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The map label for this gene is proP [H]

Identifier: 62391912

GI number: 62391912

Start: 3245868

End: 3247382

Strand: Reverse

Name: proP [H]

Synonym: cg3395

Alternate gene names: 62391912

Gene position: 3247382-3245868 (Counterclockwise)

Preceding gene: 62391913

Following gene: 62391904

Centisome position: 98.92

GC content: 53.86

Gene sequence:

>1515_bases
GTGAGCCCGATTCGCTCAAAAAAGAAAATCAAGAACGAACCAAGACTAACAGTCGATGACGTCAACGTTGTTCCCCCAAA
GAAGATCCGTCCGGCCATTAAAGGCACTGTGGTGGGTAACTTCATGGAGTGGTACGACTTCGGAATTTATGGTTATTTGA
CGGTCACGATGACCGCAGTATTTACCCAAGGCCTGCCGCAAGAATGGCAGTTGTTGGCCGTGATGTTTGGTTTCGCGGTG
TCTTATTTAGTTCGCCCGCTTGGTGGCCTGGTTCTGGGTCCGCTTGGCGATAAGGTTGGCCGCCAGAAGGTTTTGTACGT
AACCATGGCCATGATGGCCGTGTCTACGGCGCTGATTGGCTTGCTGCCGACGGCGGCGTCGATAGGCGCTTGGGCTTTGG
TGCTTTTGTACCTGTTGAAGATGGTGCAGGGATTTTCGACTGGTGGTGAATATGCTGGTGCGACCACCTATGTTGCTGAG
TTTGCGCCGGATCGTCGACGAGGTTTCTTCGGAGCTTTCCTAGATATGGGTTCCTACCTGGGCTTTGCCGCGGGTGCTTC
GGTCGTGGCGATTACTACCTGGGTGACCACTCACTTCTATGGCGCAACTGCCATGGAGGATTTCGGTTGGCGTATTCCTT
TCCTGACTGCGATCCCGCTGGGCATCATTGCGGTGTACCTGCGTACTCGTATCCCTGAGACCCCTGCGTTTGAGAACAAC
CAAGACGAGCCAAATGCAGTTGTTGAAAAGGACACTGAAGATCCTTATGCACGCCTGGGCCTGGCTGGTGTTATCCGCCA
CCACTGGCGTCCACTGCTGATTGGTATTGCGATTGTGGCAGCGACCAACACCGCCGGTTACGCGCTAACCAGTTACATGC
CTGTGTATCTAGAGGAGCAGATCGGTCTGCACTCCGCATCCGCTGCCGCTGTGACCGTGCCGATTCTGGTTGTTATGTCC
CTGCTGCTGCCATTTGTTGGTATGTGGAGTGACCGCGTGGGCCGCAAGCCTGTCTACGCGACTGCTGTTGCGGCAACGCT
GATCTTGATGGTTCCTGCCTTCTTGATCATGAACACCGGAACCATCGGCGCCGTACTGATTGCACTGTCCATGGTTGCTA
TTCCAACCGGTTTGTATGTGGCACTGTCCGCATCTGCCCTGCCAGCGCTGTTCCCAACCGCGTCACGATTCTCTGGAATG
GGTATTTCCTACAACATTTCCGTGTCGTTGTTCGGTGGTACAACCCCGCTGATCACCCAGTTCCTGCTGCAGAAGACTGG
CCTGGATATCGTTCCAGCGCTCTACATCATGTTCTTCTCTGCAATCGCAGGTGTGGCGCTGCTGTTCATGACCGAGTCTT
CACAGAAGCCACTGCTTGGCTCATTCCCAACCGTGGAAACCAAGTCTGAGGCCGTGGAGATCGTGAAGAACCAGGACGAG
GATCCAAATATTGATCTTTCCCATATGCCGTTTCCTGATGAGGAAAACGTAGGTGCTGAAAAGCAAAACGCATAA

Upstream 100 bases:

>100_bases
CAGGAAGAAGTTAACACCGCCCAGGGGTGCGTTGGATGATGATCATCTACAAACAAACATTCCGTTATGCACTCATAAGA
TATGACGAGAGGTTTTACTC

Downstream 100 bases:

>100_bases
CCGTAAAGCCCGCTGCAAGGCGGGCTTTTTTGGTGGCCGGGGGAGTCGAAAAGTGCTTATCGACGAGTGCTGCGTCGGTT
TATGAGATGCTTTTGTAGTC

Product: proline/ectoine carrier

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSPIRSKKKIKNEPRLTVDDVNVVPPKKIRPAIKGTVVGNFMEWYDFGIYGYLTVTMTAVFTQGLPQEWQLLAVMFGFAV
SYLVRPLGGLVLGPLGDKVGRQKVLYVTMAMMAVSTALIGLLPTAASIGAWALVLLYLLKMVQGFSTGGEYAGATTYVAE
FAPDRRRGFFGAFLDMGSYLGFAAGASVVAITTWVTTHFYGATAMEDFGWRIPFLTAIPLGIIAVYLRTRIPETPAFENN
QDEPNAVVEKDTEDPYARLGLAGVIRHHWRPLLIGIAIVAATNTAGYALTSYMPVYLEEQIGLHSASAAAVTVPILVVMS
LLLPFVGMWSDRVGRKPVYATAVAATLILMVPAFLIMNTGTIGAVLIALSMVAIPTGLYVALSASALPALFPTASRFSGM
GISYNISVSLFGGTTPLITQFLLQKTGLDIVPALYIMFFSAIAGVALLFMTESSQKPLLGSFPTVETKSEAVEIVKNQDE
DPNIDLSHMPFPDEENVGAEKQNA

