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The map label for this gene is pilS [H]

Identifier: 66043988

GI number: 66043988

Start: 827353

End: 828945

Strand: Reverse

Name: pilS [H]

Synonym: Psyr_0723

Alternate gene names: 66043988

Gene position: 828945-827353 (Counterclockwise)

Preceding gene: 66043989

Following gene: 66043987

Centisome position: 13.6

GC content: 60.08

Gene sequence:

>1593_bases
GTGACCGCTGACTCACCGTCCCTTGTCAGCCCGCAGGCACAGCGGATTCTACGGCTGTACCACCTCTACCGACTGACCAT
CGGCGTTGTACTGGTTCTGTTGATTTCCAGCAGCCTGGAAACCGAGCTGCTGCAACTGGCCAACCCGAACCTGTTCCGCT
CGGGCTGCTGGTTGTATCTGGTGTTCAACATTCTGGTCGTCGTGCTGCTGGAACGCCCCAGCCGTCAGGCACAGTTTTTC
GGCCTGGCGATGACCGACGCGATCATGCTCTCCGCGCTATTCTACGCAGCCGGCGGGCCTTCCAGCGGTATCGGCAACCT
GTTGATTGCCTCGGTGGCGATCAGCAACGTGCTGCTGCGCGGCCGTATCGGCTTGTTGATCGCTGCGGTTTCGGCCATCG
GCATCATCTACTTGACGTTCTACATCAGCTTCGGCACACCCTCGGTTTCCAACGACTACGTGCAGGCTGGCTCGCTGGGC
GCCTTGTGCTTCGCCGCCGCCCTGCTGGTGCAGGCGCTGACAGCGCGCATGCAGGTCAGCGAGGTACTGGCCGAACAGCG
CGCGGCGGATGTCGACAGCCTCGAAGCACTCAACGCACTCATTCTGCAACGCATGCGTACCGGCATTCTGGTGCTGGATG
CCAATCACCAGGTGCTGCTGGCCAATCAGGGTGCCTTACACCTGCTGGGTCAGGAGCAACTGGTCGGCCAGATCATCGAC
ACCTGCTCGCCACAACTGGTCGAACGCCTGCGCCAGTGGCTCGTCAACCCGATCATGGTGCCGCGCAGCATCACCACCGC
CCCCGTCGGGCTGGTGCTGCAACCGAGCTTTGCCACACTCAACCGTGGCGATCAGCGTCAGACGCTGGTTTTTCTCGAAG
ACCTGTCGCAGATCGCCCATCAGGCGCAGCAGCTCAAGCTTGCCGCGCTGGGGCGCCTGACCGCCGGGATTGCCCATGAG
ATACGCAACCCGCTGGGCGCCGTCAGTCATGCGGCGCAGTTGCTTAATGAATCGGAAGAGCTCAGTGCAGCGGATCGACG
CTTGGGGCAGATCATTCATGAGCAGTCGGGACGCATTGATAGGGTCATCGAAAATGTCCTGCAGTTGTCACGCAGACGCG
AAGCGCAACCCGAACTGCTCGATCTGAAAACCTGGCTGGAACACTTTGTCGCTGAATTTCGCAGTTCCGCTGCGCCCAAT
CAGAAGATTCATGTCGACATCAGCCCTGGCACGCTGACAACCCGCATGGATTCCGGACAACTGACGCAAATCATGACCAA
CCTGGTGCAGAACGGCCTGCGCTACAGCGGCAAGAATCATCCGTCAGCGCAGGTCTGGCTGAAACTACACAATGACCAGA
AGAGCGACCTGCCCACACTGGAAGTGCTGGACGACGGGCCGGGGGTCAGCGAGCAACACCTGAGCAAAATGTTCGAACCG
TTTTTCACCACAGAAAGCAAAGGCACCGGCCTTGGTCTATATCTGTCACGTGAACTGTGTGAAAGCAATCAGGCGCATCT
GGAATACGCGCCCCGCCCCACGGGTGGCAGTTGCCTGCGCATTACCTTCGCCCACCCGTTCAAGACGAGCTGA

Upstream 100 bases:

>100_bases
TGCGCGCAGTGTGGCGTTCATCTGCCGCAGGATCGTGCGCTGAGCCAGGCACAGCAATGGTATTGCAGCGAAGCACACCG
ACTGCAAGGCCCCGCGGCAC

Downstream 100 bases:

>100_bases
GCATGAGCCAACGGCAAAAAATCCTGATAGTCGATGATGAGCCGGATATTCGCGAACTCCTGGAGATCACGCTGGGACGC
ATGAAACTCGACACCCGTAG

Product: type IV pilus sensor protein PilS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTADSPSLVSPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANPNLFRSGCWLYLVFNILVVVLLERPSRQAQFF
GLAMTDAIMLSALFYAAGGPSSGIGNLLIASVAISNVLLRGRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLG
ALCFAAALLVQALTARMQVSEVLAEQRAADVDSLEALNALILQRMRTGILVLDANHQVLLANQGALHLLGQEQLVGQIID
TCSPQLVERLRQWLVNPIMVPRSITTAPVGLVLQPSFATLNRGDQRQTLVFLEDLSQIAHQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLNESEELSAADRRLGQIIHEQSGRIDRVIENVLQLSRRREAQPELLDLKTWLEHFVAEFRSSAAPN
QKIHVDISPGTLTTRMDSGQLTQIMTNLVQNGLRYSGKNHPSAQVWLKLHNDQKSDLPTLEVLDDGPGVSEQHLSKMFEP
FFTTESKGTGLGLYLSRELCESNQAHLEYAPRPTGGSCLRITFAHPFKTS

