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The map label for this gene is metF [H]

Identifier: 66043726

GI number: 66043726

Start: 497145

End: 497990

Strand: Reverse

Name: metF [H]

Synonym: Psyr_0459

Alternate gene names: 66043726

Gene position: 497990-497145 (Counterclockwise)

Preceding gene: 66043727

Following gene: 66043725

Centisome position: 8.17

GC content: 59.81

Gene sequence:

>846_bases
ATGTCTCAAGAACGCCGCTTCAGCTTCGAGTTCTTTCCTACCAAGACCGACGCTGGACACGAAAAGCTGCTGACCGTCGC
TCGTCAGTTGGCCACGTACAACCCCGACTTTTTCTCCTGCACCTATGGTGCCGGTGGCTCGACCCGCGACCGCACGCTCA
ATACCGTGCTGCAGCTGGAAAACGAGATCAAAGTCCCTGCTGCGCCGCATTTGTCCTGCGTAGGCGACAGCAAGGATGAC
CTGCGCAACCTGTTGGCGCAGTACAAGGATGCGGGCATCAAACGCATCGTGGCACTGCGCGGCGACCTGCCATCCGGCAT
GGGCATGGCCAGCGGTGAACTGCGTCACGCCAATGACCTGGTGAGCTTCATTCGCCAGGAAAGCGGCAGCCATTTCCACA
TCGAAGTCGCCGCCTACCCGGAAATGCATCCGCAGGCGCGCAATTTCGAAGACGACCTCAAGCACTTCGTCAACAAGGCC
AACGCCGGCGCCGACAGTGCGATCACGCAGTACTTCTTCAACGCCGACAGCTATTTCAACTTCGTCGAGCGGGTCGAGAA
GATGGGCGTCAGCATTCCGATCGTGCCGGGCATCATGCCGATCACCAACTACAGCAAGCTGGCGCGTTTCTCGGATGCCT
GCGGTGCGGAAATCCCGCGCTGGATCCGTAAACAGCTGGAAGCCTACGGCGATGACGTGCAAAGCATCCAGGCGTTTGGC
GAAGAAGTCATCACGCAGATGTGCGAACGACTGCTGCAAGGCGGCGCCCCCGGCCTGCATTTCTACACGCTGAACCAGGC
AGAGCCGAGCCTGGCGGTGTGGAACAACCTGCAACTGCCACGCTGA

Upstream 100 bases:

>100_bases
ATTACACAGCCGTGAGCGGTAAGCATGAGGCTGCGGAGCCAGGCATACAGCCCTCTTGCAGCTTGTAGCTTGCAGCTAAA
AGCTGGAGGCCTCCTCCCCC

Downstream 100 bases:

>100_bases
AAACGGCTGCGGCACCGCGATCCGACCGGCAGTTTCCGGGGGATCGCGGCCTCATACACCGTTGGGCAGCAGGAAAGCGG
CGTATTGCGCTCGCTCTGTT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSQERRFSFEFFPTKTDAGHEKLLTVARQLATYNPDFFSCTYGAGGSTRDRTLNTVLQLENEIKVPAAPHLSCVGDSKDD
LRNLLAQYKDAGIKRIVALRGDLPSGMGMASGELRHANDLVSFIRQESGSHFHIEVAAYPEMHPQARNFEDDLKHFVNKA
NAGADSAITQYFFNADSYFNFVERVEKMGVSIPIVPGIMPITNYSKLARFSDACGAEIPRWIRKQLEAYGDDVQSIQAFG
EEVITQMCERLLQGGAPGLHFYTLNQAEPSLAVWNNLQLPR

Sequences:

>Translated_281_residues
MSQERRFSFEFFPTKTDAGHEKLLTVARQLATYNPDFFSCTYGAGGSTRDRTLNTVLQLENEIKVPAAPHLSCVGDSKDD
LRNLLAQYKDAGIKRIVALRGDLPSGMGMASGELRHANDLVSFIRQESGSHFHIEVAAYPEMHPQARNFEDDLKHFVNKA
NAGADSAITQYFFNADSYFNFVERVEKMGVSIPIVPGIMPITNYSKLARFSDACGAEIPRWIRKQLEAYGDDVQSIQAFG
EEVITQMCERLLQGGAPGLHFYTLNQAEPSLAVWNNLQLPR
>Mature_280_residues
SQERRFSFEFFPTKTDAGHEKLLTVARQLATYNPDFFSCTYGAGGSTRDRTLNTVLQLENEIKVPAAPHLSCVGDSKDDL
RNLLAQYKDAGIKRIVALRGDLPSGMGMASGELRHANDLVSFIRQESGSHFHIEVAAYPEMHPQARNFEDDLKHFVNKAN
AGADSAITQYFFNADSYFNFVERVEKMGVSIPIVPGIMPITNYSKLARFSDACGAEIPRWIRKQLEAYGDDVQSIQAFGE
EVITQMCERLLQGGAPGLHFYTLNQAEPSLAVWNNLQLPR

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=272, Percent_Identity=37.5, Blast_Score=178, Evalue=6e-45,
Organism=Escherichia coli, GI1790377, Length=270, Percent_Identity=38.1481481481481, Blast_Score=213, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI25147436, Length=270, Percent_Identity=34.8148148148148, Blast_Score=168, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25147441, Length=270, Percent_Identity=34.8148148148148, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6325234, Length=276, Percent_Identity=33.695652173913, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321313, Length=280, Percent_Identity=30.7142857142857, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24662658, Length=229, Percent_Identity=24.4541484716157, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31399; Mature: 31268

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQERRFSFEFFPTKTDAGHEKLLTVARQLATYNPDFFSCTYGAGGSTRDRTLNTVLQLE
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHH
NEIKVPAAPHLSCVGDSKDDLRNLLAQYKDAGIKRIVALRGDLPSGMGMASGELRHANDL
CCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
VSFIRQESGSHFHIEVAAYPEMHPQARNFEDDLKHFVNKANAGADSAITQYFFNADSYFN
HHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHH
FVERVEKMGVSIPIVPGIMPITNYSKLARFSDACGAEIPRWIRKQLEAYGDDVQSIQAFG
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHH
EEVITQMCERLLQGGAPGLHFYTLNQAEPSLAVWNNLQLPR
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
SQERRFSFEFFPTKTDAGHEKLLTVARQLATYNPDFFSCTYGAGGSTRDRTLNTVLQLE
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHH
NEIKVPAAPHLSCVGDSKDDLRNLLAQYKDAGIKRIVALRGDLPSGMGMASGELRHANDL
CCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
VSFIRQESGSHFHIEVAAYPEMHPQARNFEDDLKHFVNKANAGADSAITQYFFNADSYFN
HHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHH
FVERVEKMGVSIPIVPGIMPITNYSKLARFSDACGAEIPRWIRKQLEAYGDDVQSIQAFG
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHH
EEVITQMCERLLQGGAPGLHFYTLNQAEPSLAVWNNLQLPR
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]