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The map label for this gene is trmD

Identifier: 62390887

GI number: 62390887

Start: 2132243

End: 2133112

Strand: Reverse

Name: trmD

Synonym: cg2249

Alternate gene names: 62390887

Gene position: 2133112-2132243 (Counterclockwise)

Preceding gene: 62390889

Following gene: 62390886

Centisome position: 64.98

GC content: 61.03

Gene sequence:

>870_bases
GTGACTAGCTCCGAAAATCTTGATTCCCACCGTCTGCGGCTTGATGTCGTGACCATTTTCCCCGAATACCTGGATCCTCT
GCGCCATGCCCTGCTGGGTAAGGCGATTGAGGATGGCATTTTGGAAGTCGGTGTTCATGATCTTCGGAATTGGGCGACCG
GCGGACACAAGGCCGTCGACGATACCCCGTATGGCGGTGGCCCGGGCATGGTGATGAAGCCAGAGGTCTGGGGGCCAGCG
CTTGATGATGTCGCCGCAGGCCGGGTGAGCGGTGCGGAACTCGATTCGGCCTCGCTGCACCTGAAAAATGTGCGCCATGA
TGAGCTGGGTGGCGTCGAAAAGCGTGCTTATGTCGTGGAAGAAGACCGCGACCTGCCGCTGTTGCTGGTGCCCACCCCGG
CTGGCAAGCCGTTCACGCAGGCGGATGCGCAGGCGTGGTCCAACGAGGAGCACATTGTGTTCGCGTGCGGGCGCTACGAG
GGCATTGACCAGCGCGTTATTGATGATGCCGCCAACCGCTACCGCGTGCGCGAGGTATCGATCGGCGATTATGTGCTGAT
CGGCGGGGAAGTGGCAGTCCTGGTCATCGCGGAAGCCGTCGTGCGCCTGATCCCTGGCGTGCTCGGAAACCGTCGTAGCC
ACGAAGAAGACAGCTTCTCCGATGGCCTGCTCGAAGGCCCGTCGTACACAAAGCCGCGCACCTGGCGCGGACTTGACGTC
CCCGAAGTACTATTCTCGGGCAACCACGCCAAGGTCGATCGCTGGCGACGCGATCAGGCGCTCCTACGCACCCAGGCAAT
TAGGCCTGAGCTTATCGACGCATCCCTCCTCGATTCCACCGACCTCAAAGTATTGGGACTGGACAAATGA

Upstream 100 bases:

>100_bases
AGCACCAAGAGCCCACCCACGCTGCCGATTGCTAAAGCTTGTCGCAAAAGTGTGTGAGGCTTCATGCCTCCAGCGTAGAC
CTCTTGGTAGATTGAAGTCC

Downstream 100 bases:

>100_bases
CAGAGACCACACCTCAACCCCCAAAGCCAGAGCCCGAGCTCCCGAAATTTCTCAGCAACCCAGAACGCGCCGACATCATT
TTGTTCATCGCACTCATTGT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTSSENLDSHRLRLDVVTIFPEYLDPLRHALLGKAIEDGILEVGVHDLRNWATGGHKAVDDTPYGGGPGMVMKPEVWGPA
LDDVAAGRVSGAELDSASLHLKNVRHDELGGVEKRAYVVEEDRDLPLLLVPTPAGKPFTQADAQAWSNEEHIVFACGRYE
GIDQRVIDDAANRYRVREVSIGDYVLIGGEVAVLVIAEAVVRLIPGVLGNRRSHEEDSFSDGLLEGPSYTKPRTWRGLDV
PEVLFSGNHAKVDRWRRDQALLRTQAIRPELIDASLLDSTDLKVLGLDK

Sequences:

>Translated_289_residues
MTSSENLDSHRLRLDVVTIFPEYLDPLRHALLGKAIEDGILEVGVHDLRNWATGGHKAVDDTPYGGGPGMVMKPEVWGPA
LDDVAAGRVSGAELDSASLHLKNVRHDELGGVEKRAYVVEEDRDLPLLLVPTPAGKPFTQADAQAWSNEEHIVFACGRYE
GIDQRVIDDAANRYRVREVSIGDYVLIGGEVAVLVIAEAVVRLIPGVLGNRRSHEEDSFSDGLLEGPSYTKPRTWRGLDV
PEVLFSGNHAKVDRWRRDQALLRTQAIRPELIDASLLDSTDLKVLGLDK
>Mature_288_residues
TSSENLDSHRLRLDVVTIFPEYLDPLRHALLGKAIEDGILEVGVHDLRNWATGGHKAVDDTPYGGGPGMVMKPEVWGPAL
DDVAAGRVSGAELDSASLHLKNVRHDELGGVEKRAYVVEEDRDLPLLLVPTPAGKPFTQADAQAWSNEEHIVFACGRYEG
IDQRVIDDAANRYRVREVSIGDYVLIGGEVAVLVIAEAVVRLIPGVLGNRRSHEEDSFSDGLLEGPSYTKPRTWRGLDVP
EVLFSGNHAKVDRWRRDQALLRTQAIRPELIDASLLDSTDLKVLGLDK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=274, Percent_Identity=34.3065693430657, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_CORGL (Q8NNX7)

Other databases:

- EMBL:   BA000036
- EMBL:   BX927154
- RefSeq:   NP_601253.1
- RefSeq:   YP_226289.1
- ProteinModelPortal:   Q8NNX7
- SMR:   Q8NNX7
- GeneID:   1020004
- GeneID:   3343898
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg2249
- KEGG:   cgl:NCgl1972
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   CGLU196627:CG2249-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 31791; Mature: 31660

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSENLDSHRLRLDVVTIFPEYLDPLRHALLGKAIEDGILEVGVHDLRNWATGGHKAVD
CCCCCCCCCCEEEEEHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DTPYGGGPGMVMKPEVWGPALDDVAAGRVSGAELDSASLHLKNVRHDELGGVEKRAYVVE
CCCCCCCCCCEECCCCCCCCHHHHHHCCCCCCCCCCCCEECCCCCHHHHCCCCCEEEEEE
EDRDLPLLLVPTPAGKPFTQADAQAWSNEEHIVFACGRYEGIDQRVIDDAANRYRVREVS
CCCCCCEEEEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHEEEEEEC
IGDYVLIGGEVAVLVIAEAVVRLIPGVLGNRRSHEEDSFSDGLLEGPSYTKPRTWRGLDV
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
PEVLFSGNHAKVDRWRRDQALLRTQAIRPELIDASLLDSTDLKVLGLDK
HHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure 
TSSENLDSHRLRLDVVTIFPEYLDPLRHALLGKAIEDGILEVGVHDLRNWATGGHKAVD
CCCCCCCCCEEEEEHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DTPYGGGPGMVMKPEVWGPALDDVAAGRVSGAELDSASLHLKNVRHDELGGVEKRAYVVE
CCCCCCCCCCEECCCCCCCCHHHHHHCCCCCCCCCCCCEECCCCCHHHHCCCCCEEEEEE
EDRDLPLLLVPTPAGKPFTQADAQAWSNEEHIVFACGRYEGIDQRVIDDAANRYRVREVS
CCCCCCEEEEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHEEEEEEC
IGDYVLIGGEVAVLVIAEAVVRLIPGVLGNRRSHEEDSFSDGLLEGPSYTKPRTWRGLDV
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
PEVLFSGNHAKVDRWRRDQALLRTQAIRPELIDASLLDSTDLKVLGLDK
HHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626