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The map label for this gene is tolC [H]

Identifier: 66043810

GI number: 66043810

Start: 613307

End: 614746

Strand: Reverse

Name: tolC [H]

Synonym: Psyr_0543

Alternate gene names: 66043810

Gene position: 614746-613307 (Counterclockwise)

Preceding gene: 66043813

Following gene: 66043808

Centisome position: 10.09

GC content: 63.06

Gene sequence:

>1440_bases
ATGTTGCGCAAACTTTCGTTGGTCGTGGCTGTTTCGTTTGCGTCCAGCGGGTTGACCTGGGCAGCCGAATTGCCGATGCC
AACGCAGACAGGGCTGTTGAGCGTTTATCAGCAGGCCGTGGACAACAACGCCGACCTCGCGGCCTCGCGCGCCGATTACG
ATGCGCGCAAGGAAGCCGTGCCGCAGGCCCGCGCCGGTTTGCTGCCGAACATTTCCGGCAGCGCCCAGAACAGCAGCACC
CGCACCAGCATCGATCAGCCCAGAGCCGTGGCCAGCCGCAGCGGCAACCTCTACCAGGCCACGCTGAGCCAGCCGATCTT
CCGCGCCGACCGCTGGTTCCAGTTGCAGGCTGCCGAAGCGAGCAACGAACAGGCGGCACTGGAGCTGTCGGCTACCGAGC
AGAATCTGATCCTGCAATCGGCACAGAGCTATTTCAGCGTACTGCGTGCACAGGACAACCTGGCTTCGACCAAGGCCGAG
GAAGCGGCCTTCAAGCGCCAGCTGGATCAGGCCAACGAGCGCTTCGATGTCGGCCTGTCGGACAAGACCGATGTGCTGCA
GGCGCAGGCCAGCTATGACACGTCACGCGCCAGCCGACTCATTGCCCAACGTCAGGTCGAGGATGCCTTTCAGGCGCTGG
TGACGCTGACCAACCGCGAATACAACTCGATCGAAGGCATCGTGCATACCCTGCCGGTGCTGGCGCCGACGCCCAACGAT
GCCAAGGCCTGGGTCGATACTGCCGCGCAGCAGAACCTCAACCTGCTGGCCAGCAACTACGCGGTCAATGCCGCCGAAGA
AACCCTGCGCCAGCGCAAGGCCGGACACGCGCCGACGCTCGATGCCGTGGCGACCTATCAGCGTGGCGATAACGATGCAC
TGGGTTTCAGCAACCCCAACTACACCGGTCGCAATTACGGCGGCGACGTCGAGCAGCGCAGCATTGGCGTGCAGTTGAAC
ATCCCGATCTACAGCGGCGGCCTGACCAGCTCGCAGGTACGCGAAGCCTATGCGCGCCTGAGCCAGAGCGAGCAGCGGCG
TGAAAGCCTGCGTCGTCAGGTGGTGGAAAACACCCGCAACCTGCATCGCGCGGTGAATACCGACGTGGAGCAGGTCCAGG
CCCGCAAGCAGTCGATCATTTCCAACCAGAGCGCGCTGGAAGCCACAGAAATCGGTTATCAGGTCGGCACCCGCAACATC
GTCGACGTGCTGGATGCGCAACGCCAGCTGTATGCCTCGGTGCGTGACTACAACAACACGCGTTACGACTACATCCTCGA
CAACCTGCGCCTCAAGCAGGCAGCAGGCACTCTGAACCCGGGCGACCTGCAGGACCTGTCGCGCTACCTGAAGGCCGACT
ACAACCCGGACAAGGACTTCCTGCCGCCGGACCTGGCGACTGCTGCGCAGAAGAACTTCGAACGGCCTGCAAAGCGCTGA

Upstream 100 bases:

>100_bases
ATCGACGATTGTTGCCAAAAGCGTCTGGCTCTACACTCCCTAGCCCGGTTTCTCTTCAACCCGAACTGTAATTATTTTCT
TCATGAACAGGGAATGCCTC

Downstream 100 bases:

>100_bases
GATTGCTGACCGCTCTGGATGCTCTGCGCCCGACTTCAAGTCATGCAGCGCGACGCAGATTTGTGACGCGGAGCGTCACC
CAAGGCATTCCCACGCTGGA

Product: Type I secretion outer membrane protein, TolC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MLRKLSLVVAVSFASSGLTWAAELPMPTQTGLLSVYQQAVDNNADLAASRADYDARKEAVPQARAGLLPNISGSAQNSST
RTSIDQPRAVASRSGNLYQATLSQPIFRADRWFQLQAAEASNEQAALELSATEQNLILQSAQSYFSVLRAQDNLASTKAE
EAAFKRQLDQANERFDVGLSDKTDVLQAQASYDTSRASRLIAQRQVEDAFQALVTLTNREYNSIEGIVHTLPVLAPTPND
AKAWVDTAAQQNLNLLASNYAVNAAEETLRQRKAGHAPTLDAVATYQRGDNDALGFSNPNYTGRNYGGDVEQRSIGVQLN
IPIYSGGLTSSQVREAYARLSQSEQRRESLRRQVVENTRNLHRAVNTDVEQVQARKQSIISNQSALEATEIGYQVGTRNI
VDVLDAQRQLYASVRDYNNTRYDYILDNLRLKQAAGTLNPGDLQDLSRYLKADYNPDKDFLPPDLATAAQKNFERPAKR