Sequences:

>Translated_530_residues
MTADSPSLVSPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANPNLFRSGCWLYLVFNILVVVLLERPSRQAQFF
GLAMTDAIMLSALFYAAGGPSSGIGNLLIASVAISNVLLRGRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLG
ALCFAAALLVQALTARMQVSEVLAEQRAADVDSLEALNALILQRMRTGILVLDANHQVLLANQGALHLLGQEQLVGQIID
TCSPQLVERLRQWLVNPIMVPRSITTAPVGLVLQPSFATLNRGDQRQTLVFLEDLSQIAHQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLNESEELSAADRRLGQIIHEQSGRIDRVIENVLQLSRRREAQPELLDLKTWLEHFVAEFRSSAAPN
QKIHVDISPGTLTTRMDSGQLTQIMTNLVQNGLRYSGKNHPSAQVWLKLHNDQKSDLPTLEVLDDGPGVSEQHLSKMFEP
FFTTESKGTGLGLYLSRELCESNQAHLEYAPRPTGGSCLRITFAHPFKTS
>Mature_529_residues
TADSPSLVSPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANPNLFRSGCWLYLVFNILVVVLLERPSRQAQFFG
LAMTDAIMLSALFYAAGGPSSGIGNLLIASVAISNVLLRGRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLGA
LCFAAALLVQALTARMQVSEVLAEQRAADVDSLEALNALILQRMRTGILVLDANHQVLLANQGALHLLGQEQLVGQIIDT
CSPQLVERLRQWLVNPIMVPRSITTAPVGLVLQPSFATLNRGDQRQTLVFLEDLSQIAHQAQQLKLAALGRLTAGIAHEI
RNPLGAVSHAAQLLNESEELSAADRRLGQIIHEQSGRIDRVIENVLQLSRRREAQPELLDLKTWLEHFVAEFRSSAAPNQ
KIHVDISPGTLTTRMDSGQLTQIMTNLVQNGLRYSGKNHPSAQVWLKLHNDQKSDLPTLEVLDDGPGVSEQHLSKMFEPF
FTTESKGTGLGLYLSRELCESNQAHLEYAPRPTGGSCLRITFAHPFKTS

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=216, Percent_Identity=27.7777777777778, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1788549, Length=344, Percent_Identity=27.0348837209302, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789808, Length=234, Percent_Identity=25.2136752136752, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790300, Length=346, Percent_Identity=26.3005780346821, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790346, Length=234, Percent_Identity=28.2051282051282, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787894, Length=242, Percent_Identity=26.4462809917355, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788393, Length=247, Percent_Identity=27.5303643724696, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786912, Length=249, Percent_Identity=25.3012048192771, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=238, Percent_Identity=24.3697478991597, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1790861, Length=192, Percent_Identity=27.0833333333333, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1786600, Length=253, Percent_Identity=23.3201581027668, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI87081816, Length=234, Percent_Identity=27.7777777777778, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57988; Mature: 57857

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADSPSLVSPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANPNLFRSGCWLYL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHH
VFNILVVVLLERPSRQAQFFGLAMTDAIMLSALFYAAGGPSSGIGNLLIASVAISNVLLR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
GRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLGALCFAAALLVQALTARMQVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHH
EVLAEQRAADVDSLEALNALILQRMRTGILVLDANHQVLLANQGALHLLGQEQLVGQIID
HHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCCEEECCHHHHHHHHHH
TCSPQLVERLRQWLVNPIMVPRSITTAPVGLVLQPSFATLNRGDQRQTLVFLEDLSQIAH
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHCCCCCCHHHHHHHHHHHHHHH
QAQQLKLAALGRLTAGIAHEIRNPLGAVSHAAQLLNESEELSAADRRLGQIIHEQSGRID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHH
RVIENVLQLSRRREAQPELLDLKTWLEHFVAEFRSSAAPNQKIHVDISPGTLTTRMDSGQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCH
LTQIMTNLVQNGLRYSGKNHPSAQVWLKLHNDQKSDLPTLEVLDDGPGVSEQHLSKMFEP
HHHHHHHHHHCCHHCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHH
FFTTESKGTGLGLYLSRELCESNQAHLEYAPRPTGGSCLRITFAHPFKTS
HHCCCCCCCCEEHHEEHHHHCCCCCCEEECCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
TADSPSLVSPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANPNLFRSGCWLYL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHH
VFNILVVVLLERPSRQAQFFGLAMTDAIMLSALFYAAGGPSSGIGNLLIASVAISNVLLR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
GRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLGALCFAAALLVQALTARMQVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHH
EVLAEQRAADVDSLEALNALILQRMRTGILVLDANHQVLLANQGALHLLGQEQLVGQIID
HHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCCEEECCHHHHHHHHHH
TCSPQLVERLRQWLVNPIMVPRSITTAPVGLVLQPSFATLNRGDQRQTLVFLEDLSQIAH
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHCCCCCCHHHHHHHHHHHHHHH
QAQQLKLAALGRLTAGIAHEIRNPLGAVSHAAQLLNESEELSAADRRLGQIIHEQSGRID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHH
RVIENVLQLSRRREAQPELLDLKTWLEHFVAEFRSSAAPNQKIHVDISPGTLTTRMDSGQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCH
LTQIMTNLVQNGLRYSGKNHPSAQVWLKLHNDQKSDLPTLEVLDDGPGVSEQHLSKMFEP
HHHHHHHHHHCCHHCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHH
FFTTESKGTGLGLYLSRELCESNQAHLEYAPRPTGGSCLRITFAHPFKTS
HHCCCCCCCCEEHHEEHHHHCCCCCCEEECCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]