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The map label for this gene is def [H]

Identifier: 66043290

GI number: 66043290

Start: 23040

End: 23546

Strand: Reverse

Name: def [H]

Synonym: Psyr_0019

Alternate gene names: 66043290

Gene position: 23546-23040 (Counterclockwise)

Preceding gene: 66043294

Following gene: 66043289

Centisome position: 0.39

GC content: 55.62

Gene sequence:

>507_bases
ATGGCCATTCTAAACATCCTCGAATTTCCCGATTCGCGCCTGCGCACCATCGCCAAACCTGTGGCGATGGTAGACGACGG
CATTCGCCAGTTGGTCGACGACATGTTTGAAACCATGTACGAAGCGCCAGGTATTGGCCTGGCCGCGACTCAGGTCAACG
TCCACAAGCGCGTGGTCGTCATGGACCTGAGCGAAGACCGTAGCGAACCGATGGTCTTCATCAATCCTGAAATCGAAAAA
CTGACCGATGAAATGGATCAGTATCAGGAAGGCTGCCTGTCGGTGCCGGGCTTCTACGAGAACGTCGACCGTCCGCAAAA
GGTCCGCGTCAAGGCGCTGGACCGTGACGGCAAGCCTTATGAACTGGTTGCCGAGGGCCTGCTCGCCATCTGTATCCAGC
ATGAATGCGATCACCTGAACGGCAAGCTGTTCGTCGATTACCTGTCCAACCTCAAGCGTGACCGGATCAAGAAGAAGCTC
GAAAAGCAGCACAAGCTCAACGCCTGA

Upstream 100 bases:

>100_bases
CTCACGCGAACCCTAGAGTCCTGTCCTGGGCTGCCTGCCTTGAACTCCCGGGCCCGGCACCCAACATTGACTTTACCTTA
ACAGTGCAGCTCTTACGTAT

Downstream 100 bases:

>100_bases
TCCGTCGTTACCCCAAAAGGCCTGAACAGGCCTTTTTTTATAATGAGAACTCCATGACTGAGCCACTGCGCATCGTCTTC
GCCGGCACCCCGGAATTCGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MAILNILEFPDSRLRTIAKPVAMVDDGIRQLVDDMFETMYEAPGIGLAATQVNVHKRVVVMDLSEDRSEPMVFINPEIEK
LTDEMDQYQEGCLSVPGFYENVDRPQKVRVKALDRDGKPYELVAEGLLAICIQHECDHLNGKLFVDYLSNLKRDRIKKKL
EKQHKLNA

Sequences:

>Translated_168_residues
MAILNILEFPDSRLRTIAKPVAMVDDGIRQLVDDMFETMYEAPGIGLAATQVNVHKRVVVMDLSEDRSEPMVFINPEIEK
LTDEMDQYQEGCLSVPGFYENVDRPQKVRVKALDRDGKPYELVAEGLLAICIQHECDHLNGKLFVDYLSNLKRDRIKKKL
EKQHKLNA
>Mature_167_residues
AILNILEFPDSRLRTIAKPVAMVDDGIRQLVDDMFETMYEAPGIGLAATQVNVHKRVVVMDLSEDRSEPMVFINPEIEKL
TDEMDQYQEGCLSVPGFYENVDRPQKVRVKALDRDGKPYELVAEGLLAICIQHECDHLNGKLFVDYLSNLKRDRIKKKLE
KQHKLNA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=165, Percent_Identity=31.5151515151515, Blast_Score=81, Evalue=4e-16,
Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=57.7380952380952, Blast_Score=199, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24645728, Length=137, Percent_Identity=35.036496350365, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24645726, Length=142, Percent_Identity=30.9859154929577, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19244; Mature: 19112

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILNILEFPDSRLRTIAKPVAMVDDGIRQLVDDMFETMYEAPGIGLAATQVNVHKRVVV
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEE
MDLSEDRSEPMVFINPEIEKLTDEMDQYQEGCLSVPGFYENVDRPQKVRVKALDRDGKPY
EECCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHEECCCCCCH
ELVAEGLLAICIQHECDHLNGKLFVDYLSNLKRDRIKKKLEKQHKLNA
HHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AILNILEFPDSRLRTIAKPVAMVDDGIRQLVDDMFETMYEAPGIGLAATQVNVHKRVVV
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEE
MDLSEDRSEPMVFINPEIEKLTDEMDQYQEGCLSVPGFYENVDRPQKVRVKALDRDGKPY
EECCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHEECCCCCCH
ELVAEGLLAICIQHECDHLNGKLFVDYLSNLKRDRIKKKLEKQHKLNA
HHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]