Sequences:

>Translated_479_residues
MLRKLSLVVAVSFASSGLTWAAELPMPTQTGLLSVYQQAVDNNADLAASRADYDARKEAVPQARAGLLPNISGSAQNSST
RTSIDQPRAVASRSGNLYQATLSQPIFRADRWFQLQAAEASNEQAALELSATEQNLILQSAQSYFSVLRAQDNLASTKAE
EAAFKRQLDQANERFDVGLSDKTDVLQAQASYDTSRASRLIAQRQVEDAFQALVTLTNREYNSIEGIVHTLPVLAPTPND
AKAWVDTAAQQNLNLLASNYAVNAAEETLRQRKAGHAPTLDAVATYQRGDNDALGFSNPNYTGRNYGGDVEQRSIGVQLN
IPIYSGGLTSSQVREAYARLSQSEQRRESLRRQVVENTRNLHRAVNTDVEQVQARKQSIISNQSALEATEIGYQVGTRNI
VDVLDAQRQLYASVRDYNNTRYDYILDNLRLKQAAGTLNPGDLQDLSRYLKADYNPDKDFLPPDLATAAQKNFERPAKR
>Mature_479_residues
MLRKLSLVVAVSFASSGLTWAAELPMPTQTGLLSVYQQAVDNNADLAASRADYDARKEAVPQARAGLLPNISGSAQNSST
RTSIDQPRAVASRSGNLYQATLSQPIFRADRWFQLQAAEASNEQAALELSATEQNLILQSAQSYFSVLRAQDNLASTKAE
EAAFKRQLDQANERFDVGLSDKTDVLQAQASYDTSRASRLIAQRQVEDAFQALVTLTNREYNSIEGIVHTLPVLAPTPND
AKAWVDTAAQQNLNLLASNYAVNAAEETLRQRKAGHAPTLDAVATYQRGDNDALGFSNPNYTGRNYGGDVEQRSIGVQLN
IPIYSGGLTSSQVREAYARLSQSEQRRESLRRQVVENTRNLHRAVNTDVEQVQARKQSIISNQSALEATEIGYQVGTRNI
VDVLDAQRQLYASVRDYNNTRYDYILDNLRLKQAAGTLNPGDLQDLSRYLKADYNPDKDFLPPDLATAAQKNFERPAKR

Specific function: May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=434, Percent_Identity=34.5622119815668, Blast_Score=223, Evalue=2e-59,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52834; Mature: 52834

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKLSLVVAVSFASSGLTWAAELPMPTQTGLLSVYQQAVDNNADLAASRADYDARKEAV
CCCCEEEHEEEEHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHC
PQARAGLLPNISGSAQNSSTRTSIDQPRAVASRSGNLYQATLSQPIFRADRWFQLQAAEA
CHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEHHHHCHHHHCCCEEEEEECCC
SNEQAALELSATEQNLILQSAQSYFSVLRAQDNLASTKAEEAAFKRQLDQANERFDVGLS
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DKTDVLQAQASYDTSRASRLIAQRQVEDAFQALVTLTNREYNSIEGIVHTLPVLAPTPND
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
AKAWVDTAAQQNLNLLASNYAVNAAEETLRQRKAGHAPTLDAVATYQRGDNDALGFSNPN
HHHHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCC
YTGRNYGGDVEQRSIGVQLNIPIYSGGLTSSQVREAYARLSQSEQRRESLRRQVVENTRN
CCCCCCCCCCHHHEECEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHRAVNTDVEQVQARKQSIISNQSALEATEIGYQVGTRNIVDVLDAQRQLYASVRDYNNT
HHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
RYDYILDNLRLKQAAGTLNPGDLQDLSRYLKADYNPDKDFLPPDLATAAQKNFERPAKR
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLRKLSLVVAVSFASSGLTWAAELPMPTQTGLLSVYQQAVDNNADLAASRADYDARKEAV
CCCCEEEHEEEEHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHC
PQARAGLLPNISGSAQNSSTRTSIDQPRAVASRSGNLYQATLSQPIFRADRWFQLQAAEA
CHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEHHHHCHHHHCCCEEEEEECCC
SNEQAALELSATEQNLILQSAQSYFSVLRAQDNLASTKAEEAAFKRQLDQANERFDVGLS
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DKTDVLQAQASYDTSRASRLIAQRQVEDAFQALVTLTNREYNSIEGIVHTLPVLAPTPND
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
AKAWVDTAAQQNLNLLASNYAVNAAEETLRQRKAGHAPTLDAVATYQRGDNDALGFSNPN
HHHHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCC
YTGRNYGGDVEQRSIGVQLNIPIYSGGLTSSQVREAYARLSQSEQRRESLRRQVVENTRN
CCCCCCCCCCHHHEECEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHRAVNTDVEQVQARKQSIISNQSALEATEIGYQVGTRNIVDVLDAQRQLYASVRDYNNT
HHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
RYDYILDNLRLKQAAGTLNPGDLQDLSRYLKADYNPDKDFLPPDLATAAQKNFERPAKR
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]