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The map label for this gene is aceF [H]

Identifier: 66043784

GI number: 66043784

Start: 574465

End: 576138

Strand: Reverse

Name: aceF [H]

Synonym: Psyr_0517

Alternate gene names: 66043784

Gene position: 576138-574465 (Counterclockwise)

Preceding gene: 66043785

Following gene: 66043783

Centisome position: 9.45

GC content: 63.8

Gene sequence:

>1674_bases
GTGAGTGAGTTAATTCGTGTACCCGACATCGGCAACGGTGAAGGCGAAGTCATTGAATTGATGGTCAAGGTCGGTGATCG
CATCGAAGCCGACCAGAGCGTTCTGACGCTTGAGTCGGACAAGGCGAGCATGGAAATCCCTGCTCCCAAGGCTGGCGTCA
TCAAGACCATGAAGGTCAAGCTGGGTGACCGCCTGAAAGAAGGCGACGAGCTGTTCGAACTGGAAGTGGAAGGCGAAGCG
GCTGCGGCTGCGGCCCCTGCTGCCGAACCTGCCCCTGCCCCTGCCCCTGCCCCTGCCCCTGCTGCTGCCGAAAAGCCGGC
TGAAGCGGCACCTGCACCTGCGGCCGCCGCCGAGCCTGCTCCGGCAGCCAGCGCCAGCGTTCAGGACATCCACGTACCGG
ACATCGGTTCGTCGGGCAAGGCCAAGATCATCGAACTGATGGTCAAGGTCGGTGACAGCATCCAGGCCGATCAGTCGCTG
ATCACCCTTGAATCCGACAAGGCCAGCATGGAGATCCCTTCTCCGGCAGCAGGCGTTGTCGAGAGCATCGAAGTCAAGCT
GGACCAGGAAGTCGGTACCGGCGACCTGATCCTGAAACTGAAAGTCGAAGGCGCCGCGCCTGCCGCTGCTCCGGCGCCTG
CCGCCAGCGCACCCGCCGCGGCCCCGGCCAAGGCTGAGGCCGCGGCTCCAGCTGCCCCTGCTCCCAAAGCAGAAGCAGCA
CCTGCGCCAGCGGCTCCAGCGGCTCCGGCCAAAGACGGTGCCAAGGTTCATGCCGGCCCGGCCGTTCGTCAGCTGGCCCG
CGAATTTGGCGTCGAGCTGAACGCTGTCAGCGCCACCGGCCCGCACGGCCGTGTGCTGAAAGAAGACGTTCAGGCTTACG
TCAAAACCATGATGCAGAAGGCCAAGGAAGCTCCAGCTGGCGGCGCAAGCGGCGGCTCGGGCATCCCGCCGATTCCGGAA
GTCGATTTCAGCCGTTTCGGCGAGATCGAAGAAGTACCGATGACGCGCCTGATGCAACTCGGCGCTTCCGGCCTGCACCG
CAGCTGGCTGAACATTCCGCACGTCACGCAGTTCGACCAGGCCGACATTACCGACCTGGAAGCTTTCCGTGTGGCGCAGA
AAGGCGCTGCCGAGAAGGCTGGCGTCAAGCTGACGGTCCTGCCGCTGCTGCTCAAATCCTGCGCCCACCTGCTCAAGGAG
CTGCCGGACTTCAACGCTTCGCTGGCGCCTAGCGGCAAGGCCGTGATTCGCAAGAAGTACGTGCACATCGGCTTTGCCGT
GGACACCCCGGACGGCCTGCTGGTCCCGGTCATCCGCGACGTCGACCAGAAGAGCCTGTTGCAACTGGCCGCCGAAGCCG
CCGCACTGGCGGAAAAGGCGCGCAACAAGAAACTGACGGCGAACGACATGCAGGGCGCGTGCTTCACCATCTCCAGCCTC
GGCCACATTGGCGGCACCGGCTTCACGCCGATCGTCAACGCGCCGGAAGTGGCGATCCTCGGTGTTTCCAAGGCAACCAT
CCAGCCCGTCTGGGACGGCAAAGCCTTCCAGCCGAAACTGATGCTGCCGCTGTCGCTGTCCTATGATCACCGCGTGATCA
ACGGCGCAGCTGCTGCCCGCTTCACCAAGCGCTTGAGCGAATTGCTGGCGGACATCCGCACAATCCTGCTGTAA

Upstream 100 bases:

