Click here to switch to the map view.

The map label for this gene is gsiB [H]

Identifier: 66043427

GI number: 66043427

Start: 170483

End: 172120

Strand: Reverse

Name: gsiB [H]

Synonym: Psyr_0157

Alternate gene names: 66043427

Gene position: 172120-170483 (Counterclockwise)

Preceding gene: 66043428

Following gene: 66043426

Centisome position: 2.82

GC content: 61.54

Gene sequence:

>1638_bases
ATGACTGACAAAAAAAACGACTCCACGTTGATCACAGGTGAAGACAGCCTGCGGGTTTTCGAAGGACTCAATCGCGGTCT
GTCACGTCGCGATGCGCTACGCATGCTGGGCGTTGCAGGTGTGGTCGCGGCCGGCTCCACCAGCCTGTTCGGCGCGGCCG
GTAAGGTGTTTGCCGATGACGCGGATGCCCCTGTCAAAGGCAAGCCGGGCGGCCGTATCCGGGTCGCCGGCATGTCCAGT
TCTACCGCCGATACGCTGGATCCGGCCAAGGGCGCGCTGTCTACCGATTACGTGCGTCACTTCATGTTCTACAACGGCCT
GACCCGTTTCGACAGCCATCTGGTGCCGCACATGGAGCTGGCCGAGAAGATCGAAAGCGACAACGCCACGGTCTGGACCG
TGACCCTGCGCCAGGGCGTAACCTTCCACAACGGCAAGGCGCTGACCGCTGGCGACGTGGTGTTCTCCCTGTCGCGCCAC
AAGGACCCGGCCACCGGCTCGAAAGTCCTGCCATTGATGGCGCAGTTTTCCGAGATCAAAGCCACCGGCCCGCTGGAAGT
GCAGATCACCCTCAGCTCGCCAAACGCCGAGCTGCCGTCGATCCTCGCGGTTTCGCACCTGCTGATCGTGCCCGAAGGCA
CCACCGATTTCAGCAAAGGCATCGGTACCGGGCCGTTCACCGTCAAGGAATTCAACCCTGGCGTGCGCTCGGTCAGTGCG
CGCAACCCCAACTACTGGAAACCGGGCCTGCCGTACCTGGACGAAATCGAATTCATCGCCATCGCCGACGAACCCTCGCG
GGTCAACGCGCTGCTCTCCGGCGACGTACACATGATCAACGAGGTCAACCCGCGCTCCACCACGCGTATTGCCGCCAGCG
CCAAACACCGCGTGGTCGATGCACCGTCCGGCAACTACACCGACCTGATCATTCGTCAGGACCAGATGCCCGGCAAGAGC
CCGGAATTCACCGAGGCCATGAAGTACCTGCTGGACCGTGAACAGGTCAAATCGGCGGTGTTCCGTGGCTACGCCCGCGT
CGGTAACGACCATCCAATCTCGCCAGGCTCGCGCTACTTCAACGCGGACCTGCCACAGCGCGCCTACGACCCGGAAAAAG
CCAAATTCCTGCTCAAAAAAGCCGGCATGGAAAAGATCACCATGGCCGTGGCGGCATCGCCTGCTGCCACCGGCTCGGTG
GACATCGCCGTGCTGCTGCAACAGTCCGCCAAACAGGCCGGTCTGACCCTCAACGTCAACCGTCTGCCAAGCGACGGCTA
CTGGTCCAACCACTGGATGAAGCACCCGCTGAGCTTCGGCAACATCAACCCGCGCCCGAACGCCGACGTGATCTTCTCGC
AGTTCTTCCAGTCCACAGCGCCGTGGAATGAATCCGGCTGGAAAAACGACCAGTTCGACCAACTGCTGTTGCTGGCCCGT
GGCGAAACCGACGATGCCAAGCGCGGCAAAATGTATGGCGACATGCAAGCTCTGGTCAGCCAGAACTGCGGCATCGGCAT
CCCCGTGTTCATCAGCAACATCGACGGTGTGGACAAGCGTATCCAGGGCTATTCCAGCAACCCGCTGGGCGGCTTCATGG
GTTACATGTTCAGCGAGCAGGTGTGGCTGGACGCGTAA

Upstream 100 bases:

>100_bases
CCACTGAGTGTTTGTCTGACCGTCGTCGTGTGTGCTTGAAGGACGCTCCGGCCAACCGTGAGCAATTCGGAATTTCCCCT
TTTCGACAGGTAGAACTGAT

Downstream 100 bases:

