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The map label for this gene is alyA [H]

Identifier: 66043775

GI number: 66043775

Start: 563658

End: 564329

Strand: Reverse

Name: alyA [H]

Synonym: Psyr_0508

Alternate gene names: 66043775

Gene position: 564329-563658 (Counterclockwise)

Preceding gene: 66043779

Following gene: 161486739

Centisome position: 9.26

GC content: 52.23

Gene sequence:

>672_bases
ATGGTAGATCTTGGATCGTGGAATCTCAGCATCCCGGTGGGGTCGCCCCCCAAGACCATCGAAACCGCTCAGTTAGTAAA
AGGCTACGACGGTAAATATTTCAAATCCAAAACCGACGCCATTGTGTTCTGGTCGCCCGTCACCGGCACTAAAACCGAGA
ACGCCAAATATTCCCGAAGCGAACTTCGGGAAACGTGGTCCGATGGTTCGCTGCATAATTGGGTGTACCCGGATGCTGAC
AACTTTCTGAATGCCAAACTGGAAGTCGACCAAGTACCTTCCTCAGGCAAGATTGTCATCGGACAAATACACGCATACGA
GAGCACAGAGCCGCTCCTGAAAGTCGAGTTTCAATACAAGGAAGACACCAAGACCGGCGATATCGTTGCAAAAGTCCGCA
ACCGCTATGACGACGATGAAAGCAAGGTTTACACCATCGTAGAGGGTGTGAAGCTCAATGAGACATTCAATTACGTCATT
CACCTGAGCAAGGGCGGAAAACTGTCCGTCAGCGCCAAGGATCAGGACTGGAGCACGCGCATCAGTGAGACCTGGCGCGA
CAAGCCTCTGTACTTCAAGGCGGGGGTGTATACCCAGGACAATACCGGGTACACCTCGGAGGGTGGCAAGGCGACGTTCT
ACAACCTCGACATCGATCACAACGAGAGCTGA

Upstream 100 bases:

>100_bases
ATTAACAAATTGTCCTACAAGCCGTATAAAAGCTGGCTGAAGCCTCGTGCCAGAGGCTTTCTCTATGAGCGATACAAAGA
CATTTACGGACGGAGACAAA

Downstream 100 bases:

>100_bases
GCGGCACGCAGCTCTCAGCCTGAATACACAACGGCCCGCGCTTCACGAAGAAGAACGGGCCGTTTGTGTTTCAGTCAGAC
CTGTCAGCGGTTGGCTTTGA

Product: lyase, putative

Products: NA

Alternate protein names: Poly(beta-D-mannuronate) lyase; Poly(mana) alginate lyase [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MVDLGSWNLSIPVGSPPKTIETAQLVKGYDGKYFKSKTDAIVFWSPVTGTKTENAKYSRSELRETWSDGSLHNWVYPDAD
NFLNAKLEVDQVPSSGKIVIGQIHAYESTEPLLKVEFQYKEDTKTGDIVAKVRNRYDDDESKVYTIVEGVKLNETFNYVI
HLSKGGKLSVSAKDQDWSTRISETWRDKPLYFKAGVYTQDNTGYTSEGGKATFYNLDIDHNES

Sequences:

>Translated_223_residues
MVDLGSWNLSIPVGSPPKTIETAQLVKGYDGKYFKSKTDAIVFWSPVTGTKTENAKYSRSELRETWSDGSLHNWVYPDAD
NFLNAKLEVDQVPSSGKIVIGQIHAYESTEPLLKVEFQYKEDTKTGDIVAKVRNRYDDDESKVYTIVEGVKLNETFNYVI
HLSKGGKLSVSAKDQDWSTRISETWRDKPLYFKAGVYTQDNTGYTSEGGKATFYNLDIDHNES
>Mature_223_residues
MVDLGSWNLSIPVGSPPKTIETAQLVKGYDGKYFKSKTDAIVFWSPVTGTKTENAKYSRSELRETWSDGSLHNWVYPDAD
NFLNAKLEVDQVPSSGKIVIGQIHAYESTEPLLKVEFQYKEDTKTGDIVAKVRNRYDDDESKVYTIVEGVKLNETFNYVI
HLSKGGKLSVSAKDQDWSTRISETWRDKPLYFKAGVYTQDNTGYTSEGGKATFYNLDIDHNES

Specific function: Degrades alginates that contain guluronic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide lyase 7 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014895
- InterPro:   IPR008985
- InterPro:   IPR013320 [H]

Pfam domain/function: PF08787 Alginate_lyase2 [H]

EC number: =4.2.2.3 [H]

Molecular weight: Translated: 25203; Mature: 25203

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDLGSWNLSIPVGSPPKTIETAQLVKGYDGKYFKSKTDAIVFWSPVTGTKTENAKYSRS
CCCCCCEEEEEECCCCCCCCHHHHHHCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCHH
ELRETWSDGSLHNWVYPDADNFLNAKLEVDQVPSSGKIVIGQIHAYESTEPLLKVEFQYK
HHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEEEEC
EDTKTGDIVAKVRNRYDDDESKVYTIVEGVKLNETFNYVIHLSKGGKLSVSAKDQDWSTR
CCCCCHHHHHHHHHCCCCCCCEEEEEEECEEECCEEEEEEEECCCCEEEEEECCCCCHHH
ISETWRDKPLYFKAGVYTQDNTGYTSEGGKATFYNLDIDHNES
HHHHHCCCCEEEEECEEEECCCCEECCCCEEEEEEEEECCCCC
>Mature Secondary Structure
MVDLGSWNLSIPVGSPPKTIETAQLVKGYDGKYFKSKTDAIVFWSPVTGTKTENAKYSRS
CCCCCCEEEEEECCCCCCCCHHHHHHCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCHH
ELRETWSDGSLHNWVYPDADNFLNAKLEVDQVPSSGKIVIGQIHAYESTEPLLKVEFQYK
HHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEEEEC
EDTKTGDIVAKVRNRYDDDESKVYTIVEGVKLNETFNYVIHLSKGGKLSVSAKDQDWSTR
CCCCCHHHHHHHHHCCCCCCCEEEEEEECEEECCEEEEEEEECCCCEEEEEECCCCCHHH
ISETWRDKPLYFKAGVYTQDNTGYTSEGGKATFYNLDIDHNES
HHHHHCCCCEEEEECEEEECCCCEECCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8200539 [H]