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The map label for this gene is mltD [H]

Identifier: 66044440

GI number: 66044440

Start: 1348699

End: 1350222

Strand: Direct

Name: mltD [H]

Synonym: Psyr_1189

Alternate gene names: 66044440

Gene position: 1348699-1350222 (Clockwise)

Preceding gene: 66044438

Following gene: 66044441

Centisome position: 22.13

GC content: 63.45

Gene sequence:

>1524_bases
ATGGCCGCCGTTGCCCTCCACGTTTCGTCTCCTCGTTTGCTGGCTCGTTCGGTACAGATCGCCGTTCTGGCCCTGGGGGC
GCTGTGTGTGGGCTGTCAGTCAGTCGATGACTCACCGTCGCGCCTGGACCGCTTGCCGCGACTGGTCGCCGGGCTGGGGT
ATCGCTCGGCCGACGCGCAGGCCGACCCCCTGGCTGCAACGACTGTTGCCGATTTGCCCGTCTACAACGGCGAGGACGTT
TGGCAACGGGTGGCGCAGCGATGCCGGCTGGTGGACGGGCAGGGCGTCAATGAACGTATCGCTCGCCAGCGTGACTGGTT
GCTGAACAATCGCGGTTTTCTGACCGGGGCCAGCAAGCGCGCCACGCCTTACCTGCATTTCATTGTCGAACGTCTCGATG
AACGCAACATGCCACTGGAACTGGCGCTGCTGCCCATGATCGAGAGCTCCTACAATCCCTTGGCCAACTCCCCGGCGGCG
GCAGCGGGCCTCTGGCAGTTCATCCCGTCTACCGGTCGCCAGTTCAACTTGAACCAGAGCGCTACCTACGACGCCCGCCG
CGATGTTGTCGCGTCAAGCAAGGCGGCGATGGACTATCTGACTCGCCTGCATGATCAGTTCAATAACGACTGGCTGTTGG
CACTGGCGGCCTATAACGCAGGCGAGGGCACGGTTGCACGAGCGATCGACGCCAACCGTCGACGTGGCATGCCTGTCGAC
TACTGGCATTTGAATTTACCCCGGGAAACCCAGGACTACGTTCCGCGCCTGCTGGCACTTTCGCTGGTGGTGCGCAATCC
AGGCGCCTACGGCGTGCAACTGACGCCGGTCGCCAACATGCCGTATTTCGATGTGGTTGAGCTCAACCACACGGTAGACC
TGACTCAGCTCGCCGCCACCACCGGGGTAGACGAGGCACAGTTGTTGCGCCTCAACCCGGCATTCCTGCGAAAAAAGACC
CCGGATGGCCCGGGACGTTTGCTGATTCCCAAGACCCACACGCAGGTGTTGACGGCAGGTATCGAGCGGATCACCGGAGA
GTCGCCGACAACCGCTGTCATCCCGGCAAGTCCCGGAAAGCGTGTGATCGAGCGCGAGCCATCGTCGCAAGTGGCCGAGC
GCGCTCCGACGCCTGAGCCGTTGCAGGCAGCAGGGCGCGAGCAGACGGCAGGCCTGATGCAGGTGCTTGAGCGCGAGCCA
GCACCGGAATCGGTGCAGCCGGTCAGGCGTGAACCGCAACCGAAAGTCGTCCAGGTTGCCAGGCAGGAGCCCTTGCCCGG
CCCCGCGCAACCCGTTCAGGCTGCCGAACAGGTAACTGTGTCCGACCCCATCCAGCCTGCGCGTCAGGCGTCGTCGCAGG
CGACAAACGAGCGTTCGCTACTGGACCGCCGGATACAAAAGAGGCTGTACATTGATGACCGTTCGTCGCCACGCAAGCGC
GACGAGATCGCGTATCGCGATGAGCCGCGTGAGCTGCCGAACGGTCCTCGTGTCGTGGTGTACGCCAGCGATCCACGCCA
ATAA

Upstream 100 bases:

>100_bases
AAACGTTTCAGGCTTGTGCGTACGCAAGCGAAATACGCCTTGCAGGGAACTGGAAAGGCGGGCGTGCCACGTAGGGGGAC
ACGTTCAGGACATTCCGCAC

Downstream 100 bases:

>100_bases
CCTCTCGATTTTTTTCTCCTGAGCGCTTAAAGCACCTGAAAATTGCCCTTTTTTAGTGCTTTTTCGCGTTCCCAGCGCGT
GCCCGAAACCTTCGAATAAA

Product: SLT:MLTD_N

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MAAVALHVSSPRLLARSVQIAVLALGALCVGCQSVDDSPSRLDRLPRLVAGLGYRSADAQADPLAATTVADLPVYNGEDV
WQRVAQRCRLVDGQGVNERIARQRDWLLNNRGFLTGASKRATPYLHFIVERLDERNMPLELALLPMIESSYNPLANSPAA
AAGLWQFIPSTGRQFNLNQSATYDARRDVVASSKAAMDYLTRLHDQFNNDWLLALAAYNAGEGTVARAIDANRRRGMPVD
YWHLNLPRETQDYVPRLLALSLVVRNPGAYGVQLTPVANMPYFDVVELNHTVDLTQLAATTGVDEAQLLRLNPAFLRKKT
PDGPGRLLIPKTHTQVLTAGIERITGESPTTAVIPASPGKRVIEREPSSQVAERAPTPEPLQAAGREQTAGLMQVLEREP
APESVQPVRREPQPKVVQVARQEPLPGPAQPVQAAEQVTVSDPIQPARQASSQATNERSLLDRRIQKRLYIDDRSSPRKR
DEIAYRDEPRELPNGPRVVVYASDPRQ

