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The map label for this gene is ogt [H]

Identifier: 62391852

GI number: 62391852

Start: 3178035

End: 3178511

Strand: Reverse

Name: ogt [H]

Synonym: cg3331

Alternate gene names: 62391852

Gene position: 3178511-3178035 (Counterclockwise)

Preceding gene: 62391853

Following gene: 62391848

Centisome position: 96.83

GC content: 52.41

Gene sequence:

>477_bases
ATGCACCATCTACGCTATGAATCACCAATCGGAGAGCTTCTTCTTGTTGCAAGTGACCAAGGGCTAACCTATGTGGCATT
CTCCGATGAAAACTACGCAGCTTGTACTGTCGGGTCGACCCCGGGAACCAATGCGGTGCTGGAACAGGCAGTTGCTGAGC
TTGAAGAATACTTCGCAGGGAAACGTAAAGAGTTCAGCACTCCCCTGGATTGGCCAAGCCAAAATCTGCTGAGCTTCCGC
GGTAAAGTGCAGGAATTTTTGCTGTCCATTCCTTATGGGGAGAGTAAAACTTACAAACAGATCGCCGCTGAGCTTAATAA
TGTGGGCGCGGTTCGTGCAGTGGGAAGCGCCTGCGCCACCAACCCCTTGCCAATCTTTGCTCCTTGTCACCGAGTACTGC
GCACTGATGGGGCGTTAGGTGGCTACAGAGGAGGCTTGGAAGCAAAACAGTGGCTGTTGGAGCTGGAACGTCCTTAG

Upstream 100 bases:

>100_bases
ATGGGCTCAACGCTGCAAACATCATGGAGGGGCACCGGCTCGTTGAGCAGGGTAAAACCTCAGGAAAAATTGTTGTGAGG
GTATAAAGAGGACTTGAAAA

Downstream 100 bases:

>100_bases
TTTGTGTCCGCGCACGGAGCGGTAATCCAAAATTTAATGAAGCCGCCAATCTCGTGGAGTACTGCATTGTGAAGCCCGGT
ACGTCCTAGGCTGTTGACGT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MHHLRYESPIGELLLVASDQGLTYVAFSDENYAACTVGSTPGTNAVLEQAVAELEEYFAGKRKEFSTPLDWPSQNLLSFR
GKVQEFLLSIPYGESKTYKQIAAELNNVGAVRAVGSACATNPLPIFAPCHRVLRTDGALGGYRGGLEAKQWLLELERP

Sequences:

>Translated_158_residues
MHHLRYESPIGELLLVASDQGLTYVAFSDENYAACTVGSTPGTNAVLEQAVAELEEYFAGKRKEFSTPLDWPSQNLLSFR
GKVQEFLLSIPYGESKTYKQIAAELNNVGAVRAVGSACATNPLPIFAPCHRVLRTDGALGGYRGGLEAKQWLLELERP
>Mature_158_residues
MHHLRYESPIGELLLVASDQGLTYVAFSDENYAACTVGSTPGTNAVLEQAVAELEEYFAGKRKEFSTPLDWPSQNLLSFR
GKVQEFLLSIPYGESKTYKQIAAELNNVGAVRAVGSACATNPLPIFAPCHRVLRTDGALGGYRGGLEAKQWLLELERP

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=166, Percent_Identity=37.3493975903614, Blast_Score=90, Evalue=7e-19,
Organism=Escherichia coli, GI1788542, Length=157, Percent_Identity=31.8471337579618, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1787596, Length=104, Percent_Identity=40.3846153846154, Blast_Score=78, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=79, Percent_Identity=46.8354430379747, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115533068, Length=79, Percent_Identity=46.8354430379747, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17276; Mature: 17276

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHLRYESPIGELLLVASDQGLTYVAFSDENYAACTVGSTPGTNAVLEQAVAELEEYFAG
CCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCC
KRKEFSTPLDWPSQNLLSFRGKVQEFLLSIPYGESKTYKQIAAELNNVGAVRAVGSACAT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCC
NPLPIFAPCHRVLRTDGALGGYRGGLEAKQWLLELERP
CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MHHLRYESPIGELLLVASDQGLTYVAFSDENYAACTVGSTPGTNAVLEQAVAELEEYFAG
CCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCC
KRKEFSTPLDWPSQNLLSFRGKVQEFLLSIPYGESKTYKQIAAELNNVGAVRAVGSACAT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCC
NPLPIFAPCHRVLRTDGALGGYRGGLEAKQWLLELERP
CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]