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The map label for this gene is rutB [H]

Identifier: 66044414

GI number: 66044414

Start: 1316740

End: 1317417

Strand: Reverse

Name: rutB [H]

Synonym: Psyr_1163

Alternate gene names: 66044414

Gene position: 1317417-1316740 (Counterclockwise)

Preceding gene: 66044415

Following gene: 66044413

Centisome position: 21.62

GC content: 63.13

Gene sequence:

>678_bases
ATGAACAAGGTCAATGCGCGCCCTGACCGCTTCGCCTTCGACACGTCCCGTACCGCTGTGGTGATCATCGACATGCAGCT
CGATTTCCTCGAACCCGGCGGCTTTGGTGCCGCACTGGGCAACGACGTCGCACCGTTGCAGGCCATCGTACCGTCCGTGC
AACGCCTGCTGACGCTGGCGCGCGATGAAGGCATGACGGTGATCCATACCCGCGAATCACACCGCCCGGACCTTGCCGAC
TGCCCGCAAGCCAAACGTGACCATGGCTCGCCGGGCCTGCGCATCGGCGATCCCGGCCCCATGGGGCGCATCCTGATTCG
CGGCGAACCCGGCAATCAGATTATCGACGCCCTGGCACCACTCGCTGACGAGTGGGTCATCGACAAACCCGGCAAAGGCA
TGTTCTTCGCCACCGACCTGCAGCAGCGGCTGAGCCAAGCCGGGATCACCCATTTGATCTTTGCCGGCGTCACCACCGAA
GTCTGCGTGCAGACCAGCATGCGCGAAGCCAATGACCGGGGTTATCGCTGCCTGCTGATCGAGGACGCGACCGAAAGCTA
CTTCCCTGCGTTCAAAAAGGCCACGCTGGAGATGATCACCGCACAAGGCGGGATCGTCGGCCGGGTTGCCTCGTTGACGG
ACCTGGAGCAGGCGCTGCAGACAAGGAGCACACACTGA

Upstream 100 bases:

>100_bases
ACGACGGCGAACTGGTGTTTGAAACCCGTGCAGCGGACGCCGACCGTACCGAGCTGGGCCGACACATGGCCGGGGCCGAG
CATCTCGCGGGGCAATCGGC

Downstream 100 bases:

>100_bases
TGGACATCAACCTCCCCCACGTGGTCGCTGAAGTGACCCACGCCTTTCACGACTACGAACGCGCCCTGCTGGCCAATGAA
CTGAGCACGCTGGATGCGTA

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MNKVNARPDRFAFDTSRTAVVIIDMQLDFLEPGGFGAALGNDVAPLQAIVPSVQRLLTLARDEGMTVIHTRESHRPDLAD
CPQAKRDHGSPGLRIGDPGPMGRILIRGEPGNQIIDALAPLADEWVIDKPGKGMFFATDLQQRLSQAGITHLIFAGVTTE
VCVQTSMREANDRGYRCLLIEDATESYFPAFKKATLEMITAQGGIVGRVASLTDLEQALQTRSTH

Sequences:

>Translated_225_residues
MNKVNARPDRFAFDTSRTAVVIIDMQLDFLEPGGFGAALGNDVAPLQAIVPSVQRLLTLARDEGMTVIHTRESHRPDLAD
CPQAKRDHGSPGLRIGDPGPMGRILIRGEPGNQIIDALAPLADEWVIDKPGKGMFFATDLQQRLSQAGITHLIFAGVTTE
VCVQTSMREANDRGYRCLLIEDATESYFPAFKKATLEMITAQGGIVGRVASLTDLEQALQTRSTH
>Mature_225_residues
MNKVNARPDRFAFDTSRTAVVIIDMQLDFLEPGGFGAALGNDVAPLQAIVPSVQRLLTLARDEGMTVIHTRESHRPDLAD
CPQAKRDHGSPGLRIGDPGPMGRILIRGEPGNQIIDALAPLADEWVIDKPGKGMFFATDLQQRLSQAGITHLIFAGVTTE
VCVQTSMREANDRGYRCLLIEDATESYFPAFKKATLEMITAQGGIVGRVASLTDLEQALQTRSTH

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=219, Percent_Identity=31.5068493150685, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 24480; Mature: 24480

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVNARPDRFAFDTSRTAVVIIDMQLDFLEPGGFGAALGNDVAPLQAIVPSVQRLLTLA
CCCCCCCCCCEEECCCCEEEEEEEEEEEEECCCCCCHHCCCCCHHHHHHHHHHHHHHHHH
RDEGMTVIHTRESHRPDLADCPQAKRDHGSPGLRIGDPGPMGRILIRGEPGNQIIDALAP
HCCCCEEEEECCCCCCCCCCCCCCHHCCCCCCCEECCCCCCCEEEEECCCCHHHHHHHHH
LADEWVIDKPGKGMFFATDLQQRLSQAGITHLIFAGVTTEVCVQTSMREANDRGYRCLLI
HHCCCEEECCCCCCEEHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEE
EDATESYFPAFKKATLEMITAQGGIVGRVASLTDLEQALQTRSTH
ECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKVNARPDRFAFDTSRTAVVIIDMQLDFLEPGGFGAALGNDVAPLQAIVPSVQRLLTLA
CCCCCCCCCCEEECCCCEEEEEEEEEEEEECCCCCCHHCCCCCHHHHHHHHHHHHHHHHH
RDEGMTVIHTRESHRPDLADCPQAKRDHGSPGLRIGDPGPMGRILIRGEPGNQIIDALAP
HCCCCEEEEECCCCCCCCCCCCCCHHCCCCCCCEECCCCCCCEEEEECCCCHHHHHHHHH
LADEWVIDKPGKGMFFATDLQQRLSQAGITHLIFAGVTTEVCVQTSMREANDRGYRCLLI
HHCCCEEECCCCCCEEHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEE
EDATESYFPAFKKATLEMITAQGGIVGRVASLTDLEQALQTRSTH
ECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA