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The map label for this gene is tdcB [H]

Identifier: 62389869

GI number: 62389869

Start: 1040188

End: 1041120

Strand: Reverse

Name: tdcB [H]

Synonym: cg1116

Alternate gene names: 62389869

Gene position: 1041120-1040188 (Counterclockwise)

Preceding gene: 62389875

Following gene: 62389863

Centisome position: 31.72

GC content: 55.41

Gene sequence:

>933_bases
ATGCTCACCCTCAACGATGTCATCACCGCCCAACAACGAACCGCCCCTCATGTTCGACGAACGCCACTTTTCGAAGCAGA
CCCCATCGACGGCACACAAATCTGGATCAAAGCAGAGTTCCTCCAAAAGTGCGGCGTGTTCAAAACGCGTGGAGCATTCA
ACCGCCAGCTCGCAGCTTCGGAAAACGGACTACTCGACCCAACGGTTGGCATCGTCGCGGCATCAGGCGGAAACGCAGGA
CTCGCAAATGCTTTTGCCGCAGCATCCTTAAGCGTTCCCGCCACGGTATTGGTGCCCGAAACTGCCCCACAAGTAAAAGT
TGATCGCCTCAAGCAATACGGTGCAACCGTGCAACAAATCGGATCTGAATATGCGGAAGCATTTGAGGCAGCTCAAACCT
TTGAGTCGGAAACTGGTGCTCTGTTTTGCCACGCCTACGACCAGCCCGACATCGCAGCTGGAGCAGGCGTCATTGGGCTA
GAAATTGTCGAAGATCTTCCCGACGTTGACACCATCGTGGTTGCTGTCGGTGGCGGTGGACTCTATGCAGGAATCGCAGC
CGTCGTAGCAGCCCACGACATCAAAGTGGTGGCCGTTGAACCCTCCAAAATTCCAACCCTGCACAACTCACTCATTGCCG
GCCAACCAGTCGATGTGAACGTTTCTGGTATCGCGGCAGATTCTTTGGGGGCTCGCCAAATTGGACGAGAAGCCTTTGAC
ATCGCAACTGCCCATCCCCCAATAGGCGTCCTAGTGGACGATGAAGCAATCATCGCAGCTCGACGCCACCTCTGGGACAA
CTACCGCATCCCTGCCGAGCATGGCGCTGCCGCAGCACTCGCCTCTCTTACCAGTGGAGCATACAAACCTGCAGCAGATG
AAAAAGTGGCAGTCATTGTGTGCGGAGCGAACACTGACCTCACAACACTGTGA

Upstream 100 bases:

>100_bases
GTGCCCAGAAATTCTGCTTGCACTCACCCAAGCCGTTTAGCAAATTGAACCTCACGTTCATAATAATGTTCATTTTCATC
GAGTTCTAGAAAACACAGGC

Downstream 100 bases:

>100_bases
TGTGATTTCAAACGATCACACGACGAAGAATCTCCACAGCTATCTTCGCCGTGGAGATTTCTTTTTTGGACACTCTCTAG
ACACTTAAGTCTAAGACAGC

Product: threonine dehydratase

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MLTLNDVITAQQRTAPHVRRTPLFEADPIDGTQIWIKAEFLQKCGVFKTRGAFNRQLAASENGLLDPTVGIVAASGGNAG
LANAFAAASLSVPATVLVPETAPQVKVDRLKQYGATVQQIGSEYAEAFEAAQTFESETGALFCHAYDQPDIAAGAGVIGL
EIVEDLPDVDTIVVAVGGGGLYAGIAAVVAAHDIKVVAVEPSKIPTLHNSLIAGQPVDVNVSGIAADSLGARQIGREAFD
IATAHPPIGVLVDDEAIIAARRHLWDNYRIPAEHGAAAALASLTSGAYKPAADEKVAVIVCGANTDLTTL

Sequences:

