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The map label for this gene is panB [H]

Identifier: 66044083

GI number: 66044083

Start: 937690

End: 938490

Strand: Reverse

Name: panB [H]

Synonym: Psyr_0828

Alternate gene names: 66044083

Gene position: 938490-937690 (Counterclockwise)

Preceding gene: 66044084

Following gene: 66044082

Centisome position: 15.4

GC content: 63.42

Gene sequence:

>801_bases
ATGCCGAACATAACCGTTACGTCGCTGCTGGCCATGAAGCAGAAGGGTGAGAAGATCACCATGCTGACCTGCTACGACGC
AACCTTCGCCCACACGGCCTCCCAGGCGGGTGTCGAAGTGCTGCTGATAGGCGACTCGCTGGGCATGGTCCTGCAAGGTC
ACGACAGCACCCTGCCTGTTACCACCGCTGAAACGGCGTACCACGTTGCCTGTGTGAAACGCGGCAACCAGGGCGCGATG
ATTCTTGCCGACCTGCCGTTCATGGCCAACGCAACGCTGGAGCAGACCCTGATCAACAGCGCAACGCTGATGCAGGCGGG
CGCCCACATGATCAAGGTCGAAGGCGCTGCCTGGCTGGCCGAGTCGATCCGTTTGCTGGCCGAGCGCGGCATTCCGGTGT
GCGCGCACATGGGCCTGACCCCACAGGCAGTCAACGTGCTGGGCGGCTACAAGGTTCAGGGCCGGCTGGAGACGCAGGCG
CGGCAGATGCGCGCCGATGCCATCGCACTGGAACAGGCGGGCGCAGCGATGATCCTGCTGGAGTGCGTGCCCAGCGAACT
GGCCGAGGAAATCACCCAGGCGGTGAAGGTCCCGGTCATCGGCATTGGCGCAGGCAGCGCCACTGATGGTCAGGTCCTGG
TGCTGCACGACATGCTCGGCCTGTCCATCACCGGGCGCGTGCCCAAGTTCGTGAAAAACTTCATGACCGGCCAGCCCGAT
ATCCAGTCGGCTATCCGGGCCTACGTGACCGCCGTCAAAGACCTCAGCTTTCCGGCCACTGAACACGGATTCTCGGCATG
A

Upstream 100 bases:

>100_bases
GCCTTTGCAAAACACCGCGACACGACGGTGTGCCTGCTTTTCCAGGATGACTCTCACGCGCTGAGCACCCGTCTTCACAG
CGCCTGATGGAGGACTTTAC

Downstream 100 bases:

>100_bases
ACACAGTAAAAACCTTACAGGACCTGCGCGCCGCCGTTGCGCGGGCACGCAGCGAAGGCAAGCGGATCGCGCTGACCCCG
ACGATGGGCAACCTGCACAG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MPNITVTSLLAMKQKGEKITMLTCYDATFAHTASQAGVEVLLIGDSLGMVLQGHDSTLPVTTAETAYHVACVKRGNQGAM
ILADLPFMANATLEQTLINSATLMQAGAHMIKVEGAAWLAESIRLLAERGIPVCAHMGLTPQAVNVLGGYKVQGRLETQA
RQMRADAIALEQAGAAMILLECVPSELAEEITQAVKVPVIGIGAGSATDGQVLVLHDMLGLSITGRVPKFVKNFMTGQPD
IQSAIRAYVTAVKDLSFPATEHGFSA

Sequences:

>Translated_266_residues
MPNITVTSLLAMKQKGEKITMLTCYDATFAHTASQAGVEVLLIGDSLGMVLQGHDSTLPVTTAETAYHVACVKRGNQGAM
ILADLPFMANATLEQTLINSATLMQAGAHMIKVEGAAWLAESIRLLAERGIPVCAHMGLTPQAVNVLGGYKVQGRLETQA
RQMRADAIALEQAGAAMILLECVPSELAEEITQAVKVPVIGIGAGSATDGQVLVLHDMLGLSITGRVPKFVKNFMTGQPD
IQSAIRAYVTAVKDLSFPATEHGFSA
>Mature_265_residues
PNITVTSLLAMKQKGEKITMLTCYDATFAHTASQAGVEVLLIGDSLGMVLQGHDSTLPVTTAETAYHVACVKRGNQGAMI
LADLPFMANATLEQTLINSATLMQAGAHMIKVEGAAWLAESIRLLAERGIPVCAHMGLTPQAVNVLGGYKVQGRLETQAR
QMRADAIALEQAGAAMILLECVPSELAEEITQAVKVPVIGIGAGSATDGQVLVLHDMLGLSITGRVPKFVKNFMTGQPDI
QSAIRAYVTAVKDLSFPATEHGFSA

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=264, Percent_Identity=52.6515151515151, Blast_Score=304, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6319653, Length=252, Percent_Identity=39.2857142857143, Blast_Score=175, Evalue=5e-45,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 28041; Mature: 27910

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNITVTSLLAMKQKGEKITMLTCYDATFAHTASQAGVEVLLIGDSLGMVLQGHDSTLPV
CCCCHHHHHHHHHHCCCEEEEEEEECCHHHHHHHCCCEEEEEEECCCCEEEECCCCCCCE
TTAETAYHVACVKRGNQGAMILADLPFMANATLEQTLINSATLMQAGAHMIKVEGAAWLA
EECCHHEEEEEEEECCCCCEEEEECCCHHCCHHHHHHHHHHHHHHCCCEEEEECHHHHHH
ESIRLLAERGIPVCAHMGLTPQAVNVLGGYKVQGRLETQARQMRADAIALEQAGAAMILL
HHHHHHHHCCCCEEEECCCCHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEE
ECVPSELAEEITQAVKVPVIGIGAGSATDGQVLVLHDMLGLSITGRVPKFVKNFMTGQPD
HHCCHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEECCCCEECCCCHHHHHHHHCCCCC
IQSAIRAYVTAVKDLSFPATEHGFSA
HHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PNITVTSLLAMKQKGEKITMLTCYDATFAHTASQAGVEVLLIGDSLGMVLQGHDSTLPV
CCCHHHHHHHHHHCCCEEEEEEEECCHHHHHHHCCCEEEEEEECCCCEEEECCCCCCCE
TTAETAYHVACVKRGNQGAMILADLPFMANATLEQTLINSATLMQAGAHMIKVEGAAWLA
EECCHHEEEEEEEECCCCCEEEEECCCHHCCHHHHHHHHHHHHHHCCCEEEEECHHHHHH
ESIRLLAERGIPVCAHMGLTPQAVNVLGGYKVQGRLETQARQMRADAIALEQAGAAMILL
HHHHHHHHCCCCEEEECCCCHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEE
ECVPSELAEEITQAVKVPVIGIGAGSATDGQVLVLHDMLGLSITGRVPKFVKNFMTGQPD
HHCCHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEECCCCEECCCCHHHHHHHHCCCCC
IQSAIRAYVTAVKDLSFPATEHGFSA
HHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA