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The map label for this gene is sacA [H]

Identifier: 66044013

GI number: 66044013

Start: 863082

End: 864563

Strand: Reverse

Name: sacA [H]

Synonym: Psyr_0758

Alternate gene names: 66044013

Gene position: 864563-863082 (Counterclockwise)

Preceding gene: 66044014

Following gene: 66044012

Centisome position: 14.19

GC content: 60.73

Gene sequence:

>1482_bases
ATGAGCAACCCGATGTCCCCCGCCCTCGAACGCGCGCACCATGCGCTGGAAGAGGGCCTGTCGCAGCTCATCCACGATTA
TCGGCCCGGTTACCACCTGGCCCCGCCGGCAGGCTGGATGAATGACCCCAACGGGGTGGTGTTCTTTCGTGGCGAATATC
ATGTGTTCTATCAACACCACCCGTTCGACGCGAAATGGGGCCCGATGTACTGGGGCCATGCAAAGAGTGCCGATCTGGTC
CATTGGCAACACCTGCCCATTGCCCTGGCACCCGGTGATGACTTCGATCGGGACGGTTGTTTTTCCGGCAGTGCAGTGGT
GTGTGGCGACACCCTGGCGCTGATCTACACCGGGCACATATGGCTGGGTGACGTGGGCGACGAGCGTTTTATCCGTCAGG
TGCAGTGCCTGGCCACCAGCACCGATGGCATCAGTTTCGTCAAACACGGGGCCGTTATCGACACCCCGCCGCAAGACGAG
ATGATTCATTTCCGCGACCCGAAAGTCTGGCGACAGGATGGCCACTGGTACCTGATTGCCGGCGCGCGCCTGGGCGACAA
ACCGCTGCTGCCGCTCTATCGATCCAAGGACCTGCACGCGTGGGAATTCGTCAGCTATGTGTCATCAGGCGAGGAAGGCG
ACGGCTATATGTGGGAATGCCCGGACCTGTTTGGCCTCAATGAACGCGACGTACTGCTTTACTCCCCCCAGGGCATGCCG
GCACAGCGCTACGAGCGACTGAACAGGTTCCACACCGGCTATCGAGTGGGCCAGGTCGACAGTCAATGGCAGTTCACCGG
CGGGCCTTTTATCGAGCTGGACAGCGGCCACGACTTCTATGCGGCGCAGACGCTGGTGGCGGCAGATGGCAGACGTCTGC
TGTGGGCGTGGATCGACATGTGGGAAAGCCCGACCCCCACCGAAGCGCATCACTGGCGCGGCATGCTCGGCCTGCCGCGA
GAACTTGAAGTGCGCGAAAATCGCCTCTGCGTACACCCCGCACGCGAGCTGACAGCGTTGCGCGACGCGTCACTGCCAGG
CACTGCCTGGTGGAGCGACGCGGGTAGCCAGTGGCTGACCGACATACACGGCGACATGCTGGAAATCCACGTGCAGTTGG
ACCTGCTCGACTGCATCGAAGGCCACTTGGGCGTGGCATTGCGCTGCAGTCCAGATGGCCAGGAGCAGACTCTGCTTTAC
TACGATGCGTCGCTAAAACGTTTGATCCTCGACCGTACTCAGTCGGGCACCCACGTCAGCGGTCAGCGCAGCGTAGCGAT
AGATCCACAACAGAATCAGCTTGAACTGCGGATCTTCCTTGATCGCTCGTCCATTGAGGTGTTCGAGCAAAACGGCAGTT
TCAGCTTCAGCAGCCGCCTTTATCCACAGACCGACAGCCTGGGGGTCAAGCTTGTTGCCAACGGCACAGGAGGCCGAGTC
TGCATTGCCAATGCATGGCAGCTCTCCTCCGGATACCTGTAA

Upstream 100 bases:

>100_bases
TGGCCCTGAAGCGCCACACAGACGCCAGTCAACACCTGACACCCGGCACCACACCGCACCTCGTACAAGCAGGCACCCTA
TGAGTTTTTCCGTGAATGCC

Downstream 100 bases:

>100_bases
GGTAAATCATTGAAGTCGCGAAAAAAATCAGCTCTCTGTAGCATGATAATCATCAGTCAACCCGACAGGCCCCGACCCGC
ATGACCTCAGTTAAAGACGT

Product: sucrose-6-phosphate hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSNPMSPALERAHHALEEGLSQLIHDYRPGYHLAPPAGWMNDPNGVVFFRGEYHVFYQHHPFDAKWGPMYWGHAKSADLV
HWQHLPIALAPGDDFDRDGCFSGSAVVCGDTLALIYTGHIWLGDVGDERFIRQVQCLATSTDGISFVKHGAVIDTPPQDE
MIHFRDPKVWRQDGHWYLIAGARLGDKPLLPLYRSKDLHAWEFVSYVSSGEEGDGYMWECPDLFGLNERDVLLYSPQGMP
AQRYERLNRFHTGYRVGQVDSQWQFTGGPFIELDSGHDFYAAQTLVAADGRRLLWAWIDMWESPTPTEAHHWRGMLGLPR
ELEVRENRLCVHPARELTALRDASLPGTAWWSDAGSQWLTDIHGDMLEIHVQLDLLDCIEGHLGVALRCSPDGQEQTLLY
YDASLKRLILDRTQSGTHVSGQRSVAIDPQQNQLELRIFLDRSSIEVFEQNGSFSFSSRLYPQTDSLGVKLVANGTGGRV
CIANAWQLSSGYL

Sequences:

>Translated_493_residues
MSNPMSPALERAHHALEEGLSQLIHDYRPGYHLAPPAGWMNDPNGVVFFRGEYHVFYQHHPFDAKWGPMYWGHAKSADLV
HWQHLPIALAPGDDFDRDGCFSGSAVVCGDTLALIYTGHIWLGDVGDERFIRQVQCLATSTDGISFVKHGAVIDTPPQDE
MIHFRDPKVWRQDGHWYLIAGARLGDKPLLPLYRSKDLHAWEFVSYVSSGEEGDGYMWECPDLFGLNERDVLLYSPQGMP
AQRYERLNRFHTGYRVGQVDSQWQFTGGPFIELDSGHDFYAAQTLVAADGRRLLWAWIDMWESPTPTEAHHWRGMLGLPR
ELEVRENRLCVHPARELTALRDASLPGTAWWSDAGSQWLTDIHGDMLEIHVQLDLLDCIEGHLGVALRCSPDGQEQTLLY
YDASLKRLILDRTQSGTHVSGQRSVAIDPQQNQLELRIFLDRSSIEVFEQNGSFSFSSRLYPQTDSLGVKLVANGTGGRV
CIANAWQLSSGYL
>Mature_492_residues
SNPMSPALERAHHALEEGLSQLIHDYRPGYHLAPPAGWMNDPNGVVFFRGEYHVFYQHHPFDAKWGPMYWGHAKSADLVH
WQHLPIALAPGDDFDRDGCFSGSAVVCGDTLALIYTGHIWLGDVGDERFIRQVQCLATSTDGISFVKHGAVIDTPPQDEM
IHFRDPKVWRQDGHWYLIAGARLGDKPLLPLYRSKDLHAWEFVSYVSSGEEGDGYMWECPDLFGLNERDVLLYSPQGMPA
QRYERLNRFHTGYRVGQVDSQWQFTGGPFIELDSGHDFYAAQTLVAADGRRLLWAWIDMWESPTPTEAHHWRGMLGLPRE
LEVRENRLCVHPARELTALRDASLPGTAWWSDAGSQWLTDIHGDMLEIHVQLDLLDCIEGHLGVALRCSPDGQEQTLLYY
DASLKRLILDRTQSGTHVSGQRSVAIDPQQNQLELRIFLDRSSIEVFEQNGSFSFSSRLYPQTDSLGVKLVANGTGGRVC
IANAWQLSSGYL

Specific function: Unknown

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=492, Percent_Identity=25.8130081300813, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 55710; Mature: 55579

