Click here to switch to the map view.

The map label for this gene is pilR [H]

Identifier: 66043987

GI number: 66043987

Start: 826013

End: 827350

Strand: Reverse

Name: pilR [H]

Synonym: Psyr_0722

Alternate gene names: 66043987

Gene position: 827350-826013 (Counterclockwise)

Preceding gene: 66043988

Following gene: 66043979

Centisome position: 13.58

GC content: 61.58

Gene sequence:

>1338_bases
ATGAGCCAACGGCAAAAAATCCTGATAGTCGATGATGAGCCGGATATTCGCGAACTCCTGGAGATCACGCTGGGACGCAT
GAAACTCGACACCCGTAGTGCGCGTAACGTTGCCGAAGCCCACGACTGGCTGGCCCGCGAACCGTTCGACATGTGCCTGA
CCGACATGCGTCTGCCGGACGGCAATGGCCTTGAGCTGGTGCAACACATACAGCACGGCTACCCCCACGTGCCGGTGGCG
ATGATCACTGCCCACGGCAACCTGGACACCGCGATCCACGCGCTGAAGGCCGGAGCTTTCGACTTTGTGACCAAGCCGGT
CGACCTGGGCAGGTTGCGCGAGCTGGTCAATAGCGCGCTGAGCCTGCCCGGCGCGCAACCCACGCGCAGCATCGACAACC
GCCTGCTGGGCGACTCGCTGGCAATGCGTACCTTGCGCAGTCAGATCGGCAAGCTGGCCCGCAGCCAGGCGCCGATCTAC
ATCAGCGGCGAATCAGGCAGTGGCAAGGAGCTCGTAGCGCGTCTGATCCACGAGCAGGGCCCGCGCATCGACAAACCTTT
CATACCGGTGAATTGCGGCGCCATTCCTTCCGAACTCATGGAAAGCGAATTCTTCGGTCATCGCAAAGGCAGCTTTAGCG
GTGCCCACGAAGACAAGCCAGGTCTGTTTCAGGCGGCGCATACCGGGACGCTGTTTCTCGATGAGGTCGCCGACCTGCCG
CTGGCCATGCAGGTCAAACTGCTGCGCGCCATTCAGGAAAAATCCATCCGCAGCGTCGGCGGCCAACAAGAGCAGATCGT
CGACGTGCGCATTCTGTGCGCCACCCACAAGAACCTGAATGCAGAGGTCGCGGCCGGTCGGTTCCGTCAGGATCTGTATT
ACCGATTGAACGTGATCGAAGTGCGCGTGCCCTCGTTGCGCGAGCGGCGTGAGGATATCGACCAACTGGCAGCCAGCGTG
CTCAAGCGCCTTGCCGGGAACGGCGCACAACCCGTCGCGCGCCTGAACGCGCAGGCGCTGGAAACGCTGAAGAGCTACCG
CTTCCCCGGTAACGTGCGCGAACTGGAAAACATGCTGGAGCGCGCTTACACGCTGTGCGAGAACGACGAAATCCACGCCA
GCGACCTGCGCCTGACAGAGTCTGCCAGCCCTCAGGAGAACGACGGCCCGAGCCTGGCCGATATCGACAACCTTGAAGAC
TACCTGGAAAGCGTCGAACGCCAGCTCATTCTGCAGGCGCTGGAAGAAACCCGCTGGAACCGCACCGCTGCGGCAGAAAG
GCTCAGTTTGTCGTTCAGGTCACTGCGCTATCGCCTGAAGAAGCTGGGCCTGGATTGA

Upstream 100 bases:

>100_bases
TGTGTGAAAGCAATCAGGCGCATCTGGAATACGCGCCCCGCCCCACGGGTGGCAGTTGCCTGCGCATTACCTTCGCCCAC
CCGTTCAAGACGAGCTGAGC

Downstream 100 bases:

>100_bases
ATGCGTTAAGCGGCAGCAACTATGTGACGCAGAGTGTCACGAAAGGCGTTCCCACGCTGGAGCGTGCGGACGATAGGTGC
GCGTGAGAACACCCTCGTGC

Product: helix-turn-helix, Fis-type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSQRQKILIVDDEPDIRELLEITLGRMKLDTRSARNVAEAHDWLAREPFDMCLTDMRLPDGNGLELVQHIQHGYPHVPVA
MITAHGNLDTAIHALKAGAFDFVTKPVDLGRLRELVNSALSLPGAQPTRSIDNRLLGDSLAMRTLRSQIGKLARSQAPIY
ISGESGSGKELVARLIHEQGPRIDKPFIPVNCGAIPSELMESEFFGHRKGSFSGAHEDKPGLFQAAHTGTLFLDEVADLP
LAMQVKLLRAIQEKSIRSVGGQQEQIVDVRILCATHKNLNAEVAAGRFRQDLYYRLNVIEVRVPSLRERREDIDQLAASV
LKRLAGNGAQPVARLNAQALETLKSYRFPGNVRELENMLERAYTLCENDEIHASDLRLTESASPQENDGPSLADIDNLED
YLESVERQLILQALEETRWNRTAAAERLSLSFRSLRYRLKKLGLD

Sequences:

