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The map label for this gene is ypfI [H]

Identifier: 59714352

GI number: 59714352

Start: 1325198

End: 1327327

Strand: Reverse

Name: ypfI [H]

Synonym: VF_A1169

Alternate gene names: 59714352

Gene position: 1327327-1325198 (Counterclockwise)

Preceding gene: 59714354

Following gene: 59714351

Centisome position: 99.77

GC content: 36.62

Gene sequence:

>2130_bases
ATGGATCTTCCCATGCCTCAAGTTTTATCTTTTTTGAAAACAGTTTCTGCTTATGCAGCGAGCGGTTTTATTCGATATCC
AATTCTTTTAGAAGGTTCTATCGGTTGGAGCGAAGAAATCATTAAAGAGTATATTGCTCATTCTGAATATCTAGATTCTG
ATATTGAGGTTTACGGTAACCTCTCTATTGGTGAATTACCTAGCTTAGAAATGAAAACTGCACTGAAAAAGTTAGGACAA
GAAACACATTGTTTGGTGATTGATATTTCTCATGAGTTTAATGCGAATGCAATAAATGCAGTTTGTGGAACACTTGTTGG
TGGGGGATTAGTTTTTTTTATTCGCTCTGAAGACTCATCTTTGCATCGTAATATTGAACGTTGGTTAACACACTTTAAAA
AGCAGATGCTCGTTATTTCAGAAAATGCTCCTTTGCCGTTACTACCGCAGGTGCCAACAGAAAAAAAGTTAACGTCGTCT
TTTACTCAGACAGATAAATTAGATGAACTATATCGTTCTATCGATCAAAAGCATGCCGTGTTAAGTATACAAAGAGTGAT
TACTGGCCACGCTAAGAGACCATTAATTATCACCGCTGATCGTGGACGAGGAAAATCTTCAGCAATAGGTATCGCTATCG
CTGAGCTTGTTCGTGATAAAAAATTGGTTATTGGTATTACGGCACCACTACGCGTGAATATCAATGAAGTTTTCGGTCAT
GCTGCAAGTGTGGCAAAAAAATTAGGAATGCCATTTAAACAAGAAAAAAACACCTTAACTATAAATGGCTCAACGGTTAC
GTATTTTGCTCCGGATGAATTAATACGTGATTCTTATTCATTAGATTTGTTATGTATCGATGAAGCGGCAGCTATTCCTG
CACCGATGTTATTGGCATTAACTGAGCGTTATTCTCGTCTGGTTTTTTCTACCACAATTAATGGTTATGAAGGGACTGGT
AGAGGATTCGAAATAAAGTTCAAAAAGCAACTTGCTCTGCTACGCCCAAACTTTCATTCTGTGGAAATGATACAACCTAT
TCGCTGGTCTGAACTCGATCCATTAGAAAATTGGTTATCTTCTGTTTTTTTATTAGATCACAAAGTGTCGAGTAATAAGC
AACTGGATATTAGCCGAGAGGATGTCGAATTTGTTCGCTTGAATGTAAGTGATTTATTAACGGATCCAGAATTAGCACAA
TCTATTTTTTCGTTATTAGTGTCAGCTCACTATCAAACATCACCTAATGATTGGTTGGCTCTATTATCTGATCCCAGTCT
TTTTTGTTGGGCTGGTATAGATAAGTCGTCTAGAAATGTACTTACATGCGCATTAGTTTCTTTGGAAGGTCAGTTAGATA
ACGAGCTTATTGCTGATATTCAAGTTGGCAAAAGAAGACCTAGAGGACATTTAGTTCCTGTCTCGTTAACACAAACATTA
TGCATTAACGAACCCGCTCAACAATCTTGTATTCGTATTATGAGAATTGCGGTTGAGCAACATTATCAACGTATGCATCT
TGGTTCTAAATTCATTGAGTATATTAAAGCGTTCTATTGTGATTTGGGTATTGATTACTTAAGCACGAGTTTTGGTTCCA
CTCCTGGATTGAATGAATTTTGGTTGAGCTTAGATTTTAGTTTTATGCGATTAGGTATTTCTAAGGATAAAGCGAGCGGA
AGCCACTCGTTACTGGCAGTATTGCCTTTATCCAAAAATGCTATCGAATGGCTACCTCTTGGTAAAGATTATTTTGCTAT
GGCTTTACCTGAGCAATTACTAAGTGTTTTTAATGAACTTGATGTTTCTGAAGTATTAAGTTTATTAAAAGGGATCGATA
CGAAACAGCCGTTAGCCGATGTAATGATTAAAAGAATTGAAATGTATTCTAACGGTGGGTTAGGCGTTGAGATGATTCAG
TATGAACTCAGACATTTTTTATTGTCGTCTATATCACTTCTGTTAACTCTAAATGCAACGCACCAAGTATTAGTGGTATC
TAAGATTCTTCAATTACAAGATTGGAAAACTAGCATTTCTCTTTCAAAGGTTTCAGGAAGAGGAGAAGCCGAAACTGAAA
TGCGCTCAGCAATTAGAATCTTATTGTGCAATTTACACTGTAAATCCTAG

Upstream 100 bases:

>100_bases
TATTATACCTTTTCAAATAAACTCTCGTCATACTTTCTTTTCTTAATTAAAGGAAACTTTGTATCTCATGCTACAATTCA
AAGGTTTTTATCTCTCGTAA

Downstream 100 bases:

>100_bases
TCAAATACTATAGTCATCAATTTACAGTGTAAATTAAGATTTTCATATTCATTGAATTTACACTGTAAATCCCATCTAAA
GAGTTATCCTAAATTTGATT

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MDLPMPQVLSFLKTVSAYAASGFIRYPILLEGSIGWSEEIIKEYIAHSEYLDSDIEVYGNLSIGELPSLEMKTALKKLGQ
ETHCLVIDISHEFNANAINAVCGTLVGGGLVFFIRSEDSSLHRNIERWLTHFKKQMLVISENAPLPLLPQVPTEKKLTSS
FTQTDKLDELYRSIDQKHAVLSIQRVITGHAKRPLIITADRGRGKSSAIGIAIAELVRDKKLVIGITAPLRVNINEVFGH
AASVAKKLGMPFKQEKNTLTINGSTVTYFAPDELIRDSYSLDLLCIDEAAAIPAPMLLALTERYSRLVFSTTINGYEGTG
RGFEIKFKKQLALLRPNFHSVEMIQPIRWSELDPLENWLSSVFLLDHKVSSNKQLDISREDVEFVRLNVSDLLTDPELAQ
SIFSLLVSAHYQTSPNDWLALLSDPSLFCWAGIDKSSRNVLTCALVSLEGQLDNELIADIQVGKRRPRGHLVPVSLTQTL
CINEPAQQSCIRIMRIAVEQHYQRMHLGSKFIEYIKAFYCDLGIDYLSTSFGSTPGLNEFWLSLDFSFMRLGISKDKASG
SHSLLAVLPLSKNAIEWLPLGKDYFAMALPEQLLSVFNELDVSEVLSLLKGIDTKQPLADVMIKRIEMYSNGGLGVEMIQ
YELRHFLLSSISLLLTLNATHQVLVVSKILQLQDWKTSISLSKVSGRGEAETEMRSAIRILLCNLHCKS

Sequences:

>Translated_709_residues
MDLPMPQVLSFLKTVSAYAASGFIRYPILLEGSIGWSEEIIKEYIAHSEYLDSDIEVYGNLSIGELPSLEMKTALKKLGQ
ETHCLVIDISHEFNANAINAVCGTLVGGGLVFFIRSEDSSLHRNIERWLTHFKKQMLVISENAPLPLLPQVPTEKKLTSS
FTQTDKLDELYRSIDQKHAVLSIQRVITGHAKRPLIITADRGRGKSSAIGIAIAELVRDKKLVIGITAPLRVNINEVFGH
AASVAKKLGMPFKQEKNTLTINGSTVTYFAPDELIRDSYSLDLLCIDEAAAIPAPMLLALTERYSRLVFSTTINGYEGTG
RGFEIKFKKQLALLRPNFHSVEMIQPIRWSELDPLENWLSSVFLLDHKVSSNKQLDISREDVEFVRLNVSDLLTDPELAQ
SIFSLLVSAHYQTSPNDWLALLSDPSLFCWAGIDKSSRNVLTCALVSLEGQLDNELIADIQVGKRRPRGHLVPVSLTQTL
CINEPAQQSCIRIMRIAVEQHYQRMHLGSKFIEYIKAFYCDLGIDYLSTSFGSTPGLNEFWLSLDFSFMRLGISKDKASG
SHSLLAVLPLSKNAIEWLPLGKDYFAMALPEQLLSVFNELDVSEVLSLLKGIDTKQPLADVMIKRIEMYSNGGLGVEMIQ
YELRHFLLSSISLLLTLNATHQVLVVSKILQLQDWKTSISLSKVSGRGEAETEMRSAIRILLCNLHCKS
>Mature_709_residues
MDLPMPQVLSFLKTVSAYAASGFIRYPILLEGSIGWSEEIIKEYIAHSEYLDSDIEVYGNLSIGELPSLEMKTALKKLGQ
ETHCLVIDISHEFNANAINAVCGTLVGGGLVFFIRSEDSSLHRNIERWLTHFKKQMLVISENAPLPLLPQVPTEKKLTSS
FTQTDKLDELYRSIDQKHAVLSIQRVITGHAKRPLIITADRGRGKSSAIGIAIAELVRDKKLVIGITAPLRVNINEVFGH
AASVAKKLGMPFKQEKNTLTINGSTVTYFAPDELIRDSYSLDLLCIDEAAAIPAPMLLALTERYSRLVFSTTINGYEGTG
RGFEIKFKKQLALLRPNFHSVEMIQPIRWSELDPLENWLSSVFLLDHKVSSNKQLDISREDVEFVRLNVSDLLTDPELAQ
SIFSLLVSAHYQTSPNDWLALLSDPSLFCWAGIDKSSRNVLTCALVSLEGQLDNELIADIQVGKRRPRGHLVPVSLTQTL
CINEPAQQSCIRIMRIAVEQHYQRMHLGSKFIEYIKAFYCDLGIDYLSTSFGSTPGLNEFWLSLDFSFMRLGISKDKASG
SHSLLAVLPLSKNAIEWLPLGKDYFAMALPEQLLSVFNELDVSEVLSLLKGIDTKQPLADVMIKRIEMYSNGGLGVEMIQ
YELRHFLLSSISLLLTLNATHQVLVVSKILQLQDWKTSISLSKVSGRGEAETEMRSAIRILLCNLHCKS

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP) [H]

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI221316741, Length=442, Percent_Identity=28.9592760180996, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI221316723, Length=442, Percent_Identity=28.9592760180996, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI1788818, Length=590, Percent_Identity=28.6440677966102, Blast_Score=185, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17507621, Length=401, Percent_Identity=29.925187032419, Blast_Score=129, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6324197, Length=578, Percent_Identity=26.2975778546713, Blast_Score=126, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24640662, Length=561, Percent_Identity=27.0944741532977, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR007807 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF05127 DUF699 [H]

