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The map label for this gene is ascD [H]

Identifier: 66043573

GI number: 66043573

Start: 323173

End: 324141

Strand: Direct

Name: ascD [H]

Synonym: Psyr_0303

Alternate gene names: 66043573

Gene position: 323173-324141 (Clockwise)

Preceding gene: 66043572

Following gene: 66043574

Centisome position: 5.3

GC content: 63.05

Gene sequence:

>969_bases
ATGCGCGTAACCTTGCAGCCATCAGGTGCGGTGCTGGAAACCCTGCCGGGCGAGCGGATACTCGATGCTGCACAGCGTCT
GGGCTACGAGTGCCCGCAGAGCTGTCGTAATGGCAACTGCCATGTCTGCTCAGCCCTGCTGGTCGAAGGCCGGGTAATGC
AGTCAGGGCAGACGCTTGATCACGGCGAAATTCATACCTGTGTGGCCGAGCCGCTGACAGAGTGCTTGGTGCTGTGGGAT
GGCGTGTTGGCCAAGGGTGAACTGCCGGTGCGCAGTTTTGCCTGCCAATTGAGCGAATGCGTCGAGGTGGGTGGCGATGT
CTGGCGGGTCGGCCTGCGCGCCCCGGCCGGCAAGCCGCCGCGTTATCATGCCGGGCAGTACCTGATGATCGAGCGCGAGA
ACGGCGAGAAGTCGGCGTTTTCCATCGCGTCGGCGCCACACAGCGGTCGCGACCTGGAGCTGCACGTGCTGGCTCGTGAA
GACAGTGCGCGCAGCCTGATCGAACAACTGCAACGCAACCGCATGGCGCGGGTCGAGCTGCCCTTTGGCGACACCCACCT
CGCCGAGCTGCCGGACGGGCCGCTGGTGCTGATCGCGGCCGGTACGGGCATGGCGCAAATGAACAGTTTGTTGGAACACT
GTCGCTCCACAGGCTTCAGTCATCCTGTGCACCTGTATTGGGGCATGCGGCGTCCCGAGGATTTCTACCAGGTCAGCCAT
TGGGATGAGTGGGCGAAACTGCCGAACCTGTTCCTGCACAAGGTCGTCAGCGACCTGTGCGGTTGGGAGGGGCGTTGCGG
TCTGCTGCACGAGGCGGTTTGCGAGGATATCAAGGACCTGTCGGCCGTTCACGTCTACGCCAGCGGCTCGCCTGCGATGA
TCTACGGAACCCTGGACGCCCTGGTTGACTCCGGAATGGATGCACATCAGATGCGCGCAGACGTATTCGCCTACGCACCG
CGTCCGTAA

Upstream 100 bases:

>100_bases
GGCCAGGTGAGACCAGCCGCGAGTGGGGCAGGGCGATCGTCAAGGACGAAGCCACCACACGCCGGGTGGACGAAATCTGG
ACTCAGTTGGGAATAGACTG

Downstream 100 bases:

>100_bases
TGCCGGACTGACGGCTGCAGGAGTGGAGTGTAAATTGCTTTAACTGATTACAACCGATGGCGATGCCTGTTCGTCGCCAG
GCAACTTTTAGTAGAGGCAG

Product: CDP-6-deoxy-delta-3,4-glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: CDP-6-deoxy-delta-3,4-glucoseen reductase; E3 [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRVTLQPSGAVLETLPGERILDAAQRLGYECPQSCRNGNCHVCSALLVEGRVMQSGQTLDHGEIHTCVAEPLTECLVLWD
GVLAKGELPVRSFACQLSECVEVGGDVWRVGLRAPAGKPPRYHAGQYLMIERENGEKSAFSIASAPHSGRDLELHVLARE
DSARSLIEQLQRNRMARVELPFGDTHLAELPDGPLVLIAAGTGMAQMNSLLEHCRSTGFSHPVHLYWGMRRPEDFYQVSH
WDEWAKLPNLFLHKVVSDLCGWEGRCGLLHEAVCEDIKDLSAVHVYASGSPAMIYGTLDALVDSGMDAHQMRADVFAYAP
RP

Sequences:

>Translated_322_residues
MRVTLQPSGAVLETLPGERILDAAQRLGYECPQSCRNGNCHVCSALLVEGRVMQSGQTLDHGEIHTCVAEPLTECLVLWD
GVLAKGELPVRSFACQLSECVEVGGDVWRVGLRAPAGKPPRYHAGQYLMIERENGEKSAFSIASAPHSGRDLELHVLARE
DSARSLIEQLQRNRMARVELPFGDTHLAELPDGPLVLIAAGTGMAQMNSLLEHCRSTGFSHPVHLYWGMRRPEDFYQVSH
WDEWAKLPNLFLHKVVSDLCGWEGRCGLLHEAVCEDIKDLSAVHVYASGSPAMIYGTLDALVDSGMDAHQMRADVFAYAP
RP
>Mature_322_residues
MRVTLQPSGAVLETLPGERILDAAQRLGYECPQSCRNGNCHVCSALLVEGRVMQSGQTLDHGEIHTCVAEPLTECLVLWD
GVLAKGELPVRSFACQLSECVEVGGDVWRVGLRAPAGKPPRYHAGQYLMIERENGEKSAFSIASAPHSGRDLELHVLARE
DSARSLIEQLQRNRMARVELPFGDTHLAELPDGPLVLIAAGTGMAQMNSLLEHCRSTGFSHPVHLYWGMRRPEDFYQVSH
WDEWAKLPNLFLHKVVSDLCGWEGRCGLLHEAVCEDIKDLSAVHVYASGSPAMIYGTLDALVDSGMDAHQMRADVFAYAP
RP

Specific function: Participates in the conversion of CDP-6-deoxy-D-glycero- L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=233, Percent_Identity=28.3261802575107, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 35461; Mature: 35461

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTLQPSGAVLETLPGERILDAAQRLGYECPQSCRNGNCHVCSALLVEGRVMQSGQTLD
CEEEECCCCCHHHHCCCHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHCHHHCCCCCCC
HGEIHTCVAEPLTECLVLWDGVLAKGELPVRSFACQLSECVEVGGDVWRVGLRAPAGKPP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCCCCCC
RYHAGQYLMIERENGEKSAFSIASAPHSGRDLELHVLAREDSARSLIEQLQRNRMARVEL
CCCCCCEEEEECCCCCCHHEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEC
PFGDTHLAELPDGPLVLIAAGTGMAQMNSLLEHCRSTGFSHPVHLYWGMRRPEDFYQVSH
CCCCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
WDEWAKLPNLFLHKVVSDLCGWEGRCGLLHEAVCEDIKDLSAVHVYASGSPAMIYGTLDA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEEHHH
LVDSGMDAHQMRADVFAYAPRP
HHHCCCCHHHHHHHHEEECCCC
>Mature Secondary Structure
MRVTLQPSGAVLETLPGERILDAAQRLGYECPQSCRNGNCHVCSALLVEGRVMQSGQTLD
CEEEECCCCCHHHHCCCHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHCHHHCCCCCCC
HGEIHTCVAEPLTECLVLWDGVLAKGELPVRSFACQLSECVEVGGDVWRVGLRAPAGKPP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCCCCCC
RYHAGQYLMIERENGEKSAFSIASAPHSGRDLELHVLAREDSARSLIEQLQRNRMARVEL
CCCCCCEEEEECCCCCCHHEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEC
PFGDTHLAELPDGPLVLIAAGTGMAQMNSLLEHCRSTGFSHPVHLYWGMRRPEDFYQVSH
CCCCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
WDEWAKLPNLFLHKVVSDLCGWEGRCGLLHEAVCEDIKDLSAVHVYASGSPAMIYGTLDA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEEHHH
LVDSGMDAHQMRADVFAYAPRP
HHHCCCCHHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931327; 11586360; 12142430 [H]