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The map label for this gene is prsA [H]

Identifier: 62389833

GI number: 62389833

Start: 998933

End: 999910

Strand: Reverse

Name: prsA [H]

Synonym: cg1075

Alternate gene names: 62389833

Gene position: 999910-998933 (Counterclockwise)

Preceding gene: 62389834

Following gene: 62389832

Centisome position: 30.46

GC content: 59.51

Gene sequence:

>978_bases
ATGACTGCTCACTGGAAACAAAACCAAAAGAACCTCATGCTGTTTTCGGGTCGTGCGCACCCAGAACTGGCAGAAGCTGT
AGCTAAAGAGCTCGACGTCAACGTCACCCCAATGACGGCACGCGATTTCGCCAACGGTGAAATCTACGTCCGCTTCGAGG
AATCAGTTCGTGGCTCCGACTGCTTCGTCCTGCAGTCCCACACCCAGCCTCTCAACAAGTGGCTCATGGAACAGCTGCTG
ATGATCGACGCTTTGAAGCGTGGTTCCGCAAAGCGCATCACCGCGATCCTGCCGTTCTACCCATATGCCCGCCAGGACAA
GAAGCACCGCGGCCGCGAGCCAATTTCTGCTCGCCTCATCGCCGACCTCATGCTCACCGCTGGCGCGGACCGTATCGTGT
CCGTGGACTTGCACACCGATCAGATCCAGGGCTTCTTCGACGGCCCAGTCGATCACATGCACGCCATGCCGATCCTCACC
GATCACATCAAGGAAAACTACAACCTGGACAACATCTGCGTGGTCTCCCCTGACGCAGGTCGCGTGAAGGTTGCAGAGAA
GTGGGCTAACACCTTGGGCGATGCCCCAATGGCGTTCGTGCACAAGACCCGCTCCACCGAGGTAGCAAACCAGGTTGTCG
CCAACCGCGTCGTCGGTGACGTCGACGGCAAGGACTGCGTGCTTCTCGACGACATGATCGACACTGGCGGCACCATCGCC
GGCGCTGTGGGCGTCCTGAAGAAGGCTGGCGCAAAGTCAGTCGTCATCGCCTGCACCCACGGTGTGTTCTCTGACCCAGC
CCGCGAGCGCCTGTCTGCATGCGGTGCTGAAGAAGTCATCACCACCGACACCCTGCCACAGTCCACCGAGGGCTGGAGCA
ACCTGACCGTTTTGTCGATCGCACCGCTGCTGGCTCGCACCATCAACGAGATCTTCGAAAACGGTTCCGTCACCACCCTC
TTCGAGGGCGAGGCCTAA

Upstream 100 bases:

>100_bases
TCGAAGGCTGGGTGCAAAAGAAGCGCCCTGGAACCGCTGCAGCACAAGCCGCAGAAGCCGCCCAAAACGTCCACAACCAG
GAAGGCTAAGCAGGATCCTC

Downstream 100 bases:

>100_bases
ACACCCATGCCCACCACGGACGTCTTCAACCGCGTCCGGTTGGCATTGGAACCTCTAGCTGATCCCGCACGTGCCACCGG
AATGGCAAGCTACATGCGGG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTAHWKQNQKNLMLFSGRAHPELAEAVAKELDVNVTPMTARDFANGEIYVRFEESVRGSDCFVLQSHTQPLNKWLMEQLL
MIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARLIADLMLTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPILT
DHIKENYNLDNICVVSPDAGRVKVAEKWANTLGDAPMAFVHKTRSTEVANQVVANRVVGDVDGKDCVLLDDMIDTGGTIA
GAVGVLKKAGAKSVVIACTHGVFSDPARERLSACGAEEVITTDTLPQSTEGWSNLTVLSIAPLLARTINEIFENGSVTTL
FEGEA

Sequences:

>Translated_325_residues
MTAHWKQNQKNLMLFSGRAHPELAEAVAKELDVNVTPMTARDFANGEIYVRFEESVRGSDCFVLQSHTQPLNKWLMEQLL
MIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARLIADLMLTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPILT
DHIKENYNLDNICVVSPDAGRVKVAEKWANTLGDAPMAFVHKTRSTEVANQVVANRVVGDVDGKDCVLLDDMIDTGGTIA
GAVGVLKKAGAKSVVIACTHGVFSDPARERLSACGAEEVITTDTLPQSTEGWSNLTVLSIAPLLARTINEIFENGSVTTL
FEGEA
>Mature_324_residues
TAHWKQNQKNLMLFSGRAHPELAEAVAKELDVNVTPMTARDFANGEIYVRFEESVRGSDCFVLQSHTQPLNKWLMEQLLM
IDALKRGSAKRITAILPFYPYARQDKKHRGREPISARLIADLMLTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPILTD
HIKENYNLDNICVVSPDAGRVKVAEKWANTLGDAPMAFVHKTRSTEVANQVVANRVVGDVDGKDCVLLDDMIDTGGTIAG
AVGVLKKAGAKSVVIACTHGVFSDPARERLSACGAEEVITTDTLPQSTEGWSNLTVLSIAPLLARTINEIFENGSVTTLF
EGEA

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=42.3566878980892, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=42.0382165605096, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=42.3566878980892, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI84875539, Length=317, Percent_Identity=41.9558359621451, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI4506133, Length=346, Percent_Identity=30.635838150289, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI194018537, Length=332, Percent_Identity=31.9277108433735, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI310128524, Length=145, Percent_Identity=29.6551724137931, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI310115209, Length=145, Percent_Identity=29.6551724137931, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI310118259, Length=145, Percent_Identity=29.6551724137931, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI310119946, Length=145, Percent_Identity=29.6551724137931, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=43.8095238095238, Blast_Score=259, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=42.3566878980892, Blast_Score=248, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=42.3566878980892, Blast_Score=247, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=42.3566878980892, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17554704, Length=311, Percent_Identity=42.443729903537, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=32.5443786982249, Blast_Score=180, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6320946, Length=313, Percent_Identity=39.6166134185303, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6319403, Length=314, Percent_Identity=39.4904458598726, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6321776, Length=316, Percent_Identity=40.8227848101266, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6322667, Length=201, Percent_Identity=39.8009950248756, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6324511, Length=95, Percent_Identity=36.8421052631579, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355239, Length=314, Percent_Identity=41.7197452229299, Blast_Score=241, Evalue=7e-64,
Organism=Drosophila melanogaster, GI45551540, Length=337, Percent_Identity=39.1691394658754, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24651458, Length=355, Percent_Identity=29.0140845070423, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24651456, Length=355, Percent_Identity=29.0140845070423, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI281362873, Length=357, Percent_Identity=28.8515406162465, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24651454, Length=357, Percent_Identity=28.8515406162465, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24651462, Length=203, Percent_Identity=33.9901477832512, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24651464, Length=203, Percent_Identity=33.9901477832512, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45552010, Length=203, Percent_Identity=33.9901477832512, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 35551; Mature: 35420

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHWKQNQKNLMLFSGRAHPELAEAVAKELDVNVTPMTARDFANGEIYVRFEESVRGSD
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEECCCCCCCC
CFVLQSHTQPLNKWLMEQLLMIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARLI
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCHHHHHH
ADLMLTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPILTDHIKENYNLDNICVVSPDAG
HHHHHHCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
RVKVAEKWANTLGDAPMAFVHKTRSTEVANQVVANRVVGDVDGKDCVLLDDMIDTGGTIA
CEEHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCHHH
GAVGVLKKAGAKSVVIACTHGVFSDPARERLSACGAEEVITTDTLPQSTEGWSNLTVLSI
HHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHH
APLLARTINEIFENGSVTTLFEGEA
HHHHHHHHHHHHHCCCEEEEECCCC
>Mature Secondary Structure 
TAHWKQNQKNLMLFSGRAHPELAEAVAKELDVNVTPMTARDFANGEIYVRFEESVRGSD
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEECCCCCCCC
CFVLQSHTQPLNKWLMEQLLMIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARLI
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCHHHHHH
ADLMLTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPILTDHIKENYNLDNICVVSPDAG
HHHHHHCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
RVKVAEKWANTLGDAPMAFVHKTRSTEVANQVVANRVVGDVDGKDCVLLDDMIDTGGTIA
CEEHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCHHH
GAVGVLKKAGAKSVVIACTHGVFSDPARERLSACGAEEVITTDTLPQSTEGWSNLTVLSI
HHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHH
APLLARTINEIFENGSVTTLFEGEA
HHHHHHHHHHHHHCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]