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The map label for this gene is dapE

Identifier: 62389999

GI number: 62389999

Start: 1157199

End: 1158308

Strand: Direct

Name: dapE

Synonym: cg1260

Alternate gene names: 62389999

Gene position: 1157199-1158308 (Clockwise)

Preceding gene: 62389995

Following gene: 62390000

Centisome position: 35.25

GC content: 54.14

Gene sequence:

>1110_bases
GTGAACTCTGAACTCAAACCAGGATTAGATCTCCTCGGCGACCCAATTGTCCTTACTCAACGTTTGGTAGATATACCGAG
TCCGTCGGGTCAGGAAAAGCAGATTGCTGATGAAATTGAAGATGCCCTTCGGAACCTTAATCTACCTGGTGTAGAGGTCT
TCCGCTTCAACAACAACGTTCTTGCTCGCACGAACAGGGGATTGGCCTCGAGGGTCATGCTTGCTGGTCATATCGATACA
GTGCCGATCGCGGACAATCTGCCAAGCCGTGTGGAAGACGGCATCATGTATGGCTGTGGCACCGTCGATATGAAATCTGG
GTTGGCGGTGTATTTGCATACTTTTGCCACCTTGGCCACGTCGACTGAGCTTAAACATGATCTGACGCTGATTGCGTATG
AGTGCGAGGAAGTTGCTGATCACCTCAATGGTTTGGGCCACATTCGCGATGAGCATCCGGAGTGGTTGGCGGCTGATTTG
GCGTTGTTGGGTGAGCCTACTGGCGGCTGGATTGAGGCGGGCTGCCAGGGCAATCTGCGCATCAAGGTGACGGCGCATGG
TGTGCGTGCCCATTCGGCGAGAAGCTGGTTGGGTGATAATGCGATGCATAAGTTGTCGCCGATCATTTCGAAGGTTGCTG
CGTATAAGGCCGCAGAAGTCAACATTGATGGCTTGACCTACCGTGAAGGCCTCAACATCGTTTTCTGCGAATCGGGCGTG
GCAAACAACGTCATTCCAGACCTCGCGTGGATGAACCTCAACTTCCGTTTCGCGCCGAATCGCGATCTCAACGAGGCGAT
CGAGCATGTCGTCGAAACGCTTGAGCTTGACGGTCAAGACGGCATCGAATGGGCCGTAGAAGACGGGGCAGGCGGTGCCC
TTCCAGGCTTGGGGCAGCAGGTGACAAGCGGGCTTATCGACGCCGTCGGCCGCGAAAAAATCCGCGCAAAATTCGGCTGG
ACCGATGTCTCACGTTTTTCAGCCATGGGAATTCCAGCCCTAAACTTTGGCGCTGGTGATCCAAGTTTCGCGCATAAACG
CGACGAGCAGTGCCCAGTGGAGCAAATCACGGATGTGGCAGCAATTTTGAAGCAGTACCTGAGCGAGTAA

Upstream 100 bases:

>100_bases
TATTTGCGATTCCAACTGCTTGGGCTCCGCGAATGTTTTCACTCATTTTTTAATCGACCGCTTCCATCATGTTTTAACTA
AGGTTTGTAGGCTTAAACCT

Downstream 100 bases:

>100_bases
CCGCATTCGGGGTTATCGTGGGACTTCCGAAATGTAACTAGAGACTAGAGGAGGAAACACGATGGCTCCTAAACAAACTC
CCAGCCCAGAGAAGAATCGA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNSELKPGLDLLGDPIVLTQRLVDIPSPSGQEKQIADEIEDALRNLNLPGVEVFRFNNNVLARTNRGLASRVMLAGHIDT
VPIADNLPSRVEDGIMYGCGTVDMKSGLAVYLHTFATLATSTELKHDLTLIAYECEEVADHLNGLGHIRDEHPEWLAADL
ALLGEPTGGWIEAGCQGNLRIKVTAHGVRAHSARSWLGDNAMHKLSPIISKVAAYKAAEVNIDGLTYREGLNIVFCESGV
ANNVIPDLAWMNLNFRFAPNRDLNEAIEHVVETLELDGQDGIEWAVEDGAGGALPGLGQQVTSGLIDAVGREKIRAKFGW
TDVSRFSAMGIPALNFGAGDPSFAHKRDEQCPVEQITDVAAILKQYLSE

Sequences:

>Translated_369_residues
MNSELKPGLDLLGDPIVLTQRLVDIPSPSGQEKQIADEIEDALRNLNLPGVEVFRFNNNVLARTNRGLASRVMLAGHIDT
VPIADNLPSRVEDGIMYGCGTVDMKSGLAVYLHTFATLATSTELKHDLTLIAYECEEVADHLNGLGHIRDEHPEWLAADL
ALLGEPTGGWIEAGCQGNLRIKVTAHGVRAHSARSWLGDNAMHKLSPIISKVAAYKAAEVNIDGLTYREGLNIVFCESGV
ANNVIPDLAWMNLNFRFAPNRDLNEAIEHVVETLELDGQDGIEWAVEDGAGGALPGLGQQVTSGLIDAVGREKIRAKFGW
TDVSRFSAMGIPALNFGAGDPSFAHKRDEQCPVEQITDVAAILKQYLSE
>Mature_369_residues
MNSELKPGLDLLGDPIVLTQRLVDIPSPSGQEKQIADEIEDALRNLNLPGVEVFRFNNNVLARTNRGLASRVMLAGHIDT
VPIADNLPSRVEDGIMYGCGTVDMKSGLAVYLHTFATLATSTELKHDLTLIAYECEEVADHLNGLGHIRDEHPEWLAADL
ALLGEPTGGWIEAGCQGNLRIKVTAHGVRAHSARSWLGDNAMHKLSPIISKVAAYKAAEVNIDGLTYREGLNIVFCESGV
ANNVIPDLAWMNLNFRFAPNRDLNEAIEHVVETLELDGQDGIEWAVEDGAGGALPGLGQQVTSGLIDAVGREKIRAKFGW
TDVSRFSAMGIPALNFGAGDPSFAHKRDEQCPVEQITDVAAILKQYLSE

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; fifth step. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family

Homologues:

Organism=Escherichia coli, GI1788816, Length=258, Percent_Identity=27.5193798449612, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790395, Length=377, Percent_Identity=24.4031830238727, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_CORGL (Q59284)

Other databases:

- EMBL:   X81379
- EMBL:   BA000036
- EMBL:   BX927151
- EMBL:   X85965
- PIR:   S60063
- RefSeq:   NP_600337.1
- RefSeq:   YP_225401.1
- ProteinModelPortal:   Q59284
- SMR:   Q59284
- GeneID:   1019094
- GeneID:   3344279
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg1260
- KEGG:   cgl:NCgl1064
- HOGENOM:   HBG292411
- OMA:   VHVNYRF
- ProtClustDB:   PRK13007
- BioCyc:   CGLU196627:CG1260-MONOMER
- BRENDA:   3.5.1.18
- GO:   GO:0006508
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010174
- TIGRFAMs:   TIGR01900

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 39975; Mature: 39975

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: ACT_SITE 79-79 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSELKPGLDLLGDPIVLTQRLVDIPSPSGQEKQIADEIEDALRNLNLPGVEVFRFNNNV
CCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCE
LARTNRGLASRVMLAGHIDTVPIADNLPSRVEDGIMYGCGTVDMKSGLAVYLHTFATLAT
EEECCCCHHHHHHEECCCCCCCCCCCCCHHHHCCEEECCCCCCHHCCHHHHHHHHHHHHH
STELKHDLTLIAYECEEVADHLNGLGHIRDEHPEWLAADLALLGEPTGGWIEAGCQGNLR
HHHHHCCEEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCEE
IKVTAHGVRAHSARSWLGDNAMHKLSPIISKVAAYKAAEVNIDGLTYREGLNIVFCESGV
EEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCEEEECCCC
ANNVIPDLAWMNLNFRFAPNRDLNEAIEHVVETLELDGQDGIEWAVEDGAGGALPGLGQQ
CCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHH
VTSGLIDAVGREKIRAKFGWTDVSRFSAMGIPALNFGAGDPSFAHKRDEQCPVEQITDVA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCCCCCCHHHCCCCCCCHHHHHHHH
AILKQYLSE
HHHHHHHCC
>Mature Secondary Structure
MNSELKPGLDLLGDPIVLTQRLVDIPSPSGQEKQIADEIEDALRNLNLPGVEVFRFNNNV
CCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCE
LARTNRGLASRVMLAGHIDTVPIADNLPSRVEDGIMYGCGTVDMKSGLAVYLHTFATLAT
EEECCCCHHHHHHEECCCCCCCCCCCCCHHHHCCEEECCCCCCHHCCHHHHHHHHHHHHH
STELKHDLTLIAYECEEVADHLNGLGHIRDEHPEWLAADLALLGEPTGGWIEAGCQGNLR
HHHHHCCEEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCEE
IKVTAHGVRAHSARSWLGDNAMHKLSPIISKVAAYKAAEVNIDGLTYREGLNIVFCESGV
EEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCEEEECCCC
ANNVIPDLAWMNLNFRFAPNRDLNEAIEHVVETLELDGQDGIEWAVEDGAGGALPGLGQQ
CCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHH
VTSGLIDAVGREKIRAKFGWTDVSRFSAMGIPALNFGAGDPSFAHKRDEQCPVEQITDVA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCCCCCCHHHCCCCCCCHHHHHHHH
AILKQYLSE
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7881553; 12948626; 7592354