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The map label for this gene is phrB [H]

Identifier: 66044214

GI number: 66044214

Start: 1094638

End: 1096086

Strand: Reverse

Name: phrB [H]

Synonym: Psyr_0961

Alternate gene names: 66044214

Gene position: 1096086-1094638 (Counterclockwise)

Preceding gene: 66044215

Following gene: 66044213

Centisome position: 17.99

GC content: 61.08

Gene sequence:

>1449_bases
ATGCAACTGATCTGGCTGCGCAGCGACCTGCGCGCTCAAGACAACACTGCCCTGACCGCCGCCATGGAGCGCGGTCCGAC
GCTGGCGGTCTACCTGCTCAGTCCTGCGCAGTGGCGCAGCCATGACGATGCCGACTGCAAGGTAGACTTCTGGCTGCGCA
ATCTGGTCGAGCTCGAGAAGGCGCTGGGCGAGTTGAATGTTCCCTTGCTGATCCGCGAGGCGGATACCTGGGATCAGGCG
CCTGAAGTGCTGGCAACACTGTGCACACAGTTCAAGGTCGAGGGCCTGCACCTCAATCAGGAATACGGCATCAACGAAAC
CCGCCGCGACCAGGCGGTGCAGAAGGCAATGGGAAAAGCCGGTGTGCACTTCAACAGCTACCTCGATCAGCTGTTATTCA
AGCCCGGCAGCATCCTGACCAAGACCGGCAACTATTTTCAGGTATTCACCCAATTCAAGAAGGTCTGCTATACCCGACTG
CACGAGGCCATGCCGCACCCGGTGCGCACCCCGCAAGCGCAGGCGGCACAGTCGATAAAAAGCGATGCGGTTCCCAAGCA
GGTCAAGGGCTTTGCTACGCCATCGAAAGCCTTGCGTGACCTGTGGCCGGCCGGTGAGGCGGAAGCCAGACGGCGTATGG
ACGCCTTCGCCGCCGAGCAGATCAGCTACTACAAGACCGAACGCGACTTTCCGGCCAAGCCTGGCACCAGCCAGTTGTCG
GCCTATCTGGCGGCCGGGGTGATTTCGCCGCGCCAGTGCTTGCACGCAGCCCTGGCCAGCAATCAGGGTGAGTTCGAGAC
TGGCGACGTGGGCACCGTGACCTGGATCAACGAGCTATTATGGCGAGAGTTCTATAAACACACGCTGGTCGGCTACCCAC
GCGTCTCGCGTCATCGCGCCTTTCGCCCGGAAACCGAGGCGTTGAAGTGGCGCGATGCCCCAGAAGAACTGCGCGCCTGG
CAAGAAGCGCGGACCGGGCTGCCAATCATTGACGCAGCGATGCGCCAGTTGCTGGAAACCGGATGGATGCACAATCGGCT
AAGGATGGTCGTTGCGATGTTTCTCACCAAAAATCTTCTGATCGACTGGCGTGAGGGCGAGCGCTTTTTCATGCGCCACC
TGATCGACGGTGATCTGGCGTCCAACAACGGCGGCTGGCAGTGGAGCTCGTCCACTGGTACCGACTCGGCGCCCTACTTC
CGTATTTTCAATCCGCTGTCGCAGTCGGAACGCTTCGACCCCGAGGGCGTGTTCATCAAGCGCTGGCTGCCGGAACTGGC
GGATATGAATAAAAAGCAGATTCACGACCCGTCGTTAATCGGTGACCTGTTTGGCGCTGCCGACTACCCGAAACCCATCG
TCGACCTGAGCAAGAGCCGCGCCCGCGCCCTGGCAGCGTTCAAGGCGCTCCCGGCTCGACAGCCGGCCGCGGGAGTCGAA
CATGGCTGA

Upstream 100 bases:

>100_bases
GCTCGTACAGGCCAAGGAGATATCTGGACTGACCCTTGCCCACGACGTACTCAGCGCTCAGATCGAGCTGGGCAAGCTCG
GACTTATCTAAGGACTTCTC

Downstream 100 bases:

>100_bases
TTTCCTGCATCGCTTCGCCGAGGGTTTTGCCACGCTGAACAAGGACAACCTTGACCAGGTTGCCGAGCTGTACAGCGAAG
ATGTGTCGTTCAGCGACCCG

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MQLIWLRSDLRAQDNTALTAAMERGPTLAVYLLSPAQWRSHDDADCKVDFWLRNLVELEKALGELNVPLLIREADTWDQA
PEVLATLCTQFKVEGLHLNQEYGINETRRDQAVQKAMGKAGVHFNSYLDQLLFKPGSILTKTGNYFQVFTQFKKVCYTRL
HEAMPHPVRTPQAQAAQSIKSDAVPKQVKGFATPSKALRDLWPAGEAEARRRMDAFAAEQISYYKTERDFPAKPGTSQLS
AYLAAGVISPRQCLHAALASNQGEFETGDVGTVTWINELLWREFYKHTLVGYPRVSRHRAFRPETEALKWRDAPEELRAW
QEARTGLPIIDAAMRQLLETGWMHNRLRMVVAMFLTKNLLIDWREGERFFMRHLIDGDLASNNGGWQWSSSTGTDSAPYF
RIFNPLSQSERFDPEGVFIKRWLPELADMNKKQIHDPSLIGDLFGAADYPKPIVDLSKSRARALAAFKALPARQPAAGVE
HG

Sequences:

>Translated_482_residues
MQLIWLRSDLRAQDNTALTAAMERGPTLAVYLLSPAQWRSHDDADCKVDFWLRNLVELEKALGELNVPLLIREADTWDQA
PEVLATLCTQFKVEGLHLNQEYGINETRRDQAVQKAMGKAGVHFNSYLDQLLFKPGSILTKTGNYFQVFTQFKKVCYTRL
HEAMPHPVRTPQAQAAQSIKSDAVPKQVKGFATPSKALRDLWPAGEAEARRRMDAFAAEQISYYKTERDFPAKPGTSQLS
AYLAAGVISPRQCLHAALASNQGEFETGDVGTVTWINELLWREFYKHTLVGYPRVSRHRAFRPETEALKWRDAPEELRAW
QEARTGLPIIDAAMRQLLETGWMHNRLRMVVAMFLTKNLLIDWREGERFFMRHLIDGDLASNNGGWQWSSSTGTDSAPYF
RIFNPLSQSERFDPEGVFIKRWLPELADMNKKQIHDPSLIGDLFGAADYPKPIVDLSKSRARALAAFKALPARQPAAGVE
HG
>Mature_482_residues
MQLIWLRSDLRAQDNTALTAAMERGPTLAVYLLSPAQWRSHDDADCKVDFWLRNLVELEKALGELNVPLLIREADTWDQA
PEVLATLCTQFKVEGLHLNQEYGINETRRDQAVQKAMGKAGVHFNSYLDQLLFKPGSILTKTGNYFQVFTQFKKVCYTRL
HEAMPHPVRTPQAQAAQSIKSDAVPKQVKGFATPSKALRDLWPAGEAEARRRMDAFAAEQISYYKTERDFPAKPGTSQLS
AYLAAGVISPRQCLHAALASNQGEFETGDVGTVTWINELLWREFYKHTLVGYPRVSRHRAFRPETEALKWRDAPEELRAW
QEARTGLPIIDAAMRQLLETGWMHNRLRMVVAMFLTKNLLIDWREGERFFMRHLIDGDLASNNGGWQWSSSTGTDSAPYF
RIFNPLSQSERFDPEGVFIKRWLPELADMNKKQIHDPSLIGDLFGAADYPKPIVDLSKSRARALAAFKALPARQPAAGVE
HG

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=516, Percent_Identity=26.5503875968992, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI188536100, Length=481, Percent_Identity=27.6507276507277, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI188536103, Length=435, Percent_Identity=27.1264367816092, Blast_Score=142, Evalue=7e-34,
Organism=Escherichia coli, GI1786926, Length=472, Percent_Identity=44.7033898305085, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324962, Length=500, Percent_Identity=33.6, Blast_Score=276, Evalue=4e-75,
Organism=Drosophila melanogaster, GI17137248, Length=479, Percent_Identity=28.1837160751566, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24585455, Length=479, Percent_Identity=28.1837160751566, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24648152, Length=514, Percent_Identity=24.124513618677, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54639; Mature: 54639

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLIWLRSDLRAQDNTALTAAMERGPTLAVYLLSPAQWRSHDDADCKVDFWLRNLVELEK
CEEEEECCCCCCCCCHHHHHHHHCCCEEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHH
ALGELNVPLLIREADTWDQAPEVLATLCTQFKVEGLHLNQEYGINETRRDQAVQKAMGKA
HHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHC
GVHFNSYLDQLLFKPGSILTKTGNYFQVFTQFKKVCYTRLHEAMPHPVRTPQAQAAQSIK
CCHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SDAVPKQVKGFATPSKALRDLWPAGEAEARRRMDAFAAEQISYYKTERDFPAKPGTSQLS
HCCCHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
AYLAAGVISPRQCLHAALASNQGEFETGDVGTVTWINELLWREFYKHTLVGYPRVSRHRA
HHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
FRPETEALKWRDAPEELRAWQEARTGLPIIDAAMRQLLETGWMHNRLRMVVAMFLTKNLL
CCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
IDWREGERFFMRHLIDGDLASNNGGWQWSSSTGTDSAPYFRIFNPLSQSERFDPEGVFIK
EECCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHCCHHHCCCCCCCCHHHH
RWLPELADMNKKQIHDPSLIGDLFGAADYPKPIVDLSKSRARALAAFKALPARQPAAGVE
HHHHHHHCCCHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HG
CC
>Mature Secondary Structure
MQLIWLRSDLRAQDNTALTAAMERGPTLAVYLLSPAQWRSHDDADCKVDFWLRNLVELEK
CEEEEECCCCCCCCCHHHHHHHHCCCEEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHH
ALGELNVPLLIREADTWDQAPEVLATLCTQFKVEGLHLNQEYGINETRRDQAVQKAMGKA
HHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHC
GVHFNSYLDQLLFKPGSILTKTGNYFQVFTQFKKVCYTRLHEAMPHPVRTPQAQAAQSIK
CCHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SDAVPKQVKGFATPSKALRDLWPAGEAEARRRMDAFAAEQISYYKTERDFPAKPGTSQLS
HCCCHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
AYLAAGVISPRQCLHAALASNQGEFETGDVGTVTWINELLWREFYKHTLVGYPRVSRHRA
HHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
FRPETEALKWRDAPEELRAWQEARTGLPIIDAAMRQLLETGWMHNRLRMVVAMFLTKNLL
CCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
IDWREGERFFMRHLIDGDLASNNGGWQWSSSTGTDSAPYFRIFNPLSQSERFDPEGVFIK
EECCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHCCHHHCCCCCCCCHHHH
RWLPELADMNKKQIHDPSLIGDLFGAADYPKPIVDLSKSRARALAAFKALPARQPAAGVE
HHHHHHHCCCHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]