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The map label for this gene is iscA [H]

Identifier: 66044487

GI number: 66044487

Start: 1396526

End: 1396849

Strand: Direct

Name: iscA [H]

Synonym: Psyr_1239

Alternate gene names: 66044487

Gene position: 1396526-1396849 (Clockwise)

Preceding gene: 66044486

Following gene: 161486736

Centisome position: 22.92

GC content: 57.72

Gene sequence:

>324_bases
ATGGCTATCAGCATGACAGAAGCTGCCGCTAACCACGTACGACGTTCCCTCGAGGGGCGCGGCAAGGGTGACGGCGTTCG
TCTGGGTGTTCGCACCACAGGCTGTTCAGGTCTTGCCTATGTGCTCGAGTTCGTCGATGAGGCGGCCAGCGAAGACACCG
TGTTCGAAATGCACGGCGTCAAGGTGATCATTGACCCGAAAAGTCTGGTCTATCTGGATGGCACCGAGCTCGACTTCGTG
CGCGAAGGGTTGAACGAAGGCTTCAAGTTCAACAATCCCAACTCGCGCGGTGAGTGCGGCTGTGGCGAAAGCTTCAACGT
CTGA

Upstream 100 bases:

>100_bases
ACTACAAGCAGAAGAAAGGTCTGCTGTAAGCAGGCTTTTCAAGAGAAGCTTGCAACAGAGTCAACTGCAACAGGCTAATG
CAACAGGCTAAGGAGTTCCG

Downstream 100 bases:

>100_bases
GGCTTTTATTGTGGGTACTCCCTGTCACTTCGCCCTGTTTGACCTCAAGCCCGAGTTTCAGCTTGATCTTGACCAACTGG
CCACGCGCTACCGTGAGCTT

Product: HesB/YadR/YfhF:Iron-sulphur cluster assembly protein IscA

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MAISMTEAAANHVRRSLEGRGKGDGVRLGVRTTGCSGLAYVLEFVDEAASEDTVFEMHGVKVIIDPKSLVYLDGTELDFV
REGLNEGFKFNNPNSRGECGCGESFNV

Sequences:

>Translated_107_residues
MAISMTEAAANHVRRSLEGRGKGDGVRLGVRTTGCSGLAYVLEFVDEAASEDTVFEMHGVKVIIDPKSLVYLDGTELDFV
REGLNEGFKFNNPNSRGECGCGESFNV
>Mature_106_residues
AISMTEAAANHVRRSLEGRGKGDGVRLGVRTTGCSGLAYVLEFVDEAASEDTVFEMHGVKVIIDPKSLVYLDGTELDFVR
EGLNEGFKFNNPNSRGECGCGESFNV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=43.3962264150943, Blast_Score=102, Evalue=7e-23,
Organism=Homo sapiens, GI160420328, Length=96, Percent_Identity=35.4166666666667, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=67.2897196261682, Blast_Score=151, Evalue=7e-39,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=49.5238095238095, Blast_Score=115, Evalue=8e-28,
Organism=Escherichia coli, GI1786351, Length=111, Percent_Identity=40.5405405405405, Blast_Score=72, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=39.622641509434, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=39.622641509434, Blast_Score=96, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=41.9047619047619, Blast_Score=95, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=42.4528301886792, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11547; Mature: 11416

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISMTEAAANHVRRSLEGRGKGDGVRLGVRTTGCSGLAYVLEFVDEAASEDTVFEMHGV
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCE
KVIIDPKSLVYLDGTELDFVREGLNEGFKFNNPNSRGECGCGESFNV
EEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AISMTEAAANHVRRSLEGRGKGDGVRLGVRTTGCSGLAYVLEFVDEAASEDTVFEMHGV
CCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCE
KVIIDPKSLVYLDGTELDFVREGLNEGFKFNNPNSRGECGCGESFNV
EEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA