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The map label for this gene is yfmE [H]

Identifier: 66043932

GI number: 66043932

Start: 753604

End: 754572

Strand: Reverse

Name: yfmE [H]

Synonym: Psyr_0665

Alternate gene names: 66043932

Gene position: 754572-753604 (Counterclockwise)

Preceding gene: 161486738

Following gene: 66043931

Centisome position: 12.38

GC content: 66.36

Gene sequence:

>969_bases
ATGAACCACCCGCGCGCACGGCTGTTGCTGCTGATGACCCTGCTGGCACTCGCCCTGCTGCTGGGCCTCAGCGCTGGCAC
CGTGTGGCTGACACCCGGTGCCGTACTCGATCACCTGATCGCCCACGACAGCAAGGACTTCGAAGTCTGGAATCACCGCC
TGCCACGCGGCCTGATCGCGATCATGACCGGCTGCGCGTTCGGCCTGGCTGGCGCCATCGTGCAAGGCGTGATTCGCAAC
CCGCTGGCCTCGCCGGAAATCCTCGGCGTCACTCAGGGCGCCGGGCTGGCGCTGACCATCGCGATTATCGGCCTGCCGCA
GTTACCGGTGTCGGCGCTGCCGTTTGTGGCGTGTCTGGGCGGCGCGGCGGGAGCGCTGCTGCTGGCGTTGTACAACACCG
GCGTGCAGTTTTCCGGCGTGCGTTTCGCGTTGTCCGGGGTCGCGATTGCAGTGACGTTGTCGAGCATCACCGAGTTCCTG
ATCCTGTCCAACCCGCTGGATATCAACACCGCCCTGCTGGCGCTGACCGGCAGCCTGTGGAGCCGCAACTGGCACCACGT
CTTGCTGGCCACGCCGCTTCTGCTGCTGATCCCGCTCAGCCTGCTGCTGGCCAAACCGCTGAACCTGATCGGCCTTGGCG
ATGAGGCCGCGCAGAGCCTGGGCACCGGCCTCACGCGTACGCGCTGGCTGGCGATGGGCAGTGCCGTGCTGTTGACCAGC
CTGGGGGTGGGCATTATCGGCCCGGTGAGCTTCGTCGGCCTGGTCGCACCACACATGGCCCGCCGTCTGGTCGGCGGTCA
TCATCAGTACCTGCTGCCCGCCTCGATGGTGCTGGGCGCGTTATTGCTGGTGCTGGCCGACACGCTGGGACGCACATTGA
TCGCCCCCAGCGAAATTCCGGCCGGGATTCTCACGGCGGTGATCGGCGCTCCGTATTTCCTCTGGCTGCTGGCCCGATTC
AAAGGTTGA

Upstream 100 bases:

>100_bases
TGATCAGCCGCGCCGTGGCCTTCCCGGTGGAAACACCGGCCGGGGTGGTGACGGCATTGATCGGCGCGCCGTTCTTTCTG
TTTCTAGCCAGGCGTGCGCT

Downstream 100 bases:

>100_bases
TCCCATGACTATTCTGAAGGCGCAGCAACTCGACATCGGCTACGGCGCAACGCGCATCGTGCAAGACCTGTCGTTCAGCC
CGCCGCCTGCGCAGGTCACT

Product: transport system permease protein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: Ferric-citrate import system permease protein [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNHPRARLLLLMTLLALALLLGLSAGTVWLTPGAVLDHLIAHDSKDFEVWNHRLPRGLIAIMTGCAFGLAGAIVQGVIRN
PLASPEILGVTQGAGLALTIAIIGLPQLPVSALPFVACLGGAAGALLLALYNTGVQFSGVRFALSGVAIAVTLSSITEFL
ILSNPLDINTALLALTGSLWSRNWHHVLLATPLLLLIPLSLLLAKPLNLIGLGDEAAQSLGTGLTRTRWLAMGSAVLLTS
LGVGIIGPVSFVGLVAPHMARRLVGGHHQYLLPASMVLGALLLVLADTLGRTLIAPSEIPAGILTAVIGAPYFLWLLARF
KG

Sequences:

>Translated_322_residues
MNHPRARLLLLMTLLALALLLGLSAGTVWLTPGAVLDHLIAHDSKDFEVWNHRLPRGLIAIMTGCAFGLAGAIVQGVIRN
PLASPEILGVTQGAGLALTIAIIGLPQLPVSALPFVACLGGAAGALLLALYNTGVQFSGVRFALSGVAIAVTLSSITEFL
ILSNPLDINTALLALTGSLWSRNWHHVLLATPLLLLIPLSLLLAKPLNLIGLGDEAAQSLGTGLTRTRWLAMGSAVLLTS
LGVGIIGPVSFVGLVAPHMARRLVGGHHQYLLPASMVLGALLLVLADTLGRTLIAPSEIPAGILTAVIGAPYFLWLLARF
KG
>Mature_322_residues
MNHPRARLLLLMTLLALALLLGLSAGTVWLTPGAVLDHLIAHDSKDFEVWNHRLPRGLIAIMTGCAFGLAGAIVQGVIRN
PLASPEILGVTQGAGLALTIAIIGLPQLPVSALPFVACLGGAAGALLLALYNTGVQFSGVRFALSGVAIAVTLSSITEFL
ILSNPLDINTALLALTGSLWSRNWHHVLLATPLLLLIPLSLLLAKPLNLIGLGDEAAQSLGTGLTRTRWLAMGSAVLLTS
LGVGIIGPVSFVGLVAPHMARRLVGGHHQYLLPASMVLGALLLVLADTLGRTLIAPSEIPAGILTAVIGAPYFLWLLARF
KG

Specific function: Part of the ABC transporter complex yfmCDEF involved in citrate-dependent Fe(3+) import. Involved in the translocation of the substrate across the membrane (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790740, Length=303, Percent_Identity=46.8646864686469, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1790741, Length=276, Percent_Identity=36.9565217391304, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786805, Length=277, Percent_Identity=36.8231046931408, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788004, Length=301, Percent_Identity=33.8870431893688, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786347, Length=279, Percent_Identity=32.9749103942652, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1786804, Length=313, Percent_Identity=33.8658146964856, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000522 [H]

Pfam domain/function: PF01032 FecCD [H]

EC number: NA

Molecular weight: Translated: 33463; Mature: 33463

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHPRARLLLLMTLLALALLLGLSAGTVWLTPGAVLDHLIAHDSKDFEVWNHRLPRGLIA
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHCCHHHHH
IMTGCAFGLAGAIVQGVIRNPLASPEILGVTQGAGLALTIAIIGLPQLPVSALPFVACLG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEHEECCCCCCCHHHHHHHHHHH
GAAGALLLALYNTGVQFSGVRFALSGVAIAVTLSSITEFLILSNPLDINTALLALTGSLW
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SRNWHHVLLATPLLLLIPLSLLLAKPLNLIGLGDEAAQSLGTGLTRTRWLAMGSAVLLTS
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHH
LGVGIIGPVSFVGLVAPHMARRLVGGHHQYLLPASMVLGALLLVLADTLGRTLIAPSEIP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCC
AGILTAVIGAPYFLWLLARFKG
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNHPRARLLLLMTLLALALLLGLSAGTVWLTPGAVLDHLIAHDSKDFEVWNHRLPRGLIA
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHCCHHHHH
IMTGCAFGLAGAIVQGVIRNPLASPEILGVTQGAGLALTIAIIGLPQLPVSALPFVACLG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEHEECCCCCCCHHHHHHHHHHH
GAAGALLLALYNTGVQFSGVRFALSGVAIAVTLSSITEFLILSNPLDINTALLALTGSLW
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SRNWHHVLLATPLLLLIPLSLLLAKPLNLIGLGDEAAQSLGTGLTRTRWLAMGSAVLLTS
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHH
LGVGIIGPVSFVGLVAPHMARRLVGGHHQYLLPASMVLGALLLVLADTLGRTLIAPSEIP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCC
AGILTAVIGAPYFLWLLARFKG
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]