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The map label for this gene is trpI [H]

Identifier: 66043306

GI number: 66043306

Start: 39307

End: 40263

Strand: Direct

Name: trpI [H]

Synonym: Psyr_0035

Alternate gene names: 66043306

Gene position: 39307-40263 (Clockwise)

Preceding gene: 66043302

Following gene: 66043308

Centisome position: 0.65

GC content: 61.34

Gene sequence:

>957_bases
ATGAAAACCGATAAGATCGCTGCAACCTGTCAGGAAAACTCACAGATCATGAGCCGCGACCTCCCTCCCCTCAATGCATT
GCGCGCCTTTGAAAGCACTGCCCGGCTTGGCAGTGTCAGTCAGGCGGCCGAACAGCTGAACGTGACGCATGGCGCCGTCA
GTCGACAATTGAAAGTGCTTGAAGAACACTTGGGTACAAGCCTGTTCAGCAAGGACGGACGAGGCTTGAAACTCACAGAT
GCCGGTATTCGGCTGCGCGATGTCAGTGGCGATGCGTTTGATCGGTTGCGTGGTGTATGTGCAGAGCTGAGCAGGGGCAG
CGCAGACGCGCCGTTCGTGTTGGGGTGCTCCGGCAGCCTGCTGGCGCGCTGGTTCATCCCGCGCTTGAGTCGCCTGAATG
CCGAGCTGCCGGACCTGCGTCTGCACTTGTCGGCAGGCGAAGGTGATCTTGACCCGCGTCGACCAGGTCTTGATGCCCTG
CTGATATTCGCCGAGCCGCCATGGCCGGCAGACATGCAGGTCTTCGAACTGGCAAGCGAGCGGATCGGACCGGTGTTGAG
CCCGCGCTTCGCGCGCTTCGAGCGTTTGTGCGATGCACCTGCTGAGGCGTTGCTGGAAGAGTCGCTGCTGCAAACCACAT
CGCGTCCGCAGGCGTGGCCAAGCTGGGCGCAGCAGAACGGCATCGACCCGCAGTCGTTGCGTTACGGGCAGGGGTTCGAG
CATTTGTATTATTTGCTGGAGGCGGCCGTGGCAGGCCTTGGCATCGCGATTGCGCCGGAACCTCTGGTCATCGATGACCT
GAAAGCCGGTCGCCTGGCTGCGCCATGGGGCTTCAGTGAAACCCCGGCGCAGCTGGCGCTTTGGGTACCCAGACGTGCGG
CCGATGGACGGGCGCAGCAGCTTGCTCAGTGGTTGAAAAATGAGCTGAAGCGGTCCGGGGAACCGATCAGAAACTGA

Upstream 100 bases:

>100_bases
GCGGAAATTGGTCTGGGTCATGAAGTGCTCCAGTTGCAGTATTGGGCGAATAGGTTGAGCCGTATTGGCAATGGGTCCAC
TTTAACCAGCGCAAGGCCGG

Downstream 100 bases:

>100_bases
TCAGTTACCGCGCTTGAACAGCATGTAGGCAGCGATCAGGCCGATGGCACCTACCGCAACGCCAGCGGTGGTCCAAGGGT
GTTCCTGAGCGTAGTCACGG

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKTDKIAATCQENSQIMSRDLPPLNALRAFESTARLGSVSQAAEQLNVTHGAVSRQLKVLEEHLGTSLFSKDGRGLKLTD
AGIRLRDVSGDAFDRLRGVCAELSRGSADAPFVLGCSGSLLARWFIPRLSRLNAELPDLRLHLSAGEGDLDPRRPGLDAL
LIFAEPPWPADMQVFELASERIGPVLSPRFARFERLCDAPAEALLEESLLQTTSRPQAWPSWAQQNGIDPQSLRYGQGFE
HLYYLLEAAVAGLGIAIAPEPLVIDDLKAGRLAAPWGFSETPAQLALWVPRRAADGRAQQLAQWLKNELKRSGEPIRN

Sequences:

>Translated_318_residues
MKTDKIAATCQENSQIMSRDLPPLNALRAFESTARLGSVSQAAEQLNVTHGAVSRQLKVLEEHLGTSLFSKDGRGLKLTD
AGIRLRDVSGDAFDRLRGVCAELSRGSADAPFVLGCSGSLLARWFIPRLSRLNAELPDLRLHLSAGEGDLDPRRPGLDAL
LIFAEPPWPADMQVFELASERIGPVLSPRFARFERLCDAPAEALLEESLLQTTSRPQAWPSWAQQNGIDPQSLRYGQGFE
HLYYLLEAAVAGLGIAIAPEPLVIDDLKAGRLAAPWGFSETPAQLALWVPRRAADGRAQQLAQWLKNELKRSGEPIRN
>Mature_318_residues
MKTDKIAATCQENSQIMSRDLPPLNALRAFESTARLGSVSQAAEQLNVTHGAVSRQLKVLEEHLGTSLFSKDGRGLKLTD
AGIRLRDVSGDAFDRLRGVCAELSRGSADAPFVLGCSGSLLARWFIPRLSRLNAELPDLRLHLSAGEGDLDPRRPGLDAL
LIFAEPPWPADMQVFELASERIGPVLSPRFARFERLCDAPAEALLEESLLQTTSRPQAWPSWAQQNGIDPQSLRYGQGFE
HLYYLLEAAVAGLGIAIAPEPLVIDDLKAGRLAAPWGFSETPAQLALWVPRRAADGRAQQLAQWLKNELKRSGEPIRN

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=261, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=29.4520547945205, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788706, Length=261, Percent_Identity=29.1187739463602, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34830; Mature: 34830

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDKIAATCQENSQIMSRDLPPLNALRAFESTARLGSVSQAAEQLNVTHGAVSRQLKVL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEHLGTSLFSKDGRGLKLTDAGIRLRDVSGDAFDRLRGVCAELSRGSADAPFVLGCSGSL
HHHHCHHHHCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHH
LARWFIPRLSRLNAELPDLRLHLSAGEGDLDPRRPGLDALLIFAEPPWPADMQVFELASE
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
RIGPVLSPRFARFERLCDAPAEALLEESLLQTTSRPQAWPSWAQQNGIDPQSLRYGQGFE
HHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHCCCCHH
HLYYLLEAAVAGLGIAIAPEPLVIDDLKAGRLAAPWGFSETPAQLALWVPRRAADGRAQQ
HHHHHHHHHHHHCCEEECCCCEEEECCCCCCEECCCCCCCCCHHEEEECCCCCCCCHHHH
LAQWLKNELKRSGEPIRN
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKTDKIAATCQENSQIMSRDLPPLNALRAFESTARLGSVSQAAEQLNVTHGAVSRQLKVL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEHLGTSLFSKDGRGLKLTDAGIRLRDVSGDAFDRLRGVCAELSRGSADAPFVLGCSGSL
HHHHCHHHHCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHH
LARWFIPRLSRLNAELPDLRLHLSAGEGDLDPRRPGLDALLIFAEPPWPADMQVFELASE
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
RIGPVLSPRFARFERLCDAPAEALLEESLLQTTSRPQAWPSWAQQNGIDPQSLRYGQGFE
HHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHCCCCHH
HLYYLLEAAVAGLGIAIAPEPLVIDDLKAGRLAAPWGFSETPAQLALWVPRRAADGRAQQ
HHHHHHHHHHHHCCEEECCCCEEEECCCCCCEECCCCCCCCCHHEEEECCCCCCCCHHHH
LAQWLKNELKRSGEPIRN
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8423001 [H]