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The map label for this gene is smf [H]

Identifier: 66043292

GI number: 66043292

Start: 24821

End: 25939

Strand: Direct

Name: smf [H]

Synonym: Psyr_0021

Alternate gene names: 66043292

Gene position: 24821-25939 (Clockwise)

Preceding gene: 66043291

Following gene: 66043293

Centisome position: 0.41

GC content: 64.16

Gene sequence:

>1119_bases
ATGCCATTGTTCGAAAATGCCCCCTCTTCACCCGCAGAGCTGGAAGCGCGTTTGCGCATGCACCGCTTGCCCGAGATAGG
GCCGAAACGCTTTTCCAGACTGATCGAAGCCTTCGGTTCGGCCTCGTCGGCGCTCAGTGCGCCGGCCAGCGCATGGCGTG
CGCTGGGTATTCCTGGTGCCTGTGCCGAGGCGCGTCGTGACCCCTCCGTACGTGATGGCGCCAGTGCCGCATTGGCCTGG
CTGGAGCGTCCGGCCCAGCATTTGCTGATGTGGGACGACCCTGGCTACCCGGCATTGCTGGCGCAGATCGCTGATCCACC
GCCGCTGATCTTCATTGCCGGCGACCCTTCGATCCTGGAGCGTCCGCAGTTGGGAATGGTCGGCAGTCGTCGCGCTTCAC
GACCCGGGCTGGACACCGCCAGCGCTTTTGCGCGCAGCCTGGCGGCTGCCGGTTTTGTGATCACCAGTGGCCTGGCGCGG
GGTATTGACGGCGCCGCGCATCAGGGCGCATTGGATGTCGGGGGGCTTACAATCGGCGTGCTTGGCACCGGGCTCGAAAA
ACTTTATCCACAGCAGCACCGCGCCCTCGCCGCGCAGATCGCGGCACAGGGCGGCGCAGTGATTTCCGAGTTCCCGCTGG
ACGCCGCAGCACAGGCCAGCAACTTCCCGCGGCGTAACCGAATCATCAGCGGCCTGTCGCTGGGCATACTGGTGGTCGAG
GCCAGTGTCGCCAGCGGCTCGTTGATTACCGCGCGACTGGCCGCCGAGCATGGACGAGAGGTGTATGCGATACCTGGCTC
GATTCATCACCCTGGCGCCAAGGGCTGTCATCAGTTGATACGTGACGGCGCGACACTGGTCGAGACCGTCGAGCACATTC
TTGAAGGCTTGCAGCACTGGAAACGTGTTACGCCCGACATCGATCCTGTCACGCCGGCGAAGCCTGTCCCTTGCGATCAT
CCTTTATTGGCGCTGCTGCACGCCGCACCGCACACCAGCGAGGGGCTGTCGGTGTCATGCGGCTGGCCATTGCCCAAGGT
ATTGGCAGGCCTGACCGAGCTGGAACTGGACGGACGCATAAGCTGTGAAGCAGGACGATGGTTCGCCAGAGCGCCCTGA

Upstream 100 bases:

>100_bases
GAATTGTGCGACGAACCAGACACTTATCAAATTGTTGTCAACAGAGTTATCCACAGGTTTGTGCAGCCCTCGGGATGCAC
GATGGATCAGGGACGATCTC

Downstream 100 bases:

>100_bases
GGTTAAACTGCCGCCAGGATTCAGTGGAGAGACAAACAATGGTCAGCAGTTGGCGAGTGCAACAAGCCGCTCAGGATATT
CGAGCCGGTGCGGTGATTGC

Product: SMF protein

Products: NA

Alternate protein names: DNA-processing chain A [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MPLFENAPSSPAELEARLRMHRLPEIGPKRFSRLIEAFGSASSALSAPASAWRALGIPGACAEARRDPSVRDGASAALAW
LERPAQHLLMWDDPGYPALLAQIADPPPLIFIAGDPSILERPQLGMVGSRRASRPGLDTASAFARSLAAAGFVITSGLAR
GIDGAAHQGALDVGGLTIGVLGTGLEKLYPQQHRALAAQIAAQGGAVISEFPLDAAAQASNFPRRNRIISGLSLGILVVE
ASVASGSLITARLAAEHGREVYAIPGSIHHPGAKGCHQLIRDGATLVETVEHILEGLQHWKRVTPDIDPVTPAKPVPCDH
PLLALLHAAPHTSEGLSVSCGWPLPKVLAGLTELELDGRISCEAGRWFARAP

Sequences:

>Translated_372_residues
MPLFENAPSSPAELEARLRMHRLPEIGPKRFSRLIEAFGSASSALSAPASAWRALGIPGACAEARRDPSVRDGASAALAW
LERPAQHLLMWDDPGYPALLAQIADPPPLIFIAGDPSILERPQLGMVGSRRASRPGLDTASAFARSLAAAGFVITSGLAR
GIDGAAHQGALDVGGLTIGVLGTGLEKLYPQQHRALAAQIAAQGGAVISEFPLDAAAQASNFPRRNRIISGLSLGILVVE
ASVASGSLITARLAAEHGREVYAIPGSIHHPGAKGCHQLIRDGATLVETVEHILEGLQHWKRVTPDIDPVTPAKPVPCDH
PLLALLHAAPHTSEGLSVSCGWPLPKVLAGLTELELDGRISCEAGRWFARAP
>Mature_371_residues
PLFENAPSSPAELEARLRMHRLPEIGPKRFSRLIEAFGSASSALSAPASAWRALGIPGACAEARRDPSVRDGASAALAWL
ERPAQHLLMWDDPGYPALLAQIADPPPLIFIAGDPSILERPQLGMVGSRRASRPGLDTASAFARSLAAAGFVITSGLARG
IDGAAHQGALDVGGLTIGVLGTGLEKLYPQQHRALAAQIAAQGGAVISEFPLDAAAQASNFPRRNRIISGLSLGILVVEA
SVASGSLITARLAAEHGREVYAIPGSIHHPGAKGCHQLIRDGATLVETVEHILEGLQHWKRVTPDIDPVTPAKPVPCDHP
LLALLHAAPHTSEGLSVSCGWPLPKVLAGLTELELDGRISCEAGRWFARAP

Specific function: Essential for efficient chromosomal DNA transformation but not required for plasmid transformation [H]

COG id: COG0758

COG function: function code LU; Predicted Rossmann fold nucleotide-binding protein involved in DNA uptake

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smf family [H]

Homologues:

Organism=Escherichia coli, GI48994932, Length=303, Percent_Identity=46.8646864686469, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003488 [H]

Pfam domain/function: PF02481 DNA_processg_A [H]

EC number: NA

Molecular weight: Translated: 38992; Mature: 38861

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLFENAPSSPAELEARLRMHRLPEIGPKRFSRLIEAFGSASSALSAPASAWRALGIPGA
CCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCCCH
CAEARRDPSVRDGASAALAWLERPAQHLLMWDDPGYPALLAQIADPPPLIFIAGDPSILE
HHHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHCCCCCEEEECCCCHHHC
RPQLGMVGSRRASRPGLDTASAFARSLAAAGFVITSGLARGIDGAAHQGALDVGGLTIGV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEECCCEEEEH
LGTGLEKLYPQQHRALAAQIAAQGGAVISEFPLDAAAQASNFPRRNRIISGLSLGILVVE
HHHCHHHHCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHCCCCHHHHHHHHHHEEEEEEE
ASVASGSLITARLAAEHGREVYAIPGSIHHPGAKGCHQLIRDGATLVETVEHILEGLQHW
ECCCCCCHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KRVTPDIDPVTPAKPVPCDHPLLALLHAAPHTSEGLSVSCGWPLPKVLAGLTELELDGRI
HHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCHHHHHHCHHHCCCCCEE
SCEAGRWFARAP
EECCCCEECCCC
>Mature Secondary Structure 
PLFENAPSSPAELEARLRMHRLPEIGPKRFSRLIEAFGSASSALSAPASAWRALGIPGA
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCCCH
CAEARRDPSVRDGASAALAWLERPAQHLLMWDDPGYPALLAQIADPPPLIFIAGDPSILE
HHHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHCCCCCEEEECCCCHHHC
RPQLGMVGSRRASRPGLDTASAFARSLAAAGFVITSGLARGIDGAAHQGALDVGGLTIGV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEECCCEEEEH
LGTGLEKLYPQQHRALAAQIAAQGGAVISEFPLDAAAQASNFPRRNRIISGLSLGILVVE
HHHCHHHHCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHCCCCHHHHHHHHHHEEEEEEE
ASVASGSLITARLAAEHGREVYAIPGSIHHPGAKGCHQLIRDGATLVETVEHILEGLQHW
ECCCCCCHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KRVTPDIDPVTPAKPVPCDHPLLALLHAAPHTSEGLSVSCGWPLPKVLAGLTELELDGRI
HHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCHHHHHHCHHHCCCCCEE
SCEAGRWFARAP
EECCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7768823; 7542800 [H]