Click here to switch to the map view.

The map label for this gene is dapF [H]

Identifier: 62184865

GI number: 62184865

Start: 242986

End: 243768

Strand: Direct

Name: dapF [H]

Synonym: CAB223

Alternate gene names: 62184865

Gene position: 242986-243768 (Clockwise)

Preceding gene: 62184864

Following gene: 62184866

Centisome position: 21.23

GC content: 43.81

Gene sequence:

>783_bases
ATGGCATCCTCTTCTCATTCAATGATTTATAAGCCTAGCTTATATTCTGGGGCAGGGAATCGCTTTATCCTAAGTGAAAC
TTGTCCCGATATGACAATACTTCCTAGTCTATGTAAGGAATATCAAGTAGATGGGTTTTTACTTGTTCTCCCTTCTTCTG
TTGCTGATGCTAAACTGATTATTTTTAATGATAACGGTTCTCGCCCTCCGATGTGTGGGAACGGTTTGCGTTGTGTTATC
GCTCATGTCTCTCAGGTAGGGAAAAAAGACCACATCTTTGTAGAGACAGATTCGGGTATATATTCTGGGAGGTTTGAGTC
TTGGGAGCGGGTTATTGTAGATATGACTCTTCCGGATTGGCACTACACTCGTCACCTCCTTTCACACACACTTCCGGGAG
TTCCTAAAGAAGTGTTTAGTATCAATACAGGGGTGCCGCATCTTGTTGTTTTTGTCGAAGATGTCTCATGTGTTCCTGTA
GATTTGTGGGGGAGTTTTCTACGGTATCATGAAGACTTTGTGCCCCAAGGGACTAATGTCAACTTTATCCAGGAGATATC
AACTGGAGAATTTCGCATACGCACGTATGAACGTGGCTTGGAAAGAGAGTCTTTAGCTTGTGGTACGGGAGCTACGGCAG
CGGCGTTAGTTGTAGCTCGACGTTACGGTTTATCCAATACCCAAATACGTATACGGACCTGGAGTGACGTGTTGATGAAA
ATTTCTTTAGATAGTGATCGAGTGTATCTTGAGGGCCATGTGGATAAGGAAATGCCCGACTAG

Upstream 100 bases:

>100_bases
TATTTAGAGGCGACCGGGCAGTCTCGAGAAGTGATTGAAAAGGCCATCGATCGAGACACTTGGATGACAGCAGACGAAGC
CAAAGATTTTGGTTTATTAG

Downstream 100 bases:

>100_bases
AGCATATCGAATTTTTTATTGAATTTGAATAAAAATAGATTCAAAAATCCTTAAAGGAAGGAAAGGGATTGGAGGTTTTT
TAAGGATAGACCTCTTGCCC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MASSSHSMIYKPSLYSGAGNRFILSETCPDMTILPSLCKEYQVDGFLLVLPSSVADAKLIIFNDNGSRPPMCGNGLRCVI
AHVSQVGKKDHIFVETDSGIYSGRFESWERVIVDMTLPDWHYTRHLLSHTLPGVPKEVFSINTGVPHLVVFVEDVSCVPV
DLWGSFLRYHEDFVPQGTNVNFIQEISTGEFRIRTYERGLERESLACGTGATAAALVVARRYGLSNTQIRIRTWSDVLMK
ISLDSDRVYLEGHVDKEMPD

Sequences:

>Translated_260_residues
MASSSHSMIYKPSLYSGAGNRFILSETCPDMTILPSLCKEYQVDGFLLVLPSSVADAKLIIFNDNGSRPPMCGNGLRCVI
AHVSQVGKKDHIFVETDSGIYSGRFESWERVIVDMTLPDWHYTRHLLSHTLPGVPKEVFSINTGVPHLVVFVEDVSCVPV
DLWGSFLRYHEDFVPQGTNVNFIQEISTGEFRIRTYERGLERESLACGTGATAAALVVARRYGLSNTQIRIRTWSDVLMK
ISLDSDRVYLEGHVDKEMPD
>Mature_259_residues
ASSSHSMIYKPSLYSGAGNRFILSETCPDMTILPSLCKEYQVDGFLLVLPSSVADAKLIIFNDNGSRPPMCGNGLRCVIA
HVSQVGKKDHIFVETDSGIYSGRFESWERVIVDMTLPDWHYTRHLLSHTLPGVPKEVFSINTGVPHLVVFVEDVSCVPVD
LWGSFLRYHEDFVPQGTNVNFIQEISTGEFRIRTYERGLERESLACGTGATAAALVVARRYGLSNTQIRIRTWSDVLMKI
SLDSDRVYLEGHVDKEMPD

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=182, Percent_Identity=31.3186813186813, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29091; Mature: 28959

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSSHSMIYKPSLYSGAGNRFILSETCPDMTILPSLCKEYQVDGFLLVLPSSVADAKLI
CCCCCCCEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEE
IFNDNGSRPPMCGNGLRCVIAHVSQVGKKDHIFVETDSGIYSGRFESWERVIVDMTLPDW
EECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEECCCCCCCEEEEEEEECCCH
HYTRHLLSHTLPGVPKEVFSINTGVPHLVVFVEDVSCVPVDLWGSFLRYHEDFVPQGTNV
HHHHHHHHHCCCCCCHHHEEECCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHCCCCCCC
NFIQEISTGEFRIRTYERGLERESLACGTGATAAALVVARRYGLSNTQIRIRTWSDVLMK
EEEEECCCCCEEEEEHHCCCCCCCEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCEEEE
ISLDSDRVYLEGHVDKEMPD
EEECCCEEEEEECCCCCCCC
>Mature Secondary Structure 
ASSSHSMIYKPSLYSGAGNRFILSETCPDMTILPSLCKEYQVDGFLLVLPSSVADAKLI
CCCCCCEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEE
IFNDNGSRPPMCGNGLRCVIAHVSQVGKKDHIFVETDSGIYSGRFESWERVIVDMTLPDW
EECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEECCCCCCCEEEEEEEECCCH
HYTRHLLSHTLPGVPKEVFSINTGVPHLVVFVEDVSCVPVDLWGSFLRYHEDFVPQGTNV
HHHHHHHHHCCCCCCHHHEEECCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHCCCCCCC
NFIQEISTGEFRIRTYERGLERESLACGTGATAAALVVARRYGLSNTQIRIRTWSDVLMK
EEEEECCCCCEEEEEHHCCCCCCCEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCEEEE
ISLDSDRVYLEGHVDKEMPD
EEECCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]