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The map label for this gene is metS [H]

Identifier: 62389783

GI number: 62389783

Start: 948309

End: 950141

Strand: Direct

Name: metS [H]

Synonym: cg1017

Alternate gene names: 62389783

Gene position: 948309-950141 (Clockwise)

Preceding gene: 62389782

Following gene: 62389784

Centisome position: 28.89

GC content: 56.68

Gene sequence:

>1833_bases
ATGACGAAGAACGTGCTCGTATCTGTTGCCTGGCCGTATGCCAACGGACCCCGTCACATTGGACATGTGGCGGGGTTTGG
TGTCCCCTCCGATGTGTTCGCAAGGTTCCAGCGAATGTCTGGCAACAACGTGCTCATGGTGTCCGGCACCGATGAGCACG
GCACGCCACTTCTGGTTCAAGCAGACAAAGAAGGCGTCACCGTTCAAGACCTAGCGGATAAGTACAACCGCCAGATCGTC
GAAGACCTCACCGGCCTGGGCCTGTCCTATGACCTTTTCACCCGCACCACCACCTCCAACCACTACGCAGTAGTGCAGGA
ACTGTTCCGTGGTCTGTACGACAACGGTTACATGATCAAGGAAACCACCCTCGGTGCGATTTCCCCATCCACTGGCCGTA
CCCTGCCAGACCGCTACATTGAAGGCACCTGCCCAATCTGTGGCACCGACGGCGCTCGTGGCGACCAGTGCGACAACTGC
GGAAACCAGCTCGATCCAGCGGACCTGATCAACCCGGTGTCCAAGATCAACGGCGAAACCCCAGAGTTCGTTGAGACCGA
ACACTTCCTGCTCGACCTGCCAGCACTGGCTGAAGCACTAACCGAGTGGCTGAAGGGACGCGAAGACTGGCGTCCAAACG
TGTTGAAGTTCTCGCTCAACCTGCTGGACGATATCCGCCCACGCGCAATGTCGCGCGATATCGACTGGGGCATCCCAATC
CCAGTTGAAGGATGGCAAGACAACAACGCCAAGAAGCTCTACGTCTGGTTCGACGCTGTCGTGGGCTACTTGTCCGCATC
CATCGAATGGGCCTACCGCTCCGGCGACCCAGAAGCATGGCGCACCTTCTGGAATGATCCAGAAACCAAGTCCTACTACT
TCATGGGCAAAGACAACATCACCTTCCACTCCCAGATCTGGCCAGCGGAGCTTCTCGGCTACGCAGGCAAGGGCTCCCGC
GGTGGAGAAATCGGTGACCTGGGTGTTCTGAACCTGCCTACTGAGGTTGTTTCCTCTGAGTTCCTGACTATGTCTGGATC
CAAGTTCTCCTCATCCAAGGGCGTTGTCATCTACGTGAAGGACTTCCTCAAGGAGTTCGGCCCAGATGCGCTGCGATACT
TCATCGCTGTCGCAGGCCCAGAAAACAACGACACCGACTTCACCTGGGATGAATTTGTCCGCCGCGTAAATAACGAGCTG
GCAAACGGCTGGGGCAACCTGGTCAACCGCACTGTATCCATGGCGCACAAGAACTTCGGTGAAGTACCAGTACCTGGCGC
ACTGGAAGAATCTGACAAGAAGATCCTTGATCTTGCTACCGCTGCCTTTGAATCCGTTGCTGCGAACCTGGATCAGTCCA
AGTTCAAGGCCGGTATCTCTGAAATCATGCACGTTGTCGGTGAGGCCAACGCCTACATCGCAGAGCAAGAACCATGGAAG
CTTGCCAAGGATGACACCAAGCGCGAGCGTCTTGCCACCGTGCTGTGGACTGCGCTGCAGGTTGTTTCTGACTGCAACAC
CATGCTGACCCCATACCTGCCACACACCGCCCAAAAGGTGCATGAGACCTTGGGCCGTGATGGAATCTGGGCTGCAACAC
CACAGATCGTGGAAGTCACCAACGAATCACCACGCCAGCCAATCGGCGTGGGGCTACCAGATCCAGAGCACACCTACCCA
GTAATCATGGGCGACTACAAGACCCAGCTGGCTAAGTGGCAGCGCATCGACGTTGTGCCAGGCACCACCTTGGAGAAGCC
AGCACCGCTGATTGCTAAGCTCGATCCAGAACTTGGTGAAACCGGCCCAGAATGGGCACCAGTGCAGAACTAA

Upstream 100 bases:

>100_bases
GACGCAGGGAGCGTAAGGCGAGTGGCGCGGGGAAGCGTCGATAGGCAATTTTTAACCCCTGATACCCCTTTCCGGCCGGG
CATAAATTAAGGTGGTACGC

Downstream 100 bases:

>100_bases
AGCATCTTTAGCATGAACCGAGCAGGTTGATCAGAAACATCTGATTGACCTGCTCTTATTAGTTGGCGGGGGTGGGGCGG
GGGAGTGTGTGTCCACCCCG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MTKNVLVSVAWPYANGPRHIGHVAGFGVPSDVFARFQRMSGNNVLMVSGTDEHGTPLLVQADKEGVTVQDLADKYNRQIV
EDLTGLGLSYDLFTRTTTSNHYAVVQELFRGLYDNGYMIKETTLGAISPSTGRTLPDRYIEGTCPICGTDGARGDQCDNC
GNQLDPADLINPVSKINGETPEFVETEHFLLDLPALAEALTEWLKGREDWRPNVLKFSLNLLDDIRPRAMSRDIDWGIPI
PVEGWQDNNAKKLYVWFDAVVGYLSASIEWAYRSGDPEAWRTFWNDPETKSYYFMGKDNITFHSQIWPAELLGYAGKGSR
GGEIGDLGVLNLPTEVVSSEFLTMSGSKFSSSKGVVIYVKDFLKEFGPDALRYFIAVAGPENNDTDFTWDEFVRRVNNEL
ANGWGNLVNRTVSMAHKNFGEVPVPGALEESDKKILDLATAAFESVAANLDQSKFKAGISEIMHVVGEANAYIAEQEPWK
LAKDDTKRERLATVLWTALQVVSDCNTMLTPYLPHTAQKVHETLGRDGIWAATPQIVEVTNESPRQPIGVGLPDPEHTYP
VIMGDYKTQLAKWQRIDVVPGTTLEKPAPLIAKLDPELGETGPEWAPVQN

Sequences:

>Translated_610_residues
MTKNVLVSVAWPYANGPRHIGHVAGFGVPSDVFARFQRMSGNNVLMVSGTDEHGTPLLVQADKEGVTVQDLADKYNRQIV
EDLTGLGLSYDLFTRTTTSNHYAVVQELFRGLYDNGYMIKETTLGAISPSTGRTLPDRYIEGTCPICGTDGARGDQCDNC
GNQLDPADLINPVSKINGETPEFVETEHFLLDLPALAEALTEWLKGREDWRPNVLKFSLNLLDDIRPRAMSRDIDWGIPI
PVEGWQDNNAKKLYVWFDAVVGYLSASIEWAYRSGDPEAWRTFWNDPETKSYYFMGKDNITFHSQIWPAELLGYAGKGSR
GGEIGDLGVLNLPTEVVSSEFLTMSGSKFSSSKGVVIYVKDFLKEFGPDALRYFIAVAGPENNDTDFTWDEFVRRVNNEL
ANGWGNLVNRTVSMAHKNFGEVPVPGALEESDKKILDLATAAFESVAANLDQSKFKAGISEIMHVVGEANAYIAEQEPWK
LAKDDTKRERLATVLWTALQVVSDCNTMLTPYLPHTAQKVHETLGRDGIWAATPQIVEVTNESPRQPIGVGLPDPEHTYP
VIMGDYKTQLAKWQRIDVVPGTTLEKPAPLIAKLDPELGETGPEWAPVQN
>Mature_609_residues
TKNVLVSVAWPYANGPRHIGHVAGFGVPSDVFARFQRMSGNNVLMVSGTDEHGTPLLVQADKEGVTVQDLADKYNRQIVE
DLTGLGLSYDLFTRTTTSNHYAVVQELFRGLYDNGYMIKETTLGAISPSTGRTLPDRYIEGTCPICGTDGARGDQCDNCG
NQLDPADLINPVSKINGETPEFVETEHFLLDLPALAEALTEWLKGREDWRPNVLKFSLNLLDDIRPRAMSRDIDWGIPIP
VEGWQDNNAKKLYVWFDAVVGYLSASIEWAYRSGDPEAWRTFWNDPETKSYYFMGKDNITFHSQIWPAELLGYAGKGSRG
GEIGDLGVLNLPTEVVSSEFLTMSGSKFSSSKGVVIYVKDFLKEFGPDALRYFIAVAGPENNDTDFTWDEFVRRVNNELA
NGWGNLVNRTVSMAHKNFGEVPVPGALEESDKKILDLATAAFESVAANLDQSKFKAGISEIMHVVGEANAYIAEQEPWKL
AKDDTKRERLATVLWTALQVVSDCNTMLTPYLPHTAQKVHETLGRDGIWAATPQIVEVTNESPRQPIGVGLPDPEHTYPV
IMGDYKTQLAKWQRIDVVPGTTLEKPAPLIAKLDPELGETGPEWAPVQN

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=531, Percent_Identity=33.3333333333333, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI24308436, Length=539, Percent_Identity=25.974025974026, Blast_Score=162, Evalue=1e-39,
Organism=Escherichia coli, GI1788432, Length=539, Percent_Identity=29.8701298701299, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1786861, Length=153, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=597, Percent_Identity=30.1507537688442, Blast_Score=295, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI212645096, Length=527, Percent_Identity=26.5654648956357, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71992781, Length=363, Percent_Identity=26.4462809917355, Blast_Score=122, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321704, Length=536, Percent_Identity=32.4626865671642, Blast_Score=306, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6321610, Length=549, Percent_Identity=22.7686703096539, Blast_Score=106, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24655311, Length=529, Percent_Identity=32.5141776937618, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI21358483, Length=564, Percent_Identity=25.177304964539, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 67620; Mature: 67488

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNVLVSVAWPYANGPRHIGHVAGFGVPSDVFARFQRMSGNNVLMVSGTDEHGTPLLVQ
CCCCEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEE
ADKEGVTVQDLADKYNRQIVEDLTGLGLSYDLFTRTTTSNHYAVVQELFRGLYDNGYMIK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHEEECCCCCHHHHHHHHHHHHCCCCEEEE
ETTLGAISPSTGRTLPDRYIEGTCPICGTDGARGDQCDNCGNQLDPADLINPVSKINGET
ECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PEFVETEHFLLDLPALAEALTEWLKGREDWRPNVLKFSLNLLDDIRPRAMSRDIDWGIPI
CCCCCCHHHEEECHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCCHHHHCCCCCCCCCC
PVEGWQDNNAKKLYVWFDAVVGYLSASIEWAYRSGDPEAWRTFWNDPETKSYYFMGKDNI
CCCCCCCCCCCEEEEEHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCCCCEEEEECCCCE
TFHSQIWPAELLGYAGKGSRGGEIGDLGVLNLPTEVVSSEFLTMSGSKFSSSKGVVIYVK
EEECCCCHHHHHCCCCCCCCCCCCCCCCEECCCHHHHCCHHEEECCCCCCCCCCEEEEHH
DFLKEFGPDALRYFIAVAGPENNDTDFTWDEFVRRVNNELANGWGNLVNRTVSMAHKNFG
HHHHHHCHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVPVPGALEESDKKILDLATAAFESVAANLDQSKFKAGISEIMHVVGEANAYIAEQEPWK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCC
LAKDDTKRERLATVLWTALQVVSDCNTMLTPYLPHTAQKVHETLGRDGIWAATPQIVEVT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCEEEEC
NESPRQPIGVGLPDPEHTYPVIMGDYKTQLAKWQRIDVVPGTTLEKPAPLIAKLDPELGE
CCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHEEECCCCCCCCCCCCHHEECCCHHCC
TGPEWAPVQN
CCCCCCCCCC
>Mature Secondary Structure 
TKNVLVSVAWPYANGPRHIGHVAGFGVPSDVFARFQRMSGNNVLMVSGTDEHGTPLLVQ
CCCEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEE
ADKEGVTVQDLADKYNRQIVEDLTGLGLSYDLFTRTTTSNHYAVVQELFRGLYDNGYMIK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHEEECCCCCHHHHHHHHHHHHCCCCEEEE
ETTLGAISPSTGRTLPDRYIEGTCPICGTDGARGDQCDNCGNQLDPADLINPVSKINGET
ECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PEFVETEHFLLDLPALAEALTEWLKGREDWRPNVLKFSLNLLDDIRPRAMSRDIDWGIPI
CCCCCCHHHEEECHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCCHHHHCCCCCCCCCC
PVEGWQDNNAKKLYVWFDAVVGYLSASIEWAYRSGDPEAWRTFWNDPETKSYYFMGKDNI
CCCCCCCCCCCEEEEEHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCCCCEEEEECCCCE
TFHSQIWPAELLGYAGKGSRGGEIGDLGVLNLPTEVVSSEFLTMSGSKFSSSKGVVIYVK
EEECCCCHHHHHCCCCCCCCCCCCCCCCEECCCHHHHCCHHEEECCCCCCCCCCEEEEHH
DFLKEFGPDALRYFIAVAGPENNDTDFTWDEFVRRVNNELANGWGNLVNRTVSMAHKNFG
HHHHHHCHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVPVPGALEESDKKILDLATAAFESVAANLDQSKFKAGISEIMHVVGEANAYIAEQEPWK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCC
LAKDDTKRERLATVLWTALQVVSDCNTMLTPYLPHTAQKVHETLGRDGIWAATPQIVEVT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCEEEEC
NESPRQPIGVGLPDPEHTYPVIMGDYKTQLAKWQRIDVVPGTTLEKPAPLIAKLDPELGE
CCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHEEECCCCCCCCCCCCHHEECCCHHCC
TGPEWAPVQN
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]