Click here to switch to the map view.

The map label for this gene is exoA [H]

Identifier: 66043485

GI number: 66043485

Start: 228597

End: 229376

Strand: Reverse

Name: exoA [H]

Synonym: Psyr_0215

Alternate gene names: 66043485

Gene position: 229376-228597 (Counterclockwise)

Preceding gene: 66043488

Following gene: 66043482

Centisome position: 3.76

GC content: 59.23

Gene sequence:

>780_bases
ATGCGGATCATCAGTGTGAACGTAAATGGCATTCAGGCTGCGGTCGAGCGTGGTTTGCTCAGCTGGCTGCAAGCTCAGAA
TGCCGACGTCATCTGCTTACAGGACACCCGCGCCTCCACCTTTGAACTGGACGATCCAGCCTTTCAGCTGGATGGTTACT
TCCTCTATGCCTGCGAAGCCGAGGTTCCCGCTCAGGGTGGTGTGGCGCTGTATTCGCGGTTGCAGCCGAAGGCGGTAATC
AGCGGCCTCGGCTTCGAGACAGCCGACCGCTACGGGCGCTACCTGCAAGCAGATTTCGACAAGGTCAGTATCGCTACCCT
GCTCTTTCCGTCGGGGATGAATGGCGATGAAGACCTGAATCAGAAATTCAAGCTCATGGACGATTTCGGCAAGTACATGG
ACAAGCAGCGCCGCAAGCGTCGCGAATACATCTACTGCGGATCGCTCTATGTGGCGCAGCAGAAGCTCGATATCAAGAAC
TGGCGCGACAGCCAGCAGTCGCCCGGCTTCCTCGCGCCCGAACGCGCCTGGATGGACGAAATCGTCGGCACCATGGGCTA
CGTCGATGCCCTGCGTGAAGTCAGCCGTGAAGGTGATCAGTACAGCTGGTGGCCGGATAACGAACAGGCCGAGATGCTGA
ACCTGGGTTGGCGCTTCGATTATCAGATCCTGACCCCTGGCCTGCGCCGCTTCGTGCGCAGCGCCCGTCTGCCACGCCAG
CCGCGCTTCTCGCAGCACGCGCCGTTGATCGTGGATTACGACTGGACCCTGACTATCTGA

Upstream 100 bases:

>100_bases
GCGTTGATACGCCTGCATGAAAAAAGCTCCGAATACCACGGATTTAGCTAATTAGGTAGAGCTCGGGTATCATACACGCA
CGTGATTTTTGGGGCCATTT

Downstream 100 bases:

>100_bases
TAGCCAGGCAAGGCAGCGAACACGAAAAAGCCGACTTGAAGTCGGCTTTTTGCGTTGCCTGAAGTGATGCTACTTGATCA
GGCGCAAGGTGAACGGATAG

Product: exodeoxyribonuclease III xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRIISVNVNGIQAAVERGLLSWLQAQNADVICLQDTRASTFELDDPAFQLDGYFLYACEAEVPAQGGVALYSRLQPKAVI
SGLGFETADRYGRYLQADFDKVSIATLLFPSGMNGDEDLNQKFKLMDDFGKYMDKQRRKRREYIYCGSLYVAQQKLDIKN
WRDSQQSPGFLAPERAWMDEIVGTMGYVDALREVSREGDQYSWWPDNEQAEMLNLGWRFDYQILTPGLRRFVRSARLPRQ
PRFSQHAPLIVDYDWTLTI

Sequences:

>Translated_259_residues
MRIISVNVNGIQAAVERGLLSWLQAQNADVICLQDTRASTFELDDPAFQLDGYFLYACEAEVPAQGGVALYSRLQPKAVI
SGLGFETADRYGRYLQADFDKVSIATLLFPSGMNGDEDLNQKFKLMDDFGKYMDKQRRKRREYIYCGSLYVAQQKLDIKN
WRDSQQSPGFLAPERAWMDEIVGTMGYVDALREVSREGDQYSWWPDNEQAEMLNLGWRFDYQILTPGLRRFVRSARLPRQ
PRFSQHAPLIVDYDWTLTI
>Mature_259_residues
MRIISVNVNGIQAAVERGLLSWLQAQNADVICLQDTRASTFELDDPAFQLDGYFLYACEAEVPAQGGVALYSRLQPKAVI
SGLGFETADRYGRYLQADFDKVSIATLLFPSGMNGDEDLNQKFKLMDDFGKYMDKQRRKRREYIYCGSLYVAQQKLDIKN
WRDSQQSPGFLAPERAWMDEIVGTMGYVDALREVSREGDQYSWWPDNEQAEMLNLGWRFDYQILTPGLRRFVRSARLPRQ
PRFSQHAPLIVDYDWTLTI

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=254, Percent_Identity=27.1653543307087, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI18375503, Length=254, Percent_Identity=27.1653543307087, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI18375501, Length=254, Percent_Identity=27.1653543307087, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1788046, Length=274, Percent_Identity=24.0875912408759, Blast_Score=63, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71989536, Length=259, Percent_Identity=24.7104247104247, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI221330655, Length=255, Percent_Identity=27.843137254902, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17136678, Length=236, Percent_Identity=29.2372881355932, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29869; Mature: 29869

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIISVNVNGIQAAVERGLLSWLQAQNADVICLQDTRASTFELDDPAFQLDGYFLYACEA
CEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCEEECCEEEEEECC
EVPAQGGVALYSRLQPKAVISGLGFETADRYGRYLQADFDKVSIATLLFPSGMNGDEDLN
CCCCCCCHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHH
QKFKLMDDFGKYMDKQRRKRREYIYCGSLYVAQQKLDIKNWRDSQQSPGFLAPERAWMDE
HHHHHHHHHHHHHHHHHHHHHCEEEECCEEEEHHHHCHHHCCCCCCCCCCCCCHHHHHHH
IVGTMGYVDALREVSREGDQYSWWPDNEQAEMLNLGWRFDYQILTPGLRRFVRSARLPRQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEECCCEEEHEEECHHHHHHHHHHCCCCC
PRFSQHAPLIVDYDWTLTI
CCCCCCCCEEEECCEEEEC
>Mature Secondary Structure
MRIISVNVNGIQAAVERGLLSWLQAQNADVICLQDTRASTFELDDPAFQLDGYFLYACEA
CEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCEEECCEEEEEECC
EVPAQGGVALYSRLQPKAVISGLGFETADRYGRYLQADFDKVSIATLLFPSGMNGDEDLN
CCCCCCCHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHH
QKFKLMDDFGKYMDKQRRKRREYIYCGSLYVAQQKLDIKNWRDSQQSPGFLAPERAWMDE
HHHHHHHHHHHHHHHHHHHHHCEEEECCEEEEHHHHCHHHCCCCCCCCCCCCCHHHHHHH
IVGTMGYVDALREVSREGDQYSWWPDNEQAEMLNLGWRFDYQILTPGLRRFVRSARLPRQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEECCCEEEHEEECHHHHHHHHHHCCCCC
PRFSQHAPLIVDYDWTLTI
CCCCCCCCEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]