>Translated_310_residues
MLTLNDVITAQQRTAPHVRRTPLFEADPIDGTQIWIKAEFLQKCGVFKTRGAFNRQLAASENGLLDPTVGIVAASGGNAG
LANAFAAASLSVPATVLVPETAPQVKVDRLKQYGATVQQIGSEYAEAFEAAQTFESETGALFCHAYDQPDIAAGAGVIGL
EIVEDLPDVDTIVVAVGGGGLYAGIAAVVAAHDIKVVAVEPSKIPTLHNSLIAGQPVDVNVSGIAADSLGARQIGREAFD
IATAHPPIGVLVDDEAIIAARRHLWDNYRIPAEHGAAAALASLTSGAYKPAADEKVAVIVCGANTDLTTL
>Mature_310_residues
MLTLNDVITAQQRTAPHVRRTPLFEADPIDGTQIWIKAEFLQKCGVFKTRGAFNRQLAASENGLLDPTVGIVAASGGNAG
LANAFAAASLSVPATVLVPETAPQVKVDRLKQYGATVQQIGSEYAEAFEAAQTFESETGALFCHAYDQPDIAAGAGVIGL
EIVEDLPDVDTIVVAVGGGGLYAGIAAVVAAHDIKVVAVEPSKIPTLHNSLIAGQPVDVNVSGIAADSLGARQIGREAFD
IATAHPPIGVLVDDEAIIAARRHLWDNYRIPAEHGAAAALASLTSGAYKPAADEKVAVIVCGANTDLTTL

Specific function: Catalyzes The Formation Of Alpha-Ketobutyrate From Threonine In A Two-Step Reaction. The First Step Is A Dehydration Of Threonine, Followed By Rehydration And Liberation Of Ammonia. Deaminates L-Threonine, But Also L-Serine To A Lesser Extent. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=322, Percent_Identity=29.1925465838509, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI33469958, Length=311, Percent_Identity=30.2250803858521, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI19923959, Length=300, Percent_Identity=30, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1790207, Length=308, Percent_Identity=31.4935064935065, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789505, Length=314, Percent_Identity=30.2547770700637, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17508781, Length=315, Percent_Identity=29.8412698412698, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71991565, Length=300, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17537387, Length=318, Percent_Identity=31.4465408805031, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6319788, Length=302, Percent_Identity=32.1192052980132, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6322631, Length=313, Percent_Identity=29.3929712460064, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320930, Length=293, Percent_Identity=29.3515358361775, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322024, Length=197, Percent_Identity=28.9340101522843, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355833, Length=315, Percent_Identity=34.2857142857143, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24645328, Length=217, Percent_Identity=35.4838709677419, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 32061; Mature: 32061

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLNDVITAQQRTAPHVRRTPLFEADPIDGTQIWIKAEFLQKCGVFKTRGAFNRQLAAS
CEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCEECCCCCCCCHHCC
ENGLLDPTVGIVAASGGNAGLANAFAAASLSVPATVLVPETAPQVKVDRLKQYGATVQQI
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCHHHHHH
GSEYAEAFEAAQTFESETGALFCHAYDQPDIAAGAGVIGLEIVEDLPDVDTIVVAVGGGG
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCH
LYAGIAAVVAAHDIKVVAVEPSKIPTLHNSLIAGQPVDVNVSGIAADSLGARQIGREAFD
HHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHCCCCCEEEECCEECCCCCHHHHCHHHHH
IATAHPPIGVLVDDEAIIAARRHLWDNYRIPAEHGAAAALASLTSGAYKPAADEKVAVIV
HCCCCCCEEEEECCHHHHHHHHHHHCCCCCCHHHCHHHHHHHHHCCCCCCCCCCEEEEEE
CGANTDLTTL
ECCCCCCCCC
>Mature Secondary Structure
MLTLNDVITAQQRTAPHVRRTPLFEADPIDGTQIWIKAEFLQKCGVFKTRGAFNRQLAAS
CEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCEECCCCCCCCHHCC
ENGLLDPTVGIVAASGGNAGLANAFAAASLSVPATVLVPETAPQVKVDRLKQYGATVQQI
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCHHHHHH
GSEYAEAFEAAQTFESETGALFCHAYDQPDIAAGAGVIGLEIVEDLPDVDTIVVAVGGGG
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCH
LYAGIAAVVAAHDIKVVAVEPSKIPTLHNSLIAGQPVDVNVSGIAADSLGARQIGREAFD
HHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHCCCCCEEEECCEECCCCCHHHHCHHHHH
IATAHPPIGVLVDDEAIIAARRHLWDNYRIPAEHGAAAALASLTSGAYKPAADEKVAVIV
HCCCCCCEEEEECCHHHHHHHHHHHCCCCCCHHHCHHHHHHHHHCCCCCCCCCCEEEEEE
CGANTDLTTL
ECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]