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The map label for this gene is ispB [H]

Identifier: 62389371

GI number: 62389371

Start: 494060

End: 495112

Strand: Direct

Name: ispB [H]

Synonym: cg0559

Alternate gene names: 62389371

Gene position: 494060-495112 (Clockwise)

Preceding gene: 62389369

Following gene: 62389372

Centisome position: 15.05

GC content: 59.07

Gene sequence:

>1053_bases
ATGAGTAGCGGCCGAACCGTTCCAACCCGTTCCCACGGGCTCGGAAAAGAAGGTGTATCCACCACAGGAGCATCTCAGGT
CGAGTTTGGTGATCCCGAGCTAACGGCCAGGATCAATGACGCCATGGTGCAGGTAGAAGAACTCCTGCACACTGAACTAT
CGTCCGGGGAAGACTTCCTCGTCGATATCGTCATGCACCTAACACGAGCCGGCGGCAAACGATTCCGCCCCATGTTTGCA
CTGCTGGCCTCCGAGTTCGGTGAAAAACCACTCTCCGAAAACGTCATCAAAGCCGCCGTTGTCGTAGAGATCACCCACCT
GGCCACCCTGTACCACGACGATGTCATGGACGAGGCATCCATGCGCCGCGGCGTCCCAAGTGCTAACGCGCGATGGGACA
ACTCCGTAGCCATCCTCGCAGGCGACATCCTCCTAGCACATGCATCAGGTCTGATGAGTCAGCTGGGTACCGACACAGTC
GCCCACTTTGCCGAAACATTCGGCGAACTAGTCACCGGCCAAATGCGCGAAACAGTCGGGCCACGCGACACCGACCCGAT
CGAGCACTACACCAACGTAATCCGTGAAAAAACTGGTGTCCTCATCGCCTCCGCAGGCTATTTGGGAGCCATGCACGCAG
GCGCCGCACCTGAACACATCGACGCCCTGAAGAACTTCGGCGCAGCCGTCGGCATGATCTTCCAAATCGTCGACGACATC
ATCGACATCTTCTCGGAAACCCACGAATCCGGAAAAACGCCCGGCACCGACCTCCGCGAAGGTGTATTCACCCTCCCAGT
GCTCTACGCACTCCGTGAAGACACCCCCGTCGGCGCAGAACTCCGCGACATCCTCACCGGCCCTCTAGAAGACGACGAGA
CCGTCAACCACGTCCTCGAGCTCCTCTCCCAATCCGGCGGACGCCAAGCAGCCCTCGACGAGGTCTACCGCTACATGGAC
ATCGCCAACGCAGAACTCGACCGCCTCCCAGACAGCACCGTCAAGGAAGCCCTCCGCAACCTTGCAACCTTCACAGTCAA
GCGCGTCGGATAA

Upstream 100 bases:

>100_bases
GTGCCTTCCGTCGACTACGGTTAAACAAAAAGCTTTTTGTCCATTTCACTGGATTCACCGAAAGAATGAATCCACACTCG
ATCACCAAAGGTAGCGATGA

Downstream 100 bases:

>100_bases
CCCCGTAATCCACCACTTAACCTGCCGATTTGCATAACAGGCGGGGTCTCATAGTAAAGTATGAGGCGCACCAAACGAGG
TGCCCGCCAGATTGCCCGAG

Product: octaprenyl-diphosphate synthase protein

Products: NA

Alternate protein names: HEPPP synthase subunit 2 [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSSGRTVPTRSHGLGKEGVSTTGASQVEFGDPELTARINDAMVQVEELLHTELSSGEDFLVDIVMHLTRAGGKRFRPMFA
LLASEFGEKPLSENVIKAAVVVEITHLATLYHDDVMDEASMRRGVPSANARWDNSVAILAGDILLAHASGLMSQLGTDTV
AHFAETFGELVTGQMRETVGPRDTDPIEHYTNVIREKTGVLIASAGYLGAMHAGAAPEHIDALKNFGAAVGMIFQIVDDI
IDIFSETHESGKTPGTDLREGVFTLPVLYALREDTPVGAELRDILTGPLEDDETVNHVLELLSQSGGRQAALDEVYRYMD
IANAELDRLPDSTVKEALRNLATFTVKRVG

Sequences:

>Translated_350_residues
MSSGRTVPTRSHGLGKEGVSTTGASQVEFGDPELTARINDAMVQVEELLHTELSSGEDFLVDIVMHLTRAGGKRFRPMFA
LLASEFGEKPLSENVIKAAVVVEITHLATLYHDDVMDEASMRRGVPSANARWDNSVAILAGDILLAHASGLMSQLGTDTV
AHFAETFGELVTGQMRETVGPRDTDPIEHYTNVIREKTGVLIASAGYLGAMHAGAAPEHIDALKNFGAAVGMIFQIVDDI
IDIFSETHESGKTPGTDLREGVFTLPVLYALREDTPVGAELRDILTGPLEDDETVNHVLELLSQSGGRQAALDEVYRYMD
IANAELDRLPDSTVKEALRNLATFTVKRVG
>Mature_349_residues
SSGRTVPTRSHGLGKEGVSTTGASQVEFGDPELTARINDAMVQVEELLHTELSSGEDFLVDIVMHLTRAGGKRFRPMFAL
LASEFGEKPLSENVIKAAVVVEITHLATLYHDDVMDEASMRRGVPSANARWDNSVAILAGDILLAHASGLMSQLGTDTVA
HFAETFGELVTGQMRETVGPRDTDPIEHYTNVIREKTGVLIASAGYLGAMHAGAAPEHIDALKNFGAAVGMIFQIVDDII
DIFSETHESGKTPGTDLREGVFTLPVLYALREDTPVGAELRDILTGPLEDDETVNHVLELLSQSGGRQAALDEVYRYMDI
ANAELDRLPDSTVKEALRNLATFTVKRVG

Specific function: Supplies heptaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinone menaquinone-7 (MQ-7) [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=286, Percent_Identity=30.0699300699301, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1789578, Length=318, Percent_Identity=33.0188679245283, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1786623, Length=219, Percent_Identity=30.5936073059361, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=322, Percent_Identity=29.1925465838509, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319475, Length=298, Percent_Identity=29.8657718120805, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24651612, Length=288, Percent_Identity=30.2083333333333, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281365769, Length=257, Percent_Identity=27.2373540856031, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24660002, Length=257, Percent_Identity=27.2373540856031, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014119
- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.30 [H]

Molecular weight: Translated: 37823; Mature: 37692

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGRTVPTRSHGLGKEGVSTTGASQVEFGDPELTARINDAMVQVEELLHTELSSGEDFL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VDIVMHLTRAGGKRFRPMFALLASEFGEKPLSENVIKAAVVVEITHLATLYHDDVMDEAS
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRRGVPSANARWDNSVAILAGDILLAHASGLMSQLGTDTVAHFAETFGELVTGQMRETVG
HHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCC
PRDTDPIEHYTNVIREKTGVLIASAGYLGAMHAGAAPEHIDALKNFGAAVGMIFQIVDDI
CCCCCHHHHHHHHHHHHHCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IDIFSETHESGKTPGTDLREGVFTLPVLYALREDTPVGAELRDILTGPLEDDETVNHVLE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHH
LLSQSGGRQAALDEVYRYMDIANAELDRLPDSTVKEALRNLATFTVKRVG
HHHCCCCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSGRTVPTRSHGLGKEGVSTTGASQVEFGDPELTARINDAMVQVEELLHTELSSGEDFL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VDIVMHLTRAGGKRFRPMFALLASEFGEKPLSENVIKAAVVVEITHLATLYHDDVMDEAS
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRRGVPSANARWDNSVAILAGDILLAHASGLMSQLGTDTVAHFAETFGELVTGQMRETVG
HHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCC
PRDTDPIEHYTNVIREKTGVLIASAGYLGAMHAGAAPEHIDALKNFGAAVGMIFQIVDDI
CCCCCHHHHHHHHHHHHHCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IDIFSETHESGKTPGTDLREGVFTLPVLYALREDTPVGAELRDILTGPLEDDETVNHVLE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHH
LLSQSGGRQAALDEVYRYMDIANAELDRLPDSTVKEALRNLATFTVKRVG
HHHCCCCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7629164 [H]