EC number: NA

Molecular weight: Translated: 79211; Mature: 79211

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLPMPQVLSFLKTVSAYAASGFIRYPILLEGSIGWSEEIIKEYIAHSEYLDSDIEVYGN
CCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEC
LSIGELPSLEMKTALKKLGQETHCLVIDISHEFNANAINAVCGTLVGGGLVFFIRSEDSS
CCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCH
LHRNIERWLTHFKKQMLVISENAPLPLLPQVPTEKKLTSSFTQTDKLDELYRSIDQKHAV
HHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSIQRVITGHAKRPLIITADRGRGKSSAIGIAIAELVRDKKLVIGITAPLRVNINEVFGH
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCEEECHHHHHHH
AASVAKKLGMPFKQEKNTLTINGSTVTYFAPDELIRDSYSLDLLCIDEAAAIPAPMLLAL
HHHHHHHHCCCCCCCCCEEEEECCEEEEECCHHHHCCCCCEEEEEECCCCCCCHHHHHHH
TERYSRLVFSTTINGYEGTGRGFEIKFKKQLALLRPNFHSVEMIQPIRWSELDPLENWLS
HHHHHHEEEEEEECCCCCCCCCEEEEEHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHHHH
SVFLLDHKVSSNKQLDISREDVEFVRLNVSDLLTDPELAQSIFSLLVSAHYQTSPNDWLA
HHHHHEECCCCCCCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEE
LLSDPSLFCWAGIDKSSRNVLTCALVSLEGQLDNELIADIQVGKRRPRGHLVPVSLTQTL
EECCCCEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHH
CINEPAQQSCIRIMRIAVEQHYQRMHLGSKFIEYIKAFYCDLGIDYLSTSFGSTPGLNEF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHH
WLSLDFSFMRLGISKDKASGSHSLLAVLPLSKNAIEWLPLGKDYFAMALPEQLLSVFNEL
HHEECHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCC
DVSEVLSLLKGIDTKQPLADVMIKRIEMYSNGGLGVEMIQYELRHFLLSSISLLLTLNAT
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCC
HQVLVVSKILQLQDWKTSISLSKVSGRGEAETEMRSAIRILLCNLHCKS
HHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDLPMPQVLSFLKTVSAYAASGFIRYPILLEGSIGWSEEIIKEYIAHSEYLDSDIEVYGN
CCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEC
LSIGELPSLEMKTALKKLGQETHCLVIDISHEFNANAINAVCGTLVGGGLVFFIRSEDSS
CCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCH
LHRNIERWLTHFKKQMLVISENAPLPLLPQVPTEKKLTSSFTQTDKLDELYRSIDQKHAV
HHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSIQRVITGHAKRPLIITADRGRGKSSAIGIAIAELVRDKKLVIGITAPLRVNINEVFGH
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCEEECHHHHHHH
AASVAKKLGMPFKQEKNTLTINGSTVTYFAPDELIRDSYSLDLLCIDEAAAIPAPMLLAL
HHHHHHHHCCCCCCCCCEEEEECCEEEEECCHHHHCCCCCEEEEEECCCCCCCHHHHHHH
TERYSRLVFSTTINGYEGTGRGFEIKFKKQLALLRPNFHSVEMIQPIRWSELDPLENWLS
HHHHHHEEEEEEECCCCCCCCCEEEEEHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHHHH
SVFLLDHKVSSNKQLDISREDVEFVRLNVSDLLTDPELAQSIFSLLVSAHYQTSPNDWLA
HHHHHEECCCCCCCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEE
LLSDPSLFCWAGIDKSSRNVLTCALVSLEGQLDNELIADIQVGKRRPRGHLVPVSLTQTL
EECCCCEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHH
CINEPAQQSCIRIMRIAVEQHYQRMHLGSKFIEYIKAFYCDLGIDYLSTSFGSTPGLNEF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHH
WLSLDFSFMRLGISKDKASGSHSLLAVLPLSKNAIEWLPLGKDYFAMALPEQLLSVFNEL
HHEECHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCC
DVSEVLSLLKGIDTKQPLADVMIKRIEMYSNGGLGVEMIQYELRHFLLSSISLLLTLNAT
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCC
HQVLVVSKILQLQDWKTSISLSKVSGRGEAETEMRSAIRILLCNLHCKS
HHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]