>100_bases
ATGAAACCCTGAACCGAGCAGGAGGACGCAGATGAATAGCAACACACTCTGGTTGATCGTGCAGCGCTTCGGCGCAGCGA
TCGTGACGTTGTTGATCGTT

Product: twin-arginine translocation pathway signal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTDKKNDSTLITGEDSLRVFEGLNRGLSRRDALRMLGVAGVVAAGSTSLFGAAGKVFADDADAPVKGKPGGRIRVAGMSS
STADTLDPAKGALSTDYVRHFMFYNGLTRFDSHLVPHMELAEKIESDNATVWTVTLRQGVTFHNGKALTAGDVVFSLSRH
KDPATGSKVLPLMAQFSEIKATGPLEVQITLSSPNAELPSILAVSHLLIVPEGTTDFSKGIGTGPFTVKEFNPGVRSVSA
RNPNYWKPGLPYLDEIEFIAIADEPSRVNALLSGDVHMINEVNPRSTTRIAASAKHRVVDAPSGNYTDLIIRQDQMPGKS
PEFTEAMKYLLDREQVKSAVFRGYARVGNDHPISPGSRYFNADLPQRAYDPEKAKFLLKKAGMEKITMAVAASPAATGSV
DIAVLLQQSAKQAGLTLNVNRLPSDGYWSNHWMKHPLSFGNINPRPNADVIFSQFFQSTAPWNESGWKNDQFDQLLLLAR
GETDDAKRGKMYGDMQALVSQNCGIGIPVFISNIDGVDKRIQGYSSNPLGGFMGYMFSEQVWLDA

Sequences:

>Translated_545_residues
MTDKKNDSTLITGEDSLRVFEGLNRGLSRRDALRMLGVAGVVAAGSTSLFGAAGKVFADDADAPVKGKPGGRIRVAGMSS
STADTLDPAKGALSTDYVRHFMFYNGLTRFDSHLVPHMELAEKIESDNATVWTVTLRQGVTFHNGKALTAGDVVFSLSRH
KDPATGSKVLPLMAQFSEIKATGPLEVQITLSSPNAELPSILAVSHLLIVPEGTTDFSKGIGTGPFTVKEFNPGVRSVSA
RNPNYWKPGLPYLDEIEFIAIADEPSRVNALLSGDVHMINEVNPRSTTRIAASAKHRVVDAPSGNYTDLIIRQDQMPGKS
PEFTEAMKYLLDREQVKSAVFRGYARVGNDHPISPGSRYFNADLPQRAYDPEKAKFLLKKAGMEKITMAVAASPAATGSV
DIAVLLQQSAKQAGLTLNVNRLPSDGYWSNHWMKHPLSFGNINPRPNADVIFSQFFQSTAPWNESGWKNDQFDQLLLLAR
GETDDAKRGKMYGDMQALVSQNCGIGIPVFISNIDGVDKRIQGYSSNPLGGFMGYMFSEQVWLDA
>Mature_544_residues
TDKKNDSTLITGEDSLRVFEGLNRGLSRRDALRMLGVAGVVAAGSTSLFGAAGKVFADDADAPVKGKPGGRIRVAGMSSS
TADTLDPAKGALSTDYVRHFMFYNGLTRFDSHLVPHMELAEKIESDNATVWTVTLRQGVTFHNGKALTAGDVVFSLSRHK
DPATGSKVLPLMAQFSEIKATGPLEVQITLSSPNAELPSILAVSHLLIVPEGTTDFSKGIGTGPFTVKEFNPGVRSVSAR
NPNYWKPGLPYLDEIEFIAIADEPSRVNALLSGDVHMINEVNPRSTTRIAASAKHRVVDAPSGNYTDLIIRQDQMPGKSP
EFTEAMKYLLDREQVKSAVFRGYARVGNDHPISPGSRYFNADLPQRAYDPEKAKFLLKKAGMEKITMAVAASPAATGSVD
IAVLLQQSAKQAGLTLNVNRLPSDGYWSNHWMKHPLSFGNINPRPNADVIFSQFFQSTAPWNESGWKNDQFDQLLLLARG
ETDDAKRGKMYGDMQALVSQNCGIGIPVFISNIDGVDKRIQGYSSNPLGGFMGYMFSEQVWLDA

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=470, Percent_Identity=26.3829787234043, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789966, Length=474, Percent_Identity=25.5274261603376, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1787762, Length=477, Percent_Identity=23.8993710691824, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1789887, Length=296, Percent_Identity=28.7162162162162, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787495, Length=540, Percent_Identity=23.5185185185185, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787551, Length=543, Percent_Identity=22.6519337016575, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59247; Mature: 59116

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKNDSTLITGEDSLRVFEGLNRGLSRRDALRMLGVAGVVAAGSTSLFGAAGKVFADD
CCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECC
ADAPVKGKPGGRIRVAGMSSSTADTLDPAKGALSTDYVRHFMFYNGLTRFDSHLVPHMEL
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCHHHH
AEKIESDNATVWTVTLRQGVTFHNGKALTAGDVVFSLSRHKDPATGSKVLPLMAQFSEIK
HHHHCCCCCEEEEEEEECCCEEECCCEEEHHHHEEEHHCCCCCCCCCHHHHHHHHHHHHC
ATGPLEVQITLSSPNAELPSILAVSHLLIVPEGTTDFSKGIGTGPFTVKEFNPGVRSVSA
CCCCEEEEEEECCCCCCCCHHHEEEEEEEEECCCCHHHHCCCCCCEEEECCCCCHHEECC
RNPNYWKPGLPYLDEIEFIAIADEPSRVNALLSGDVHMINEVNPRSTTRIAASAKHRVVD
CCCCCCCCCCCCCCCEEEEEEECCCHHEEEEECCCEEEEECCCCCCHHEEECCCCCEEEE
APSGNYTDLIIRQDQMPGKSPEFTEAMKYLLDREQVKSAVFRGYARVGNDHPISPGSRYF
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
NADLPQRAYDPEKAKFLLKKAGMEKITMAVAASPAATGSVDIAVLLQQSAKQAGLTLNVN
CCCCCCCCCCHHHHHHHHHHCCHHHEEEEEECCCCCCCCEEHHHHHHHHHHHCCCEEEEE
RLPSDGYWSNHWMKHPLSFGNINPRPNADVIFSQFFQSTAPWNESGWKNDQFDQLLLLAR
CCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
GETDDAKRGKMYGDMQALVSQNCGIGIPVFISNIDGVDKRIQGYSSNPLGGFMGYMFSEQ
CCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHHCCC
VWLDA
EEECC
>Mature Secondary Structure 
TDKKNDSTLITGEDSLRVFEGLNRGLSRRDALRMLGVAGVVAAGSTSLFGAAGKVFADD
CCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECC
ADAPVKGKPGGRIRVAGMSSSTADTLDPAKGALSTDYVRHFMFYNGLTRFDSHLVPHMEL
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCHHHH
AEKIESDNATVWTVTLRQGVTFHNGKALTAGDVVFSLSRHKDPATGSKVLPLMAQFSEIK
HHHHCCCCCEEEEEEEECCCEEECCCEEEHHHHEEEHHCCCCCCCCCHHHHHHHHHHHHC
ATGPLEVQITLSSPNAELPSILAVSHLLIVPEGTTDFSKGIGTGPFTVKEFNPGVRSVSA
CCCCEEEEEEECCCCCCCCHHHEEEEEEEEECCCCHHHHCCCCCCEEEECCCCCHHEECC
RNPNYWKPGLPYLDEIEFIAIADEPSRVNALLSGDVHMINEVNPRSTTRIAASAKHRVVD
CCCCCCCCCCCCCCCEEEEEEECCCHHEEEEECCCEEEEECCCCCCHHEEECCCCCEEEE
APSGNYTDLIIRQDQMPGKSPEFTEAMKYLLDREQVKSAVFRGYARVGNDHPISPGSRYF
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
NADLPQRAYDPEKAKFLLKKAGMEKITMAVAASPAATGSVDIAVLLQQSAKQAGLTLNVN
CCCCCCCCCCHHHHHHHHHHCCHHHEEEEEECCCCCCCCEEHHHHHHHHHHHCCCEEEEE
RLPSDGYWSNHWMKHPLSFGNINPRPNADVIFSQFFQSTAPWNESGWKNDQFDQLLLLAR
CCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
GETDDAKRGKMYGDMQALVSQNCGIGIPVFISNIDGVDKRIQGYSSNPLGGFMGYMFSEQ
CCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHHCCC
VWLDA
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA