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The map label for this gene is yokF [H]

Identifier: 66043667

GI number: 66043667

Start: 428135

End: 428938

Strand: Direct

Name: yokF [H]

Synonym: Psyr_0400

Alternate gene names: 66043667

Gene position: 428135-428938 (Clockwise)

Preceding gene: 66043666

Following gene: 66043668

Centisome position: 7.03

GC content: 64.93

Gene sequence:

>804_bases
ATGGGCATCTCCAGGCTGCTTGAAAAGGCGCTCCTTGTGGGCGTCTTTTTTATGCCCGCGATTTGGGTGTCCGCTGCGCA
GGCCTTCTGCCCCGCACCTGCCTCCTTGCCGATTGCACAGGTGCAGCGTGTGGTCGACGGCGACACGTTAAAGCTGACCG
ATGGCCGCAGTGTGCGGATGATCGGGCTCAATACGCCTGAAACCGGCAGGAAAGGCCGGTCGGCCGAACCGTTTGCCGAG
GCGGCGAAAAAGCGCTTGCAGACGCTGGTGAATGAAAGCGGCGGCAAGGTCAGGCTGCGTATCGGCCAGCAGGGCAAGGA
TCATTACGGTCGCACGCTGGCCAACGTCTATGACCGTAAGGGCGCCAACCTCGAGGCGCAGTTGCTGAGTGAAGGTCTGG
GCTATCTGGTGGCCGTTGCGCCCAATGTCAGGCTGGTCGCTTGCCAGCAGGGCGCGGAGCGTCAGGCACGTGAGGCGCGG
CTGGGTGTGTGGCGCAATTCGCCGGTGCAGTCCTCGACCCGGCTGAGCAAAGGCGGGTTCGCGATTGTGTCCGGTCAGGT
CAGGCGTGTGCAGCGCAATCGCGGCGGTATCTGGATCGAGTTGCAGGGCTCGCTGGTCCTGCGTGTCGCGCCTGCCCACC
TGAATGCGTTCGATACGGCGATGCTCCAGCGCCTCGAAGGGCAGCAGGTCGAAGCGCGTGGCTGGGTAGTCGACCGCTCC
CGGCGCGCGGGCCTGAAAGCCGGGCAGTCCCGCTGGCTGCTGCCACTGACCCATCCCGCAATGCTGAGCCCTTCCCGCCG
TTAG

Upstream 100 bases:

>100_bases
GGCAAGCAGAAGATGCTTGACGTTGGCGGCCGTGTCGACAAGTTCAAATCCCGTTTCGGTGCGTTCGGCGCAACCAAAGC
GAAGTAAGACTGGCTGCCCT

Downstream 100 bases:

>100_bases
AAGCGCCCTTCAGGCCGGTGATCCGACGAGCGTGACCGAGTGTTCACGTATCAGGCCTCTAAGCTAAAGTCTAAGCCTGT
GCCTATTGACAGCAGTGACT

Product: nuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MGISRLLEKALLVGVFFMPAIWVSAAQAFCPAPASLPIAQVQRVVDGDTLKLTDGRSVRMIGLNTPETGRKGRSAEPFAE
AAKKRLQTLVNESGGKVRLRIGQQGKDHYGRTLANVYDRKGANLEAQLLSEGLGYLVAVAPNVRLVACQQGAERQAREAR
LGVWRNSPVQSSTRLSKGGFAIVSGQVRRVQRNRGGIWIELQGSLVLRVAPAHLNAFDTAMLQRLEGQQVEARGWVVDRS
RRAGLKAGQSRWLLPLTHPAMLSPSRR

Sequences:

>Translated_267_residues
MGISRLLEKALLVGVFFMPAIWVSAAQAFCPAPASLPIAQVQRVVDGDTLKLTDGRSVRMIGLNTPETGRKGRSAEPFAE
AAKKRLQTLVNESGGKVRLRIGQQGKDHYGRTLANVYDRKGANLEAQLLSEGLGYLVAVAPNVRLVACQQGAERQAREAR
LGVWRNSPVQSSTRLSKGGFAIVSGQVRRVQRNRGGIWIELQGSLVLRVAPAHLNAFDTAMLQRLEGQQVEARGWVVDRS
RRAGLKAGQSRWLLPLTHPAMLSPSRR
>Mature_266_residues
GISRLLEKALLVGVFFMPAIWVSAAQAFCPAPASLPIAQVQRVVDGDTLKLTDGRSVRMIGLNTPETGRKGRSAEPFAEA
AKKRLQTLVNESGGKVRLRIGQQGKDHYGRTLANVYDRKGANLEAQLLSEGLGYLVAVAPNVRLVACQQGAERQAREARL
GVWRNSPVQSSTRLSKGGFAIVSGQVRRVQRNRGGIWIELQGSLVLRVAPAHLNAFDTAMLQRLEGQQVEARGWVVDRSR
RAGLKAGQSRWLLPLTHPAMLSPSRR

Specific function: Catalyzes the hydrolysis of supercoiled double and single strand DNA and RNA. Involved in chromosomal DNA degradation and cell death caused by thermal stress [H]

COG id: COG1525

COG function: function code L; Micrococcal nuclease (thermonuclease) homologs

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008613
- InterPro:   IPR016071
- InterPro:   IPR006021
- InterPro:   IPR002071 [H]

Pfam domain/function: PF05901 Excalibur; PF00565 SNase [H]

EC number: NA

Molecular weight: Translated: 29158; Mature: 29027

Theoretical pI: Translated: 11.88; Mature: 11.88

Prosite motif: PS50830 TNASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISRLLEKALLVGVFFMPAIWVSAAQAFCPAPASLPIAQVQRVVDGDTLKLTDGRSVRM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCEEEE
IGLNTPETGRKGRSAEPFAEAAKKRLQTLVNESGGKVRLRIGQQGKDHYGRTLANVYDRK
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
GANLEAQLLSEGLGYLVAVAPNVRLVACQQGAERQAREARLGVWRNSPVQSSTRLSKGGF
CCCHHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
AIVSGQVRRVQRNRGGIWIELQGSLVLRVAPAHLNAFDTAMLQRLEGQQVEARGWVVDRS
EEECHHHHHHHHCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHCCCEEECCCEEEECC
RRAGLKAGQSRWLLPLTHPAMLSPSRR
HHCCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
GISRLLEKALLVGVFFMPAIWVSAAQAFCPAPASLPIAQVQRVVDGDTLKLTDGRSVRM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCEEEE
IGLNTPETGRKGRSAEPFAEAAKKRLQTLVNESGGKVRLRIGQQGKDHYGRTLANVYDRK
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
GANLEAQLLSEGLGYLVAVAPNVRLVACQQGAERQAREARLGVWRNSPVQSSTRLSKGGF
CCCHHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
AIVSGQVRRVQRNRGGIWIELQGSLVLRVAPAHLNAFDTAMLQRLEGQQVEARGWVVDRS
EEECHHHHHHHHCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHCCCEEECCCEEEECC
RRAGLKAGQSRWLLPLTHPAMLSPSRR
HHCCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]