>Translated_445_residues
MSQRQKILIVDDEPDIRELLEITLGRMKLDTRSARNVAEAHDWLAREPFDMCLTDMRLPDGNGLELVQHIQHGYPHVPVA
MITAHGNLDTAIHALKAGAFDFVTKPVDLGRLRELVNSALSLPGAQPTRSIDNRLLGDSLAMRTLRSQIGKLARSQAPIY
ISGESGSGKELVARLIHEQGPRIDKPFIPVNCGAIPSELMESEFFGHRKGSFSGAHEDKPGLFQAAHTGTLFLDEVADLP
LAMQVKLLRAIQEKSIRSVGGQQEQIVDVRILCATHKNLNAEVAAGRFRQDLYYRLNVIEVRVPSLRERREDIDQLAASV
LKRLAGNGAQPVARLNAQALETLKSYRFPGNVRELENMLERAYTLCENDEIHASDLRLTESASPQENDGPSLADIDNLED
YLESVERQLILQALEETRWNRTAAAERLSLSFRSLRYRLKKLGLD
>Mature_444_residues
SQRQKILIVDDEPDIRELLEITLGRMKLDTRSARNVAEAHDWLAREPFDMCLTDMRLPDGNGLELVQHIQHGYPHVPVAM
ITAHGNLDTAIHALKAGAFDFVTKPVDLGRLRELVNSALSLPGAQPTRSIDNRLLGDSLAMRTLRSQIGKLARSQAPIYI
SGESGSGKELVARLIHEQGPRIDKPFIPVNCGAIPSELMESEFFGHRKGSFSGAHEDKPGLFQAAHTGTLFLDEVADLPL
AMQVKLLRAIQEKSIRSVGGQQEQIVDVRILCATHKNLNAEVAAGRFRQDLYYRLNVIEVRVPSLRERREDIDQLAASVL
KRLAGNGAQPVARLNAQALETLKSYRFPGNVRELENMLERAYTLCENDEIHASDLRLTESASPQENDGPSLADIDNLEDY
LESVERQLILQALEETRWNRTAAAERLSLSFRSLRYRLKKLGLD

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=458, Percent_Identity=39.3013100436681, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1790437, Length=445, Percent_Identity=40.8988764044944, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=38.034188034188, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1788905, Length=434, Percent_Identity=38.0184331797235, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=40.4984423676012, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082117, Length=323, Percent_Identity=40.2476780185758, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI87082152, Length=331, Percent_Identity=40.4833836858006, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1789087, Length=375, Percent_Identity=37.3333333333333, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1786524, Length=353, Percent_Identity=39.3767705382436, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87081872, Length=317, Percent_Identity=35.9621451104101, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1787583, Length=312, Percent_Identity=35.5769230769231, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1789828, Length=234, Percent_Identity=35.042735042735, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081858, Length=390, Percent_Identity=27.1794871794872, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1789809, Length=130, Percent_Identity=33.8461538461538, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787487, Length=117, Percent_Identity=35.8974358974359, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49738; Mature: 49607

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRQKILIVDDEPDIRELLEITLGRMKLDTRSARNVAEAHDWLAREPFDMCLTDMRLPD
CCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
GNGLELVQHIQHGYPHVPVAMITAHGNLDTAIHALKAGAFDFVTKPVDLGRLRELVNSAL
CCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHH
SLPGAQPTRSIDNRLLGDSLAMRTLRSQIGKLARSQAPIYISGESGSGKELVARLIHEQG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCC
PRIDKPFIPVNCGAIPSELMESEFFGHRKGSFSGAHEDKPGLFQAAHTGTLFLDEVADLP
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHCCCCCHHHHHHCCC
LAMQVKLLRAIQEKSIRSVGGQQEQIVDVRILCATHKNLNAEVAAGRFRQDLYYRLNVIE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHEEEEEE
VRVPSLRERREDIDQLAASVLKRLAGNGAQPVARLNAQALETLKSYRFPGNVRELENMLE
EECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RAYTLCENDEIHASDLRLTESASPQENDGPSLADIDNLEDYLESVERQLILQALEETRWN
HHHHHHCCCCCCHHHCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
RTAAAERLSLSFRSLRYRLKKLGLD
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQRQKILIVDDEPDIRELLEITLGRMKLDTRSARNVAEAHDWLAREPFDMCLTDMRLPD
CCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
GNGLELVQHIQHGYPHVPVAMITAHGNLDTAIHALKAGAFDFVTKPVDLGRLRELVNSAL
CCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHH
SLPGAQPTRSIDNRLLGDSLAMRTLRSQIGKLARSQAPIYISGESGSGKELVARLIHEQG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCC
PRIDKPFIPVNCGAIPSELMESEFFGHRKGSFSGAHEDKPGLFQAAHTGTLFLDEVADLP
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHCCCCCHHHHHHCCC
LAMQVKLLRAIQEKSIRSVGGQQEQIVDVRILCATHKNLNAEVAAGRFRQDLYYRLNVIE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHEEEEEE
VRVPSLRERREDIDQLAASVLKRLAGNGAQPVARLNAQALETLKSYRFPGNVRELENMLE
EECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RAYTLCENDEIHASDLRLTESASPQENDGPSLADIDNLEDYLESVERQLILQALEETRWN
HHHHHHCCCCCCHHHCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
RTAAAERLSLSFRSLRYRLKKLGLD
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]