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The map label for this gene is estA [H]

Identifier: 66044324

GI number: 66044324

Start: 1213803

End: 1214462

Strand: Reverse

Name: estA [H]

Synonym: Psyr_1071

Alternate gene names: 66044324

Gene position: 1214462-1213803 (Counterclockwise)

Preceding gene: 66044330

Following gene: 161486737

Centisome position: 19.93

GC content: 60.76

Gene sequence:

>660_bases
ATGAGCGATTCCTTGATCATTGAACCTTCCAGCCCCGCCGACGCCTGCGTTATCTGGCTGCATGGATTGGGCGCCGACCG
CTATGATTTTCTGCCGGTCGCCGAAGCGCTGCAGGAGTCGTTGCGCACCACCCGCTTCGTACTGCCTCAGGCACCGACAC
GTGCTGTGACCGTCAATGGCGGTTATGCAATACCCAGCTGGTATGACATCAAGTCCATGAGTTCAGAGGCCAGGGCCATT
GACCATGATCAGATGGAAGCCTCGGCGCAGAAGGTGCTGGACCTGATCGAGCAGCAGCGCGACAGCGGCATCGACCCGGC
ACGAATCTTCCTGGCCGGCTTTTCCCAGGGTGGCGCGGTGGTCTTGCATGCCGGCTACCGCCGCTGGCAGGGTCCGTTGG
GCGGCGTACTCGCGCTGTCCACTTATGCCCCTACATTCAGCAACGAGATGACCCTGTCAGCGAGTCAGCAACGCATCCCG
GCCTACTGCCTGCACGGCAAGCACGACGCGGTGGTGCCTTATCCGATGGGTCGCGCCGTCTACGACCATCTCACCGCCCA
GGGTGTAACCGTTGAATGGCAGGAATACCCAATGGAGCATCAAGTGTTACCGGAGGAAATTCGCGACATCGGCGTCTGGC
TGGCTGAAAGGCTTCGCTGA

Upstream 100 bases:

>100_bases
CAAAAACTTGTAACCTTGCGACATCTTCTTTCATTTTTAATCTACCTGCGCACCTTATTAATGCGCTCAAAAAGAGCCCG
CGCACACAAATGGAGCAGTC

Downstream 100 bases:

>100_bases
AGCGCTCTGAACCCCTTGCGACGGACCGCTTTTGTAGCTCGCCATGGATGGCACTACGCCGAGCACGGTTCTTGCTTTAC
ACTGCTCGGCGTACATTCCT

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSDSLIIEPSSPADACVIWLHGLGADRYDFLPVAEALQESLRTTRFVLPQAPTRAVTVNGGYAIPSWYDIKSMSSEARAI
DHDQMEASAQKVLDLIEQQRDSGIDPARIFLAGFSQGGAVVLHAGYRRWQGPLGGVLALSTYAPTFSNEMTLSASQQRIP
AYCLHGKHDAVVPYPMGRAVYDHLTAQGVTVEWQEYPMEHQVLPEEIRDIGVWLAERLR

Sequences:

>Translated_219_residues
MSDSLIIEPSSPADACVIWLHGLGADRYDFLPVAEALQESLRTTRFVLPQAPTRAVTVNGGYAIPSWYDIKSMSSEARAI
DHDQMEASAQKVLDLIEQQRDSGIDPARIFLAGFSQGGAVVLHAGYRRWQGPLGGVLALSTYAPTFSNEMTLSASQQRIP
AYCLHGKHDAVVPYPMGRAVYDHLTAQGVTVEWQEYPMEHQVLPEEIRDIGVWLAERLR
>Mature_218_residues
SDSLIIEPSSPADACVIWLHGLGADRYDFLPVAEALQESLRTTRFVLPQAPTRAVTVNGGYAIPSWYDIKSMSSEARAID
HDQMEASAQKVLDLIEQQRDSGIDPARIFLAGFSQGGAVVLHAGYRRWQGPLGGVLALSTYAPTFSNEMTLSASQQRIPA
YCLHGKHDAVVPYPMGRAVYDHLTAQGVTVEWQEYPMEHQVLPEEIRDIGVWLAERLR

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=220, Percent_Identity=31.3636363636364, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI9966764, Length=223, Percent_Identity=29.5964125560538, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI20270341, Length=220, Percent_Identity=26.8181818181818, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17508057, Length=216, Percent_Identity=28.2407407407407, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323147, Length=218, Percent_Identity=30.2752293577982, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI45553055, Length=211, Percent_Identity=29.3838862559242, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45553057, Length=211, Percent_Identity=29.3838862559242, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21357257, Length=211, Percent_Identity=29.3838862559242, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078179, Length=221, Percent_Identity=28.0542986425339, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24236; Mature: 24104

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSLIIEPSSPADACVIWLHGLGADRYDFLPVAEALQESLRTTRFVLPQAPTRAVTVNG
CCCCEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCEEEEECC
GYAIPSWYDIKSMSSEARAIDHDQMEASAQKVLDLIEQQRDSGIDPARIFLAGFSQGGAV
CEECCCCCCHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCEE
VLHAGYRRWQGPLGGVLALSTYAPTFSNEMTLSASQQRIPAYCLHGKHDAVVPYPMGRAV
EEECCCHHHCCCCCCEEEEEECCCCCCCCEEECCCCCCCCEEEECCCCCCCCCCCCCHHH
YDHLTAQGVTVEWQEYPMEHQVLPEEIRDIGVWLAERLR
HHHHHHCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDSLIIEPSSPADACVIWLHGLGADRYDFLPVAEALQESLRTTRFVLPQAPTRAVTVNG
CCCEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCEEEEECC
GYAIPSWYDIKSMSSEARAIDHDQMEASAQKVLDLIEQQRDSGIDPARIFLAGFSQGGAV
CEECCCCCCHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCEE
VLHAGYRRWQGPLGGVLALSTYAPTFSNEMTLSASQQRIPAYCLHGKHDAVVPYPMGRAV
EEECCCHHHCCCCCCEEEEEECCCCCCCCEEECCCCCCCCEEEECCCCCCCCCCCCCHHH
YDHLTAQGVTVEWQEYPMEHQVLPEEIRDIGVWLAERLR
HHHHHHCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA