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The map label for this gene is dsbA [H]

Identifier: 66043538

GI number: 66043538

Start: 285875

End: 286519

Strand: Reverse

Name: dsbA [H]

Synonym: Psyr_0268

Alternate gene names: 66043538

Gene position: 286519-285875 (Counterclockwise)

Preceding gene: 66043540

Following gene: 66043537

Centisome position: 4.7

GC content: 58.14

Gene sequence:

>645_bases
ATGCGTAATCTGATCATCAGCGCCGCTCTGGTTGCCGCCAGTCTGTTCGGTATGTCCGCCCAGGCCGCCACGCCCATCGA
GGCCGGCAAGCAATACGTCGAGCTGGCAAGCGCTGTGCCTGTTGCCGAGCCAGGCAAGATCGAAGTCGTCGAGCTGTTCT
GGTATGGCTGCCCGCATTGCTACGCATTCGAGCCAACCATCAATCCGTGGGTTGAAAAACTGCCGTCCGACGTGAAGTTC
GTACGCATCCCTGCCATGTTCGGCGGCCCTTGGGACGCTCACGGCCAGCTGTTCATTACCCTGGACACCATGGGCGTCGA
ACACAAGGTTCACGCAGCCGTATTCGAAGCCATCCAGAAAGGCGGCAAGCGTCTGACCGACAAGAATGACATGGCTGATT
TCGTCGCCACACAGGGCGTGAACAAGGAAGACTTCCTGAAGACGTTCGACTCTTTCGCAGTCAAGGGCAAGATCGCCCAG
TACAAGGAACTGGCCAAGAAGTACGAAGTGACCGGCGTTCCGACCATGATCGTCAATGGCAAGTACCGCTTTGACCTGGG
TTCTGCCGGTGGTCCGGAAAAGACCCTGGAAGTTGCCGACCAGTTGATTGCCAAAGAGCGAGCGGCTGCCGCAGCCGCCA
AGTAA

Upstream 100 bases:

>100_bases
CCATTTTTACAACCTCAGCGTTACACTAACGAACTCAGGCTTTCGCGTCCGGTCGAAACACCGTCGCTTGAAGGCGATTC
AATCTGCTCAGGAGTAAAGC

Downstream 100 bases:

>100_bases
GCGCACATCATGCGCCGCTGGGGCAAAGGCAGTACCCGTATCGTTGGCCTGCATGATCCGCAGGTCAACGAACATCATCT
GCTGCAAAACGGCCTGCCGC

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRNLIISAALVAASLFGMSAQAATPIEAGKQYVELASAVPVAEPGKIEVVELFWYGCPHCYAFEPTINPWVEKLPSDVKF
VRIPAMFGGPWDAHGQLFITLDTMGVEHKVHAAVFEAIQKGGKRLTDKNDMADFVATQGVNKEDFLKTFDSFAVKGKIAQ
YKELAKKYEVTGVPTMIVNGKYRFDLGSAGGPEKTLEVADQLIAKERAAAAAAK

Sequences:

>Translated_214_residues
MRNLIISAALVAASLFGMSAQAATPIEAGKQYVELASAVPVAEPGKIEVVELFWYGCPHCYAFEPTINPWVEKLPSDVKF
VRIPAMFGGPWDAHGQLFITLDTMGVEHKVHAAVFEAIQKGGKRLTDKNDMADFVATQGVNKEDFLKTFDSFAVKGKIAQ
YKELAKKYEVTGVPTMIVNGKYRFDLGSAGGPEKTLEVADQLIAKERAAAAAAK
>Mature_214_residues
MRNLIISAALVAASLFGMSAQAATPIEAGKQYVELASAVPVAEPGKIEVVELFWYGCPHCYAFEPTINPWVEKLPSDVKF
VRIPAMFGGPWDAHGQLFITLDTMGVEHKVHAAVFEAIQKGGKRLTDKNDMADFVATQGVNKEDFLKTFDSFAVKGKIAQ
YKELAKKYEVTGVPTMIVNGKYRFDLGSAGGPEKTLEVADQLIAKERAAAAAAK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=180, Percent_Identity=35, Blast_Score=87, Evalue=6e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23210; Mature: 23210

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLIISAALVAASLFGMSAQAATPIEAGKQYVELASAVPVAEPGKIEVVELFWYGCPHC
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCC
YAFEPTINPWVEKLPSDVKFVRIPAMFGGPWDAHGQLFITLDTMGVEHKVHAAVFEAIQK
EEECCCCCHHHHHCCCCCEEEEECCEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
GGKRLTDKNDMADFVATQGVNKEDFLKTFDSFAVKGKIAQYKELAKKYEVTGVPTMIVNG
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEECC
KYRFDLGSAGGPEKTLEVADQLIAKERAAAAAAK
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRNLIISAALVAASLFGMSAQAATPIEAGKQYVELASAVPVAEPGKIEVVELFWYGCPHC
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCC
YAFEPTINPWVEKLPSDVKFVRIPAMFGGPWDAHGQLFITLDTMGVEHKVHAAVFEAIQK
EEECCCCCHHHHHCCCCCEEEEECCEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
GGKRLTDKNDMADFVATQGVNKEDFLKTFDSFAVKGKIAQYKELAKKYEVTGVPTMIVNG
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEECC
KYRFDLGSAGGPEKTLEVADQLIAKERAAAAAAK
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]