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The map label for this gene is ecpD [H]

Identifier: 66044385

GI number: 66044385

Start: 1286361

End: 1287146

Strand: Direct

Name: ecpD [H]

Synonym: Psyr_1134

Alternate gene names: 66044385

Gene position: 1286361-1287146 (Clockwise)

Preceding gene: 66044384

Following gene: 66044386

Centisome position: 21.11

GC content: 64.12

Gene sequence:

>786_bases
ATGAGCGCTTTCGCTGGCTCATGCAAGGCGCTGTTGACGGCTGTGCTGTTGACGAGCGGCCTGGCGGCGCACGGGGCTGT
TTCGCTGAGCGGAACGCGGCTAATTTTCGACGGGCAGTATCGCGAGGTCACGCTCGAGGTGCGCAATCGCGGCAAGTCCG
AGGCACTTTTACAGGCTTGGCTCAGTGACGCAGCGGATGACGACGACGCCCCGGGTGAGCTTCGCAAGGCGCTGCCATTC
GTGGTCACGCCACCGCTTTCACGCTTGGCGGTGGGCGGCAGGCAGGCCTTGCGCGTGCTCTATCAGGGCACAGGCATGCC
GCAGAAACACGAGTCGCTGCTGCACCTGTATGTGCTTGAGATACCCCGACGCCAGGACGGTGCGGGGCAGTTGAACATCG
CCGTGCGCCAACGCATCAACGTGTTCTACCGGCCCCCCGGCCTTGACGGTGATCCGGCCGACACTCCCGCGCGGCTGCTG
TGGACACTGACTCATGACCCGTCTCACGCCTTGTTGCTGACGGTCAGTAACCCGACGCCCTATCACGCTGCCCTGCAAGC
GCTGCGTATCGACGGGGTGCAGCTTGGCGATCATCTGTTGCTGGCGCCGGGCGCACAGGTCGCGATGGTTGTGCCGGCGA
GTGTCGAGCGCTCTGCCACACATCGTTTTTCCTACCAGGCGCTGACCGACTACGGCGGCCGACGGACCCATTGCGCACAG
CTCACCGGGCAGACGGCCACTACCGCCAGGCTGCCGAAAAACAATTCCATTCAGGACGAATGCTGA

Upstream 100 bases:

>100_bases
GCCTGTGGGTGCGCTGGTCAGATCAACGTTGCCAGGCGACATTCTCGTTACCGCCCAGAGACCCGTCGCGAGCCTACGAG
CGGATCAGGGTGGTATGCCG

Downstream 100 bases:

>100_bases
TATGAAACATCTCTCTGCCAGAGGCTGGCTGCGATCCCTTGCCGTGTTGCTGTTACTGCATCCGGCTTCCACCTTCGCGC
TGATCTGTACCACTCAAGGG

Product: pili assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSAFAGSCKALLTAVLLTSGLAAHGAVSLSGTRLIFDGQYREVTLEVRNRGKSEALLQAWLSDAADDDDAPGELRKALPF
VVTPPLSRLAVGGRQALRVLYQGTGMPQKHESLLHLYVLEIPRRQDGAGQLNIAVRQRINVFYRPPGLDGDPADTPARLL
WTLTHDPSHALLLTVSNPTPYHAALQALRIDGVQLGDHLLLAPGAQVAMVVPASVERSATHRFSYQALTDYGGRRTHCAQ
LTGQTATTARLPKNNSIQDEC

Sequences:

>Translated_261_residues
MSAFAGSCKALLTAVLLTSGLAAHGAVSLSGTRLIFDGQYREVTLEVRNRGKSEALLQAWLSDAADDDDAPGELRKALPF
VVTPPLSRLAVGGRQALRVLYQGTGMPQKHESLLHLYVLEIPRRQDGAGQLNIAVRQRINVFYRPPGLDGDPADTPARLL
WTLTHDPSHALLLTVSNPTPYHAALQALRIDGVQLGDHLLLAPGAQVAMVVPASVERSATHRFSYQALTDYGGRRTHCAQ
LTGQTATTARLPKNNSIQDEC
>Mature_260_residues
SAFAGSCKALLTAVLLTSGLAAHGAVSLSGTRLIFDGQYREVTLEVRNRGKSEALLQAWLSDAADDDDAPGELRKALPFV
VTPPLSRLAVGGRQALRVLYQGTGMPQKHESLLHLYVLEIPRRQDGAGQLNIAVRQRINVFYRPPGLDGDPADTPARLLW
TLTHDPSHALLLTVSNPTPYHAALQALRIDGVQLGDHLLLAPGAQVAMVVPASVERSATHRFSYQALTDYGGRRTHCAQL
TGQTATTARLPKNNSIQDEC

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=224, Percent_Identity=33.0357142857143, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789532, Length=220, Percent_Identity=31.8181818181818, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1787171, Length=230, Percent_Identity=30, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788428, Length=144, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786743, Length=228, Percent_Identity=24.5614035087719, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1790771, Length=228, Percent_Identity=27.6315789473684, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788677, Length=222, Percent_Identity=26.1261261261261, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786936, Length=246, Percent_Identity=27.6422764227642, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082203, Length=239, Percent_Identity=25.1046025104602, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87081806, Length=128, Percent_Identity=28.90625, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 28153; Mature: 28022

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFAGSCKALLTAVLLTSGLAAHGAVSLSGTRLIFDGQYREVTLEVRNRGKSEALLQAW
CCCCCHHHHHHHHHHHHHCCHHHCCEEEECCCEEEECCCCEEEEEEECCCCHHHHHHHHH
LSDAADDDDAPGELRKALPFVVTPPLSRLAVGGRQALRVLYQGTGMPQKHESLLHLYVLE
HHHCCCCCCCCHHHHHCCCEEECCCHHHHHHCHHHHHHHHHHCCCCCHHHHHHEEEEEEE
IPRRQDGAGQLNIAVRQRINVFYRPPGLDGDPADTPARLLWTLTHDPSHALLLTVSNPTP
CCCCCCCCCEEEEEEEEECEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCC
YHAALQALRIDGVQLGDHLLLAPGAQVAMVVPASVERSATHRFSYQALTDYGGRRTHCAQ
HHHHHHHHHCCCEEECCEEEECCCCEEEEEECCCCCHHHHHHEEHHHHHHCCCCCEEHHH
LTGQTATTARLPKNNSIQDEC
HCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
SAFAGSCKALLTAVLLTSGLAAHGAVSLSGTRLIFDGQYREVTLEVRNRGKSEALLQAW
CCCCHHHHHHHHHHHHHCCHHHCCEEEECCCEEEECCCCEEEEEEECCCCHHHHHHHHH
LSDAADDDDAPGELRKALPFVVTPPLSRLAVGGRQALRVLYQGTGMPQKHESLLHLYVLE
HHHCCCCCCCCHHHHHCCCEEECCCHHHHHHCHHHHHHHHHHCCCCCHHHHHHEEEEEEE
IPRRQDGAGQLNIAVRQRINVFYRPPGLDGDPADTPARLLWTLTHDPSHALLLTVSNPTP
CCCCCCCCCEEEEEEEEECEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCC
YHAALQALRIDGVQLGDHLLLAPGAQVAMVVPASVERSATHRFSYQALTDYGGRRTHCAQ
HHHHHHHHHCCCEEECCEEEECCCCEEEEEECCCCCHHHHHHEEHHHHHHCCCCCEEHHH
LTGQTATTARLPKNNSIQDEC
HCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]