDREANLEHFEPLLDEACGADLVILPEMFTTGFSMESEA
CCCCCCCCCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHCCCCEEEECHHHHCCCCCCHHH
LSEPEAGPTSVWLLAQAKRINAVVTGSVMIRAAEGSHRNRLLWARPDGALLHYDKRHLFR
HCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCCCCCEEEECCCCCEEEECHHHHHH
MAGEHEHYTPGDRQVMFELNGWRVRPLICYDLRFPVWSRDAQDTDLLLYTANWPGARRLH
HCCCCCCCCCCCCEEEEEECCEEEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCEEE
WNRLLPARAIENLCYVAAVNRVGSDGKGFAYTGDSQVLDFQGESLLSVGEADGVFKATLS
HHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCEEEECCCCCEEEEHHH
AADLHAYRTRFPAGLDADSFNIE
HHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]