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The map label for this gene is pilB [H]

Identifier: 66044053

GI number: 66044053

Start: 903509

End: 905203

Strand: Reverse

Name: pilB [H]

Synonym: Psyr_0798

Alternate gene names: 66044053

Gene position: 905203-903509 (Counterclockwise)

Preceding gene: 66044055

Following gene: 66044052

Centisome position: 14.85

GC content: 53.45

Gene sequence:

>1695_bases
ATGACTGATGCTGTCCTCACTGGCTTGGCCAAGCAATTGGTCCTCGCCGAGCTACTTACTGAACCTGTTGCGCAGCAAGC
GTATCAGCAGGCGCGCCGCGACAAGATTTCGTTGGTGAGCTATCTGGTTCAGCACAAACTGGTCAAAAGCCTGACGCTGG
CAGAACTGGCCTCCGATCAGTTCGGCGTGCCTTTCATGGACCTCGCCAGCCTGGACAAGGAAAGCCTGCCTAAAGGCTTG
GTCAGTGAAAAGCTGGTTCGACAACATCACGCACTGCCGTTGTGGCGACGCGGTAACAAACTGTTTATCGGGATTTCGGA
CCCGACCAATCATCAGGCAGTGACTGAGATCCAGTTCAGCACTGGCCTGAACACCGAAGCGATTCTGGTTGAAGATGACA
AGCTGACTGTTGCGATAGACCGGTTTTTTGACAGCGACAGCGGGCTGGGCAATCTGGAAGATGTCGATCTGGGCCTGGAT
ATCGAACCGGCCGATGGCAAGGAAACCACGCTAGCTACACAGAATGACGCTGACGATGCCCCGGTGGTGCGCTTCGTCAA
CAAGATGCTTATGGATGCCATCCGACTAGGATCATCCGACTTGCACTTCGAGCCCTATGAAAAGATCTTCCGCGTACGTT
TGCGTACCGATGGCATCCTGCACGAAGTCGCCAGACCGCCGATCCATTTGGCAAACCGTATTGCTGCGCGTCTGAAGGTC
ATGGCCAGCCTCGATATTTCGGAACGACGCAAACCGCAGGACGGAAGGGTCAAGTTGCGGGTGTCCAAAACAAAGGCCAT
CGATTTTCGTATGAATACCCTGCCCACATTGTGGGGCGAGAAGATCGTAATGCGGATTCTCGACCCAACCAGTGCGCAGA
TGGGTATCGATGCGCTGGGCTATGAGCCTGATCAAAAGGCACTGTACTTAGAGGCGCTTAAACTGCCGCAAGGGATGATT
CTTGTGACTGGCCCTACAGGTTCAGGCAAGACTGTATCTCTCTATACCGGCCTGAACATTCTAAACACTGTGGACATTAA
CATTTCCACAGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGCCAAGGACTGG
ACTTTTCCCAAGCCTTGCGCGCGTTTTTGCGTCAAGATCCCGACGTGATTATGGTCGGCGAAATACGAGATCTGGAAACT
GCCGAAATCGCCATCAAAGCCTCGCAGACCGGCCATATGGTGCTTTCCACATTGCACACCAACAGCGCTGCGGAAACGCT
GACCCGCCTGCATCACATGGGCGTTGCAGCATTCAACATTGCTACAGCGATCAACCTGATTATTGCGCAGCGACTGGCAC
GTAAGCTGTGTAGCCACTGCAAAAAAGAACTGGATATTCCTCGCGAGACACTGATTCAGGAAGGCTTTCCAGAAGCCAAA
ATCGGCACTTTTAAGGTATACGGCCCGGTGGGCTGTGAGCACTGCAACGGGGGTTACCGAGGCAGGGTGGGCATTTACGA
AGTGGTCAAGAAAACACCGGAACTGGAACGCATCATCATGGAAGAAGGCAACTCCCTGGAGATCTCCAGACAGATGCGCA
AGGACGGTTTCAATGACCTGCGAACCTCAGGCCTGTCAAAAGCTATGCAGGGCATTACCAGCCTTGAAGAAGTCAACCGT
GTGACCAAGGATTAA

Upstream 100 bases:

>100_bases
ATCCGCAGAAACTGACAATTTTCGTCACATCCCTCACTGCTATTTGGCAGCAAAGCCCGACTGCGCTATAACCCACACAT
TGTCCGTGTCTGGTGGTTTT

Downstream 100 bases:

>100_bases
ACATGGCCAGCAAGGCAGCAAAAGTCATCGTTTACACTTGGGAAGGTGTTGACAAGAAAGGTACTAAAACCAGTGGGGAA
CTGAGTGGCCATAACTTGGC

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQHKLVKSLTLAELASDQFGVPFMDLASLDKESLPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTEIQFSTGLNTEAILVEDDKLTVAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETTLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKALYLEALKLPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEAK
IGTFKVYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Sequences:

>Translated_564_residues
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQHKLVKSLTLAELASDQFGVPFMDLASLDKESLPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTEIQFSTGLNTEAILVEDDKLTVAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETTLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKALYLEALKLPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEAK
IGTFKVYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD
>Mature_563_residues
TDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQHKLVKSLTLAELASDQFGVPFMDLASLDKESLPKGLV
SEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTEIQFSTGLNTEAILVEDDKLTVAIDRFFDSDSGLGNLEDVDLGLDI
EPADGKETTLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKVM
ASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKALYLEALKLPQGMIL
VTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLETA
EIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEAKI
GTFKVYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNRV
TKD

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=415, Percent_Identity=45.3012048192771, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1786296, Length=402, Percent_Identity=44.2786069651741, Blast_Score=344, Evalue=1e-95,
Organism=Escherichia coli, GI87082188, Length=183, Percent_Identity=37.7049180327869, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62452; Mature: 62321

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQHKLVKSLTLAELASDQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FGVPFMDLASLDKESLPKGLVSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTEIQFS
CCCCHHHHHHCCHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCEEEEEEEC
TGLNTEAILVEDDKLTVAIDRFFDSDSGLGNLEDVDLGLDIEPADGKETTLATQNDADDA
CCCCCCEEEEECCCEEEEEEEHCCCCCCCCCCCCCCCCCEECCCCCCCCEEECCCCCCCC
PVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHH
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALG
HHHCCHHHCCCCCCCCEEEEEECCEEEEEECCCCCHHHHHHHHHHHCCCCCHHCCCHHCC
YEPDQKALYLEALKLPQGMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLE
CCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCEEEEEEEEEECCCCCEEEEEE
GINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLETAEIAIKASQTGHMVLSTLHT
CCCEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEEECCCCCEEEEEHHC
NSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEAK
CHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
IGTFKVYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDL
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCCHHH
RTSGLSKAMQGITSLEEVNRVTKD
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQHKLVKSLTLAELASDQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FGVPFMDLASLDKESLPKGLVSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTEIQFS
CCCCHHHHHHCCHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCEEEEEEEC
TGLNTEAILVEDDKLTVAIDRFFDSDSGLGNLEDVDLGLDIEPADGKETTLATQNDADDA
CCCCCCEEEEECCCEEEEEEEHCCCCCCCCCCCCCCCCCEECCCCCCCCEEECCCCCCCC
PVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHH
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALG
HHHCCHHHCCCCCCCCEEEEEECCEEEEEECCCCCHHHHHHHHHHHCCCCCHHCCCHHCC
YEPDQKALYLEALKLPQGMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLE
CCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCEEEEEEEEEECCCCCEEEEEE
GINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLETAEIAIKASQTGHMVLSTLHT
CCCEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEEECCCCCEEEEEHHC
NSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEAK
CHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
IGTFKVYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDL
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCCHHH
RTSGLSKAMQGITSLEEVNRVTKD
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]