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The map label for this gene is maeB [H]

Identifier: 66043668

GI number: 66043668

Start: 429120

End: 430388

Strand: Direct

Name: maeB [H]

Synonym: Psyr_0401

Alternate gene names: 66043668

Gene position: 429120-430388 (Clockwise)

Preceding gene: 66043667

Following gene: 66043670

Centisome position: 7.04

GC content: 59.1

Gene sequence:

>1269_bases
ATGTCAGATCTGAAAACTGCCGCGCTCGAATACCATGCCAATCCTCGTCCGGGGAAGCTGAGCGTCGAACTCACCAAGCC
GACCGCAACCGCTCGTGACCTGGCTCTGGCCTACAGCCCTGGCGTTGCTGAACCCGTGCGTGAAATTGCCCGTGACCCCG
AGCTGGCTTACAAGTACACCGGCAAAGGCAATCTGGTAGCAGTTATTTCTGATGGCACCGCGATTCTCGGCCTCGGCGAT
CTGGGGCCATTGGCTTCCAAGCCGGTCATGGAAGGTAAGGGCGTTCTGTTCAAGCGCTTCGCCGGTATCGACGTTTTCGA
TATCGAAGTCGATTCCGAAAGCCCGCAGGCGTTCATCGACACCGTCAAGCGTATCTCCATCACCTTCGGTGGCATCAACC
TGGAAGACATCAAGGCGCCTGAGTGCTTTGAAATCGAGAAGGCGCTGATCGAACAGTGCGACATCCCGGTTTTCCATGAT
GACCAGCACGGCACCGCTATCGTGACGGCGGCCGGCATGATCAACGCACTGGAAATCGTCGGCAAGACCCTCGAATCGGC
GAAGATCGTCTGCCTGGGCGCTGGCGCTGCAGCCATCTCCTGCATGAAGCTGCTGGTGAGCATGGGCGCGAAGATCGAAA
ACATCTTCATGGTCGACCGTACCGGCGTTATCCACTCCGGCCGTACCGACCTGAACCAGTACAAGGCTGCATTCGCTCAC
GCTACCGAGAAGCGCTCGCTGACCGATGCACTGGATGGCGCTGACGTATTCGTAGGCCTGTCCGGCCCTAACCTGCTGAG
CGCTGAAAACCTCAAGCTGATGGCCAAGGACCCGATCGTGTTCGCTTGCTCGAACCCTGATCCGGAAATCTCCCCGGAAC
TGGCTCACGCCACGCGCAGCGATGTGATCATGGCCACTGGCCGTTCGGACTACCCGAACCAGGTCAACAACGTGCTGGGC
TTCCCGTTCATCTTCCGCGGTGCTCTGGACGTTCGCGCCAAGCGCATCAACGAAGAAATGAAAATCGCTGCGGCCAACGC
CCTGCGCGAACTGGCCAAGCTGCCTGTGCCACAGGAAGTCAGCGACGCCTACGGCGGCATCAAACTGGAATTCGGTCGTG
AGTACATCATCCCGAAACCAATGGATACGCGCCTGCTGGGCCTGATCGCCGATGCAGTGGCCAAGGCCGCTATCGAAAGC
GGCGTTGCCACCCTGCCTTATCCGAAGCACTACCCGCTGACCAGCGTGGATGACGTGTTCAAGGGCTGA

Upstream 100 bases:

>100_bases
CCTATTGACAGCAGTGACTGGCCAGTCTTGTTAGGACTATGCATCTTGCGTATCCTCGGCGGTCCGTCTGTCCCACAGTA
AAAAAAGCGGAATCCAATAC

Downstream 100 bases:

>100_bases
TCCCTGAGCCAATAAAAAACCCCTGCAGCGATGCAGGGGTTTTTTTATGCCTTGGATACCCTGCACTTGTGGGGCATTTT
CGGGATTGAACTGGCCTCTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MSDLKTAALEYHANPRPGKLSVELTKPTATARDLALAYSPGVAEPVREIARDPELAYKYTGKGNLVAVISDGTAILGLGD
LGPLASKPVMEGKGVLFKRFAGIDVFDIEVDSESPQAFIDTVKRISITFGGINLEDIKAPECFEIEKALIEQCDIPVFHD
DQHGTAIVTAAGMINALEIVGKTLESAKIVCLGAGAAAISCMKLLVSMGAKIENIFMVDRTGVIHSGRTDLNQYKAAFAH
ATEKRSLTDALDGADVFVGLSGPNLLSAENLKLMAKDPIVFACSNPDPEISPELAHATRSDVIMATGRSDYPNQVNNVLG
FPFIFRGALDVRAKRINEEMKIAAANALRELAKLPVPQEVSDAYGGIKLEFGREYIIPKPMDTRLLGLIADAVAKAAIES
GVATLPYPKHYPLTSVDDVFKG

Sequences:

>Translated_422_residues
MSDLKTAALEYHANPRPGKLSVELTKPTATARDLALAYSPGVAEPVREIARDPELAYKYTGKGNLVAVISDGTAILGLGD
LGPLASKPVMEGKGVLFKRFAGIDVFDIEVDSESPQAFIDTVKRISITFGGINLEDIKAPECFEIEKALIEQCDIPVFHD
DQHGTAIVTAAGMINALEIVGKTLESAKIVCLGAGAAAISCMKLLVSMGAKIENIFMVDRTGVIHSGRTDLNQYKAAFAH
ATEKRSLTDALDGADVFVGLSGPNLLSAENLKLMAKDPIVFACSNPDPEISPELAHATRSDVIMATGRSDYPNQVNNVLG
FPFIFRGALDVRAKRINEEMKIAAANALRELAKLPVPQEVSDAYGGIKLEFGREYIIPKPMDTRLLGLIADAVAKAAIES
GVATLPYPKHYPLTSVDDVFKG
>Mature_421_residues
SDLKTAALEYHANPRPGKLSVELTKPTATARDLALAYSPGVAEPVREIARDPELAYKYTGKGNLVAVISDGTAILGLGDL
GPLASKPVMEGKGVLFKRFAGIDVFDIEVDSESPQAFIDTVKRISITFGGINLEDIKAPECFEIEKALIEQCDIPVFHDD
QHGTAIVTAAGMINALEIVGKTLESAKIVCLGAGAAAISCMKLLVSMGAKIENIFMVDRTGVIHSGRTDLNQYKAAFAHA
TEKRSLTDALDGADVFVGLSGPNLLSAENLKLMAKDPIVFACSNPDPEISPELAHATRSDVIMATGRSDYPNQVNNVLGF
PFIFRGALDVRAKRINEEMKIAAANALRELAKLPVPQEVSDAYGGIKLEFGREYIIPKPMDTRLLGLIADAVAKAAIESG
VATLPYPKHYPLTSVDDVFKG

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=404, Percent_Identity=28.7128712871287, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI62420882, Length=371, Percent_Identity=26.9541778975741, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI62420880, Length=371, Percent_Identity=26.9541778975741, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI239049447, Length=371, Percent_Identity=26.9541778975741, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4505145, Length=347, Percent_Identity=27.3775216138329, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI270265879, Length=321, Percent_Identity=28.0373831775701, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788806, Length=406, Percent_Identity=60.8374384236453, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI87081919, Length=288, Percent_Identity=28.8194444444444, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17537199, Length=409, Percent_Identity=28.1173594132029, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78707242, Length=394, Percent_Identity=26.3959390862944, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI78707238, Length=394, Percent_Identity=26.3959390862944, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24646388, Length=346, Percent_Identity=30.3468208092486, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24646386, Length=346, Percent_Identity=30.3468208092486, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78707240, Length=392, Percent_Identity=26.2755102040816, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281362672, Length=457, Percent_Identity=27.3522975929978, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356279, Length=457, Percent_Identity=27.3522975929978, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281362674, Length=457, Percent_Identity=27.3522975929978, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 45120; Mature: 44989

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLKTAALEYHANPRPGKLSVELTKPTATARDLALAYSPGVAEPVREIARDPELAYKYT
CCCHHCCHHEECCCCCCCEEEEEEECCCCCHHHEEEEECCCCHHHHHHHHCCCCEEEEEC
GKGNLVAVISDGTAILGLGDLGPLASKPVMEGKGVLFKRFAGIDVFDIEVDSESPQAFID
CCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCEEHHHCCCEEEEEEECCCCCHHHHH
TVKRISITFGGINLEDIKAPECFEIEKALIEQCDIPVFHDDQHGTAIVTAAGMINALEIV
HHHEEEEEECCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHH
GKTLESAKIVCLGAGAAAISCMKLLVSMGAKIENIFMVDRTGVIHSGRTDLNQYKAAFAH
HHHHCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCEEECCCCCHHHHHHHHHH
ATEKRSLTDALDGADVFVGLSGPNLLSAENLKLMAKDPIVFACSNPDPEISPELAHATRS
HHHHHHHHHHCCCCEEEEECCCCCEECCCCCEEEECCCEEEEECCCCCCCCCHHHHCCCC
DVIMATGRSDYPNQVNNVLGFPFIFRGALDVRAKRINEEMKIAAANALRELAKLPVPQEV
CEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHH
SDAYGGIKLEFGREYIIPKPMDTRLLGLIADAVAKAAIESGVATLPYPKHYPLTSVDDVF
HHHHCCEEEEECCEECCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHH
KG
CC
>Mature Secondary Structure 
SDLKTAALEYHANPRPGKLSVELTKPTATARDLALAYSPGVAEPVREIARDPELAYKYT
CCHHCCHHEECCCCCCCEEEEEEECCCCCHHHEEEEECCCCHHHHHHHHCCCCEEEEEC
GKGNLVAVISDGTAILGLGDLGPLASKPVMEGKGVLFKRFAGIDVFDIEVDSESPQAFID
CCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCEEHHHCCCEEEEEEECCCCCHHHHH
TVKRISITFGGINLEDIKAPECFEIEKALIEQCDIPVFHDDQHGTAIVTAAGMINALEIV
HHHEEEEEECCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHH
GKTLESAKIVCLGAGAAAISCMKLLVSMGAKIENIFMVDRTGVIHSGRTDLNQYKAAFAH
HHHHCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCEEECCCCCHHHHHHHHHH
ATEKRSLTDALDGADVFVGLSGPNLLSAENLKLMAKDPIVFACSNPDPEISPELAHATRS
HHHHHHHHHHCCCCEEEEECCCCCEECCCCCEEEECCCEEEEECCCCCCCCCHHHHCCCC
DVIMATGRSDYPNQVNNVLGFPFIFRGALDVRAKRINEEMKIAAANALRELAKLPVPQEV
CEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHH
SDAYGGIKLEFGREYIIPKPMDTRLLGLIADAVAKAAIESGVATLPYPKHYPLTSVDDVF
HHHHCCEEEEECCEECCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHH
KG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]