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The map label for this gene is ynfL [H]

Identifier: 66043688

GI number: 66043688

Start: 460420

End: 461310

Strand: Direct

Name: ynfL [H]

Synonym: Psyr_0421

Alternate gene names: 66043688

Gene position: 460420-461310 (Clockwise)

Preceding gene: 66043681

Following gene: 66043707

Centisome position: 7.56

GC content: 62.96

Gene sequence:

>891_bases
ATGGAATTGCGTCATCTGCGTTACTTCATCGCGGTTGCCGAGGAGCTGCATTTTGGCCGCGCAGCCCAGGCGCTGGGGAT
TTCGCAACCGCCGCTCAGCCAGCAGATTCAGACGCTGGAGCAGGAACTGGGCACGCGTCTTTTCGAACGCACCAACCGGC
GTGTCGAGCTGAGCGAATCCGGGCGGCTGTTTCTCGACGAAGCACGGCTGGTGCTGGCACAGGTCGACAAGGCCACTGAT
GTTGCCCGGCGTGCGCAACTGGGTGAAATCGGCGAGCTGAAGATTGGCTTCACTTCGTCTGCGCCGTTTACGTCGAGCAT
CCCGCAGGCGATTTTTGCTTTCCGTCAGGCCTGCCCGGATGTGCACCTGACCCTGACCGAGATCACCAGTCAGGAAGTGG
CGGCAGGTCTTGAGGACAAGACCATTCAGGTCGGCATCATGCGCCCGCTGGCATTGCCCGATTCACTGGTGGTGTTCGAA
TTGCTGAGCGAGCCGCTGGTGGCGATCATGCGTGCCGACCATCCGCTGGCGGCAGAAAGCGAGGAGGGGATCTACATGGC
GGCACTGGCCGCCGAGCCGTTCGTGTTCTTCCCGCGCACTTATGGCAGCGGTATCTACGCGCAAGTCCTGAGCCTGGCGC
GCGCCGCAGGGTTCAGCCCGCTGATCACCCAGGAAGCGGGCGAAGTGATGACCATCATCGGCCTGGTCGCAGCAGGCTTG
GGCGTCACCGTACTGCCCGCGTCCTACCGGCGAATGCGCATCGACGGCGTGGTCTACCGCAATGTCCTCGACCCCGGCGC
AACCAGCGCGGTGTGGCTGGTGCAACGCAAGGACGAACAATCGCCCATGGCCAGGGCGTTCACCGAGCTGTTAACGCGCA
ACGTAGGGTGA

Upstream 100 bases:

>100_bases
CGGTTGAGCAGGCAATGAAAAAATCATATAGCTCGCTAATCATTCCATCCAATATATTGTTCGACCTGTTTGATAGGTAA
AACGACTTAATGGAGGCCGC

Downstream 100 bases:

>100_bases
TTCAAAGGCACGGATGATAGGACGCGGAGCGTCCAGAACGGCATGCGACGCGGAGCGTCGCACGATAGTTGAGATTCTGG
CCGCAGGCCGTGGGAGCGAG

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MELRHLRYFIAVAEELHFGRAAQALGISQPPLSQQIQTLEQELGTRLFERTNRRVELSESGRLFLDEARLVLAQVDKATD
VARRAQLGEIGELKIGFTSSAPFTSSIPQAIFAFRQACPDVHLTLTEITSQEVAAGLEDKTIQVGIMRPLALPDSLVVFE
LLSEPLVAIMRADHPLAAESEEGIYMAALAAEPFVFFPRTYGSGIYAQVLSLARAAGFSPLITQEAGEVMTIIGLVAAGL
GVTVLPASYRRMRIDGVVYRNVLDPGATSAVWLVQRKDEQSPMARAFTELLTRNVG

Sequences:

>Translated_296_residues
MELRHLRYFIAVAEELHFGRAAQALGISQPPLSQQIQTLEQELGTRLFERTNRRVELSESGRLFLDEARLVLAQVDKATD
VARRAQLGEIGELKIGFTSSAPFTSSIPQAIFAFRQACPDVHLTLTEITSQEVAAGLEDKTIQVGIMRPLALPDSLVVFE
LLSEPLVAIMRADHPLAAESEEGIYMAALAAEPFVFFPRTYGSGIYAQVLSLARAAGFSPLITQEAGEVMTIIGLVAAGL
GVTVLPASYRRMRIDGVVYRNVLDPGATSAVWLVQRKDEQSPMARAFTELLTRNVG
>Mature_296_residues
MELRHLRYFIAVAEELHFGRAAQALGISQPPLSQQIQTLEQELGTRLFERTNRRVELSESGRLFLDEARLVLAQVDKATD
VARRAQLGEIGELKIGFTSSAPFTSSIPQAIFAFRQACPDVHLTLTEITSQEVAAGLEDKTIQVGIMRPLALPDSLVVFE
LLSEPLVAIMRADHPLAAESEEGIYMAALAAEPFVFFPRTYGSGIYAQVLSLARAAGFSPLITQEAGEVMTIIGLVAAGL
GVTVLPASYRRMRIDGVVYRNVLDPGATSAVWLVQRKDEQSPMARAFTELLTRNVG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=288, Percent_Identity=51.3888888888889, Blast_Score=284, Evalue=6e-78,
Organism=Escherichia coli, GI1788887, Length=287, Percent_Identity=34.8432055749129, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI2367136, Length=262, Percent_Identity=34.3511450381679, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1790399, Length=296, Percent_Identity=33.1081081081081, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI157672245, Length=297, Percent_Identity=29.96632996633, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI145693105, Length=292, Percent_Identity=26.027397260274, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=27.9352226720648, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790208, Length=291, Percent_Identity=30.2405498281787, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87081904, Length=201, Percent_Identity=31.8407960199005, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787530, Length=248, Percent_Identity=28.2258064516129, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788297, Length=300, Percent_Identity=27.3333333333333, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787601, Length=308, Percent_Identity=28.2467532467532, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082132, Length=251, Percent_Identity=27.0916334661355, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790262, Length=294, Percent_Identity=26.530612244898, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788481, Length=246, Percent_Identity=27.2357723577236, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788296, Length=242, Percent_Identity=30.1652892561983, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787589, Length=187, Percent_Identity=32.0855614973262, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1789173, Length=132, Percent_Identity=32.5757575757576, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32353; Mature: 32353

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFIAVAEELHFGRAAQALGISQPPLSQQIQTLEQELGTRLFERTNRRVELSES
CCHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCC
GRLFLDEARLVLAQVDKATDVARRAQLGEIGELKIGFTSSAPFTSSIPQAIFAFRQACPD
CCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
VHLTLTEITSQEVAAGLEDKTIQVGIMRPLALPDSLVVFELLSEPLVAIMRADHPLAAES
CEEEHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
EEGIYMAALAAEPFVFFPRTYGSGIYAQVLSLARAAGFSPLITQEAGEVMTIIGLVAAGL
CCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
GVTVLPASYRRMRIDGVVYRNVLDPGATSAVWLVQRKDEQSPMARAFTELLTRNVG
CEEEECCCHHHEEHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MELRHLRYFIAVAEELHFGRAAQALGISQPPLSQQIQTLEQELGTRLFERTNRRVELSES
CCHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCC
GRLFLDEARLVLAQVDKATDVARRAQLGEIGELKIGFTSSAPFTSSIPQAIFAFRQACPD
CCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
VHLTLTEITSQEVAAGLEDKTIQVGIMRPLALPDSLVVFELLSEPLVAIMRADHPLAAES
CEEEHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
EEGIYMAALAAEPFVFFPRTYGSGIYAQVLSLARAAGFSPLITQEAGEVMTIIGLVAAGL
CCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
GVTVLPASYRRMRIDGVVYRNVLDPGATSAVWLVQRKDEQSPMARAFTELLTRNVG
CEEEECCCHHHEEHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]