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The map label for this gene is fruR [H]

Identifier: 66044075

GI number: 66044075

Start: 925431

End: 926426

Strand: Reverse

Name: fruR [H]

Synonym: Psyr_0820

Alternate gene names: 66044075

Gene position: 926426-925431 (Counterclockwise)

Preceding gene: 66044079

Following gene: 66044074

Centisome position: 15.2

GC content: 59.34

Gene sequence:

>996_bases
GTGAAACTGAGCGATATCGCACGTCTGGCAGGTGTTTCTGTCACTACGGCCAGTTACGTCATCAATGGCAAGGCCGAAAA
ACAGCGTATCAGCCCGGCAACGGTCGAGCGTGTCAAAGCCGTGGTCGAGCAGCACGACTTTCGGCCCAACCCGCAAGCGG
CGGGCCTGCGCAGCCGTCATAGCAAGACACTGGGCTTTATCCTGCCCGACCTGGAAAACCCCAGCTATGCGCGTATCGCC
AAATTGCTCGAGCAAGGCGCGCGTAGACTCGGTTATCAACTCCTTATCGCCAGTTCTGATGACGACCCCGCCAGCGAGCT
GCAGCTTCTGCAGCTGTTCCGAGCACGCCGTTGTGATGCACTGCTGGTCGCCAGTTGCCTGCCGACCGGTGACGACAGCT
ACCGCGAATTGCAGCTCAAGGGCACGCCGATCATCGCCATCGACCGGGAAATGAATCCCGAGTTTTTCTGCTCGGTGGTC
AGCGACGACCACGACGCCTGTCAGCAACTGACCCACAGCCTCCTGCAAACCCGACCGCGACACATCGCCCTGATCAGCGC
ACGTCCTGAACTGAGCATCAGTCGCGCGCGTGCCGCCGGCTTCGAACACGCACTCGACGGGTTTACCGGAGCCACCATCA
TTGAGCATGGCGAAGCGTTCAGCCGCCAGTGTGGTCAGCGTTTGATGACCGAAATGTTACAGCGCCAGGGACATCTTCCG
GACGCGTTGATCACCACGTCCTACGTGCTGCTGCAAGGCGTGTTCGATGTACTGCAAAGCCAGGCACTGCAATCAACCCA
ATTGCATCTGGGCACTTTCGGCGATACTCAATTGCTGGATTTCCTGCCGCTGCCCGTCAACGCCATGGCCCAGCAACACC
AATTGATCGCCGACAAAGCCCTGCAACTGGCGCTGGCGGCTATCGAGAAGGATGACTACCAGCCAGGCGTGCATGCGATT
GCCAGGGCCTTCAAGCAACGTATTCACGAGGCGTGA

Upstream 100 bases:

>100_bases
AGTACTTGGGAATTTCCTACGGTCTGGTGTAAAGATCAGCGCCATGAAAAAAATAACTCGATTGTCCTGAGCCGCGTACC
TAATAAAAGGAAATTCCGGG

Downstream 100 bases:

>100_bases
ATGTGACGCTGATCGATACCCACACCCACCTGGATTTTCCGGACTTCGATGCCGACCGCACTCAGGTGCTGGAAAACTGC
CTGGCGCTCGGCGTGCAGCG

Product: DNA-binding transcriptional regulator FruR

Products: NA

Alternate protein names: Catabolite repressor/activator [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKLSDIARLAGVSVTTASYVINGKAEKQRISPATVERVKAVVEQHDFRPNPQAAGLRSRHSKTLGFILPDLENPSYARIA
KLLEQGARRLGYQLLIASSDDDPASELQLLQLFRARRCDALLVASCLPTGDDSYRELQLKGTPIIAIDREMNPEFFCSVV
SDDHDACQQLTHSLLQTRPRHIALISARPELSISRARAAGFEHALDGFTGATIIEHGEAFSRQCGQRLMTEMLQRQGHLP
DALITTSYVLLQGVFDVLQSQALQSTQLHLGTFGDTQLLDFLPLPVNAMAQQHQLIADKALQLALAAIEKDDYQPGVHAI
ARAFKQRIHEA

Sequences:

>Translated_331_residues
MKLSDIARLAGVSVTTASYVINGKAEKQRISPATVERVKAVVEQHDFRPNPQAAGLRSRHSKTLGFILPDLENPSYARIA
KLLEQGARRLGYQLLIASSDDDPASELQLLQLFRARRCDALLVASCLPTGDDSYRELQLKGTPIIAIDREMNPEFFCSVV
SDDHDACQQLTHSLLQTRPRHIALISARPELSISRARAAGFEHALDGFTGATIIEHGEAFSRQCGQRLMTEMLQRQGHLP
DALITTSYVLLQGVFDVLQSQALQSTQLHLGTFGDTQLLDFLPLPVNAMAQQHQLIADKALQLALAAIEKDDYQPGVHAI
ARAFKQRIHEA
>Mature_331_residues
MKLSDIARLAGVSVTTASYVINGKAEKQRISPATVERVKAVVEQHDFRPNPQAAGLRSRHSKTLGFILPDLENPSYARIA
KLLEQGARRLGYQLLIASSDDDPASELQLLQLFRARRCDALLVASCLPTGDDSYRELQLKGTPIIAIDREMNPEFFCSVV
SDDHDACQQLTHSLLQTRPRHIALISARPELSISRARAAGFEHALDGFTGATIIEHGEAFSRQCGQRLMTEMLQRQGHLP
DALITTSYVLLQGVFDVLQSQALQSTQLHLGTFGDTQLLDFLPLPVNAMAQQHQLIADKALQLALAAIEKDDYQPGVHAI
ARAFKQRIHEA

Specific function: Represses some operons but activates others. It is implicated in the regulation of a large number of operons encoding enzymes which comprise central pathways of carbon metabolism. Binds D-fructose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=329, Percent_Identity=42.2492401215805, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1787906, Length=202, Percent_Identity=31.6831683168317, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789202, Length=238, Percent_Identity=29.4117647058824, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=23.3333333333333, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787948, Length=274, Percent_Identity=23.7226277372263, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788474, Length=287, Percent_Identity=25.4355400696864, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=24.9190938511327, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789068, Length=304, Percent_Identity=25.3289473684211, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI48994940, Length=204, Percent_Identity=24.5098039215686, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787580, Length=269, Percent_Identity=23.4200743494424, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36529; Mature: 36529

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSDIARLAGVSVTTASYVINGKAEKQRISPATVERVKAVVEQHDFRPNPQAAGLRSRH
CCHHHHHHHHCCEEEHHHHEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
SKTLGFILPDLENPSYARIAKLLEQGARRLGYQLLIASSDDDPASELQLLQLFRARRCDA
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCH
LLVASCLPTGDDSYRELQLKGTPIIAIDREMNPEFFCSVVSDDHDACQQLTHSLLQTRPR
HHHHHHCCCCCCCCEEEEECCCEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCC
HIALISARPELSISRARAAGFEHALDGFTGATIIEHGEAFSRQCGQRLMTEMLQRQGHLP
EEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
DALITTSYVLLQGVFDVLQSQALQSTQLHLGTFGDTQLLDFLPLPVNAMAQQHQLIADKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
LQLALAAIEKDDYQPGVHAIARAFKQRIHEA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLSDIARLAGVSVTTASYVINGKAEKQRISPATVERVKAVVEQHDFRPNPQAAGLRSRH
CCHHHHHHHHCCEEEHHHHEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
SKTLGFILPDLENPSYARIAKLLEQGARRLGYQLLIASSDDDPASELQLLQLFRARRCDA
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCH
LLVASCLPTGDDSYRELQLKGTPIIAIDREMNPEFFCSVVSDDHDACQQLTHSLLQTRPR
HHHHHHCCCCCCCCEEEEECCCEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCC
HIALISARPELSISRARAAGFEHALDGFTGATIIEHGEAFSRQCGQRLMTEMLQRQGHLP
EEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
DALITTSYVLLQGVFDVLQSQALQSTQLHLGTFGDTQLLDFLPLPVNAMAQQHQLIADKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
LQLALAAIEKDDYQPGVHAIARAFKQRIHEA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]