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The map label for this gene is rlmN [H]

Identifier: 66044493

GI number: 66044493

Start: 1400404

End: 1401552

Strand: Direct

Name: rlmN [H]

Synonym: Psyr_1245

Alternate gene names: 66044493

Gene position: 1400404-1401552 (Clockwise)

Preceding gene: 66044492

Following gene: 66044494

Centisome position: 22.98

GC content: 57.79

Gene sequence:

>1149_bases
ATGATTGCATCGACTGGTAAAACCAACCTGCTGGGGCTGACTCAACAGGAAATGGAGAAATTCTTCGACTCTATCGGGGA
GAAGCGCTTCCGTGCCGGTCAGGTCATGAAGTGGATTCACCACTTCGGCGTCGACGATTTCGACGCGATGACGAACGTCA
GCAAGGCCCTGCGCGAAAAGCTCAAGGCCTGTGCTGAGGTTCGCGGTCCTGAAGTGGTCAGCGAGGACATCTCCAGCGAC
GGCACCCGTAAATGGGTGGTGCGCGTAGAGTCCGGCAGCTGTGTCGAGACCGTCTATATTCCGCAGGGCAAGCGCGGCAC
CTTGTGTGTTTCGTCCCAGGCGGGCTGTGCCCTGGATTGCAGTTTCTGCTCCACGGGCAAGCAAGGCTTCAACAGCAACC
TCACCGCCGCCGAAGTCATCGGTCAGGTGTGGATTGCCAACAAATCCTTTGGCAGCGTCCCGGCGACCGTCGACCGTGCC
ATCACCAACGTGGTGATGATGGGCATGGGTGAGCCGCTGCTGAACTTCGACAATGTCATTGCCGCCATGCATCTGATGAT
GGATGACCTGGGGTATGGCATTTCCAAGCGCCGGGTGACCTTGTCTACTTCCGGTGTGGTACCGATGATCGATGAGCTTT
CCAAGCACATCGACGTGTCCCTGGCCTTGTCGCTGCACGCACCGAACGATGCGCTGCGCAACCAGCTGGTGCCGATCAAC
AAGAAATACCCGTTGAAAATGCTTCTCGAGTCCTGCCGTCGCTACATGTCCAGTCTGGGCGAAAAGCGTGTGCTCACCAT
CGAGTACACCATGCTCAAGGACATCAACGACAAGGTAGAGCACGCGGTCGAGATGATCGAACTGCTCAAGGACACGCCTT
GCAAGATCAACCTGATCCCGTTCAACCCATTTCCGCACTCCGGTTACGAGCGTCCCAGCAATAACGCGATACGTCGTTTT
CAGGACCTGTTGCATCAGGCCGGTTATAACGTCACTGTGCGCACCACGCGTGGTGAAGACATCGATGCTGCATGCGGCCA
GTTGGTCGGGCAGGTGATGGACCGCACACGCCGTAGCGAACGCTATATAGCCGTGCGTGAGTTGAGCGCCGAGGCCGATG
CGGCCCCTGTCGCGGTGACTCGAACCTGA

Upstream 100 bases:

>100_bases
TTCCGAAGCAGCCGCCGCTCGCGAAATCTCGTACTTCTTCGCAGCTACCGAAGTAACCGCTCGCTAAGTCATTTTTGACT
GACGAGTGAAGGGTGAATTC

Downstream 100 bases:

>100_bases
CTGCGAACCTGACCGAGAGAGACCCCATGCCTGTGCGTGCCTCGCTGCTGCTCTGTGTTGTCGTCTTGCTGACAGCGTGC
GTGTCCACCGGCAACGTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MIASTGKTNLLGLTQQEMEKFFDSIGEKRFRAGQVMKWIHHFGVDDFDAMTNVSKALREKLKACAEVRGPEVVSEDISSD
GTRKWVVRVESGSCVETVYIPQGKRGTLCVSSQAGCALDCSFCSTGKQGFNSNLTAAEVIGQVWIANKSFGSVPATVDRA
ITNVVMMGMGEPLLNFDNVIAAMHLMMDDLGYGISKRRVTLSTSGVVPMIDELSKHIDVSLALSLHAPNDALRNQLVPIN
KKYPLKMLLESCRRYMSSLGEKRVLTIEYTMLKDINDKVEHAVEMIELLKDTPCKINLIPFNPFPHSGYERPSNNAIRRF
QDLLHQAGYNVTVRTTRGEDIDAACGQLVGQVMDRTRRSERYIAVRELSAEADAAPVAVTRT

Sequences:

>Translated_382_residues
MIASTGKTNLLGLTQQEMEKFFDSIGEKRFRAGQVMKWIHHFGVDDFDAMTNVSKALREKLKACAEVRGPEVVSEDISSD
GTRKWVVRVESGSCVETVYIPQGKRGTLCVSSQAGCALDCSFCSTGKQGFNSNLTAAEVIGQVWIANKSFGSVPATVDRA
ITNVVMMGMGEPLLNFDNVIAAMHLMMDDLGYGISKRRVTLSTSGVVPMIDELSKHIDVSLALSLHAPNDALRNQLVPIN
KKYPLKMLLESCRRYMSSLGEKRVLTIEYTMLKDINDKVEHAVEMIELLKDTPCKINLIPFNPFPHSGYERPSNNAIRRF
QDLLHQAGYNVTVRTTRGEDIDAACGQLVGQVMDRTRRSERYIAVRELSAEADAAPVAVTRT
>Mature_382_residues
MIASTGKTNLLGLTQQEMEKFFDSIGEKRFRAGQVMKWIHHFGVDDFDAMTNVSKALREKLKACAEVRGPEVVSEDISSD
GTRKWVVRVESGSCVETVYIPQGKRGTLCVSSQAGCALDCSFCSTGKQGFNSNLTAAEVIGQVWIANKSFGSVPATVDRA
ITNVVMMGMGEPLLNFDNVIAAMHLMMDDLGYGISKRRVTLSTSGVVPMIDELSKHIDVSLALSLHAPNDALRNQLVPIN
KKYPLKMLLESCRRYMSSLGEKRVLTIEYTMLKDINDKVEHAVEMIELLKDTPCKINLIPFNPFPHSGYERPSNNAIRRF
QDLLHQAGYNVTVRTTRGEDIDAACGQLVGQVMDRTRRSERYIAVRELSAEADAAPVAVTRT

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=358, Percent_Identity=60.3351955307263, Blast_Score=449, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 42283; Mature: 42283

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIASTGKTNLLGLTQQEMEKFFDSIGEKRFRAGQVMKWIHHFGVDDFDAMTNVSKALREK
CCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LKACAEVRGPEVVSEDISSDGTRKWVVRVESGSCVETVYIPQGKRGTLCVSSQAGCALDC
HHHHHHCCCCHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCEEEC
SFCSTGKQGFNSNLTAAEVIGQVWIANKSFGSVPATVDRAITNVVMMGMGEPLLNFDNVI
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
AAMHLMMDDLGYGISKRRVTLSTSGVVPMIDELSKHIDVSLALSLHAPNDALRNQLVPIN
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEEECCCHHHHCCCCCCC
KKYPLKMLLESCRRYMSSLGEKRVLTIEYTMLKDINDKVEHAVEMIELLKDTPCKINLIP
CCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
FNPFPHSGYERPSNNAIRRFQDLLHQAGYNVTVRTTRGEDIDAACGQLVGQVMDRTRRSE
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RYIAVRELSAEADAAPVAVTRT
HEEEEEHHCCCCCCCCEEEECC
>Mature Secondary Structure
MIASTGKTNLLGLTQQEMEKFFDSIGEKRFRAGQVMKWIHHFGVDDFDAMTNVSKALREK
CCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LKACAEVRGPEVVSEDISSDGTRKWVVRVESGSCVETVYIPQGKRGTLCVSSQAGCALDC
HHHHHHCCCCHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCEEEC
SFCSTGKQGFNSNLTAAEVIGQVWIANKSFGSVPATVDRAITNVVMMGMGEPLLNFDNVI
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
AAMHLMMDDLGYGISKRRVTLSTSGVVPMIDELSKHIDVSLALSLHAPNDALRNQLVPIN
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEEECCCHHHHCCCCCCC
KKYPLKMLLESCRRYMSSLGEKRVLTIEYTMLKDINDKVEHAVEMIELLKDTPCKINLIP
CCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
FNPFPHSGYERPSNNAIRRFQDLLHQAGYNVTVRTTRGEDIDAACGQLVGQVMDRTRRSE
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RYIAVRELSAEADAAPVAVTRT
HEEEEEHHCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA