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The map label for this gene is engD [H]

Identifier: 62185076

GI number: 62185076

Start: 514765

End: 515859

Strand: Direct

Name: engD [H]

Synonym: CAB447

Alternate gene names: 62185076

Gene position: 514765-515859 (Clockwise)

Preceding gene: 62185067

Following gene: 62185083

Centisome position: 44.98

GC content: 40.91

Gene sequence:

>1095_bases
ATGGGGCATACAGAATGTGGCATTGTAGGACTTCCCAATGTAGGTAAGTCTGGGTTATTTAATGCTCTTACTGGAGCGCA
AGTAGCTTCTTGTAACTATCCCTTTTGCACAATTGACCCGAACATTGGTATTGTTCCGGTTATAGATAACCGTCTAGATA
TTTTAGCTAAGATGAGTCAGAGTCAGAAGGTGATCTATGCCGATATGAAATTTGTTGATATTGCTGGTTTGGTGAAGGGA
GCTGCAGACGGAGCAGGATTAGGAAATCGGTTTCTCTCTCATATCCGTGAGACTCATGCTGTGGCTCATGTTGTTCGTTG
TTTTGACAATGATGATGTGACGCATGTATCGGGAAAGATAGATCCCAGTGATGACATTTCTGTGATAAATTTAGAATTGA
TCTTTTCTGATTTTTCTTCGGCAACAAATATTCATAGCAAATTAGAGAAACAAGCGAAGGGCAAGAAAGACCTTGGGATG
GTTTTACCTTTACTTGATAGAGTGATTAAACATTTAGAAAGTGGTCAACCCGTTCGTACTTTACATTTATCTTCTGAAGA
GCAGATACAACTCAAACCTTATCCCTTTTTAACAGCAAAACCTATGTTGTATGTAGCGAACATAGGCGAGGATTCCATAG
CCACTATGCATAACGATTATGTTGCTGTTGTTCAAGAAATTGCGAAAAAAGAAAATGCTCCTGTCGTTCCTATTTGTGTA
CAGTTAGAAGAAGAAATCATTTCGCTTCCTGTAGAAGAACGGCAAGACTTTTTAAATAGTTTGGGGCTAAAAGAATCGGG
ATTACATCGGTTAGTTCGTGCTGCTTACCATACACTTGGATTGATTTCTTATTTTACAACTGGACCACAAGAAACCCGAG
CTTGGACTATTCCTATAGGATCTACAGCTGCGGAAGCTGCAGGCCAAATTCACACCGATATCCAAAAAGGGTTTATCCGA
GCGGAAGTCGTTACTCTTGAAGACATGATCACCTATGGAAGCCGTACAGGAGTCCGCGAAGCCGGCAAATTGCGAGCAGA
GGGTAGAGACTACATTGTGCAGGATGGCGATATTATGCTTTTCTTGCATAATTAG

Upstream 100 bases:

>100_bases
CGAATGCAAGCTCCCACAAGCATCGAATCAACGATTCTTATGTTTGTGGGAATTCTTCCCTAGTAAAGGTAATATTAAAA
ATAGCTACGAAAGATAACAT

Downstream 100 bases:

>100_bases
TAGAGAAGAAAGCTTTTGCGCTGTCTATGTCCTTTCGAATTGCTTGAGACAACATCTCTCGAGAAGGGAATTTTCTTTCT
TTGCGGATAAATTTCTTAGG

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MGHTECGIVGLPNVGKSGLFNALTGAQVASCNYPFCTIDPNIGIVPVIDNRLDILAKMSQSQKVIYADMKFVDIAGLVKG
AADGAGLGNRFLSHIRETHAVAHVVRCFDNDDVTHVSGKIDPSDDISVINLELIFSDFSSATNIHSKLEKQAKGKKDLGM
VLPLLDRVIKHLESGQPVRTLHLSSEEQIQLKPYPFLTAKPMLYVANIGEDSIATMHNDYVAVVQEIAKKENAPVVPICV
QLEEEIISLPVEERQDFLNSLGLKESGLHRLVRAAYHTLGLISYFTTGPQETRAWTIPIGSTAAEAAGQIHTDIQKGFIR
AEVVTLEDMITYGSRTGVREAGKLRAEGRDYIVQDGDIMLFLHN

Sequences:

>Translated_364_residues
MGHTECGIVGLPNVGKSGLFNALTGAQVASCNYPFCTIDPNIGIVPVIDNRLDILAKMSQSQKVIYADMKFVDIAGLVKG
AADGAGLGNRFLSHIRETHAVAHVVRCFDNDDVTHVSGKIDPSDDISVINLELIFSDFSSATNIHSKLEKQAKGKKDLGM
VLPLLDRVIKHLESGQPVRTLHLSSEEQIQLKPYPFLTAKPMLYVANIGEDSIATMHNDYVAVVQEIAKKENAPVVPICV
QLEEEIISLPVEERQDFLNSLGLKESGLHRLVRAAYHTLGLISYFTTGPQETRAWTIPIGSTAAEAAGQIHTDIQKGFIR
AEVVTLEDMITYGSRTGVREAGKLRAEGRDYIVQDGDIMLFLHN
>Mature_363_residues
GHTECGIVGLPNVGKSGLFNALTGAQVASCNYPFCTIDPNIGIVPVIDNRLDILAKMSQSQKVIYADMKFVDIAGLVKGA
ADGAGLGNRFLSHIRETHAVAHVVRCFDNDDVTHVSGKIDPSDDISVINLELIFSDFSSATNIHSKLEKQAKGKKDLGMV
LPLLDRVIKHLESGQPVRTLHLSSEEQIQLKPYPFLTAKPMLYVANIGEDSIATMHNDYVAVVQEIAKKENAPVVPICVQ
LEEEIISLPVEERQDFLNSLGLKESGLHRLVRAAYHTLGLISYFTTGPQETRAWTIPIGSTAAEAAGQIHTDIQKGFIRA
EVVTLEDMITYGSRTGVREAGKLRAEGRDYIVQDGDIMLFLHN

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=366, Percent_Identity=43.9890710382514, Blast_Score=294, Evalue=9e-80,
Organism=Homo sapiens, GI58761502, Length=196, Percent_Identity=40.8163265306122, Blast_Score=156, Evalue=3e-38,
Organism=Escherichia coli, GI1787454, Length=361, Percent_Identity=53.4626038781163, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1789574, Length=129, Percent_Identity=32.5581395348837, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17509631, Length=365, Percent_Identity=41.0958904109589, Blast_Score=275, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6319499, Length=367, Percent_Identity=38.9645776566758, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6321773, Length=381, Percent_Identity=33.5958005249344, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321649, Length=140, Percent_Identity=37.8571428571429, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24640873, Length=366, Percent_Identity=39.8907103825137, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24640877, Length=366, Percent_Identity=39.8907103825137, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24640875, Length=366, Percent_Identity=39.8907103825137, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24640879, Length=328, Percent_Identity=37.8048780487805, Blast_Score=212, Evalue=3e-55,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 39783; Mature: 39652

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHTECGIVGLPNVGKSGLFNALTGAQVASCNYPFCTIDPNIGIVPVIDNRLDILAKMSQ
CCCCCCCEEECCCCCCCHHHHHHCCCEEEECCCCEEEECCCCCEEEEECCHHHHHHHHCC
SQKVIYADMKFVDIAGLVKGAADGAGLGNRFLSHIRETHAVAHVVRCFDNDDVTHVSGKI
CCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
DPSDDISVINLELIFSDFSSATNIHSKLEKQAKGKKDLGMVLPLLDRVIKHLESGQPVRT
CCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEE
LHLSSEEQIQLKPYPFLTAKPMLYVANIGEDSIATMHNDYVAVVQEIAKKENAPVVPICV
EECCCCCCEEECCCCCEECCCEEEEEECCCCHHHHHCCHHHHHHHHHHHHCCCCEEEEEH
QLEEEIISLPVEERQDFLNSLGLKESGLHRLVRAAYHTLGLISYFTTGPQETRAWTIPIG
HHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
STAAEAAGQIHTDIQKGFIRAEVVTLEDMITYGSRTGVREAGKLRAEGRDYIVQDGDIML
CHHHHHHHHHHHHHHHCHHHHEEEEHHHHHHHCCCCCCHHHHHHCCCCCCEEEECCCEEE
FLHN
EEEC
>Mature Secondary Structure 
GHTECGIVGLPNVGKSGLFNALTGAQVASCNYPFCTIDPNIGIVPVIDNRLDILAKMSQ
CCCCCCEEECCCCCCCHHHHHHCCCEEEECCCCEEEECCCCCEEEEECCHHHHHHHHCC
SQKVIYADMKFVDIAGLVKGAADGAGLGNRFLSHIRETHAVAHVVRCFDNDDVTHVSGKI
CCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
DPSDDISVINLELIFSDFSSATNIHSKLEKQAKGKKDLGMVLPLLDRVIKHLESGQPVRT
CCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEE
LHLSSEEQIQLKPYPFLTAKPMLYVANIGEDSIATMHNDYVAVVQEIAKKENAPVVPICV
EECCCCCCEEECCCCCEECCCEEEEEECCCCHHHHHCCHHHHHHHHHHHHCCCCEEEEEH
QLEEEIISLPVEERQDFLNSLGLKESGLHRLVRAAYHTLGLISYFTTGPQETRAWTIPIG
HHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
STAAEAAGQIHTDIQKGFIRAEVVTLEDMITYGSRTGVREAGKLRAEGRDYIVQDGDIML
CHHHHHHHHHHHHHHHCHHHHEEEEHHHHHHHCCCCCCHHHHHHCCCCCCEEEECCCEEE
FLHN
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]