>100_bases
GATGCGGAATTTACTCGACGTAAATGAGCATCCAACGCTGTTTCGTTGTAACTGAACCAGCGTATTCGTGAGTGAACAGG
TTCTCCTAAGGAGAGACATA

Downstream 100 bases:

>100_bases
GCCCTGCCGGCCCGTCCCCGGACGGGCCTTGCTGTACACCCTTGCGAGCTGCCACGCTCGAACCTCAACCCCGCCCTCCA
GCGGGGATTTTTTTGCCCTG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MSELIRVPDIGNGEGEVIELMVKVGDRIEADQSVLTLESDKASMEIPAPKAGVIKTMKVKLGDRLKEGDELFELEVEGEA
AAAAAPAAEPAPAPAPAPAPAAAEKPAEAAPAPAAAAEPAPAASASVQDIHVPDIGSSGKAKIIELMVKVGDSIQADQSL
ITLESDKASMEIPSPAAGVVESIEVKLDQEVGTGDLILKLKVEGAAPAAAPAPAASAPAAAPAKAEAAAPAAPAPKAEAA
PAPAAPAAPAKDGAKVHAGPAVRQLAREFGVELNAVSATGPHGRVLKEDVQAYVKTMMQKAKEAPAGGASGGSGIPPIPE
VDFSRFGEIEEVPMTRLMQLGASGLHRSWLNIPHVTQFDQADITDLEAFRVAQKGAAEKAGVKLTVLPLLLKSCAHLLKE
LPDFNASLAPSGKAVIRKKYVHIGFAVDTPDGLLVPVIRDVDQKSLLQLAAEAAALAEKARNKKLTANDMQGACFTISSL
GHIGGTGFTPIVNAPEVAILGVSKATIQPVWDGKAFQPKLMLPLSLSYDHRVINGAAAARFTKRLSELLADIRTILL

Sequences:

>Translated_557_residues
MSELIRVPDIGNGEGEVIELMVKVGDRIEADQSVLTLESDKASMEIPAPKAGVIKTMKVKLGDRLKEGDELFELEVEGEA
AAAAAPAAEPAPAPAPAPAPAAAEKPAEAAPAPAAAAEPAPAASASVQDIHVPDIGSSGKAKIIELMVKVGDSIQADQSL
ITLESDKASMEIPSPAAGVVESIEVKLDQEVGTGDLILKLKVEGAAPAAAPAPAASAPAAAPAKAEAAAPAAPAPKAEAA
PAPAAPAAPAKDGAKVHAGPAVRQLAREFGVELNAVSATGPHGRVLKEDVQAYVKTMMQKAKEAPAGGASGGSGIPPIPE
VDFSRFGEIEEVPMTRLMQLGASGLHRSWLNIPHVTQFDQADITDLEAFRVAQKGAAEKAGVKLTVLPLLLKSCAHLLKE
LPDFNASLAPSGKAVIRKKYVHIGFAVDTPDGLLVPVIRDVDQKSLLQLAAEAAALAEKARNKKLTANDMQGACFTISSL
GHIGGTGFTPIVNAPEVAILGVSKATIQPVWDGKAFQPKLMLPLSLSYDHRVINGAAAARFTKRLSELLADIRTILL
>Mature_556_residues
SELIRVPDIGNGEGEVIELMVKVGDRIEADQSVLTLESDKASMEIPAPKAGVIKTMKVKLGDRLKEGDELFELEVEGEAA
AAAAPAAEPAPAPAPAPAPAAAEKPAEAAPAPAAAAEPAPAASASVQDIHVPDIGSSGKAKIIELMVKVGDSIQADQSLI
TLESDKASMEIPSPAAGVVESIEVKLDQEVGTGDLILKLKVEGAAPAAAPAPAASAPAAAPAKAEAAAPAAPAPKAEAAP
APAAPAAPAKDGAKVHAGPAVRQLAREFGVELNAVSATGPHGRVLKEDVQAYVKTMMQKAKEAPAGGASGGSGIPPIPEV
DFSRFGEIEEVPMTRLMQLGASGLHRSWLNIPHVTQFDQADITDLEAFRVAQKGAAEKAGVKLTVLPLLLKSCAHLLKEL
PDFNASLAPSGKAVIRKKYVHIGFAVDTPDGLLVPVIRDVDQKSLLQLAAEAAALAEKARNKKLTANDMQGACFTISSLG
HIGGTGFTPIVNAPEVAILGVSKATIQPVWDGKAFQPKLMLPLSLSYDHRVINGAAAARFTKRLSELLADIRTILL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=474, Percent_Identity=27.6371308016878, Blast_Score=158, Evalue=2e-38,
Organism=Homo sapiens, GI31711992, Length=332, Percent_Identity=31.6265060240964, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=34.6666666666667, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI203098816, Length=435, Percent_Identity=25.9770114942529, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI203098753, Length=435, Percent_Identity=25.9770114942529, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI260898739, Length=151, Percent_Identity=34.4370860927152, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1786305, Length=555, Percent_Identity=49.009009009009, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1786946, Length=431, Percent_Identity=28.538283062645, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=29.9303944315545, Blast_Score=177, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=420, Percent_Identity=30.7142857142857, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=29.6127562642369, Blast_Score=142, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=30.8196721311475, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=450, Percent_Identity=29.1111111111111, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6324258, Length=431, Percent_Identity=27.3781902552204, Blast_Score=126, Evalue=9e-30,
Organism=Drosophila melanogaster, GI18859875, Length=443, Percent_Identity=29.1196388261851, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=32.1888412017167, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=32.1888412017167, Blast_Score=120, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24645909, Length=217, Percent_Identity=33.1797235023041, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 57278; Mature: 57147

