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The map label for this gene is dnaN [H]

Identifier: 66043273

GI number: 66043273

Start: 1575

End: 2678

Strand: Direct

Name: dnaN [H]

Synonym: Psyr_0002

Alternate gene names: 66043273

Gene position: 1575-2678 (Clockwise)

Preceding gene: 66043272

Following gene: 66043274

Centisome position: 0.03

GC content: 57.7

Gene sequence:

>1104_bases
ATGCATTTCACCATTCAACGCGAAGCCCTGTTGAAACCCCTGCAATTGGTCGCCGGCGTCGTCGAACGCCGCCAGACCTT
GCCGGTTCTTTCCAATGTGCTCCTGGTCGTCGAAGGTCAGCAACTGTCGCTGACCGGTACGGATCTGGAAGTCGAACTGG
TTGGCCGCGTCCAGCTGGAAGAGCCCGCCGAACCTGGAGAAATCACGGTTCCTGCGCGCAAGCTCATGGACATTTGCAAA
AGCCTGTCCAATGACGCGCTGATCGACATCAAGCTCGATGACTCGAAACTGATCGTCAAGGCCGGTCGTAGCCGGTTCAC
CCTGTCGACTCTGCCTGCCAATGATTTCCCTACCGTTGAAGAAGGCCCGGGTTCGCTGACGTTCACTCTGGTGCAGAGCA
AACTGCGCCGCTTGATCGAGCGCACCAGCTTTGCGATGGCCCAGCAGGACGTTCGGTACTACCTCAACGGCATGCTGCTT
GAAGTGAGTGCCGGTATCTTGCGTGCGGTTGCCACCGACGGCCACCGCCTGGCCATGTGCTCGATGTCTGCCGATATCGA
ACACGCCGATCGCCATCAGGTGATCGTTCCACGCAAAGGTATTCTTGAAATGGCGCGCCTGCTGACCGAACAGGACGGCA
CGGTCAGTATCGTTCTGGGTCAGCATCACATCCGTGCGACGACTGGCGAATTCACCTTCACCTCCAAACTGGTCGATGGC
AAGTTCCCGGATTACGAGCGTGTGCTGCCCAAAGGTGGTGACAAGCTGGTGCTCGGTGATCGTCAGGCACTGCGTGAGGC
GTTCAGCCGCACGGCGATTCTGTCCAACGAGAAGTACCGCGGCATCCGTCTGCAACTGGCCAGCGGTCAATTGAAGATTC
AGGCCAACAACCCTGAGCAGGAAGAAGCAGAAGAGGAAATCAGCGTCGAGTACAACGGTGACTCGTTGGAGATTGGTTTC
AACGTGAGCTACTTGCTCGACGTGCTGGGCGTCATGACCACCGAACAGGTGCGCCTGATTCTGTCCGACTCCAATAGCAG
CGCCCTGGTGCAAGAGTCCGATAACGACGACTCCGCCTACGTTGTCATGCCGATGCGTCTGTAA

Upstream 100 bases:

>100_bases
AGGAATCCGATGCGGATATCCGCGAGGACTACAAGAACCTGCTGCGCACTCTGACTACGTGATAACGCCAGTGCAGCTCA
TCAAGGCAAGGGACTAGACC

Downstream 100 bases:

>100_bases
CTTCATGTCCAGCGCAACCTAGATGTCTCTTAGTCGCGTCTCGGTCACCGGGGTGCGCAATCTGCACCCGGTGACCCTCT
CCCCCTCCCCGCGTATCAAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MHFTIQREALLKPLQLVAGVVERRQTLPVLSNVLLVVEGQQLSLTGTDLEVELVGRVQLEEPAEPGEITVPARKLMDICK
SLSNDALIDIKLDDSKLIVKAGRSRFTLSTLPANDFPTVEEGPGSLTFTLVQSKLRRLIERTSFAMAQQDVRYYLNGMLL
EVSAGILRAVATDGHRLAMCSMSADIEHADRHQVIVPRKGILEMARLLTEQDGTVSIVLGQHHIRATTGEFTFTSKLVDG
KFPDYERVLPKGGDKLVLGDRQALREAFSRTAILSNEKYRGIRLQLASGQLKIQANNPEQEEAEEEISVEYNGDSLEIGF
NVSYLLDVLGVMTTEQVRLILSDSNSSALVQESDNDDSAYVVMPMRL

