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The map label for this gene is tag [H]

Identifier: 66043284

GI number: 66043284

Start: 14995

End: 15546

Strand: Direct

Name: tag [H]

Synonym: Psyr_0013

Alternate gene names: 66043284

Gene position: 14995-15546 (Clockwise)

Preceding gene: 66043279

Following gene: 66043285

Centisome position: 0.25

GC content: 60.14

Gene sequence:

>552_bases
ATGACACGCTGCTTTTGGTGCAACGAAGACCCCCTGTACATCGCATATCACGATCAGGAGTGGGGAGTGCCGCTGCGCGA
TGCGCAGAAGCTCTTCGAGTTGCTTTTGCTCGAAGGGTTCCAGGCCGGGCTGTCGTGGATCACGATTCTCAAGCGGCGCG
CGCGTTATCGCGAAGTACTGCACGGCTTTGACGTCGAGCGCCTGGCGCAGCTGAGCGATGCCGAAATAGAGGCGCTGATG
CTTGATCCGAGCATCATCCGCAACCGCCTCAAGCTCAAGGCGGTGCGCACCAATGCGCGGGCCTGGCTGGCACTGGAAGA
CCCGGTCGCGCTGCTCTGGTCGTTTGTCGGCGGTACTCCGAAGATCAATCACTTCAAGGATCGCAGCGAAGTCCCGGCGA
TGACCGCCGAGGCCGAAGCGATGAGCAAGGCATTGAAAAAGGCCGGTTTCACCTTTGTCGGGCCGACCATCTGTTACGCC
TACATGCAGGCCAGCGGCATGGTCATGGACCACACTGTCGATTGCGATCGGTATGCGATCCTGAGCCGCTGA

Upstream 100 bases:

>100_bases
GCTGGCGTAGGCTGGCTCACAAATTTCACCTGTGCTGGGCTGCGATTCAAAGAGCGGGAGTATACCCGATTCGGCCGCAC
GTCCACTCCTTGGGAGCCCT

Downstream 100 bases:

>100_bases
CGGTTACACTACCCGCCTCGAGAATCTGGGAGTCATCTGTGGATAAGTTCAAAGGCGCCGTAATGGTCGGGGCGTTGCGA
TTGTTTGCGCTGTTGCCATG

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MTRCFWCNEDPLYIAYHDQEWGVPLRDAQKLFELLLLEGFQAGLSWITILKRRARYREVLHGFDVERLAQLSDAEIEALM
LDPSIIRNRLKLKAVRTNARAWLALEDPVALLWSFVGGTPKINHFKDRSEVPAMTAEAEAMSKALKKAGFTFVGPTICYA
YMQASGMVMDHTVDCDRYAILSR

Sequences:

>Translated_183_residues
MTRCFWCNEDPLYIAYHDQEWGVPLRDAQKLFELLLLEGFQAGLSWITILKRRARYREVLHGFDVERLAQLSDAEIEALM
LDPSIIRNRLKLKAVRTNARAWLALEDPVALLWSFVGGTPKINHFKDRSEVPAMTAEAEAMSKALKKAGFTFVGPTICYA
YMQASGMVMDHTVDCDRYAILSR
>Mature_182_residues
TRCFWCNEDPLYIAYHDQEWGVPLRDAQKLFELLLLEGFQAGLSWITILKRRARYREVLHGFDVERLAQLSDAEIEALML
DPSIIRNRLKLKAVRTNARAWLALEDPVALLWSFVGGTPKINHFKDRSEVPAMTAEAEAMSKALKKAGFTFVGPTICYAY
MQASGMVMDHTVDCDRYAILSR

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=182, Percent_Identity=52.1978021978022, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 20929; Mature: 20797

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRCFWCNEDPLYIAYHDQEWGVPLRDAQKLFELLLLEGFQAGLSWITILKRRARYREVL
CCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGFDVERLAQLSDAEIEALMLDPSIIRNRLKLKAVRTNARAWLALEDPVALLWSFVGGTP
CCCCHHHHHHCCCCCCEEEEECHHHHHHHHHHHHEECCCEEEEEECCHHHHHHHHHCCCC
KINHFKDRSEVPAMTAEAEAMSKALKKAGFTFVGPTICYAYMQASGMVMDHTVDCDRYAI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCEEEEEECCCCCCCC
LSR
CCC
>Mature Secondary Structure 
TRCFWCNEDPLYIAYHDQEWGVPLRDAQKLFELLLLEGFQAGLSWITILKRRARYREVL
CEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGFDVERLAQLSDAEIEALMLDPSIIRNRLKLKAVRTNARAWLALEDPVALLWSFVGGTP
CCCCHHHHHHCCCCCCEEEEECHHHHHHHHHHHHEECCCEEEEEECCHHHHHHHHHCCCC
KINHFKDRSEVPAMTAEAEAMSKALKKAGFTFVGPTICYAYMQASGMVMDHTVDCDRYAI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCEEEEEECCCCCCCC
LSR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]