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The map label for this gene is yvkC [H]

Identifier: 62389442

GI number: 62389442

Start: 563731

End: 565638

Strand: Reverse

Name: yvkC [H]

Synonym: cg0642

Alternate gene names: 62389442

Gene position: 565638-563731 (Counterclockwise)

Preceding gene: 62389443

Following gene: 62389441

Centisome position: 17.23

GC content: 52.15

Gene sequence:

>1908_bases
TTGACCATGGCTAATAAATCTTTCCCCAAGCCCTCCGATCTTCCAGTGCCCAAGGGCGCTGAAGGTTGGGAAGATCTGTA
CCCGTACTACCTCGTTTTCCAAGACAAGCTCATGGATCAAGAGAATGAGAAATTCTGGTTCTGCGATTCACAGCACTGGC
CAACTGTGTTCAAGCCTTTTGAAACTATCGGTGGTGAATTCGCTGTAAAGTGCCTCGGCCAATACAACGCTCGGCATTTG
ATGATCCCGAATGCCAATGGCATCGAGTTCCGCGTGCATCTGGGATACCTCTATATGTCCCCTATTCCAGTGCCTGAAGA
TCAGATTGCGGAACGCGTCCCCATGTTCCAGGAACGCATCACGCACTACTTCCAAAACTGGGAGCCAATGCTGGCAAATT
GGAAGGAGCGAGTATTAGGAACCATCAATGAGCTGGAATCTCTAGAATTCAAGCCACTGCCTGACTACGTGCCTATCGAT
GATATTGTCTCCGGAAAAGCCAAAGACGGCACCGAAGTACTCATGGAAAACTTCGATCGGCTCATTCAGCTCGCCTACCA
AAACTGGCAATACCACTTTGAGTTCCTCAACTTGGGTTACATCGCTTACCTAGATTTCTTCAATTTCTGCAAGGAAGTCT
TCCCAGATATCCCTGATCAATCAATTTCGATGATGGTTCAGGGCGTGGATATGGAGCTGTTCCGCCCCGATGATGAACTA
AAGATTCTGGCACAGCTAGCGGTCGACCTTGGCCTGCAAACTCACTTTGCCAACCCGGATGATCCGCAAGCTACCTTGGC
TGCTATCGCAAAGGCAGAAGGCGGCGCGACATGGATAGCGCGCTGGGAAGAAGCACAAGATCCGTGGTTCAACTTCACCG
TCGGTAATGGCTTCTACGGTCACGATAAATACTGGATCGAGCACCTGGAACTTCCACTGGGGTACATCGCGGATTACATC
CGCCGCCTAGATGAAGGCCAAACCATCTCCCGCCCGAAAGATGAACTCATCGCAGAAAAGGAACGCGTGGTGGAAGAATA
CCGCGACCTTTTGGATGGAGAACAACTCGCGCAGTTTGATGCTAAATGCGGCCTCGCTGCTACTGCATACCCCTATGTGG
AAAACCATAACTTCTACATCGAGCACTGGACCATGTCAGTATTTTGGCGCAAAGTACGCGAACTTTCCCGCACTCTCCAG
GGCTACGGTTTCTGGGAGAACGAGGATGACATGTTGTACCTCAACCGCACTGAAGTCCGCGATGTCCTCTTCGACCTGGC
TACTGCGTGGGGTGTCGGCGCACCCGGTGGTCCAATTGGCACGATCATTTGGCCGGAAGAAATTGAGCGAAGAAAAGCAA
TTGTCACCGCTTTGAAAACTGCCCGACCAGCGCCAGCTCTTAACACTCCTCCAGAGTCCATCACCGAACCTTTCACCCGC
ATGCTCTGGGGAATCACCACCGAACAGGTGCAATCATGGTTGGGCAATGACGAGGATGCCGAAGAAGGAACCCTTAAAGG
CATGGCTGCATCCCCTGGTGTGGTGGAAGGCTACGCTCGAGTAATTCTCAGCGCAGATGACCTTTCAGAAATCCAGCAGG
ATGAAATCCTCGTTGCCCCTGTAACAGCACCTTCTTGGGGCCCAATCTTTGGCAAAATCAAGGCAACAGTCACTGATATT
GGTGGCATGATGAGCCATGCTGCGATCGTGTGCCGCGAATACGGCTTGCCGGCTGTTACTGGAACTGGCGCTGCATCCAC
CACCATCAAAACCGGCGATTACCTCAAGGTCGATGGAACCAAGGGCAAGGTTGTCATTGTTGATCCAGATGCGCCACGCA
TCGAAGGACCCGGCGCGCACAGCCATGCGCACTCAGTAGCAGCACATGGGGTGGATACACATGCCTAG

Upstream 100 bases:

>100_bases
AAAACCATAGGCACCTTCGATTTCAGCTCAATCACCGTCGCAATGACCGGCACGAAGTAAAACCACCGCATCTTTTCGTC
GAAAAGCATCTAAAAGGAGT

Downstream 100 bases:

>100_bases
TCCACGCACTGTTCTTATCACTGGTGCCGCTGGCGGTTTGGGTCGGGCATTCGCTGAAGGTTTCGCAGCCCAAGGAGACC
GTATCGCGGTGGCGGATATC

Product: hypothetical protein

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MTMANKSFPKPSDLPVPKGAEGWEDLYPYYLVFQDKLMDQENEKFWFCDSQHWPTVFKPFETIGGEFAVKCLGQYNARHL
MIPNANGIEFRVHLGYLYMSPIPVPEDQIAERVPMFQERITHYFQNWEPMLANWKERVLGTINELESLEFKPLPDYVPID
DIVSGKAKDGTEVLMENFDRLIQLAYQNWQYHFEFLNLGYIAYLDFFNFCKEVFPDIPDQSISMMVQGVDMELFRPDDEL
KILAQLAVDLGLQTHFANPDDPQATLAAIAKAEGGATWIARWEEAQDPWFNFTVGNGFYGHDKYWIEHLELPLGYIADYI
RRLDEGQTISRPKDELIAEKERVVEEYRDLLDGEQLAQFDAKCGLAATAYPYVENHNFYIEHWTMSVFWRKVRELSRTLQ
GYGFWENEDDMLYLNRTEVRDVLFDLATAWGVGAPGGPIGTIIWPEEIERRKAIVTALKTARPAPALNTPPESITEPFTR
MLWGITTEQVQSWLGNDEDAEEGTLKGMAASPGVVEGYARVILSADDLSEIQQDEILVAPVTAPSWGPIFGKIKATVTDI
GGMMSHAAIVCREYGLPAVTGTGAASTTIKTGDYLKVDGTKGKVVIVDPDAPRIEGPGAHSHAHSVAAHGVDTHA

Sequences:

>Translated_635_residues
MTMANKSFPKPSDLPVPKGAEGWEDLYPYYLVFQDKLMDQENEKFWFCDSQHWPTVFKPFETIGGEFAVKCLGQYNARHL
MIPNANGIEFRVHLGYLYMSPIPVPEDQIAERVPMFQERITHYFQNWEPMLANWKERVLGTINELESLEFKPLPDYVPID
DIVSGKAKDGTEVLMENFDRLIQLAYQNWQYHFEFLNLGYIAYLDFFNFCKEVFPDIPDQSISMMVQGVDMELFRPDDEL
KILAQLAVDLGLQTHFANPDDPQATLAAIAKAEGGATWIARWEEAQDPWFNFTVGNGFYGHDKYWIEHLELPLGYIADYI
RRLDEGQTISRPKDELIAEKERVVEEYRDLLDGEQLAQFDAKCGLAATAYPYVENHNFYIEHWTMSVFWRKVRELSRTLQ
GYGFWENEDDMLYLNRTEVRDVLFDLATAWGVGAPGGPIGTIIWPEEIERRKAIVTALKTARPAPALNTPPESITEPFTR
MLWGITTEQVQSWLGNDEDAEEGTLKGMAASPGVVEGYARVILSADDLSEIQQDEILVAPVTAPSWGPIFGKIKATVTDI
GGMMSHAAIVCREYGLPAVTGTGAASTTIKTGDYLKVDGTKGKVVIVDPDAPRIEGPGAHSHAHSVAAHGVDTHA
>Mature_634_residues
TMANKSFPKPSDLPVPKGAEGWEDLYPYYLVFQDKLMDQENEKFWFCDSQHWPTVFKPFETIGGEFAVKCLGQYNARHLM
IPNANGIEFRVHLGYLYMSPIPVPEDQIAERVPMFQERITHYFQNWEPMLANWKERVLGTINELESLEFKPLPDYVPIDD
IVSGKAKDGTEVLMENFDRLIQLAYQNWQYHFEFLNLGYIAYLDFFNFCKEVFPDIPDQSISMMVQGVDMELFRPDDELK
ILAQLAVDLGLQTHFANPDDPQATLAAIAKAEGGATWIARWEEAQDPWFNFTVGNGFYGHDKYWIEHLELPLGYIADYIR
RLDEGQTISRPKDELIAEKERVVEEYRDLLDGEQLAQFDAKCGLAATAYPYVENHNFYIEHWTMSVFWRKVRELSRTLQG
YGFWENEDDMLYLNRTEVRDVLFDLATAWGVGAPGGPIGTIIWPEEIERRKAIVTALKTARPAPALNTPPESITEPFTRM
LWGITTEQVQSWLGNDEDAEEGTLKGMAASPGVVEGYARVILSADDLSEIQQDEILVAPVTAPSWGPIFGKIKATVTDIG
GMMSHAAIVCREYGLPAVTGTGAASTTIKTGDYLKVDGTKGKVVIVDPDAPRIEGPGAHSHAHSVAAHGVDTHA

Specific function: ESSENTIAL STEP IN GLUCONEOGENESIS WHEN PYRUVATE AND LACTATE ARE USED AS A CARBON SOURCE. [C]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17564524, Length=115, Percent_Identity=36.5217391304348, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2

Molecular weight: Translated: 71709; Mature: 71578

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMANKSFPKPSDLPVPKGAEGWEDLYPYYLVFQDKLMDQENEKFWFCDSQHWPTVFKPF
CCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEEHHHCCCCCCEEEEECCCCCCHHHHHH
ETIGGEFAVKCLGQYNARHLMIPNANGIEFRVHLGYLYMSPIPVPEDQIAERVPMFQERI
HHCCHHHHHHHHHCCCCCEEEECCCCCEEEEEEEEEEEECCCCCCHHHHHHHCHHHHHHH
THYFQNWEPMLANWKERVLGTINELESLEFKPLPDYVPIDDIVSGKAKDGTEVLMENFDR
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHH
LIQLAYQNWQYHFEFLNLGYIAYLDFFNFCKEVFPDIPDQSISMMVQGVDMELFRPDDEL
HHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEECCCHHH
KILAQLAVDLGLQTHFANPDDPQATLAAIAKAEGGATWIARWEEAQDPWFNFTVGNGFYG
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCC
HDKYWIEHLELPLGYIADYIRRLDEGQTISRPKDELIAEKERVVEEYRDLLDGEQLAQFD
CHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
AKCGLAATAYPYVENHNFYIEHWTMSVFWRKVRELSRTLQGYGFWENEDDMLYLNRTEVR
HCCCCEEEECCEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHH
DVLFDLATAWGVGAPGGPIGTIIWPEEIERRKAIVTALKTARPAPALNTPPESITEPFTR
HHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
MLWGITTEQVQSWLGNDEDAEEGTLKGMAASPGVVEGYARVILSADDLSEIQQDEILVAP
HHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHEECCHHHHHHCCCCEEEEE
VTAPSWGPIFGKIKATVTDIGGMMSHAAIVCREYGLPAVTGTGAASTTIKTGDYLKVDGT
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEECCCEEEECCC
KGKVVIVDPDAPRIEGPGAHSHAHSVAAHGVDTHA
CCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
TMANKSFPKPSDLPVPKGAEGWEDLYPYYLVFQDKLMDQENEKFWFCDSQHWPTVFKPF
CCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEEHHHCCCCCCEEEEECCCCCCHHHHHH
ETIGGEFAVKCLGQYNARHLMIPNANGIEFRVHLGYLYMSPIPVPEDQIAERVPMFQERI
HHCCHHHHHHHHHCCCCCEEEECCCCCEEEEEEEEEEEECCCCCCHHHHHHHCHHHHHHH
THYFQNWEPMLANWKERVLGTINELESLEFKPLPDYVPIDDIVSGKAKDGTEVLMENFDR
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHH
LIQLAYQNWQYHFEFLNLGYIAYLDFFNFCKEVFPDIPDQSISMMVQGVDMELFRPDDEL
HHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEECCCHHH
KILAQLAVDLGLQTHFANPDDPQATLAAIAKAEGGATWIARWEEAQDPWFNFTVGNGFYG
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCC
HDKYWIEHLELPLGYIADYIRRLDEGQTISRPKDELIAEKERVVEEYRDLLDGEQLAQFD
CHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
AKCGLAATAYPYVENHNFYIEHWTMSVFWRKVRELSRTLQGYGFWENEDDMLYLNRTEVR
HCCCCEEEECCEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHH
DVLFDLATAWGVGAPGGPIGTIIWPEEIERRKAIVTALKTARPAPALNTPPESITEPFTR
HHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
MLWGITTEQVQSWLGNDEDAEEGTLKGMAASPGVVEGYARVILSADDLSEIQQDEILVAP
HHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHEECCHHHHHHCCCCEEEEE
VTAPSWGPIFGKIKATVTDIGGMMSHAAIVCREYGLPAVTGTGAASTTIKTGDYLKVDGT
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEECCCEEEECCC
KGKVVIVDPDAPRIEGPGAHSHAHSVAAHGVDTHA
CCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.083 {pyruvate}} 0.028 {ATP}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]