Sequences:

>Translated_367_residues
MHFTIQREALLKPLQLVAGVVERRQTLPVLSNVLLVVEGQQLSLTGTDLEVELVGRVQLEEPAEPGEITVPARKLMDICK
SLSNDALIDIKLDDSKLIVKAGRSRFTLSTLPANDFPTVEEGPGSLTFTLVQSKLRRLIERTSFAMAQQDVRYYLNGMLL
EVSAGILRAVATDGHRLAMCSMSADIEHADRHQVIVPRKGILEMARLLTEQDGTVSIVLGQHHIRATTGEFTFTSKLVDG
KFPDYERVLPKGGDKLVLGDRQALREAFSRTAILSNEKYRGIRLQLASGQLKIQANNPEQEEAEEEISVEYNGDSLEIGF
NVSYLLDVLGVMTTEQVRLILSDSNSSALVQESDNDDSAYVVMPMRL
>Mature_367_residues
MHFTIQREALLKPLQLVAGVVERRQTLPVLSNVLLVVEGQQLSLTGTDLEVELVGRVQLEEPAEPGEITVPARKLMDICK
SLSNDALIDIKLDDSKLIVKAGRSRFTLSTLPANDFPTVEEGPGSLTFTLVQSKLRRLIERTSFAMAQQDVRYYLNGMLL
EVSAGILRAVATDGHRLAMCSMSADIEHADRHQVIVPRKGILEMARLLTEQDGTVSIVLGQHHIRATTGEFTFTSKLVDG
KFPDYERVLPKGGDKLVLGDRQALREAFSRTAILSNEKYRGIRLQLASGQLKIQANNPEQEEAEEEISVEYNGDSLEIGF
NVSYLLDVLGVMTTEQVRLILSDSNSSALVQESDNDDSAYVVMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=55.858310626703, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40631; Mature: 40631

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFTIQREALLKPLQLVAGVVERRQTLPVLSNVLLVVEGQQLSLTGTDLEVELVGRVQLE
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCEEEEEEEEEEEC
EPAEPGEITVPARKLMDICKSLSNDALIDIKLDDSKLIVKAGRSRFTLSTLPANDFPTVE
CCCCCCEEEECHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCC
EGPGSLTFTLVQSKLRRLIERTSFAMAQQDVRYYLNGMLLEVSAGILRAVATDGHRLAMC
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCEEEEE
SMSADIEHADRHQVIVPRKGILEMARLLTEQDGTVSIVLGQHHIRATTGEFTFTSKLVDG
ECCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCEEEEEEECCC
KFPDYERVLPKGGDKLVLGDRQALREAFSRTAILSNEKYRGIRLQLASGQLKIQANNPEQ
CCCCHHHHCCCCCCEEEECCHHHHHHHHHHHEEECCCCCCEEEEEEECCEEEEEECCCCH
EEAEEEISVEYNGDSLEIGFNVSYLLDVLGVMTTEQVRLILSDSNSSALVQESDNDDSAY
HHHHHHEEEEECCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCEE
VVMPMRL
EEEEECC
>Mature Secondary Structure
MHFTIQREALLKPLQLVAGVVERRQTLPVLSNVLLVVEGQQLSLTGTDLEVELVGRVQLE
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCEEEEEEEEEEEC
EPAEPGEITVPARKLMDICKSLSNDALIDIKLDDSKLIVKAGRSRFTLSTLPANDFPTVE
CCCCCCEEEECHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCC
EGPGSLTFTLVQSKLRRLIERTSFAMAQQDVRYYLNGMLLEVSAGILRAVATDGHRLAMC
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCEEEEE
SMSADIEHADRHQVIVPRKGILEMARLLTEQDGTVSIVLGQHHIRATTGEFTFTSKLVDG
ECCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCEEEEEEECCC
KFPDYERVLPKGGDKLVLGDRQALREAFSRTAILSNEKYRGIRLQLASGQLKIQANNPEQ
CCCCHHHHCCCCCCEEEECCHHHHHHHHHHHEEECCCCCCEEEEEEECCEEEEEECCCCH
EEAEEEISVEYNGDSLEIGFNVSYLLDVLGVMTTEQVRLILSDSNSSALVQESDNDDSAY
HHHHHHEEEEECCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCEE
VVMPMRL
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]