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The map label for this gene is amt

Identifier: 62390465

GI number: 62390465

Start: 1676732

End: 1678090

Strand: Reverse

Name: amt

Synonym: cg1785

Alternate gene names: 62390465

Gene position: 1678090-1676732 (Counterclockwise)

Preceding gene: 62390466

Following gene: 62390464

Centisome position: 51.12

GC content: 55.41

Gene sequence:

>1359_bases
ATGGACCCCTCAGATCTAGCCTGGATTCTCGCAGCTTTTGCGTTGGTAAGCCTGATGTTCCCCGGATTGTCCCTGCTCTA
CGGCGGCATGCTGGGTGGGCAACACGTTCTTAACACGTTCATGATGGTTATGAGCTCACTTGGAATCATCAGCCTTGTGT
ACATCATTTATGGACACGGACTTGTCTTAGGAAACTCCATCGGTGGGTGGGGAATTATCGGAAATCCCCTTGAATACTTC
GGCTTCCGCAACATTATGGAAGATGACGGCACCGGAGACCTCATGTGGGCCGGCTTCTACATTCTGTTCGCTGCAATCTC
ACTCGCACTTGTTTCATCTGGTGCAGCGGGGCGCATGCGCTTTGGAGCGTGGCTGGTCTTCGGTGTCCTGTGGTTCACCT
TTGTGTACGCGCCACTGGCACACTGGGTTTTCGCTATCGATGATCCTGAGTCCGGCTACGTGGGTGGCTGGATGAAAAAT
GTGCTTGAGTTCCACGACTTTGCTGGTGGAACGGCAGTGCACATGAATGCGGGTGCGTCTGGACTCGCGCTGGCAATAGT
GCTGGGACGCCGCCACTCCATGGCTGTGCGTCCACACAACCTTCCACTGATTTTGATTGGTGCAGGACTGATCGTTGCGG
GCTGGTTCGGATTCAATGGTGGTACCGCAGGTGGTGCCAACTTCCTCGCAAGCTACGTGGTCGTTACCTCTCTCATTGCT
GCAGCTGGCGGAATGATGGGCTTCATGCTCGTTGAACGTGTGTTCAGCGGAAAACCCACTTTCTTTGGCTCGGCAACCGG
CACAATCGCAGGCCTTGTGGCTATCACCCCGGCCGCGGATGCAGTGAGCCCGCTCGGAGCATTCGCCGTCGGAGCGCTCG
GCGCAGTTGTCTCCTTCTGGGCAATTAGCTGGAAGAAGGGACACCGAGTCGATGATTCCTTCGATGTGTTCGCAGTCCAC
GGAATGGCCGGCATTGCAGGTGCACTGTTTGTCATGCTCTTTGGCGATCCACTAGCACCAGCGGGAGTTTCCGGAGTCTT
CTTCGGTGGCGAACTCTCCCTGCTGTGGAGGGAACCACTGGCCATCATCGTGACCCTTACATACGCATTCGGCGTGACCT
GGTTGATTGCCACGATCTTGAACAAGTTCATGACTCTGCGCATCACCTCCGAAGCCGAATATGAAGGCATTGACCGCGCA
GAACACGCAGAATCTGCCTACCACCTCAATTCCAACGGAATTGGGATGGCAACCCGCACCAATTTCGGACCTGAAATCCC
CGAGGAAACCGTGCCCGACGCCGTGCAGGTGGGCGTCGATAAGCAAAAAATCGCTGATACTCGAAAGGCCTCAAAATGA

Upstream 100 bases:

>100_bases
GAAATCTATAGAACGATAGGTAAAAACTGGACTAGGTTTATCTATAGCGGAATAGAAAATACTCCGCTCGACAGCATCAC
TTAGCTGAAAGGCCTTTAAC

Downstream 100 bases:

>100_bases
CCGCAACCTACACCACTGAAACCGCCATCAATTTCTTGTTCTTGAGCGAACCGGACATGATCGCGGCCGGAGTCAAAGAC
GTCGCGCAATGCGTCGATGT

Product: high-affinity ammonia permease

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MDPSDLAWILAAFALVSLMFPGLSLLYGGMLGGQHVLNTFMMVMSSLGIISLVYIIYGHGLVLGNSIGGWGIIGNPLEYF
GFRNIMEDDGTGDLMWAGFYILFAAISLALVSSGAAGRMRFGAWLVFGVLWFTFVYAPLAHWVFAIDDPESGYVGGWMKN
VLEFHDFAGGTAVHMNAGASGLALAIVLGRRHSMAVRPHNLPLILIGAGLIVAGWFGFNGGTAGGANFLASYVVVTSLIA
AAGGMMGFMLVERVFSGKPTFFGSATGTIAGLVAITPAADAVSPLGAFAVGALGAVVSFWAISWKKGHRVDDSFDVFAVH
GMAGIAGALFVMLFGDPLAPAGVSGVFFGGELSLLWREPLAIIVTLTYAFGVTWLIATILNKFMTLRITSEAEYEGIDRA
EHAESAYHLNSNGIGMATRTNFGPEIPEETVPDAVQVGVDKQKIADTRKASK

Sequences:

>Translated_452_residues
MDPSDLAWILAAFALVSLMFPGLSLLYGGMLGGQHVLNTFMMVMSSLGIISLVYIIYGHGLVLGNSIGGWGIIGNPLEYF
GFRNIMEDDGTGDLMWAGFYILFAAISLALVSSGAAGRMRFGAWLVFGVLWFTFVYAPLAHWVFAIDDPESGYVGGWMKN
VLEFHDFAGGTAVHMNAGASGLALAIVLGRRHSMAVRPHNLPLILIGAGLIVAGWFGFNGGTAGGANFLASYVVVTSLIA
AAGGMMGFMLVERVFSGKPTFFGSATGTIAGLVAITPAADAVSPLGAFAVGALGAVVSFWAISWKKGHRVDDSFDVFAVH
GMAGIAGALFVMLFGDPLAPAGVSGVFFGGELSLLWREPLAIIVTLTYAFGVTWLIATILNKFMTLRITSEAEYEGIDRA
EHAESAYHLNSNGIGMATRTNFGPEIPEETVPDAVQVGVDKQKIADTRKASK
>Mature_452_residues
MDPSDLAWILAAFALVSLMFPGLSLLYGGMLGGQHVLNTFMMVMSSLGIISLVYIIYGHGLVLGNSIGGWGIIGNPLEYF
GFRNIMEDDGTGDLMWAGFYILFAAISLALVSSGAAGRMRFGAWLVFGVLWFTFVYAPLAHWVFAIDDPESGYVGGWMKN
VLEFHDFAGGTAVHMNAGASGLALAIVLGRRHSMAVRPHNLPLILIGAGLIVAGWFGFNGGTAGGANFLASYVVVTSLIA
AAGGMMGFMLVERVFSGKPTFFGSATGTIAGLVAITPAADAVSPLGAFAVGALGAVVSFWAISWKKGHRVDDSFDVFAVH
GMAGIAGALFVMLFGDPLAPAGVSGVFFGGELSLLWREPLAIIVTLTYAFGVTWLIATILNKFMTLRITSEAEYEGIDRA
EHAESAYHLNSNGIGMATRTNFGPEIPEETVPDAVQVGVDKQKIADTRKASK

Specific function: Involved in the uptake of ammonia and methylammonia. Utilizes uniport of ammonium and uses the membrane potential as driving force

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family

Homologues:

Organism=Escherichia coli, GI1786656, Length=420, Percent_Identity=34.2857142857143, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17550182, Length=346, Percent_Identity=25.1445086705202, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI133931100, Length=454, Percent_Identity=25.7709251101322, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17550180, Length=442, Percent_Identity=23.5294117647059, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI32564042, Length=429, Percent_Identity=26.1072261072261, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324187, Length=476, Percent_Identity=29.4117647058824, Blast_Score=193, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6325396, Length=477, Percent_Identity=28.5115303983228, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321559, Length=423, Percent_Identity=28.8416075650118, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI161078314, Length=422, Percent_Identity=27.9620853080569, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMT_CORGL (P54146)

Other databases:

- EMBL:   X93513
- EMBL:   BA000036
- EMBL:   BX927152
- RefSeq:   NP_600797.1
- RefSeq:   YP_225867.1
- ProteinModelPortal:   P54146
- GeneID:   1019551
- GeneID:   3343475
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg1785
- KEGG:   cgl:NCgl1521
- HOGENOM:   HBG677626
- OMA:   DLAWILA
- ProtClustDB:   CLSK633478
- BioCyc:   CGLU196627:CG1785-MONOMER
- InterPro:   IPR001905
- InterPro:   IPR018047
- PANTHER:   PTHR11730
- TIGRFAMs:   TIGR00836

Pfam domain/function: PF00909 Ammonium_transp; SSF111352 RH_like_transpt

EC number: NA

Molecular weight: Translated: 47700; Mature: 47700

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x140ad094)-; HASH(0x134287f8)-; HASH(0x133384a4)-; HASH(0x13f68724)-; HASH(0x13f65234)-; HASH(0x139ec7ec)-; HASH(0x13cf272c)-; HASH(0x13f2ea14)-; HASH(0x13d4c5f0)-; HASH(0x13cb2164)-; HASH(0x13fef894)-; HASH(0x1045897c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPSDLAWILAAFALVSLMFPGLSLLYGGMLGGQHVLNTFMMVMSSLGIISLVYIIYGHG
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LVLGNSIGGWGIIGNPLEYFGFRNIMEDDGTGDLMWAGFYILFAAISLALVSSGAAGRMR
EEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
FGAWLVFGVLWFTFVYAPLAHWVFAIDDPESGYVGGWMKNVLEFHDFAGGTAVHMNAGAS
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCC
GLALAIVLGRRHSMAVRPHNLPLILIGAGLIVAGWFGFNGGTAGGANFLASYVVVTSLIA
HHHHHHHHCCCCCEEECCCCCCEEEEECHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHH
AAGGMMGFMLVERVFSGKPTFFGSATGTIAGLVAITPAADAVSPLGAFAVGALGAVVSFW
HHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHH
AISWKKGHRVDDSFDVFAVHGMAGIAGALFVMLFGDPLAPAGVSGVFFGGELSLLWREPL
HHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEHHCCH
AIIVTLTYAFGVTWLIATILNKFMTLRITSEAEYEGIDRAEHAESAYHLNSNGIGMATRT
HHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHCHHEEECCCCCCEEEEC
NFGPEIPEETVPDAVQVGVDKQKIADTRKASK
CCCCCCCHHHCCCHHHHCCCHHHHHHHHCCCH
>Mature Secondary Structure
MDPSDLAWILAAFALVSLMFPGLSLLYGGMLGGQHVLNTFMMVMSSLGIISLVYIIYGHG
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LVLGNSIGGWGIIGNPLEYFGFRNIMEDDGTGDLMWAGFYILFAAISLALVSSGAAGRMR
EEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
FGAWLVFGVLWFTFVYAPLAHWVFAIDDPESGYVGGWMKNVLEFHDFAGGTAVHMNAGAS
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCC
GLALAIVLGRRHSMAVRPHNLPLILIGAGLIVAGWFGFNGGTAGGANFLASYVVVTSLIA
HHHHHHHHCCCCCEEECCCCCCEEEEECHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHH
AAGGMMGFMLVERVFSGKPTFFGSATGTIAGLVAITPAADAVSPLGAFAVGALGAVVSFW
HHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHH
AISWKKGHRVDDSFDVFAVHGMAGIAGALFVMLFGDPLAPAGVSGVFFGGELSLLWREPL
HHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEHHCCH
AIIVTLTYAFGVTWLIATILNKFMTLRITSEAEYEGIDRAEHAESAYHLNSNGIGMATRT
HHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHCHHEEECCCCCCEEEEC
NFGPEIPEETVPDAVQVGVDKQKIADTRKASK
CCCCCCCHHHCCCHHHHCCCHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8621394; 12948626; 2666264