Sequences:

>Translated_504_residues
MSPIRSKKKIKNEPRLTVDDVNVVPPKKIRPAIKGTVVGNFMEWYDFGIYGYLTVTMTAVFTQGLPQEWQLLAVMFGFAV
SYLVRPLGGLVLGPLGDKVGRQKVLYVTMAMMAVSTALIGLLPTAASIGAWALVLLYLLKMVQGFSTGGEYAGATTYVAE
FAPDRRRGFFGAFLDMGSYLGFAAGASVVAITTWVTTHFYGATAMEDFGWRIPFLTAIPLGIIAVYLRTRIPETPAFENN
QDEPNAVVEKDTEDPYARLGLAGVIRHHWRPLLIGIAIVAATNTAGYALTSYMPVYLEEQIGLHSASAAAVTVPILVVMS
LLLPFVGMWSDRVGRKPVYATAVAATLILMVPAFLIMNTGTIGAVLIALSMVAIPTGLYVALSASALPALFPTASRFSGM
GISYNISVSLFGGTTPLITQFLLQKTGLDIVPALYIMFFSAIAGVALLFMTESSQKPLLGSFPTVETKSEAVEIVKNQDE
DPNIDLSHMPFPDEENVGAEKQNA
>Mature_503_residues
SPIRSKKKIKNEPRLTVDDVNVVPPKKIRPAIKGTVVGNFMEWYDFGIYGYLTVTMTAVFTQGLPQEWQLLAVMFGFAVS
YLVRPLGGLVLGPLGDKVGRQKVLYVTMAMMAVSTALIGLLPTAASIGAWALVLLYLLKMVQGFSTGGEYAGATTYVAEF
APDRRRGFFGAFLDMGSYLGFAAGASVVAITTWVTTHFYGATAMEDFGWRIPFLTAIPLGIIAVYLRTRIPETPAFENNQ
DEPNAVVEKDTEDPYARLGLAGVIRHHWRPLLIGIAIVAATNTAGYALTSYMPVYLEEQIGLHSASAAAVTVPILVVMSL
LLPFVGMWSDRVGRKPVYATAVAATLILMVPAFLIMNTGTIGAVLIALSMVAIPTGLYVALSASALPALFPTASRFSGMG
ISYNISVSLFGGTTPLITQFLLQKTGLDIVPALYIMFFSAIAGVALLFMTESSQKPLLGSFPTVETKSEAVEIVKNQDED
PNIDLSHMPFPDEENVGAEKQNA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=486, Percent_Identity=38.8888888888889, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1788942, Length=443, Percent_Identity=28.6681715575621, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788292, Length=445, Percent_Identity=28.7640449438202, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1789941, Length=452, Percent_Identity=28.7610619469027, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87082404, Length=368, Percent_Identity=23.0978260869565, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082231, Length=410, Percent_Identity=23.1707317073171, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 54276; Mature: 54145

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SPIRSKKKIKNEPRLTVDDVNVVPPKKIRPAIKGTVVGNFMEWYDFGIYGYLTVTMTAV
CCCCCHHHHCCCCCEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
FTQGLPQEWQLLAVMFGFAVSYLVRPLGGLVLGPLGDKVGRQKVLYVTMAMMAVSTALIG
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHCCHHHHHHHHHHHHHHHHHHH
LLPTAASIGAWALVLLYLLKMVQGFSTGGEYAGATTYVAEFAPDRRRGFFGAFLDMGSYL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHH
GFAAGASVVAITTWVTTHFYGATAMEDFGWRIPFLTAIPLGIIAVYLRTRIPETPAFENN
HHHCCCCCEEHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
QDEPNAVVEKDTEDPYARLGLAGVIRHHWRPLLIGIAIVAATNTAGYALTSYMPVYLEEQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHH
IGLHSASAAAVTVPILVVMSLLLPFVGMWSDRVGRKPVYATAVAATLILMVPAFLIMNTG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TIGAVLIALSMVAIPTGLYVALSASALPALFPTASRFSGMGISYNISVSLFGGTTPLITQ
HHHHHHHHHHHHHHCCCHHEEEEHHHHHHHCCCHHHHCCCCEEEEEEEEEECCCHHHHHH
FLLQKTGLDIVPALYIMFFSAIAGVALLFMTESSQKPLLGSFPTVETKSEAVEIVKNQDE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHCCCC
DPNIDLSHMPFPDEENVGAEKQNA
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]