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELIRVPDIGNGEGEVIELMVKVGDRIEADQSVLTLESDKASMEIPAPKAGVIKTMKVK
CCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCEEHHHHH
LGDRLKEGDELFELEVEGEAAAAAAPAAEPAPAPAPAPAPAAAEKPAEAAPAPAAAAEPA
HHHHCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAASASVQDIHVPDIGSSGKAKIIELMVKVGDSIQADQSLITLESDKASMEIPSPAAGVV
CCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHH
ESIEVKLDQEVGTGDLILKLKVEGAAPAAAPAPAASAPAAAPAKAEAAAPAAPAPKAEAA
HHHHEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAPAAPAAPAKDGAKVHAGPAVRQLAREFGVELNAVSATGPHGRVLKEDVQAYVKTMMQK
CCCCCCCCCCCCCCEEECCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
AKEAPAGGASGGSGIPPIPEVDFSRFGEIEEVPMTRLMQLGASGLHRSWLNIPHVTQFDQ
HHCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCC
ADITDLEAFRVAQKGAAEKAGVKLTVLPLLLKSCAHLLKELPDFNASLAPSGKAVIRKKY
CCCHHHHHHHHHHHCCHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEE
VHIGFAVDTPDGLLVPVIRDVDQKSLLQLAAEAAALAEKARNKKLTANDMQGACFTISSL
EEEEEEEECCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHH
GHIGGTGFTPIVNAPEVAILGVSKATIQPVWDGKAFQPKLMLPLSLSYDHRVINGAAAAR
HCCCCCCCCCCCCCCCEEEEECCHHEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHH
FTKRLSELLADIRTILL
HHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SELIRVPDIGNGEGEVIELMVKVGDRIEADQSVLTLESDKASMEIPAPKAGVIKTMKVK
CCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCEEHHHHH
LGDRLKEGDELFELEVEGEAAAAAAPAAEPAPAPAPAPAPAAAEKPAEAAPAPAAAAEPA
HHHHCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAASASVQDIHVPDIGSSGKAKIIELMVKVGDSIQADQSLITLESDKASMEIPSPAAGVV
CCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHH
ESIEVKLDQEVGTGDLILKLKVEGAAPAAAPAPAASAPAAAPAKAEAAAPAAPAPKAEAA
HHHHEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAPAAPAAPAKDGAKVHAGPAVRQLAREFGVELNAVSATGPHGRVLKEDVQAYVKTMMQK
CCCCCCCCCCCCCCEEECCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
AKEAPAGGASGGSGIPPIPEVDFSRFGEIEEVPMTRLMQLGASGLHRSWLNIPHVTQFDQ
HHCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCC
ADITDLEAFRVAQKGAAEKAGVKLTVLPLLLKSCAHLLKELPDFNASLAPSGKAVIRKKY
CCCHHHHHHHHHHHCCHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEE
VHIGFAVDTPDGLLVPVIRDVDQKSLLQLAAEAAALAEKARNKKLTANDMQGACFTISSL
EEEEEEEECCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHH
GHIGGTGFTPIVNAPEVAILGVSKATIQPVWDGKAFQPKLMLPLSLSYDHRVINGAAAAR
HCCCCCCCCCCCCCCCEEEEECCHHEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHH
FTKRLSELLADIRTILL
HHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9171401; 10984043 [H]