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPMSPALERAHHALEEGLSQLIHDYRPGYHLAPPAGWMNDPNGVVFFRGEYHVFYQHH
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEECCCEEEEEEEC
PFDAKWGPMYWGHAKSADLVHWQHLPIALAPGDDFDRDGCFSGSAVVCGDTLALIYTGHI
CCCCCCCCEECCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCEEEECCEEEEEEECCE
WLGDVGDERFIRQVQCLATSTDGISFVKHGAVIDTPPQDEMIHFRDPKVWRQDGHWYLIA
EEECCCCHHHHHHHHHHCCCCCCHHHHCCCCEECCCCCCCEEEECCCCCEEECCCEEEEE
GARLGDKPLLPLYRSKDLHAWEFVSYVSSGEEGDGYMWECPDLFGLNERDVLLYSPQGMP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEECCCCCC
AQRYERLNRFHTGYRVGQVDSQWQFTGGPFIELDSGHDFYAAQTLVAADGRRLLWAWIDM
HHHHHHHHHHHCCEEECCCCCCEEECCCCEEEECCCCCCHHHEEEEECCCCEEEEEEHHH
WESPTPTEAHHWRGMLGLPRELEVRENRLCVHPARELTALRDASLPGTAWWSDAGSQWLT
CCCCCCCCHHHCCCCCCCCCCEEECCCEEEECCHHHHHHHHCCCCCCCCEECCCCCHHHH
DIHGDMLEIHVQLDLLDCIEGHLGVALRCSPDGQEQTLLYYDASLKRLILDRTQSGTHVS
HHCCCEEEEEEEEEHHHHCCCCCCEEEEECCCCCCCEEEEECCHHHHHHHHCCCCCCEEC
GQRSVAIDPQQNQLELRIFLDRSSIEVFEQNGSFSFSSRLYPQTDSLGVKLVANGTGGRV
CCCEEEECCCCCCEEEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCEEEEECCCCCEE
CIANAWQLSSGYL
EEEEEEECCCCCC
>Mature Secondary Structure 
SNPMSPALERAHHALEEGLSQLIHDYRPGYHLAPPAGWMNDPNGVVFFRGEYHVFYQHH
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEECCCEEEEEEEC
PFDAKWGPMYWGHAKSADLVHWQHLPIALAPGDDFDRDGCFSGSAVVCGDTLALIYTGHI
CCCCCCCCEECCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCEEEECCEEEEEEECCE
WLGDVGDERFIRQVQCLATSTDGISFVKHGAVIDTPPQDEMIHFRDPKVWRQDGHWYLIA
EEECCCCHHHHHHHHHHCCCCCCHHHHCCCCEECCCCCCCEEEECCCCCEEECCCEEEEE
GARLGDKPLLPLYRSKDLHAWEFVSYVSSGEEGDGYMWECPDLFGLNERDVLLYSPQGMP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEECCCCCC
AQRYERLNRFHTGYRVGQVDSQWQFTGGPFIELDSGHDFYAAQTLVAADGRRLLWAWIDM
HHHHHHHHHHHCCEEECCCCCCEEECCCCEEEECCCCCCHHHEEEEECCCCEEEEEEHHH
WESPTPTEAHHWRGMLGLPRELEVRENRLCVHPARELTALRDASLPGTAWWSDAGSQWLT
CCCCCCCCHHHCCCCCCCCCCEEECCCEEEECCHHHHHHHHCCCCCCCCEECCCCCHHHH
DIHGDMLEIHVQLDLLDCIEGHLGVALRCSPDGQEQTLLYYDASLKRLILDRTQSGTHVS
HHCCCEEEEEEEEEHHHHCCCCCCEEEEECCCCCCCEEEEECCHHHHHHHHCCCCCCEEC
GQRSVAIDPQQNQLELRIFLDRSSIEVFEQNGSFSFSSRLYPQTDSLGVKLVANGTGGRV
CCCEEEECCCCCCEEEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCEEEEECCCCCEE
CIANAWQLSSGYL
EEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2254250; 1368686 [H]