Sequences:

>Translated_507_residues
MAAVALHVSSPRLLARSVQIAVLALGALCVGCQSVDDSPSRLDRLPRLVAGLGYRSADAQADPLAATTVADLPVYNGEDV
WQRVAQRCRLVDGQGVNERIARQRDWLLNNRGFLTGASKRATPYLHFIVERLDERNMPLELALLPMIESSYNPLANSPAA
AAGLWQFIPSTGRQFNLNQSATYDARRDVVASSKAAMDYLTRLHDQFNNDWLLALAAYNAGEGTVARAIDANRRRGMPVD
YWHLNLPRETQDYVPRLLALSLVVRNPGAYGVQLTPVANMPYFDVVELNHTVDLTQLAATTGVDEAQLLRLNPAFLRKKT
PDGPGRLLIPKTHTQVLTAGIERITGESPTTAVIPASPGKRVIEREPSSQVAERAPTPEPLQAAGREQTAGLMQVLEREP
APESVQPVRREPQPKVVQVARQEPLPGPAQPVQAAEQVTVSDPIQPARQASSQATNERSLLDRRIQKRLYIDDRSSPRKR
DEIAYRDEPRELPNGPRVVVYASDPRQ
>Mature_506_residues
AAVALHVSSPRLLARSVQIAVLALGALCVGCQSVDDSPSRLDRLPRLVAGLGYRSADAQADPLAATTVADLPVYNGEDVW
QRVAQRCRLVDGQGVNERIARQRDWLLNNRGFLTGASKRATPYLHFIVERLDERNMPLELALLPMIESSYNPLANSPAAA
AGLWQFIPSTGRQFNLNQSATYDARRDVVASSKAAMDYLTRLHDQFNNDWLLALAAYNAGEGTVARAIDANRRRGMPVDY
WHLNLPRETQDYVPRLLALSLVVRNPGAYGVQLTPVANMPYFDVVELNHTVDLTQLAATTGVDEAQLLRLNPAFLRKKTP
DGPGRLLIPKTHTQVLTAGIERITGESPTTAVIPASPGKRVIEREPSSQVAERAPTPEPLQAAGREQTAGLMQVLEREPA
PESVQPVRREPQPKVVQVARQEPLPGPAQPVQAAEQVTVSDPIQPARQASSQATNERSLLDRRIQKRLYIDDRSSPRKRD
EIAYRDEPRELPNGPRVVVYASDPRQ

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=324, Percent_Identity=37.962962962963, Blast_Score=221, Evalue=8e-59,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 55882; Mature: 55751

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVALHVSSPRLLARSVQIAVLALGALCVGCQSVDDSPSRLDRLPRLVAGLGYRSADAQ
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
ADPLAATTVADLPVYNGEDVWQRVAQRCRLVDGQGVNERIARQRDWLLNNRGFLTGASKR
CCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCC
ATPYLHFIVERLDERNMPLELALLPMIESSYNPLANSPAAAAGLWQFIPSTGRQFNLNQS
CCHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCC
ATYDARRDVVASSKAAMDYLTRLHDQFNNDWLLALAAYNAGEGTVARAIDANRRRGMPVD
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEHHHCCCCCCCCCCC
YWHLNLPRETQDYVPRLLALSLVVRNPGAYGVQLTPVANMPYFDVVELNHTVDLTQLAAT
EEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHH
TGVDEAQLLRLNPAFLRKKTPDGPGRLLIPKTHTQVLTAGIERITGESPTTAVIPASPGK
CCCCCHHEEECCHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCC
RVIEREPSSQVAERAPTPEPLQAAGREQTAGLMQVLEREPAPESVQPVRREPQPKVVQVA
HHHHCCCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHH
RQEPLPGPAQPVQAAEQVTVSDPIQPARQASSQATNERSLLDRRIQKRLYIDDRSSPRKR
HHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHEEECCCCCCCCH
DEIAYRDEPRELPNGPRVVVYASDPRQ
HCCCCCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
AAVALHVSSPRLLARSVQIAVLALGALCVGCQSVDDSPSRLDRLPRLVAGLGYRSADAQ
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
ADPLAATTVADLPVYNGEDVWQRVAQRCRLVDGQGVNERIARQRDWLLNNRGFLTGASKR
CCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCC
ATPYLHFIVERLDERNMPLELALLPMIESSYNPLANSPAAAAGLWQFIPSTGRQFNLNQS
CCHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCC
ATYDARRDVVASSKAAMDYLTRLHDQFNNDWLLALAAYNAGEGTVARAIDANRRRGMPVD
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEHHHCCCCCCCCCCC
YWHLNLPRETQDYVPRLLALSLVVRNPGAYGVQLTPVANMPYFDVVELNHTVDLTQLAAT
EEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHH
TGVDEAQLLRLNPAFLRKKTPDGPGRLLIPKTHTQVLTAGIERITGESPTTAVIPASPGK
CCCCCHHEEECCHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCC
RVIEREPSSQVAERAPTPEPLQAAGREQTAGLMQVLEREPAPESVQPVRREPQPKVVQVA
HHHHCCCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHH
RQEPLPGPAQPVQAAEQVTVSDPIQPARQASSQATNERSLLDRRIQKRLYIDDRSSPRKR
HHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHEEECCCCCCCCH
DEIAYRDEPRELPNGPRVVVYASDPRQ
HCCCCCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]